• Title/Summary/Keyword: Barley Seed

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Effects of Dimethipin on α-amylase Activity of Barley Seeds (보리 종자의 α-아밀라아제 활성에 미치는 Dimethipin의 영향)

  • Lee, Joon-Sang
    • Journal of Environmental Science International
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    • v.16 no.4
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    • pp.409-414
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    • 2007
  • Effects of dimethipin on ${\alpha}$-amylase activity of barley seeds were investigated. In the treatments of $1{\mu}M\;and\;10{\mu}M$ dimethipin, the indexes of germination were reduced to 17% and 24 % respectively. After seed germination, dimethipin was added to germinated seedlings and then the seedlings were kept to measure seedling length under illumination for 7 days. In control, the length of seedling was 5.7 cm, but in the treatments of $1{\mu}M$ dimethipin and $10{\mu}M$ dimethipin, seedling lengths were 5.5 cm and 1.2 cm respectively. In the relationship between dimethipin concentrations and ${\alpha}$-amylase activities, there was a linear curve. The more dimethipin was added to the seeds, the more ${\alpha}$-amylase activities were inhibited. In the treatments of $1{\mu}M$ dimethipin and $10{\mu}M$ dimethipin, ${\alpha}$-amylase activities were reduced to 33% and 71% respectively. Dimethipin also inhibited ${\alpha}$-amylase activities increased by gibberellin and the content of soluble protein. Therefore, it could be suggested that dimethipin might inhibit directly the activities of hydrolysis enzymes including ${\alpha}$-amylase or the expression of ${\alpha}$-amylase genes as germination and seedling growth were severely disturbed.

Phytotoxic Effect of Xanthium occidentale Leaf Extract on Seed Germination and Early Seedling Growth of Alfalfa and Barnyard Grass

  • Chon, Sang-Uk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.1
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    • pp.30-35
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    • 2004
  • Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous extracts from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine their allelopathic effects, and the result showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Untreated seeds germinated in 60h, but extract concentrations greater than 30g $\textrm{L}^{-1}$ delayed seed germination. The extracts significantly inhibited seed germination of alfalfa, and $\beta$-amylase activity of alfalfa and barley seeds during 24-36 hours after treatment. Aqueous extracts of 40 g $\textrm{L}^{-1}$ from X. occidentale were completely inhibited the hypocotyl and root growth of alfalfa. Aqueous leaf extracts showed the highest inhibitory effect and followed by root and stem extracts. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol extracts. By means of high-performance liquid chromatography, chlorogenic acid and trans-cinnamic acid were quantified as the highest amounts from water and EtOAc fractions, respectively. BuOH and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did hexane and water fractions. The findings of the bioassays for aqueous or methanol extracts reflected that the inhibitory effect of extract was closely related to the level of responsible allelochemicals found in plant extracts.

Nutritional Evaluation of Imported Organic Feeds and Locally Produced Agricultural By-products for Organic Ruminant Farming (유기 반추동물 전용 수입산 유기사료 및 국내산 유기 부산물의 사료적 가치 평가)

  • Park, Joong-Kook;Kim, Chang-Hyun
    • Korean Journal of Organic Agriculture
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    • v.19 no.4
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    • pp.513-528
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    • 2011
  • This study was conducted to evaluate the nutritional value of locally produced organic agricultural by-products to substitute imported organic feeds for organic ruminant farming. Imported organic feeds (corn grain, soybean meal, soybean seed, oat grain, barley grain, wheat grain, buckwheat, sunflower seed meal) and byproducts (rice bran, grape seed meal, rice straw, soybean hull, soybean curd, rice hull, green kernel rice, and crushed rice grain) were analyzed for chemical composition and NDF, ADF, mineral, and amino acid contents and anti-nutritional factors. Dry matter, NDF and ADF contents in organic feeds were higher than those in conventional feeds. Especially, the 9.65% fat content of organic soybean meal was 6 times higher than the 1.95% fat content of conventional soybean meal. Fat contents of rice bran, grape seed meal, green kernel rice, and crushed rice grain were 25.66, 6.09, 3.57 and 1.59%, respectively. Protein contents of soybean hull and soybean curd were 14.68 and 19.87%, respectively, which are highest among organic by-products. Levels of aflatoxin in all feeds were below the safety level. Therefore, organic rice bran, green kernel rice and crushed rice as energy source, and soybean hull and soybean curd as protein source could partial replace imported feeds for organic ruminant farming.

