• Korean Journal of Pharmacognosy
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• v.30 no.2
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• pp.202-206
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• 1999

The MeOH extracts obtained from the leaves of Eucommia ulmoides were solvent-fractionated with EtOAc, n-BuOH, and $H_20$, successively. From the EtOAc extract showing Grab2-Shc inhibitory activity, a flavonol and a lignan compounds were isolated through repeated silica gel column chromatographies. By interpretation of several spectral data and adaptation of acetylation method, the chemical structures of the compounds were determined as 5,7,3',4'-tetrahydroxy-flavonol (quercetin), and 4,4',8',9-tetrahydroxy-3,3'-dimethoxy-7,9'-cyclolignan {(-)-olivil)}, respectively. The compounds exhibited $IC_{50}$ values in Grb2-Shc inhibitory activity to be 93 and $210\;{\mu}mole/l$, respectively.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1397-1401
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• 2005

Bone morphogenetic protein-4 (BMP-4) is a signaling homodimeric molecule that acts as a morphogen to influence cell fate in a concentration-dependent manner. The limited supply of a pure preparation of BMP-4, due to very low level of their expression in vivo, makes it difficult not only to study the biological activities of BMPs, but also to use them as a clinical tool. For a large-scale production of BMP-4, human BMP-4 cDNA was expressed in Chinese hamster ovary (CHO) cells by a recently development vector system, which confers position-independent stable expression of the foreign genes. The CHO cell line expressing recombinant human BMP-4 (rhBMP-4) at the level of $7\;{\mu}g/ml$ could be obtained after stepwise selection with methotrexate. This level of expression is about 70 times higher than those previously reported. The partially processed form of BMP-4 as well as mature form could be detected, when the aliquots of culture media were analyzed by Western blot. The glycosylation pattern and biological activity of the rhBMP-4 were determined by glycosidase treatment and the induction rate of alkaline phosphatase in mouse osteoblastic cells.

• Journal of Applied Biological Chemistry
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• v.57 no.1
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• pp.29-32
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• 2014

This study was initiated to search for the most effective method for extraction of anthocyanins from Morus alba Fruits using organic acids and to evaluate the stability of the pigments at various storage environments. The anthocyanins were effectively extracted by addition of organic acids such as citric acid, malic acid and fumaric acid. The anthocyanins were not degraded at $25^{\circ}C$ and under a fluorescent light with 183 lux, but sharply degraded at $60^{\circ}C$.

• Advances in Traditional Medicine
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• v.10 no.4
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• pp.271-277
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• 2010

We tested to determine if Mori Cortex Radicis extract has antioxidant activities and its potential mechanism of action was explored. Anti-oxidative effects were tested by measuring free radical and nitric Oxide (NO) scavenging activity, and reducing power. Since iNOS and COX-2 are important enzymes responsible for the production of free radicals in the cell, Mori Cortex Radicis extract was tested as to whether it could inhibit iNOS and COX-2 expression in LPS stimulated Raw cells. 70% methanolic extract of Mori Cortex Radicis exerted significant DPPH free radical and NO scavenging activities. In addition, the Mori Cortex Radicis extract exerted dramatic reducing power with maximal activity observed at 1 mg/ml (11-fold over control). Production of iNOS induced by LPS was significantly inhibited by the Mori Cortex Radicis extract, suggesting it could inhibit NO production by suppressing iNOS expression. COX-2 induced by LPS was also significantly inhibited by the Mori Cortex Radicis extract. The extract contains well known antioxidant components including phenolics, flavonoids and anthocyanin at the concentration of 0.23 mg/g, 42.97 mg/g and 12.08 mg/g, respectively. These results suggest that 70% methanolic extract of Mori Cortex Radicis exerts significant anti-oxidant activity via inhibiting iNOS and COX-2 induction.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.70.2-70.2
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• 2012

The night sky in Mongolia is known to be one of the darkest and clearest in Asia. The seasonal dust winds from the Gobi desert, however, might degrade the transparency of the night sky and affect the photometrical quality for astronomical research. In this poster, we present the preliminary results of the first transparency measurement of the night sky in Monglia. The observations are done at Zuunmod located at 25 km south from Ulaanbaatar in 2011 October 26-31. We used a CCD camera (QSI 583s) attached to an 80 mm aperture refractor telescope (William Optics Zentih Star 80 II ED APO). A new supernova, SN2011fe, and a young stellar object, HBC 722, were monitored in order to measure the extinction coefficients. These values in Mongolia are compared with those in other astronomical observatory.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.810-815
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• 2004

