• Title/Summary/Keyword: Base editing

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Development of 3D Digital Map Editing System (3차원 수치지도 편집 시스템 개발)

  • Lee, Jae-Kee;Park, Ki-Surk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.3
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    • pp.239-247
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    • 2007
  • The 3D spatial information projects have been processed and utilized in varied fields. However, the research of the 3D digital map for a role of national base map is not enough. The draft maps, which are raw data for generating 2D digital map, shows problems in generating 3D digital map. The objective of this research is to develop 3D digital map editing system for modifying and editing of 3D digital map from 2D vector and raster information such as a draft map, 2D digital map, DEM, aerial photo and so forth. This 3D digital map editing system was designed to include data structure of geometric and attribute object under provision of ISO/TC211 and OGC standard. This system was developed to implement the function of 3D stereo editing based on stereo viewing, 3D view editing based on projective, and 3D spatial operation. Using this system, 3D digital maps were able to be successfully produced from not only existing draft maps but also modified or edited draft maps and then application results were compared and analyzed.

The Change in News Video Production Environment by the Introduction of Network Production System

  • Won, Sung-Hoon;Choi, Won-Ho;Kim, Chee-Yong
    • Journal of information and communication convergence engineering
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    • v.9 no.1
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    • pp.110-116
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    • 2011
  • The production environment of fast and accurate news video has been continuously changing together with technology advance. Especially, the editing of the news video advanced focusing on the non-linear system by the introduction of digital technology and the recording of news video advanced to 'tapeless' base. In this new news video production environment, the recorded news videos are transmitted, edited and archived through the NPS (Network Production System), which dramatically improves the efficiency of the news video production. However, there are some issues which need to be resolved in the introduction of NPS. The purpose of this research is to identify the necessity of NPS introduction in the changing news video production environment, explore the issues which should be resolved and suggest the proper way to introduce the NPS to broadcasting companies.

Genetic Variation in Exon 3 of Human Apo B mRNA Editing Protein (apobec-1) Gene

  • Hong, Seung-Ho;Song, Jung-Han;Kim, Jin-Q
    • Journal of Genetic Medicine
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    • v.3 no.1
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    • pp.15-19
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    • 1999
  • We have investigated the genetic variation in the human apo B mRNA editing protein (apobec-1) gene. Exon 3 of the apobec-1 gene was amplified by polymerase chain reaction. After detection of an additional band by single strand conformational polymorphism (SSCP) analysis, sequencing of the SSCP-shift sample revealed a single-base mutation. The mutation was a CGG transversion at codon 80 resulting in a lleRMet substitution. This substitution was confirmed by restriction fragment length polymorphism analysis since a Pvull site is abolished by the substitution. Population and family studies confirmed that the inheritance of the genotypes for apobec-1 gene polymorphism is controlled by two codominant alleles (P1 and P2). A significant difference in plasma triglyceride was detected among the different apobec-1 genotypes in the CAD patients (P<0.05). Our study could provide the basis for elucidating the interaction between genetic variation of the apobec-1 gene and disorders related to lipid metabolism.

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GML Document Editing and Translation System based on Vector Graphic (벡터 그래픽 기반의 GML 문서 편집 및 변환 시스템)

  • Kim, Chang-Su;Yeom, Sung-Keun;Jung, Hoe-Kyung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.13 no.6
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    • pp.1058-1064
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    • 2009
  • According to development of Information Technology and generalization of internet, practical use field of geography information became various. Therefor various Geographic Information System (GIS) constructed to manage geography information efficiently. However, geography information data of various form is depending on graphic authorizing tool of various form being not normalized each other. So, OGC(Open Geospatial Consortium) proposed GML(Geography Markup Language) that describe normalized geography information data that can apply mutually and W3C proposed SVG(Scalable Vector Graphics) of vector base. In this paper creates GML data of XML base for geography information data processing to vector graphic object, and design and implementation of GML document editing and translation system that define code converter that create GML document through created graphic objects and change vector graphic to logic structure of XML base.

GML document editing and translation system based on GML and vector graphic (GML과 벡터 그래픽 기반의 GML 문서 편집 및 변환 시스템)

  • Kim, Chang-Su;Cho, Young-Soon;Cho, Tae-Beom;Bang, Jin-Suk;Jung, Hoe-Kyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2009.05a
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    • pp.645-648
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    • 2009
  • According to development of Information Technology and generalization of internet, practical use field of geography information became various. Therefor various Geographic Information System (GIS) constructed to manage geography information efficiently. However, geography information data of various form is depending on graphic authorizing tool of various form being not normalized each other. So, OGC (Open Geospatial Consortium) proposed GML (Geography Markup Language) that describe normalized geography information data that can apply mutually and W3C proposed SVG (Scalable Vector Graphics) of vector base. In this paper creates GML data of XML base for geography information data processing to vector graphic object. and design and implementation of GML document editing and translation system that define code converter that create GML document through created graphic objects and change vector graphic to logic structure of XML base.

