• 한국환경농학회지
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• 제40권1호
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• pp.1-12
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• 2021

BACKGROUND: G protein-coupled receptors (GPCRs) are widely distributed in various organisms. Insect GPCRs shown as in vertebrate GPCRs are membrane receptors that coordinate or involve in various physiological processes such as learning/memory, development, locomotion, circadian rhythm, reproduction, etc. This study aimed to identify GPCRs expressed in fat body and compare the expression pattern of GPCRs in different temperature conditions. METHODS AND RESULTS: To identify GPCRs genes and compare their expression in different temperature conditions, total RNAs of fat body in Plutella xylostella larva were extracted and the transcriptomes have been analyzed via next generation sequencing method. From the fat body transcriptomes, genes that belong to GPCR Family A, B, and F were identified such as opsin, gonadotropin-releasing hormone receptor, neuropeptide F (NPF) receptor, muthuselah (Mth), diuretic hormone receptor, frizzled, etc. Under low temperature, expressions of GPCRs such as C-C chemokine receptor (CCR), opsin, prolactin-releasing peptide receptor, substance K receptor, Mth-like receptor, diuretic hormone receptor, frizzled and stan were higher than those at 25℃. They are involved in immunity, feeding, movement, odorant recognition, diuresis, and development. In contrast to the control (25℃), at high temperature GPCRs including CCR, gonadotropin-releasing hormone receptor, moody, NPF receptor, neuropeptide B1 receptor, frizzled and stan revealed higher expression whose biological functions are related to immunity, blood-brain barrier formation, feeding, learning, and reproduction. CONCLUSION: Transcriptome of fat body can provide understanding the pools of GPCRs. Identifications of fat body GPCRs may contribute to develop new targets for the control of insect pests.

• Journal of Radiation Protection and Research
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• 제45권4호
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• pp.163-170
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• 2020

Background: Gut microflora contributes to the nutritional metabolism of the host and to strengthen its immune system. However, if the intestinal barrier function of the living body is destroyed by radiation exposure, the intestinal bacteria harm the health of the host and cause sepsis. Therefore, this study aims to trace short-term radiation-induced changes in the mouse gut microflora-dominant bacterial genus, and analyze the degree of intestinal epithelial damage. Materials and Methods: Mice were irradiated with 0, 2, 4, 8 Gy X-rays, and the gut microflora and intestinal epithelial changes were analyzed 72 hours later. Five representative genera of Actinobacteria, Firmicutes, and Bacteroidetes were analyzed in fecal samples, and the intestine was pathologically analyzed by Hematoxylin-Eosin and Alcian blue staining. In addition, DNA fragmentation was evaluated by the TdT-mediated dUTP nick-end labeling (TUNEL) assay. Results and Discussion: The small intestine showed shortened villi and reduced number of goblet cells upon 8 Gy irradiation. The large intestine epithelium showed no significant morphological changes, but the number of goblet cells were reduced in a radiation dose-dependent manner. Moreover, the small intestinal epithelium of 8 Gy-irradiated mice showed significant DNA damaged, whereas the large intestine epithelium was damaged in a dose-dependent manner. Overall, the large intestine epithelium showed less recovery potential upon radiation exposure than the small intestinal epithelium. Analysis of the intestinal flora revealed fluctuations in lactic acid bacteria excretion after irradiation regardless of the morphological changes of intestinal epithelium. Altogether, it became clear that radiation exposure could cause an immediate change of their excretion. Conclusion: This study revealed changes in the intestinal epithelium and intestinal microbiota that may pave the way for the identification of novel biomarkers of radiation-induced gastrointestinal disorders and develop new therapeutic strategies to treat patients with acute radiation syndrome.

• 대한토목학회논문집
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• 제28권4B호
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• pp.421-428
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• 2008

최근 들어 우리나라 전해역에 걸쳐 발생하고 있는 침식은 사회적인 문제가 되어왔다. 하지만, 해안구조물에 의한 기존의 방법으로 해안선을 보호하는 것이 "파랑장에 친화적인가?"라는 관점에서 개선의 여지가 있다. 새로운 형식의 철재형 이안제는 권혁민 등(2007)에 의해 제안되었다. 본 형식은 다수의 파일과 상부에 파랑진행방향과 수평으로 설치된 소파장치로 구성되어 있다. 본 형식은 해수유통원활, 대수심 축조수월, 공기단축 등의 장점이 있다. 본 연구는 소파제로서 철재형 이안제의 상부에 거치가 가능한 이열 조합판의 투과율을 실험적으로 평가했다. 일방향 불규칙파 시스템에 의한 모형실험을 통하여 반사율, 소파율, 투과율에 관하여 분석했다. 실험결과, 파형경사가 클수록 그리고 조합판의 폭-파장비가 클수록 투과율이 작아짐을 보였다. 본 연구는 이열 소파판 조합에 대하여 투과율 산정을 위한 직선식을 제안했다.

