• Title/Summary/Keyword: Bacterial distribution

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Cellular Automaton Models Revealing Effects of Initial Bacterial Distribution on Biofilm Growth (생물막 성장에 대한 세균의 초기 분포영향을 나타내는 셀룰라오토마톤 모델)

  • Lee, Sang-Hee;Choi, Kyung-Hee;Chon, Tae-Soo
    • Korean Journal of Ecology and Environment
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    • v.37 no.3 s.108
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    • pp.297-303
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    • 2004
  • Two dimensional cellular automaton (CA) models were developed to investigate growth of biofilms in aquatic ecosystems. Simple local rules on CA were applied to governing growth of bacterial populations in relation to different nutrient concentrations. Initial bacterial distribution played an important role in determining population size and morphology of biofilm at low concentrations of nutrition. With clumped distribution, population size increased slowly compared with uniform and random distributions, while the porosity tented to be higher with uniform distribution compared with other initial distributions.

Molecular Phylogenetic Diversity and Spatial Distribution of Bacterial Communities in Cooling Stage during Swine Manure Composting

  • Guo, Yan;Zhang, Jinliang;Yan, Yongfeng;Wu, Jian;Zhu, Nengwu;Deng, Changyan
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.6
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    • pp.888-895
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    • 2015
  • Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.

Characterizations of Assimilable Organic Carbon, Biodegradable Dissolved Organic Carbon, and Bacterial Regrowth in Distribution Systems by Water Treatment (배수관망에서 수처리에 의한 AOC, BDOC및 세균성장의 특성)

  • Chang, Young-Cheol;Kweon Jung;Yoo, Young-Sik;Kang, Mi-Hye;Andrew A. Randall
    • Journal of Environmental Health Sciences
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    • v.28 no.5
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    • pp.42-52
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    • 2002
  • Two full-scale distribution systems, one treating water by ozonation and another treating water by nanofiltration in parallel with lime softening, were monitored for bacterial growth. Both systems kept disinfectant residuals surf as chlorine and chloramine in their respective distribution systems. Bacterial growth was assessed by heterotrophic plate counts (HPC) on R2A agar. In the distribution systems fed by ozonated water, HPCs were correlated ($R^2$= 0.97) using an exponential model with the assimilable organic carbon (AOC) at each sampling site. Also, it was observed that ozonation caused a significant increase in the AOC concentration of the distribution system (over 100% increase) as well as a significant increase in the bacterial counts of the distribution system (average increase over 100%). The HPCs from the distribution systems fed by nanofiltration in parallel with lime-softening water also displayed an exponential correlation ($R^2$ = 0.75) with an exponential model based on AOC. No significant correlation was found between bacteria growth on R2A agar and BDOC concentrations. Therefore, in agreement with previous work, bacterial growth in the distribution systems was found to correlate with AOC concentrations.

Phage Typing and Lysotype Distribution of Xanthomonas axonopodis pv, citri, the Causal Agent of Citrus Bacterial Canker in Korea

  • Myung, Inn-Shik;Yongsup Cho;Lee, Young-Hee;Kwon, Hyuk-Mo
    • The Plant Pathology Journal
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    • v.17 no.6
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    • pp.336-341
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    • 2001
  • The distribution of citrusphages and phage types of Xanthomonas axonopodis pv. citri was investigated in Korea. Forty-eight strains of the bacterial pathogen and 28 bacteriophage strains were isolated from citrus leaves showing the citrus canker symptom. Only a single bacteriophage group, named CPK, was identified based on their aggressiveness to the bacterial pathogen. The bacterial strains were differentiated into two Iysotypes based on their sensitivity to CPK. Lysotype I, which was sensitive to CPK, was more predominant (96%), while only 4% belonged to Iysotype II, which was resistant to CPK. Among the 13 xanthomonads including Iysotype A and Iysotype B of X axonopodis pv. citri, CPKs were only aggressive to BC 83 (=Xc 62) strain of X. axonopodis pv, citri reported as Iysotype A. Thus, bacterial pathogens and citrusphages related to citrus plants mainly distributed in Korea were presumed as Iysotype A of X. axonopodis pv, citri, and Iysotype A-infecting CP$_1$ respectively.