Many biological properties and the clinical potential of human interleukin-2 (hIL-2) draw much attention to its high-level expression in mammalian cells. Recombinant human IL-2 (rhIL-2) was expressed in Chinese hamster ovary (CHO) cells, using the recently developed expression system which confers position-independent expression. Stable CHO cell lines carrying several hundred amplified copies of the rhIL-2 gene were easily obtained and rhIL-2 was expressed at high levels after selection with increasing concentrations of methotrexate. Interestingly, the insertion of the transcription terminator of the human gastrin gene into the downstream region of the gene for rhIL-2 considerably increased rhIL-2 expression. Using the expression system with the transcription terminator, it was possible to get a CHO cell line expressing the rhIL-2 at a very high level, about $11.4\mug/10^6$ cell/day, which is about 6 times higher than that previously reported. The biological activity of the rhIL-2 protein purified from the cell line was also confirmed by the cell proliferation assay.

• Bulletin of the Korean Space Science Society
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• 2009.10a
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• pp.26.2-26.2
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• 2009

The Solar and Space Weather Research Group (SOS) in Korea Astronomy and Space Science Institute (KASI) is constructing the Space Weather Prediction Center since 2007. As a part of the project, we are developing automatic real-time system of the global 3-D magnetohydrodynamics (MHD) simulation. The MHD simulation model of earth's magnetosphere is designed as modified leap-frog scheme by T. Ogino, and it was parallelized by using message passing interface (MPI). Our work focuses on the automatic processing about simulation of 3-D MHD model and visualization of the simulation results. We used PC cluster to compute, and virtual reality modeling language (VRML) file format to visualize the MHD simulation. The system can show the variation of earth's magnetosphere by the solar wind in quasi real time. For data assimilation we used four parameters from ACE data; density, pressure, velocity of solar wind, and z component of interplanetary magnetic field (IMF). In this paper, we performed some initial tests and made a animation. The automatic real-time system will be valuable tool to understand the configuration of the solar-terrestrial environment for space weather research.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.103.1-103.1
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• 2012

We present the results of SDSS r, i and z band photometry for HBC722 (also known as $LkH{\alpha}$ 188 G4, PTF10qpf and V2493 Cyg), with Camera for Quasars in Early uNiverse (CQUEAN) attached to 2.1m Otto Struve telescope at McDonald Observatory, USA. HBC722 is a newly erupted FU Orionis type object, which produced optical outburst (${\Delta}V$=4.7 mag) over a year that peaked in 2010 September. We carried out the monitoring observations during 48 nights from 2011 April to 2012 June to check the short-term and the long-term variabilities for chasing the Keplerian rotation of the system. Comparing the photometric results of r, i and z band, we describe the color variability which is related to the physical properties of the system like circumstellar disk and accretion process.

• Nuclear Engineering and Technology
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• v.50 no.3
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• pp.319-326
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• 2018

Since the Fukushima Daiichi nuclear disaster, concern and worry about multiunit accidents have been increasing. Korea has a higher urgency to evaluate its site risk because its number of nuclear power plants (NPPs) and population density are higher than those in other countries. Since the 1980s, technical documents have been published on multiunit probabilistic safety assessment (PSA), but the Fukushima accident accelerated research on multiunit PSA. It is therefore necessary to summarize the present situation and draw implications for further research. This article reviews journal and conference papers on multiunit or site risk evaluation published between 2011 and 2016. The contents of the reviewed literature are classified as research status, initiators, and methodologies representing dependencies, and the insights and conclusions are consolidated. As of 2017, the regulatory authority and nuclear power utility have launched a full-scale project to assess multiunit risk in Korea. This article provides comprehensive reference materials on the necessary enabling technology for subsequent studies of multiunit or site risk assessment.

• The Bulletin of The Korean Astronomical Society
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• v.45 no.1
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• pp.41.1-41.1
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• 2020

Different molecular lines trace different physical environments (with various densities and temperatures) within molecular clouds (MCs). Therefore, multimolecular line observations are crucial to study the physical and chemical structures of MCs. We observed the Orion A and Ophiuchus clouds in six different molecular lines as a Taeduk Radio Astronomy Observatory Key Science Program (TRAO-KSP), "mapping Turbulent properties In star-forming MolEcular clouds down to the Sonic scale" (TIMES; PI: Jeong-Eun Lee). Here, we investigate the characteristic relations between the observed lines by performing the Principal Component Analysis (PCA). We also investigate the correlation between the line intensity distributions and the physical parameters, such as the gas column density and dust temperature. Finally, we will discuss how the correlations among different chemical tracers vary with the star formation environments.