• Journal of fish pathology
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• v.13 no.1
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• pp.37-44
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• 2000

CT(Sodium N-chloro-para toluenesulfonamide, Chloramine-T) known to be a strong oxidative agent was investigated to use as a candidate of disinfectant in the marine aquaculture industry by the analysis of the bactericidal activity against different aquatic microorganisms. One hundred percent mortality appeared at and above 16 ppm CT in sea water in flounder, the predominant species in the marine aquaculture of Korea, when exposed for 48hr. However, bactericidal activity was appeared to be very effective, and all different species of the fish pathogenic bacteria exposed to CT of less than 2 ppm in sea water were dead within 15 min. It allowed us to confirm that CT could be a very effective disinfectant to protect the spread of fish pathogenic bacteria derived from diseased fish or sea water in marine aquaculture. High concentration(10 ppm) with longer exposing time(24 hrs) was required for the algicidal activity of CT, at which concentration might induce the acute toxicity against fish, however, restricted the expanded use of CT for the elimination of zooplanktons at phytoplanktons in marine farms. Moreover the bactericidal activity of CT inhibited almost completely in the present of more than 10 ppm of organic materials pointed out that quality of the culturing sea water should be considered carefully for the application CT to the aquatic farms as a therapeutic agent.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.1-13
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• 1995

Chlorhexidine and Listerine are widely used in dentistry due to its effectiveness on plaque control and bactericidal action. The effects of these agent on chronic gingivitis and wound healing following surgical periodontal therapy in human has been favorable. Understanding the effects of chlorhexidine and Listerine on human gingival fibroblast will provide the rationale for its use during the healing process of periodontal surgery. The purpose of this study was to compare the effects of chlorhexidine and Listerine on human gingival fibroblast. Human gingival fibroblasts were cultured from the healthy gingiva on the extracted premolar of orthodontic patients. Human gingival fibroblast were trypsinized and cultured in growth medium added range of 0.0012-0.12% chlorhexidine and 1-100% Listerine mouth wash solution. The cell used in this study were between fifth to eighth passage number. The cell morphology were examined by inverted microscope and the cell activity were measured by MIT assay. The Morphology of gingival fibroblast added Chlorhexidine and Listerine at the concentration of all range were became globular and lost their cytoplasmic process. Our results indicate that a 0.0012 concentration of chlorhexidine and 1% concentration of Listerine were shows minimal cytotoxicity, but above these concentraion, there was a significant difference between the cell activity in the experimental group and control group(p