Changes of Chloroplast-Mediated Electron Transport Activity and Chlorophyll-Protein Complexes in Barley Seedlings by Decursinol (Decursinol 처리에 따른 보리 유식물의 전자전달 활성과 엽록소-단백질 복합체의 변화에 대하여)

  • 이현식
    • Journal of Plant Biology
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    • v.31 no.2
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    • pp.131-141
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    • 1988
  • The effects of decursinol and decursin on chloroplast-mediated electron transport and phosphorylation in barley seedlings were investigated in comparison with coumarin in the dark or light. The changes of CP-complexes were also studied. Decursinol, decursin and coumarin caused marked inhibitory effects on germination of seed and electron transport and phosphorylation activity of seedlings. The following order of inhibitory effectiveness was exhibited; decursinol>coumarin>decursin. Loss of chlorophyll and decrease of electron transport activity were retarded in the dark, but were reversely accelerated in the light by these three chemicals. The changes of CP-complex patterns were also similar to effects on chlorophyll content and the electron transport activity. These opposite effect in the dark and light suggest that these three chemicals act as natural growth retardants rather than cytokinins or growth inhibitors.

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Relationship between Yield and Weather Elements of Barley in Sunchon Area, Korea

  • Kwon, Byung-Sun
    • Plant Resources
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    • v.7 no.1
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    • pp.54-59
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    • 2004
  • This study was conducted to investigate the relationships between yearly variations of weather elements and productivity in rice. In addition, coefficients of correlation among yield and yield components were used to find out the relationships between weather elements and productivity. Coefficients of variance (C.V.) of air temperature mean was considerable with 25%, but the variation by duration of sunshine was small in May. Culm length and number of spikes were great with c.v. of 21.5, 16.4%, respectively. Coefficients of correlation between temperatures of cultivation in May and yield were positive correlations. Coefficients of correlation between precipitation and sunshine of cultivation period from Oct. to May and yield were negative correlations. Coefficients of correlation amount the culm length, number of spikes, 1,000 grains wt. of seed, and yield were positively significant at the level of 1 %, respectively.

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The Study on Temperature Measurement for the Standardization of Moxibustion (구법(灸法)의 표준화를 위한 온도 측정 연구(硏究))

  • Kim, Yoon-Hong;Lee, Seung-Ho;Yeo, Su-Jung;Choe, Il-Hwan;Kim, Young-Kon;Lim, Sabina
    • Journal of Acupuncture Research
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    • v.25 no.2
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    • pp.129-138
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    • 2008
  • Objectives : The moxibustion is the method in using the heat stimulation made of attached and burned a moxa or other herbal materials on a healing point or acupuncture point and the chemical stimulation of a resin made from burning them. We need to standardize the characteristics of moxa combustion in order to get more systematic and objective result in operation mechanism and effects and then get more clinical abilities in these fields. Methods : In this study, using of labview system on the moxibustion. 1. we studied relation the size of barley and jujube seed with the moxa cone's peak combustion temperature and then measured and compared the moxa cone's peak combustion temperature by tonification and sedation method. 2. we measured the peak combustion temperature of indirect moxibustion. Results & comclusions : 1. When we measured a direct moxibustion's combustion temperature, the jujube seed sized moxa cone's peak temperature was higher than the barley sized moxa cone's when it burned and the time to peak temperature of the barley sized moxa cone was shorter than the jujube seed sized moxa cone's. 2. When the direct moxibustion was burned by the tonification and sedation method, the sedation method's peak temperature was higher than the tonification method's, the tonification's time to peak temperature was shorter than the sedation method's. 3. When we measured the temperature of the moxa combustion in the kinds of sliced herbal materials, the peak temperature of indirect moxibustion with a ginger when it sliced a 1.3mm size and a 1.5mm size was degreed within $40^{\circ}C$ to $52^{\circ}C$ and the peak temperature of indirect moxibustion with a garlic when it sliced a 1.7mm size was degreed within $46^{\circ}C$ to $62^{\circ}C$.