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Abundance and Structure of Microbial Loop Components (Bacteria and Protists) in Lakes of Different Trophic Status

  • Chrost, Ryszard J.;Tomasz, Adamczewski;Kalinowska, Krystyna;Skowronska, Agnieszka
    • Journal of Microbiology and Biotechnology
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    • v.19 no.9
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    • pp.858-868
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    • 2009
  • The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic water layer during summer stratification periods. Image analysis techniques with fluorescent in situ hybridization (FISH) as well as [$^3H$]-methyl-thymidine incorporation methods were applied to analyze differences in the composition and activity of bacterial communities. The greatest differences in trophic parameters were found between the humic lake and remaining non-humic ones. The same bacterial and heterotrophic nanoflagellate (HNF) cell size classes dominated in all the studied lakes. However, distinct increases in the contributions of large bacterial (>$1.0{\mu}m$) and HNF (>$10{\mu}m$) cells were observed in eutrophic lakes. The bacterial community was dominated by the ${\beta}$-Proteohacteria group, which accounted for 27% of total DAPI counts. Ciliate communities were largely composed of Oligotrichida. Positive correlations between bacteria and protists, as well as between nanoflagellates (both heterotrophic and autotrophic) and ciliates, suggest that concentrations of food sources may be important in determining the abundance of protists in the studied lakes.

Resistance of Biofilm Bacteria to Chlorination (생물막 세균의 염소소독제에 대한 내성)

  • 조재창;박성주;김상종
    • Korean Journal of Microbiology
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    • v.31 no.3
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    • pp.255-260
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    • 1993
  • The Enterobacter cloacae biofilms developed on slide glasses and galvanized-iron coupons were applied to test the attached bacterial resistance to chlorination. The chlorine resistances of biofilm bacteria grown on the slide glasses and galvanized-iron coupons were 14 and 480 times that of the suspended bacteria, respectively. The chlorine resistance of particleattached bacterial populations was 48 times that of suspended bacterial populations. The biofilm bacterial densities developed on the slide glasses and galvanized-iron coupons which were immersed in the flowing tap water for 75 days were $4.75 {\times} 10^{4}$ and $1.12 {\times} 10^5 cfu/cm^{2}$ It is concluded that main mechanisms of enteric or HPC bacterial resistance to chlorination in tap waters are bacterial attachment or . adsorption to particles or bacterial aggregations and formation of biofilms on the inner wall of distribution systems by escaped bacteria from chlorination in water treatment processes, which results in bacterial regrowth in water distribution systems.

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Seroprevalence of Q-fever in Korean native cattle (국내 서식 한우에서 큐열 항체 양성율 조사)

  • Kim, Ji-Yeon;Sung, So-Ra;Pyun, Ji-In;Her, Moon;Kang, Sung-Il;Lee, Hyang-Keun;Jung, Suk Chan
    • Korean Journal of Veterinary Research
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    • v.54 no.3
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    • pp.147-150
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    • 2014
  • Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.

Bacterial Communities from the Water Column and the Surface Sediments along a Transect in the East Sea

  • Lee, Jeong-Kyu;Choi, Keun-Hyung
    • Journal of Marine Life Science
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    • v.6 no.1
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    • pp.9-22
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    • 2021
  • We determined the composition of water and sediment bacterial assemblages from the East Sea using 16S rRNA gene sequencing. Total bacterial reads were greater in surface waters (<100 m) than in deep seawaters (>500 m) and sediments. However, total OTUs, bacterial diversity, and evenness were greater in deep seawaters than in surface waters with those in the sediment comparable to the deep sea waters. Proteobacteria was the most dominant bacterial phylum comprising 67.3% of the total sequence reads followed by Bacteriodetes (15.8%). Planctomycetes, Verrucomicrobia, and Actinobacteria followed all together consisting of only 8.1% of the total sequence. Candidatus Pelagibacter ubique considered oligotrophic bacteria, and Planctomycetes copiotrophic bacteria showed an opposite distribution in the surface waters, suggesting a potentially direct competition for available resources by these bacteria with different traits. The bacterial community in the warm surface waters were well separated from the other deep cold seawater and sediment samples. The bacteria exclusively associated with deep sea waters was Actinobacteriacea, known to be prevalent in the deep photic zone. The bacterial group Chromatiales and Lutibacter were those exclusively associated with the sediment samples. The overall bacterial community showed similarities in the horizontal rather than vertical direction in the East Sea.

E. Coli Bacterial Foraging Optimization based Distribution Nonconvex Economic Dispatch Algorithm (E. Coli Bacterial Foraging 최적화에 기준한 배전용 Nonconvex 경제급전 알고리즘)

  • Lee, S.S.;Kim, D.H.;Kim, M.K.;Norbekov, N.;Lee, H.C.;Lee, S.K.;Park, J.K.;Moon, S.I.;Yoon, Y.T.
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.569-570
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    • 2007
  • 본 논문에서는 대장균 먹이찾기(E. Coli Bacterial Foraging)를 최적화 기법에 도입한 사례로서 이를 이용하여 Nonconvex 배전용 경제급전 알고리즘(distribution economic dispatch: DED을 제안한다. 제안된 DED 알고리즘은 향후 지역이나 구역 발전사업자를 중심으로 배전계통운용시스템에 필요한 경제급전 알고리즘으로 활용 할 수 있다.

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