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Development of Perilla frutescens with Low Levels of Alpha-Linolenic Acid by Inhibition of a delta 15 desaturase Gene (Delta 15 desaturase 유전자 억제에 의해 알파리놀렌산 함량이 낮은 들깨 육성)

  • Kim, Kyung-Hwan;Lee, Kyeong-Ryeol;Kim, Jung-Bong;Lee, Myoung Hee;Lee, Eungyeong;Kim, Nyunhee;Lee, Hongseok;Kim, Song Lim;Baek, JeongHo;Choi, Inchan;Ji, Hyeonso
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.463-471
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    • 2018
  • Perilla is an oilseed crop cultivated in Korea since ancient times. Due to the high ${\alpha}-linolenic$ acid content in perilla, perilla seed oil can easily become rancid. ${\alpha}-Linolenic$ acid is synthesized by two enzymes, endoplasmic reticulum-localized ${\Delta}15$ desaturase (FAD3) and chloroplast-localized ${\Delta}15$ desaturase (FAD7) in vivo. In order to lower the ${\alpha}-linolenic$ acid content of the seed oil without disturbing plant growth, we tried to suppress the expression of only the FAD3 gene using RNA interference, whilst maintaining the expression of the FAD7 gene. Seventeen transgenic plants with herbicide ($Basta^{TM}$) resistance were obtained by Agrobacterium-mediated transformation using hypocotyls of perilla plants. The transgenic plants were firstly confirmed by treatment with 0.3% (v/v) $Basta^{TM}$ herbicide, and the expression of FAD3 was measured by Northern blot analysis. The ${\alpha}-linolenic$ acid content was 10-20%, 30-40%, and 60% in two, seven, and three of the twelve $T_1$ transgenic perilla plants which had enough seeds to be analyzed for fatty acid composition, respectively. Analysis of the fatty acid composition of $T_2$ progeny seeds from $T_1$ plants with the lowest ${\alpha}-linolenic$ acid content showed that the homozygous lines had 6-10% ${\alpha}-linolenic$ acid content and the heterozygous lines had 20-26% ${\alpha}-linolenic$ acid content. It is expected that the reduction in ${\alpha}-linolenic$ acid content in perilla seed oil will prevent rancidity and can be utilized for the production of high-value functional ingredients such as high ${\gamma}-linolenic$ acid.

Soil Physical and Chemical Properties of Kaolinite Opencast Mines and Adjacent Red Pine Forests in Sancheong-gun (산청군 고령토(백토) 노천 광산 채굴지와 인접 소나무 임분의 토양 물리·화학적 성질)

  • Kim, Kyung Tae;Baek, Gyeongwon;Choi, Byeonggil;Ha, Jiseok;Kim, Choonsig
    • Journal of Korean Society of Forest Science
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    • v.109 no.4
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    • pp.382-389
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    • 2020
  • Soil properties in opencast mines are a key factor in reclamation (revegetation) of mining areas. In this study we determined the soil physical and chemical properties of kaolinite tailings, reclaimed areas, and adjacent natural red pine (Pinus densiflora S. et Z.) forests in kaolinite opencast mines in Sancheong-gun, Gyeongsangnam-do. Six sites were chosen for collection of soil samples to determine soil physical and chemical properties at a soil depth of 10 cm. Soil bulk density was significantly higher (P < 0.05) in the kaolinite tailings (1.51 g·cm-3) than in the reclaimed areas (1.19 g·cm-3) and red pine forests (0.93 g·cm-3), whereas air phase in the kaolinite tailings (14.2%) was significantly lower than in the red pine forests (32.6%). Clay content in the red pine forests was significantly higher than in the reclaimed areas (18.7%) or kaolinite tailings (14.8%), whereas soil structural stability index was significantly lower in the reclaimed areas (1.61%) and kaolinite tailings (0.87%) than in the red pine forests (7.75%). Soil pH was significantly higher in the kaolinite tailings (pH 6.68) and reclaimed areas (pH 6.27) than in the red pine forests (pH 5.31). Soil organic carbon and total nitrogen were significantly higher in the red pine forests (C: 36.03 mg·g-1; N: 2.08 mg·g-1) than in the reclaimed areas (C: 5.00 mg·g-1; N: 0.31 mg·g-1) than in the kaolinite tailings (C: 2.12 mg·g-1; N: 0.07 mg g-1). The amount of available phosphorus was not significantly different among the three treatments. The concentration of exchangeable potassium was significantly lower in the kaolinite tailings (0.08 cmolc·kg-1) than in the reclaimed areas (0.21 cmolc·kg-1) and red pine forests (0.30 cmolc·kg-1). These results indicate that, because of high soil bulk density and low soil organic carbon, total nitrogen, available phosphorus, and exchangeable potassium in kaolinite tailings and reclaimed mining areas, soil nutrient management is needed in order to reclaim the vegetation in these type of areas.

