• Title/Summary/Keyword: Banding

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Chromosome Analysis by GTG, High-Resolution, and NOR-banding Techniques in the Dog (Cams familaris) (GTG, High-Resolution, Nor-banding에 의한 개의 염색체 분석)

  • 김종봉;윤인숙
    • Journal of Life Science
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    • v.12 no.5
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    • pp.605-609
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    • 2002
  • None of the numerous published canine idiograms and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. To establish canine banded karyotype from the 22nd chromosome to the 37th chromosome, we analyzed canine chromosomes by GTG, high resolution, and NOR-banding techniques. The GTG and high resolution banding patterns of canine chromosomes corresponded to other reports described previously except for a few chromosomes. While other researchers observed 12 bands, we observed 7 bands in the banding patterns of chromosome 24, 34 and 37. On the other hand, the banding patterns by NOR-banding technique showed that three pairs of autosomes have nucleolus organizer regions at the terminal ends of their long arm, and the Y chromosome has it in its short arm terminal. However, the X chromosome has no nucleolus organizer like other mammals.

An Experimental Study of Percutaneously Adjustable Pulmonary Artery Banding Device (가변성 폐동맥 혈류조절기의 실험적 연구)

  • Gang, Gyeong-Hun;Kim, Sang-Hyeon
    • Journal of Chest Surgery
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    • v.30 no.6
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    • pp.580-584
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    • 1997
  • To overcome the problems of classical pulmonary banding procedure, we developed a percutaneously adjustable pulmonary artery ba ding device. The banding device consists of banding portion of zig-zag shaped self-expandable stainless steel wire, shaft portion made by a polyvinyl catheter and a screw adjuster which includes a bolt and a nut. As the screw moves, the diameter of banding portion changes. Four Mongrel dogs ranging from 15 kg to 20 kg in weight underwent the banding of the mid portion of descending aorta with this devices through the left thoracotomy. One month after operation, we evaluated the pressure changes by controling the banding with the devices and then the dogs were sacrificed to study the microscopic changes in the aorta. The diameter and circumference of the band could be easily and finely adjustable by the screw control. The pressure recordings . revealed a linear increase and decrease in pressure gradient according to percutaneous ad;ustment of the banding device. Since the banding device can be easily placed and simply adjusted percutaneously, we hope the banding device may be applicab e clinically to improving the safety of pulmonary artery banding and debanding procedures in the future.

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Adjustable Pulmonary Artery Banding Device (가변형 폐동맥 협착기구의 개발)

  • 김해균;이두연;김동관;이교준;박재희;구경모
    • Journal of Chest Surgery
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    • v.26 no.2
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    • pp.71-74
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    • 1993
  • For Percutaneous control of the degree of constriction after pulmonary artery banding, we developed an adjustable banding device that was operated by oil pressure. This consists of a stainless steel snare, a polyethylene tube with silicone oil, and a screw adjuster. Five dogs underwent banding of the pulmonary artery or the descending aorta with this device. This band could be effectively and finely adjusted. Although these studies are preliminary, they suggest that a reversible pulmonary artery band can be performed.

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A comparative Karyotype study on Korean Squirrels. II. Karyotype Analysis of Sciurus vulgaris coreae and Tamias sibiricus asiaticus by G-banding Method. (한국산 다람쥐 핵형의 비교연구 II. G-banding 방법에 의한 한국산 청서(Sciurus vulgaris coreae)와 다람쥐(Tamias sibiricus asiaticus)의 핵형분석)

  • 김종봉;이희영
    • The Korean Journal of Zoology
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    • v.33 no.3
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    • pp.255-259
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    • 1990
  • The Karyotypes of Korean Sciurus uulgaris coreae and Tamias sibiricus asiaticus were analyzed by the G-banding method. Chromosomes of two species could be identified by G-banding patterns. The banding patterns of chromosomes 9, 10, 12 and X of S. vulgaris coreae were identical to those of chromosomes 6, 9, 12 and X, respectively of T. sibiricus asiaticus. It was shown that chromosomes 4, 10, 7 and 17 of T. sibiricus asiaticus resulted from pericentric inversion of chromosomes 1, 7, 8 and 16 of S. vulgaris coreae. These results suggested that pericentric inversion was an important factor in the karyological differentiation of two species.

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Identification of Chromosomal Band Marker of Korean Native Chick by High-resolution Banding Technique (고분염분석법(High-resolution banding)에 의한 한국재래계의 염색체 분염 표지 설정)

  • 백규흠;손시환
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2000.11a
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    • pp.85-87
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    • 2000
  • To obtain the genetic information of Korean native livestock, the karyotyping of Korean native chick were performed by high-resolution banding technique. The chromosomes were prepared from lymphocyte culture and early embryos with 200 Korean native chick which have been raised at National Livestock Research Institute. There were no significant difference between Korean native chick and Leghorn in the number of chromosomes and chromosomal morphological pattern. Using high resolution banding technique, the yield of G-bands of prophase is much greater than that can be obtained by International System for Standardzed Avian Karyotypes(ISSAK, 1999). The G-band landmarks of Korean native chick were similar to those of ISSAK and Leghorn except some macrochromosomes. chromosome Z and 3 had C-band variants with heteromorphic patterns on distal and centromeric site. The proportion of constitutive heterochromatin, the heterochromatin ratio of Korean native chick was significantly more than that of Leghorn in all chromosomes.

