• International Journal of Oral Biology
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• v.32 no.4
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• pp.119-125
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• 2007

Dlx3 is a homeodomain protein and is known to play a role in development and differentiation of many tissues. Deletion of four base pairs in DLX3 (NT3198) is causally related to tricho-dento-osseous (TDO) syndrome (OMIM #190320), a genetic disorder manifested by taurodontism, hair abnormalities, and increased bone density in the cranium. The molecular mechanisms that explain the phenotypic characteristics of TDO syndrome have not been clearly determined. In this study, we examined phenotypic characteristics of wild type DLX3(wtDlx3) and 4-BP DEL DLX3 (TDO mtDlx3) in C2C12 cells. To investigate how wtDlx3 and TDO mtDlx3 differentially regulate osteoblastic differentiation, reporter assays were performed by using luciferase reporters containing the promoters of alkaline phosphatase, bone sialoprotein or osteocalcin. Both wtDlx3 and TDO mtDlx3 enhanced significantly all the reporter activities but the effect of mtDlx3 was much weaker than that of wtDlx3. In spite of these differences in reporter activity, electrophoretic mobility shift assay showed that both wtDlx3 and TDO mtDlx3 formed similar amounts of DNA binding complexes with Dlx3 binding consensus sequence or with ALP promoter oligonucleotide bearing the Dlx3 binding core sequence. TDO mtDlx3 exhibits a longer half-life than wtDlx3 and it corresponds to PESTfind analysis result showing that potential PEST sequence was missed in carboxy terminal of TDO mtDlx3. In addition, co-immunoprecipitation demonstrated that TDO mtDlx3 binds to Msx2 more strongly than wtDlx3. Taken together, though TDO mtDlx3 acted as a weaker transcriptional activator than wtDlx3 in osteoblastic cells, there is possibility that during in vivo osteoblast differentiation TDO mtDlx3 may antagonize transcriptional repressor activity of Msx2 more effectively and for longer period than wtDlx3, resulting in enhancement of osteoblast differentiation.

• Journal of the Korean Geographical Society
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• v.45 no.4
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• pp.431-443
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• 2010

The morphometric characteristics of 13 alluvial fans in the southern parts of the Korean Peninsula are studied and relationships between their distributions and causes are discussed by comparing them with 690 alluvial fans in Japan, Taiwan and the Philippines. The relationships between the surface gradient of alluvial fans and source basin area, the surface gradient and the relief ratio of the source basin, and the source basin area and the relief ratio were investigated. The alluvial fans in South Korea have typical characteristics of the Asian fans, although their frequency and density are relatively low, and the size and the relief ratio of their source basins are relatively small and steep, respectively. Moreover 36 major Korean river basins without alluvial fans tend to have lower altitude and relief ratios. Contrary to the argument by Blair and McPherson, the natural depositional gaps on the gradient of alluvial fans were not found. The Gyeongju alluvial fan is probably a fluvial fan with gentle gradient, whereas the other Korean fans seem to be debris-flow related on the base of their gradients.

• Korean Journal of Environmental Agriculture
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• v.22 no.4
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• pp.266-272
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• 2003

This study was conducted to evaluate effects of 2 constructed wetlands with different vegetation states (plumpton park wetland and Woodcroft park wetland) for reducing non-point source pollution from small watershed consisted of residential and agricultural area in suburban district of Sydney, Australia. The total nitrogen and phosphate removal efficiency of Plumpton park constructed wetland, composed of stable and dense vegetation, were 38.3% and 26.2% and Woodcroft park constructed wetland having still poor vegetation due to the short time to settle down transplanted plants after construction, showed relatively low removal efficiency of 20.2% and 14.0%. The removal efficiency of inorganic nutrients such as $NH_4-N$, $NO_3-N$, $PO_4^{-3}$ were higher than total nitrogen and phosphate because plants and microorganisms in rhizosphere of constructed wetlands took up inorganic nutrients shortly. According to the type of wetland inflow, the nutrients removal efficiency of storm water flow was lower than base flow.