Anti-hyperlipidemic and anti-obesity effects of Sparassis latifolia fruiting bodies in high-fat and cholesterol-diet-induced hyperlipidemic rats (고지방과 고콜레스테롤 식이 급여에 의해 고지혈증이 유도된 흰쥐에서 꽃송이버섯 자실체의 항고지혈증과 항비만 효과)

  • Im, Kyung-Hoan;Baek, Seung-A;Choi, Jaehyuk;Lee, Tae-Soo
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.23-32
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    • 2021
  • This study investigated the anti-hyperlipidemic and anti-obesity effects of Sparassis latifolia (S. latifolia) fruiting body powder in rats fed with a high fat and cholesterol diet (HFD). Rats were fed a normal control diet (ND), an HFD, an HFD supplemented with 5% fruiting body powder of S. latifolia (HFD+SL), or an HFD supplemented with 0.03% simvastatin (HFD+SS), for 6 weeks. The HFD group demonstrated considerable increase in body weight gain, the food efficiency ratio (FER), and plasma cholesterol and triglyceride levels, compared to the ND group. In contrast, the HFD+SL and HFD+SS groups showed significantly reduced body weight gain, food intake, and plasma cholesterol and triglyceride levels compared to the HFD group. In particular, the HFD+SL and HFD+SS diets significantly suppressed the occurrence of non-alcoholic fat deposits in the liver. Taken together, these results suggest that dietary supplementation of the fruiting body powder of S. latifolia in an HFD could lower the risks of hyperlipidemia, atherogenesis, and obesity and may be used as a functional food to manage cardiovascular disease and fecal lipid and cholesterol levels.

Development of Prediction Model for the Na Content of Leaves of Spring Potatoes Using Hyperspectral Imagery (초분광 영상을 이용한 봄감자의 잎 Na 함량 예측 모델 개발)

  • Park, Jun-Woo;Kang, Ye-Seong;Ryu, Chan-Seok;Jang, Si-Hyeong;Kang, Kyung-Suk;Kim, Tae-Yang;Park, Min-Jun;Baek, Hyeon-Chan;Song, Hye-Young;Jun, Sae-Rom;Lee, Su-Hwan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.4
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    • pp.316-328
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    • 2021
  • In this study, the leaf Na content prediction model for spring potato was established using 400-1000 nm hyperspectral sensor to develop the multispectral sensor for the salinity monitoring in reclaimed land. The irrigation conditions were standard, drought, and salinity (2, 4, 8 dS/m), and the irrigation amount was calculated based on the amount of evaporation. The leaves' Na contents were measured 1st and 2nd weeks after starting irrigation in the vegetative, tuber formative, and tuber growing periods, respectively. The reflectance of the leaves was converted from 5 nm to 10 nm, 25 nm, and 50 nm of FWHM (full width at half maximum) based on the 10 nm wavelength intervals. Using the variance importance in projections of partial least square regression(PLSR-VIP), ten band ratios were selected as the variables to predict salinity damage levels with Na content of spring potato leaves. The MLR(Multiple linear regression) models were estimated by removing the band ratios one by one in the order of the lowest weight among the ten band ratios. The performance of models was compared by not only R2, MAPE but also the number of band ratios, optimal FWHM to develop the compact multispectral sensor. It was an advantage to use 25 nm of FWHM to predict the amount of Na in leaves for spring potatoes during the 1st and 2nd weeks vegetative and tuber formative periods and 2 weeks tuber growing periods. The selected bandpass filters were 15 bands and mainly in red and red-edge regions such as 430/440, 490/500, 500/510, 550/560, 570/580, 590/600, 640/650, 650/660, 670/680, 680/690, 690/700, 700/710, 710/720, 720/730, 730/740 nm.