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Observation of Gene Edition by the Transient Expression of CRISPR-Cas9 System During the Development of Tomato Cotyledon (Agrobacterium을 이용한 토마토 떡잎에서 CRISPR-Cas9 시스템의 임시발현 시 토마토 떡잎 발달 단계에 따른 유전자교정 효율 변화)

  • Kim, Euyeon;Yang, So Hee;Park, Hyosun;Koo, Yeonjong
    • Korean Journal of Environmental Agriculture
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    • v.40 no.3
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    • pp.186-193
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    • 2021
  • BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11th, 15th, 19th, and 23rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

XML Schema Document Editing System (XML 스키마 문서편집 시스템)

  • 차원준;최일선;김창수;정회경
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2003.05a
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    • pp.285-288
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    • 2003
  • Electronic commerce that is constructed in existing and XML document that is used in e-Business field made out because based to DTD. However, XML applications that XML Schema is much after become Recommendation in W3C May, 2001 XML Schema real condition that is developed to base be. Selected XML Schema in ebXML Registry 2.0 by representative thing connected with this. If develop XML Schema, compare when develop using existent DTD and complexity by namespace or object-oriented concept etc. was increasing, and could programming by various method. XML Schema document that is used by ebXML Framework in treatise that see hereupon study about XML Schema document editing system that offer mastication and user interface that can edit efficiently do.

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Advanced Process Technique for Field Check Data Editing and Structured Editing on Digital Map Ver2.0, Applying Automatic Error Detection Method (자동 오류검출 방법을 적용한 수치지도 Ver2.0 정위치 및 구조화 편집 공정개선 연구)

  • Lee Jin Soo;Park Chang Taek;Park Ki Surk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.23 no.3
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    • pp.331-340
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    • 2005
  • Digital map is very important digital geographic information which is the base for various fields such as building and using the geographic information system (GIS), planing the regional development, and etc. Therefore, it needs high accuracy. Then we offer the advanced technique which minimizes errors on digital maps, using the automated inspection through the whole figures. In addition this new technique raises the economical efficiency as well as accuracy applying the automated error detection method which can recognize, search and classify errors automatically.

Research Trends of Generative Adversarial Networks and Image Generation and Translation (GAN 적대적 생성 신경망과 이미지 생성 및 변환 기술 동향)

  • Jo, Y.J.;Bae, K.M.;Park, J.Y.
    • Electronics and Telecommunications Trends
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    • v.35 no.4
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    • pp.91-102
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    • 2020
  • Recently, generative adversarial networks (GANs) is a field of research that has rapidly emerged wherein many studies conducted shows overwhelming results. Initially, this was at the level of imitating the training dataset. However, the GAN is currently useful in many fields, such as transformation of data categories, restoration of erased parts of images, copying facial expressions of humans, and creation of artworks depicting a dead painter's style. Although many outstanding research achievements have been attracting attention recently, GANs have encountered many challenges. First, they require a large memory facility for research. Second, there are still technical limitations in processing high-resolution images over 4K. Third, many GAN learning methods have a problem of instability in the training stage. However, recent research results show images that are difficult to distinguish whether they are real or fake, even with the naked eye, and the resolution of 4K and above is being developed. With the increase in image quality and resolution, many applications in the field of design and image and video editing are now available, including those that draw a photorealistic image as a simple sketch or easily modify unnecessary parts of an image or a video. In this paper, we discuss how GANs started, including the base architecture and latest technologies of GANs used in high-resolution, high-quality image creation, image and video editing, style translation, content transfer, and technology.

Advanced Bioremediation Strategies for Organophosphorus Compounds

  • Anish Kumar Sharma;Jyotsana Pandit
    • Microbiology and Biotechnology Letters
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    • v.51 no.4
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    • pp.374-389
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    • 2023
  • Organophosphorus (OP) pesticides, particularly malathion, parathion, diazinon, and chlorpyrifos, are widely used in both agricultural and residential contexts. This refractory quality is shared by certain organ phosphorus insecticides, and it may have unintended consequences for certain non-target soil species. Bioremediation cleans organic and inorganic contaminants using microbes and plants. Organophosphate-hydrolyzing enzymes can transform pesticide residues into non-hazardous byproducts and are increasingly being considered viable solutions to the problem of decontamination. When coupled with system analysis, the multi-omics technique produces important data for functional validation and genetic manipulation, both of which may be used to boost the efficiency of bioremediation systems. RNA-guided nucleases and RNA-guided base editors include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR), which are used to alter genes and edit genomes. The review sheds light on key knowledge gaps and suggests approaches to pesticide cleanup using a variety of microbe-assisted methods. Researches, ecologists, and decision-makers can all benefit from having a better understanding of the usefulness and application of systems biology and gene editing in bioremediation evaluations.