• 한국응용과학기술학회지
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• 제38권6호
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• pp.1383-1392
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• 2021

호장근, 적하수오, 대황, 알로에 등과 같은 생약재의 주요 약리 활성 성분인 emodin은 항산화, 항균, 항염, 항암, 간보호 등에 효능이 있는 것으로 보고되었다. 본 연구에서는 emodin의 피부 질환 및 기능성 소재로서의 활용 가능성을 알아보기 위해 염증 개선과 피부장벽기능 개선 관련 활성을 확인하였다. human keratinocyte인 HaCaT 세포에 대하여 항염효과를 관찰하기 위해 cytokine억제능은 ELISA kit로, 단백질 발현은 western blot으로 확인하였다. TNF-α (10 ng/mL)/IFN-γ (10 ng/mL)로 활성화된 HaCaT 세포에서 emodin을 농도별(5, 10, 20, 40) µM로 처리한 결과 TNF-α, IL-1β 및 IL-6의 생성량은 emodin의 농도가 증가함에 따라 감소됨을 확인하였다. 염증관련 단백질인 iNOS, COX-2 발현량에 대한 실험결과에서도 emodin 20 µM 농도에서 control 대비 iNOS는 48%, COX-2는 29%가 저해됨을 확인하였다. 피부장벽기능 개선의 지표로써 filaggrin, involucrin, loricirn의 mRNA 발현 정도와 filaggrin, involucrin, loricirn의 생성량을 확인한 결과 에모딘 농도에 의존적으로 증가하는 우수한 결과가 얻어졌다. 특히 20 µM 농도에서 대조군 대비 2배의 생성량 증가를 보인 filaggrin은 천연보습인자인 NMF의 형성에 관계되는 단백질로 각질층의 보습에 중요한 역할을 하는 것으로 알려져 있다. 결론적으로 emodin의 피부 질환 및 기능성 소재로서의 활용 가능성 중 염증 개선 및 피부장벽기능 개선 소재로 활용될 수 있음을 확인하였으며 향후 추가적인 연구가 수행되면 그 활용 범위가 더 넓어질 수 있을 것으로 사료된다.

• 한국지반신소재학회논문집
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• 제5권3호
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• pp.31-36
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• 2006

본 연구에서는 보호, 여과, 차수기능을 갖는 부직포 지오텍스타일과 지오멤브레인의 응력완화거동을 각각 고찰하였다. "두 가지 지오신세틱스 제품의 응력완화거동을 해석하는데 "전이현상이론"이 적용되었다. 이들 지오신세틱스 제품의 응력완화거동에서 초기 및 후기 완화시간은 구성방정식으로부터 구하였다. 이들 지오신세틱스 제품의 초기완화거동은 부가변형률에 대한 의존성이 있으며, 특히 온도가 증가 할수록 증가하였다. 끝으로, 이들 지오신세틱스 제품의 초기 및 후기 완화시간은 부가변형률 및 온도가 커질수록 짧아졌으며, 완화시간 감소는 지오멤브레인보다 부직포 지오텍스타일의 경우가 더 큼을 알 수 있었다.

• 무역상무연구
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• 제76권
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• pp.103-123
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• 2017

If additional condition in letter of credit is used in malicious way, it affects the international trade transaction in jeopardy. Therefore, it's significant to identify whether additional conditions are malicious or ordinary in the transaction with letter of credit. In normal cases, thanks to lots of useful features as an international payment method, such as security of payment, legal protection, and versatility, a letter of credit is widely used in international trade. However, even with these advantageous features, a letter of credit is complicate and costly to use, compared to other payment methods. Furthermore, due to its principle of independence from underlying contract, a use of letter of credit creates another type of concern for proper handling and needs significant caution upon field use. At some points, malicious additional conditions are used for buyer's advantage in deal making and fraud instance in worst situation. In addition, some countries request malicious conditions against sellers as a non-tariff barrier. Therefore it's extremely important to recognize whether malicious additional condition exists in letter of credit and, if so, how to deal with it. This study delivers the information to distinguish and categorize the malicious conditions in various cases and to figure out how to deal with them for safer trade with less risk.