Effects of Halogen and Light-Shielding Curtains on Acquisition of Hyperspectral Images in Greenhouses (온실 내 초분광 영상 취득 시 할로겐과 차광 커튼이 미치는 영향)

  • Kim, Tae-Yang;Ryu, Chan-Seok;Kang, Ye-seong;Jang, Si-Hyeong;Park, Jun-Woo;Kang, Kyung-Suk;Baek, Hyeon-Chan;Park, Min-Jun;Park, Jin-Ki
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.23 no.4
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    • pp.306-315
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    • 2021
  • This study analyzed the effects of light-shielding curtains and halogens on spectrum when acquiring hyperspectral images in a greenhouse. The image data of tarp (1.4*1.4 m, 12%) with 30 degrees of angles was achieved three times with four conditions depending on 14 heights using the automatic image acquisition system installed in the greenhouse at the department of Southern Area of National Institute of Crop Science. When the image was acquired without both a light-shielding curtain and halogen lamp, there was a difference in spectral tendencies between direct light and shadow parts on the base of 550 nm. The average coefficient of variation (CV) for direct light and shadow parts was 1.8% and 4.2%, respective. The average CV value was increased to 12.5% regardless of shadows. When the image was acquired only used a halogen lamp, the average CV of the direct light and shadow parts were 2 .6% and 10.6%, and the width of change on the spectrum was increased because the amount of halogen light was changed depending on the height. In the case of shading curtains only used, the average CV was 1.6%, and the distinction between direct light and shadows disappeared. When the image was acquired using a shading curtain and halogen lamp, the average CV was increased to 10.2% because the amount of halogen light differed depending on the height. When the average CV depending on the height was calculated using halogen and light-shielding curtains, it was 1.4% at 0.1m and 1.9% at 0.2 m, 2 .6% at 0.3m, and 3.3% at 0.4m of height, respectively. When hyperspectral imagery is acquired, it is necessary to use a shading curtain to minimize the effect of shadows. Moreover, in case of supplementary lighting by using a halogen lamp, it is judged to be effective when the size of the object is less than 0.2 m and the distance between the object and the housing is kept constant.

Landslide Susceptibility Mapping Using Deep Neural Network and Convolutional Neural Network (Deep Neural Network와 Convolutional Neural Network 모델을 이용한 산사태 취약성 매핑)

  • Gong, Sung-Hyun;Baek, Won-Kyung;Jung, Hyung-Sup
    • Korean Journal of Remote Sensing
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    • v.38 no.6_2
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    • pp.1723-1735
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    • 2022
  • Landslides are one of the most prevalent natural disasters, threating both humans and property. Also landslides can cause damage at the national level, so effective prediction and prevention are essential. Research to produce a landslide susceptibility map with high accuracy is steadily being conducted, and various models have been applied to landslide susceptibility analysis. Pixel-based machine learning models such as frequency ratio models, logistic regression models, ensembles models, and Artificial Neural Networks have been mainly applied. Recent studies have shown that the kernel-based convolutional neural network (CNN) technique is effective and that the spatial characteristics of input data have a significant effect on the accuracy of landslide susceptibility mapping. For this reason, the purpose of this study is to analyze landslide vulnerability using a pixel-based deep neural network model and a patch-based convolutional neural network model. The research area was set up in Gangwon-do, including Inje, Gangneung, and Pyeongchang, where landslides occurred frequently and damaged. Landslide-related factors include slope, curvature, stream power index (SPI), topographic wetness index (TWI), topographic position index (TPI), timber diameter, timber age, lithology, land use, soil depth, soil parent material, lineament density, fault density, normalized difference vegetation index (NDVI) and normalized difference water index (NDWI) were used. Landslide-related factors were built into a spatial database through data preprocessing, and landslide susceptibility map was predicted using deep neural network (DNN) and CNN models. The model and landslide susceptibility map were verified through average precision (AP) and root mean square errors (RMSE), and as a result of the verification, the patch-based CNN model showed 3.4% improved performance compared to the pixel-based DNN model. The results of this study can be used to predict landslides and are expected to serve as a scientific basis for establishing land use policies and landslide management policies.