• Archives of Plastic Surgery
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• v.36 no.5
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• pp.531-537
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• 2009

Purpose: Alveolar bone grafting has become an essential process in the treatmemt of alveolar cleft patient for stabilization of the maxillary arch, elimination of oronasal fistula, the reconstruction of the soft tissue nasal base support, and creation of bony support for tooth eruption for implant. The use of Autologous iliac cancellous bone is preferable because of the adequate quantity and high osteoinductive potential. However, even with iliac bone, insufficient osteoregeneration and absorption occur due to several factors such as the patient's age, cleft width, functional stress, and others. In order to increase osteoregeneration where the iliac bone is placed, the present study is associated with bone marrow aspirate (BMA). The experimental study evaluated the efficacy of osteoregeneration in normal cleft rabbits when alveolar bone grafting was performed with autologous iliac corticocancellous bone. Methods: Twenty - four New Zealand White rabbits were divided randomly into 2 groups (BMA, control). All animals underwent harvesting of corticocancellous bone graft from the right posterior iliac crest via standard surgical technique. $1m{\ell}$ of BMA were obtained by scraping the needle and aspirate with $10m{\ell}$ syringe from the contralateral iliac bone wall. The muco - periosteal flap on the palate was elevated. A mixture of Equal bone's volumes with BMA and saline as its control was inserted into the cleft. Animals were sacrificed at 2, 4, and 8 weeks and maxilla was harvested for dental peri - apical X-ray, bone matrix density (BMD),and histologic analysis. Result: BMD of regenerated bone to the cleft in the rabbits was higher than that of the control rabbits. X-ray, histologic analysis showed that increased osteoregeneration and low absorption rate were observed in the BMA group. Conclusion: Our experimental study showed BMA enhanced the osteoregeneration and survival rate of alveolar bone grafting. BMA is easy to extract & cost - time effective. So it can be an effective enhancers for bone grafting mixtures.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.1
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• pp.40-46
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• 2000

The investigation and the analysis of soil chemical properties, microbial flora and cultural practices in plastic house of 60 farms (pepper 20, cucumber 20, oriental melon 20) were conducted in Chonnam area film. An amount of fertilizer application of farms was more than that of standard fertilizer application. That was phosphate at two and a half times, potassium at twice and manure at three times the amount of standard application. It was inferred that excessive fertilizer application would lead to salts accumulation in soil. Continuous cropping injury would be incurred by excesive salts accumulation in soil. Even if inorganic elements in soil culturing peppers, cucumbers and oriental melons were greater than optimum level, base-saturation degree of soil culturing peppers, cucumbers and oriental melons was 40:18:12, 55:16:14 and 40:27:11 respectively. That was lower than the optimum levels (60:15:5). Therefore, crops would be affected by uptake inhibition by nutrition imbalance. Density of Fusarium is related to plant decease in soil i.e. pepper $26.2{\times}10^2\;cfu\;g^{-1}$, cucumber $75.8{\times}10^2\;cfu\;g^{-1}$ and oriental melon $6.5{\times}10^2\;cfu\;g^{-1}$. B/F of soil planted with pepper, cucumber and oriental melon was 91.2, 80.4 and 18.8, respectively.

• Journal of Internet Computing and Services
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• v.17 no.6
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• pp.17-23
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• 2016

In the future network such as Internet of Things (IoT), the number of computing devices are expected to grow exponentially, and each of the things communicates with the others and acquires information by itself. Due to the growing interest in IoT applications, the broadcasting in Opportunistic ad-hoc networks such as Machine-to-Machine (M2M) is very important transmission strategy which allows fast data dissemination. In distributed networks for IoT, the energy efficiency of the nodes is a key factor in the network performance. In this paper, we propose a fuzzy logic based probabilistic multi-hop broadcast (FPMCAST) algorithm which statistically disseminates data accordingly to the remaining energy rate, the replication density rate of sending node, and the distance rate between sending and receiving nodes. In proposed FPMCAST, the inference engine is based the fuzzy rule base which is consists of 27 if-then rules. It maps input and output parameters to membership functions of input and output. The output of fuzzy system defines the fuzzy sets for rebroadcasting probability, and defuzzification is used to extract a numeric result from the fuzzy set. Here Center of Gravity (COG) method is used to defuzzify the fuzzy set. Then, the performance of FPMCAST is evaluated through a simulation study. From the simulation, we demonstrate that the proposed FPMCAST algorithm significantly outperforms flooding and gossiping algorithms. Specially, the FPMCAST algorithm has longer network lifetime because the residual energy of each node consumes evenly.