• The Plant Pathology Journal
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• 제20권4호
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• pp.277-282
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• 2004

Amendments of a recirculating nutrient solution with potassium silicate were evaluated as a means to control Phytophthora capsici infections on pepper plant(Capsicum annuum L.). Supplying the solutions with 100 or 200 ppm of silicate significantly reduced motility, root decay, and yield losses attributed to infection of P. capsici. Treating inoculated plants with potassium silicate increased root dry weights and number of fruit, especially high-grade fruit. Results were slightly superior to non-inoculated controls. The two varieties, PBC 137 and PBC 602, responded similarly to the treatments. No significant differences were observed between the 100- and 200 ppm silicate treatments. Results were better when greenhouse conditions favored the spread of P. capsici. Silicon alone did not increase pepper yield, suggesting that it acts as a disease suppression agent rather than as a fertilizer, The phenomena by which silicon confers protection against P. capsici infection and disease development are not fully understood, but our results indicate that mechanisms other than a mechanical barrier to fungal penetration are involved.

• Journal of Applied Biological Chemistry
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• 제50권2호
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• pp.41-45
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• 2007

Polaromonas naphthalenivorans CJ2, responsible for naphthalene degradation at a coal tar contaminated site, was isolated on MSB agar media supplied with naphthalene vapor as the sole carbon source at $10^{\circ}C$. The strain is not isolated under the same isolation condition using the same soil sediment at $20^{\circ}C$ although its optimum temperature is about $20^{\circ}C$. In this work we explored the reason why strain CJ2 could not have been isolated on MSB agar with naphthalene vapor at $20^{\circ}C$. Dispersed CJ2 cells in PBS buffer formed colonies on MSB agar with naphthalene vapor at $10^{\circ}C$ with low naphthalene vapor pressure, but not at $20^{\circ}C$ with high naphthalene vapor pressure. However, streaked cells without resuspension grew on MSB agar with naphthalene vapor at $10^{\circ}C,\;20^{\circ}C$, and even $25^{\circ}C$. Investigation of scanning electron microscopy showed that CJ2 cells formed extracellular polysaccharide (EPS) capsules, which were released easily from CJ2 cells by just dispersion. Therefore, it is concluded that strain CJ2 is able to overcome the naphthalene toxicity by forming a capsule-type barrier around the cells although it is susceptible to naphthalene toxicity at high temperature.

• 한국경제지리학회지
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• 제7권2호
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• pp.137-156
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• 2004

본 논문은 우리나라의 왜곡된 정치 현실과 경제적 상황을 가장 극명하게 나타내는 경기도 접경지역의 실태를 고찰하여 이 지역에 대한 종합적 지리학 연구의 기초 자료를 제공하는데 목적이 있다. 연구 결과 경기도 접경지역은 첫째, 군사시설보호법에 의한 규제로 주민들의 생활과 경제적 활동이 제한되어온 지역임을 알 수 있었다. 둘째, 수도권 과잉집중을 해결하기 위해 시행된 수도권정비계획법은 수도권 북부지역 및 접경지역의 개발에 또 하나의 제약조건이 되고 있다. 셋째, 경제관련 지표 분석 결과 경기접경지역은 이러한 규제들로 인해 낙후성을 면치 못하고 있음을 알 수 있었다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.II
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• pp.1276-1279
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• 2005

The long-term stability of pentacene thin-film transistors (TFTs) encapsulated with a transparent $SnO_2$ thin-film prepared by ion beam assisted deposition (IBAD) was investigated. With a buffer layer of thermally evaporated 100 nm $SnO_2$ film deposited prior to IBAD process, our encapsulated OTFTs sustained its initial field-effect mobility up to one month and then gradually degraded showing only 37% reduction compared to 90% reduction of non-encapsulated OTFTs after 100 days in air ambient. The encapsulated OTFTs also exhibited superior on/off current ratio of over $10^5$ to that of the unprotected devices $({\sim}10^4)$ which was reduced from ${\sim}10^6$ before aging. Therefore, the enhanced long-term stability of our encapsulated OTFTs should be attributed to well protection of permeation of $H_2O$ and $O_2$ into the devices by the IBAD $SnO_2$ thin-film which could be used as an effective inorganic gas barrier for transparent organic electronic devices.