Structuralization of Elective Courses in High School Home Economics(Subject Group) in Preparation for the Next Curriculum (차기 교육과정을 대비한 고등학교 가정교과(군) 선택과목의 구조화)

  • Yu, Nan Sook;Baek, Min Kyung;Ju, Sueun;Han, Ju;Park, Mi Jeong
    • Journal of Korean Home Economics Education Association
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    • v.33 no.1
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    • pp.129-149
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    • 2021
  • The purposes of this study were to examine the current status of the establishment of home economics-related departments in colleges and universities and the changes required in the home economics curriculum of secondary schools, and to structure the elective courses of home economics subject(group) that can be organized in the next high school curriculum. To achieve these purposes, related literature and data were analyzed, and a questionnaire survey and FGI were conducted by home economics experts. The research results are as follows. First, home economics was considered to be highly related not only to the human ecology but also to social sciences, education, engineering, and arts and physical education. The numbers of technical colleges and 4-year universities with departments related to home economics were 1,405 and 961 respectively in 2019. Therefore, it was confirmed that there is a sufficient basis for opening home economics subject(group) elective courses in high school. Second, in the secondary school home economics curriculum, the concepts of culture, relations, independence, and sustainability were emphasized based on the changing life patterns and values. It was proposed that the contents of the home economics course would be structured in a way that allows deep and high-level thinking and helps students to enjoy culture. This demand can be implemented by diversifying, specializing, and structuring the elective courses of the home economics subject(group). Third, a total of 18 elective subjects and subject outlines were structured in the fields of child/family, food/nutrition, clothing, housing, consumption/family management, and home economics integration. This study results will contribute to the establishment of the high school credit system by providing basic information for organizing the next home economics curriculum, and expanding the options for home economics subject(group) to high school students.

Proposal of Joint Planning Working Group for Development of Korean Space Telescopes (한국형 우주망원경 개발을 위한 공동기획 Working Group 제안)

  • Han, Jeong-Yeol;Park, Woojin;Jun, Youra;Kim, Jihun;Kim, Yunjong;Choi, Seonghwan;Kim, Young-Soo;Baek, Ji-Hye;Moon, Bongkon;Jang, Biho;Kim, Jae-Woo;Hong, Sungwook E.;Jung, Youn Kil;Pak, Soojong;Chung, Soyoung
    • Journal of Space Technology and Applications
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    • v.1 no.3
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    • pp.283-301
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    • 2021
  • In order to satisfy the intellectual curiosity of mankind to explore the unknown, National Aeronautics and Space Administration (NASA) in the United States and European Space Agency (ESA) in Europe are embarking on various R&D under the motto of the grand dream of pioneering space into a safe and sustainable environment. In the 2020s and 30s, it is expected that advanced giant observation equipment will be in operation, such as the development of a 10-meter-class telescope in space. In Korea, following the development of the 0.15 m Near-Infrared Imaging Spectrometer (NISS), Korea Astronomy and Space Science Institute (KASI) is also participating a 0.2 m Spectro-Photometer for the History of the Universe, Epoch of Reionization, and Ices Explorer (SPHEREx) as an international cooperation partner in small exploration telescope. However, domestic experience in the development and operation of the space telescopes is still insufficient, and there is no plan with long-term prospects for constructing telescopes. In order to answer questions about the unknown world that mankind has not experienced using our own equipment, planning and preparation for the construction of a space telescope through close cooperation among industry-university-institute-government is urgently needed. In this paper, the necessity, background, development goals, and expected effects of the development of the Korean Space Telescope are summarized conceptually, and a working group (WG) is also proposed. In the WG activities, Korea shall take the lead in establishing the Korean-style space telescope development plan, and will start a valuable step to establish the national direction in the field of space astronomy and related technologies. We hope that the WG will be another milestone in Korea's space development.