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Protein of Major Germplasms for High-Protein Wheat Breeding (밀 주요 교배모본의 단백질 특성)

  • Ha, Yong-Woong;Song, Hyeon-Suk;Lee, Choon-Ki;Cho, Chang-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.5
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    • pp.393-402
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    • 1990
  • Studies to obtain a fundamental data to improve the quantity and quality of wheat protein and to select the excellent breeding materials suitable for various uses of it were performed on the focuses of protein content, amino acid composition, and its related quality. The range of seed protein content of crossing block consisted with 319 varieties or lines was from 9.9% to 17.6% with the average of 13.2%. The minimum protein content was showed by Wonkwang, and the maximum by Chungnamjaerae and Suwon 210. Among the Korean native varieties or breeding lines of crossing block, at least twelve lines or varieties were selected as the high-protein wheats. In the effects of environmental conditions, the variations of protein content by cultivating locations and year were showed out to be up to 3.6%, and 3.3%, respectively. But the varietal characteristics of wheat protein were seemed to remain constantly. In the results of amino acid analysis, those related with bread quality, except proline were improved with the increase of seed protein. But the improvement of nutritional quality did not agree with the protein content increase of seed. The total content of amino acid essential to adults was very low with 266.6-273.3mg per one gram of protein compare to the 350mg (free from the amino acid of tryptophan) recommended by FAO/WHO. Especially the limiting amino acid such as methionine, lysine and threonine were critically deficient. The correlation between protein contents and sedimentation values was obtained r=0.656$\^$**/ of average notwithstanding widly different environmental conditions, and in some condition the value was obained about to r=0.956$\^$**/.

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Induction of Male Sterility in Barley and Wheat with 2-Chloroethylphosphonic acid (Ethrel) (Ethrel에 의한 맥류의 웅성불임 유발)

  • Eung-Ryong Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.7-14
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    • 1972
  • Four treatment leveles(check 500, 1, 000 2, 000ppm) of Ethrel were applied to barley and wheat grown in greenhouse and fields at three stages (preboot, boot, afterboot) of growth in orderd to induce male sterility and to evaluate possibility of practical $F_1$-hybrid-seed production as gametocide. The results are summarized as follows. 1. The shortening plant height, especially the first internode length. spike length and decreasing 1, 000 seeds weight and induction of male sterility, were observed with all Ethrel theatments. The earier the stage of spraying at the boot and the higher concentration of Ethrel, the greater effects were observed. 2. The most practical level of inducing male sterility occurred ranging from 1, 000-2, 000ppm applied at all stages, while with increasing maturity, greater concentration of Ethrel were required to induce the same level of male sterility. .3. Great differences for male sterility per spike in bagged and unbagged spikes were shown with all treatments indicated ovary receptiveness, and that out-crossing has taken place, 4. Ethrel treatment induced more practicable extent of male sterility in barley than wheat. This results indicated that $F_1$-hybrid-seed production in barley with gametocide appears feasible if high combining ability lines were selected for increased out-crossing ratio. 5. There seemed to be no indication of additive surfactant influence on the Ehrel action in the present studies, and showed an interest on further studies on this matter.

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Spatiotemporal Patterns of Starch Deposition in Amaranth Grains (Amaranthus cruentus L.)

  • Young-Jun Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.173-173
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    • 2022
  • In this study, we investigated whether there is another amaranth GBSS isoform in an attempt to characterize the synthesis of amylose in the pericarp. We used I2/KI staining to analyze the temporal and spatial starch accumulation patterns during seed development. The spatiotemporal starch accumulation patterns in developing seeds were observed by staining with I2/KI. Starch granules were observed in the pericarp in the initial developmental stage (3 DAP). A few starch granules were detected in the perisperm in the early-late developmental stage (8 DAP), during which the pericarp starch contents rapidly decreased. Starch granules were distributed throughout the perisperm in the mid-late developmental stage (15 DAP). Similar results were reported for other cereal crops, including barley, rice, and sorghum. Starch granules in the pericarp are synthesized during the early seed developmental stages but are absent in mature seeds. We recently reported that starch deposits in the perisperm of developing amaranth seeds are detectable only after the initial developmental stage. Prior to this stage, the pericarp is the major site of starch deposition. A recent study suggested that GBSSII isoforms are responsible for amylose synthesis in pericarps.

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