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G-and C-Banding Pattern Analyses of Korean Rodents: I. Chromosome Banding Patterns of Striped Field Mice (Apodemus agrarius coreae) and Black Rats (R. rattus rufescens)

  • Koh, Hung-Sun
    • The Korean Journal of Zoology
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    • v.25 no.2
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    • pp.81-92
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    • 1982
  • G- and C-banding pattern analyses of striped field mice (Apodemus agrarius coreae) using 17 specimens from four localities in Korea revealed that centromeric heterochromatin results in the variation of No. 1 chromosome pair (telocentri $c_telocentric), i.e., centromeric heterochromatin sometimes appeared to be recognized as short arm. G- and C-banding patterns of four black rats (R. rattus rufescens) from two localities in Korea showed that No. 1 chromosome polymorphism (telocentri $c_telocentric) is due to pericentric inversion. In addition, G- and C-banding patterns of black rats mentioned above are idiogrammed.ammed.

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Chromosome analysis by GTG banding technique in the DongGyeongi dogs (GTG banding에 의한 경주지방의 무미 또는 단미 형태의 개(경주개 동경이)의 핵형분석)

  • Choi, Seog-Kyu;Sung, Ki-Chang;Lee, Eun-Woo;Park, Chang-Eun
    • Korean Journal of Veterinary Service
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    • v.33 no.2
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    • pp.207-211
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    • 2010
  • None of the numerous published canis idiogram and karyotypes has yet been generally accepted as a standard one because the dog has 76 acrocentric autosomes of similar size and shape. The karyotypes of DongGyeongi dog were analysed by conventional trypsin/Giemsa staining (GTG-banding techniques), and were compared with one another. There were no variations in karyotypes which were analysed by conventional GTG-banding techniques, but differences were observed in G-banding patterns with sapsaree (or canis familiaris strains). It is not clear that these disagreements in G-banding patterns between strains of dog were caused by chromosome polymorphism or a difference in interpretation.

Methodology of Chromosome Preparation and Banding Analysis in Gallus domesticus (닭 염색체의 분리 분석 방법에 관한 연구)

  • 손시환;오봉국
    • Korean Journal of Poultry Science
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    • v.14 no.2
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    • pp.89-96
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    • 1987
  • The purpose of this paper to present morphological normal chick chromosomes and develope avian cytogenetic techniques including chromosome preparation and banding technique. The early chick embryos provide a consistent source of material with hish mitotic cells. Although chick embryo tissue gives excellent preparations, the 4-5 days embryo is somewhat incovenient materials, Most imp of ant for avian Chromosome analysis are the technical protocols to achieve adequate preservation, spreading, and staining of the full chromosome complement. To precise chromosome analysis, pro-metaphase states are required. Best results of banding will be obtained from air dried slides prepared from early chick embryos that have been aged at least 1 week. Good G-banding differentiation is achieved by adequate trypsin digestion fellowed by staining in Goe,sa dye. The results of C-banding is influenced by many factors including the conditions of Ba(OH)$_2$, HCl treatment, and state of rinsing. In addition to precisely interprets banding patterns, the densitometric analysis is recommended.

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Karyotyppes of the Korean birds II. Karyological analysis on four species of genus Pans by C-banding method. (한국산 조류의 핵형 II. C-banding 방법에 의한 박새속 4종의 핵형분석)

  • 이성근;이혜영
    • The Korean Journal of Zoology
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    • v.32 no.4
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    • pp.365-373
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    • 1989
  • 한국산 박새속 조류 Porus maior(박새), Parus ater(진박새), Parus palustris(쇠박새), Parus varius(곤줄박이)의 핵형을 C-banding 방법으로 분석한 결과 ZZ-ZW 염색체를 동정, 확인할 수 있었으며, 구조적 이질염색질의 부위가 일반염색 방법에 의한 핵형 비교에서 보다 종간에 더 많은 차이를 나타냈다. 그러나 일반염색 방법과 C-banding 방법 만으로는 4종간의 종분화 기작을 추측할 수 없었다. The chromosomal analysis of Paws motor, Paws ater, Pahs palustris and Pows varius of the genus Paws in Korea were performed by C-handing method. The identification of sex chromosones was ascertained and the part of constitutive heterochromatin was more different between interspecies than by conventional Giemsa staining method. However, conventional Giemsa staining and C-banding methods could not explain mechanisms of speciation between four species.

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Studies on the Chromosomal Banding Analysis of Korean Native Fowl (한국재래계의 염색체 분양분석에 관한 연구)

  • 오희정;오봉국
    • Korean Journal of Poultry Science
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    • v.16 no.4
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    • pp.201-207
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    • 1989
  • This study was carried out to identify the chromosome morphological structure and G-, C-banding pattern of Korean native fowl. The samples used in this study were early chick embryos, and the method of chromosomal analysis quoted from the protocal of Ohio univ. with more or less modified. The results were summerized as follow as; 1. In each of macrochromosomal morphology, the arm-ratio, centromeric index, and relative length of Korean native fowl were more or less different from improved breeds, but the designations were the same. 2. The graphical pecks, by densitometric recordings, in each macrochromosome number of 1, 2, 3, 4, Z, and 5, numbered 21, 14, 12, 8, 11, and 4 in G-banded, and 16, 13, 9, 9, 9, and 4 in C-banded, respectively. Those pecks could be explained as a consequence of chromosome condensation during mitosis and of genetic material differences.

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