Analysis of Land Use Characteristics Using GIS DB - A Case Study of Busan Metropolitan City in Korea - (GIS DB를 이용한 토지이용 특성 분석 - 부산광역시 건물 높이 시뮬레이션을 중심으로 -)

  • Min-Kyoung CHUN;Tae-Kyung BAEK
    • Journal of the Korean Association of Geographic Information Studies
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    • v.26 no.3
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    • pp.52-64
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    • 2023
  • As cities continue to develop rapidly, overcrowding, pollution, and urban sanitation problems arise, and the need to separate conflicting uses is emerging. From this perspective, there is no disagreement that urban land use should be planned. Therefore, all activities in land space must be predicted in advance and planned so that land use can be rationally established. This study used the constructed data to compare and analyze the use distribution characteristics of residential, commercial, and industrial areas in Busan Metropolitan City to identify the building area status, total floor area, and floor area ratio by use zone in districts and counties in Busan Metropolitan City. As a result, it was found that the residential area accounted for the largest proportion of the area by use zone at 51%, and that the residential area accounted for the largest proportion at 63% of the total floor area by use zone. And the analysis was conducted using a specialization coefficient that can identify regional characteristics based on land use composition ratio. Because it is difficult to determine the trend of the entire region just by counting the absolute value of the area, the area composition ratio was calculated and compared. Looking at the residential facilities among the specialization coefficients by use area, it is above 1.0 except for Gijang-gun, Sasang-gu, Saha-gu, and Jung-gu. Commercial facilities are over 1.0 except for Gijang-gun, Gangseo-gu, Nam-gu, Sasang-gu, and Saha-gu. Looking at industrial facilities, you can see that the industrial complex distribution area is Gangseo-gu (2.5), Gijang-gun (1.22), Sasang-gu (2.06), and Saha-gu (1.64). In addition, it was found that business facilities and educational welfare facilities were evenly distributed. Land use analysis was conducted through simulation of the current status of building heights according to each elevation in each use area and the height of buildings in each use area. In general, areas over 80m account for more than 43% of Busan City, showing that the distribution of use areas is designated in areas with high altitude due to the influence of topographical conditions.

Evaluation and Verification of the Attenuation Rate of Lead Sheets by Tube Voltage for Reference to Radiation Shielding Facilities (방사선 방어시설 구축 시 활용 가능한 관전압별 납 시트 차폐율 성능평가 및 실측 검증)

  • Ki-Yoon Lee;Kyung-Hwan Jung;Dong-Hee Han;Jang-Oh Kim;Man-Seok Han;Jong-Won Gil;Cheol-Ha Baek
    • Journal of the Korean Society of Radiology
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    • v.17 no.4
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    • pp.489-495
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    • 2023
  • Radiation shielding facilities are constructed in locations where diagnostic radiation generators are installed, with the aim of preventing exposure for patients and radiation workers. The purpose of this study is seek to compare and validate the trend of attenuation thickness of lead, the primary material in these radiation shielding facilities, at different maximum tube voltages by Monte Carlo simulations and measurement. We employed the Monte Carlo N-Particle 6 simulation code. Within this simulation, we set a lead shielding arrangement, where the distance between the source and the lead sheet was set at 100 cm and the field of view was set at 10 × 10 cm2. Additionally, we varied the tube voltages to encompass 80, 100, 120, and 140 kVp. We calculated energy spectra for each respective tube voltage and applied them in the simulations. Lead thicknesses corresponding to attenuation rates of 50, 70, 90, and 95% were determined for tube voltages of 80, 100, 120, and 140 kVp. For 80 kVp, the calculated thicknesses for these attenuation rates were 0.03, 0.08, 0.21, and 0.33 mm, respectively. For 100 kVp, the values were 0.05, 0.12, 0.30, and 0.50 mm. Similarly, for 120 kVp, they were 0.06, 0.14, 0.38, and 0.56 mm. Lastly, at 140 kVp, the corresponding thicknesses were 0.08, 0.16, 0.42, and 0.61 mm. Measurements were conducted to validate the calculated lead thicknesses. The radiation generator employed was the GE Healthcare Discovery XR 656, and the dosimeter used was the IBA MagicMax. The experimental results showed that at 80 kVp, the attenuation rates for different thicknesses were 43.56, 70.33, 89.85, and 93.05%, respectively. Similarly, at 100 kVp, the rates were 52.49, 72.26, 86.31, and 92.17%. For 120 kVp, the attenuation rates were 48.26, 71.18, 87.30, and 91.56%. Lastly, at 140 kVp, they were measured 50.45, 68.75, 89.95, and 91.65%. Upon comparing the simulation and experimental results, it was confirmed that the differences between the two values were within an average of approximately 3%. These research findings serve to validate the reliability of Monte Carlo simulations and could be employed as fundamental data for future radiation shielding facility construction.