• Journal of Ocean Engineering and Technology
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• v.30 no.4
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• pp.334-340
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• 2016

The combustion chamber of a diesel engine is often exposed to a more serious wear and corrosion environment than other parts of the engine because its temperature increases as a result of using heavy oil of low quality. Therefore, repair and built-up welding methods must be performed on worn or corroded parts of the piston crown, exhaust valve, etc. from an economical point of view. In this study, Inconel 718 filler metal was used in repair welding on the groove of a forged steel specimen for a piston crown, along with built-up welding on the surface of another forged steel specimen. Then, the corrosion characteristics of the weld metal zone for the repair welding and the deposited metal zone for the built-up welding were investigated using electrochemical methods in a 35% H₂SO₄ solution. The deposited metal zone indicated better corrosion resistance than the weld metal zone, showing a nobler corrosion potential, higher impedance, and smaller corrosion current density. It is considered that metal elements with good corrosion resistance were generally included in the filler metal, and these elements were also greatly involved in the deposited meta by built-up welding, whereas the weld metal consisted of metal elements mixed with both the filler metal and base metal elements because of the molten pool produced by the repair welding. Finally, it is considered that the hardness of the weld metal was increased by the repair welding, whereas the built-up welding improved the corrosion resistance of the deposited metal.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.477-484
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• 2019

Several types of scavenger receptors, including the Collectin-Placenta 1 (CL-P1) receptorthat is present in mammals, are molecules that are expressed on the surfaces of endothelial cells, macrophages and smooth muscle cells. These molecules are cell surface glycoproteins that can be conjugated to oxidized low density lipoprotein (oxLDL). Among these molecules, the effect of quercetin on CL-P1 activation has been confirmed. Quercetin is known as an antioxidant that stops oxidation because it acts to remove free radicals that are responsible for the oxidation reaction. In this study, fragments from the transcription start site of the mouse CL-P1 gene promoter to the -500th base were cloned using DNA polymerase. These fragments were then introduced into macrophage like RAW264.7 cells and fibroblast-like NIH3T3 cells to study the effect of quercetin on the CL-P1 gene expression. As a result, we found that bases ranging from -250 to -350 in the anterior part where gene expression starts are important for producing CL-P1 protein. Among them, the DNA mutation experiments we performed confirmed that the E2F binding sites are critical for producing the CL-P1 protein? In addition, when quercetin was added to the RAW264.7 culture medium, which was a culture of adherent cells, observedthe phenomenon of the cells falling off from the surface of the culture container.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.6
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• pp.859-865
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• 2018

A construction of infrastructures and base station on the moon could be undertaken by linking with the regions where construction materials and energy could be supplied on site. It is necessary to detect craters on the lunar surface and gather their topological information in advance, which forms permanent shaded regions (PSR) in which rich ice deposits might be available. In this study, an effective method for automatic detection of lunar craters on the moon surface is taken into consideration by employing a latest version of deep-learning algorithm. A training of a deep-learning algorithm is performed by involving the still images of 90000 taken from the LRO orbiter on operation by NASA and the label data involving position and size of partly craters shown in each image. the Faster RCNN algorithm, which is a latest version of deep-learning algorithms, is applied for a deep-learning training. The trained deep-learning code was used for automatic detection of craters which had not been trained. As results, it is shown that a lot of erroneous information for crater's positions and sizes labelled by NASA has been automatically revised and many other craters not labelled has been detected. Therefore, it could be possible to automatically produce regional maps of crater density and topological information on the moon which could be changed through time and should be highly valuable in engineering consideration for lunar construction.

• Journal of the Korean Society of Radiology
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• v.13 no.2
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• pp.185-192
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• 2019

DXA is the most commonly used BMD examination equipment with the best performance on reflecting the biological alteration with tiny change of bone density. In spite of the importance of the quality control to maintain the accuracy and precision of the examination, considerable number of hospitals are not conducting QC due to the difficulty and high cost of the phantom product. This study develops the cross revision phantom with 3D printer and the change of the degree of infilling filaments which can be readily secured, and provides the usefulness assessment of the developed phantom by comparing with existing products. The Hounsfield Units of ABS, TPU, PLA, 30% Cu-PLA, and 30% Al-PLA are assessed. The Hounsfield Units result at infilling rate 100% was $-149.74{\pm}2.36$, $-55.62{\pm}7.14$, $-7.68{\pm}3.82$, $87.53{\pm}1.07$, and $1795.20{\pm}16.15$. The L1, L2, L3 BMD of 3D printing phantom with linear regression model were $0.620{\pm}0.010g/cm^2$, $1.092{\pm}0.025g/cm^2$, $1.554{\pm}0.026g/cm^2$ which are statistically relevant to the existing phantom products. This result provides the base line data for various medical phantom produce and capability of proper quality control of DXA equipment.