• Title/Summary/Keyword: Bacterial Inoculation

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Erwinia carotovora subsp. carotovora에 의한 구약감자 무름병(신칭) (Bacterial Soft Rot of Elephant Foot Caused by Erwinia carotovora subsp. carotovora)

  • 최재을;박종성;인무성;안병창
    • 한국식물병리학회지
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    • 제3권3호
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    • pp.236-238
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    • 1987
  • A bacterial disease of elephant foot, Hydrosome rivieri Engl., was newly found in Taejon, Korea in August 1986. The affected plants showed symptoms of soft rot on leaf blades and petioles. Bacterial isolates from affected plants found to be pathogenic to elephant foots by antificial inoculation, producing similar symptoms with those produced naturally. The baterium was also pathogenic to carrot, Chinese cabbage, radish, potato and onion, and developed symptom of soft root on them. On the basis of bacteriological characteristics and pathogenicity, tested bacterium was identified as Erwinia carotovora subsp. carotovora and this disease was proposed to name 'Bacterial soft rot of elephant foot'.

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Xanthomonas arboricora pv. pruni에 의한 복숭아 세균성가지마름병의 발생 (Bacterial Branch Blight of Peach Tree Caused by Xanthomonas arboricora pv. pruni)

  • 김종완
    • 식물병과 농업
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    • 제5권2호
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    • pp.115-118
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    • 1999
  • A new bacterial disease was found on trees of peach(Prunus persica var. vulgaris Max) at Kumho Kyungbuk in April 1999. The disease usually occured on over wintered buds branchs and stems of the Cheon-Hong cultivar. The buds died without sprouting and the branches showed entire wilting Droplets of bacterial ooze was occasionally running down the surface of diseased plants under moist condition. Artificial needle prick inoculation with isolates obtained from branchs of naturally infected plants produced symptoms similar to those occuring under natural condition. On the basis of bacteriological characteristics and pathogenicity on the host plant of the organism the causal bacterium was identified as Xanthomonas arboricora pv. pruni and this disease was proposed to name "Bacterial branch blight of the peach tree"

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Erwinia carotovora subsp. carotovora에 의한 고추 마디 무름병 (Bacterial Node Soft Rot of Pepper (Capsicum annuum L.) Caused by Erwinia carotovora subsp. carotovora)

  • 정기채;임진우;김승한;임양숙;김종완
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.741-743
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    • 1998
  • A bacterial disease of pepper (Capsicum annuum L.) that rooted the stem nodes to black was found in pepper plants which cultivated in plastic house at Chungdo, Kyungpook, Korea in March, 1998. Bacterial isolates derived from the diseased peppers were pathogenic to potato, eggplant and Chinese cabbage but, was not pathogenic to chrysanthemum by artificial inoculation. On the basis of bacteriological characteristics and pathogenicity test on host plants, the causal organism of the node soft rot of pepper is identified as Erwinia carotovora subsp. carotovora and the name of disease is proposed as bacterial node soft rot of pepper.

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고추의 생장에 미치는 Azospirillum brasilense 및 Methylobacterium oryzae 접종 효과 (Effect of Inoculation of Azospirillum brasilense and Methylobacterium oryzae on the Growth of Red Pepper Plant)

  • 김병호;사동민;정종배
    • 한국환경농학회지
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    • 제30권2호
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    • pp.223-228
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    • 2011
  • BACKGROUND: Rhizosphere bacteria may improve plant growth and productivity both by supply nutrients and hormonal stimulation. Although many experiments have shown improvements in plant growth with inoculation of bacterial cultures to the rhizosphere, the main obstacle in the applications of plant growth promoting rhizobacteria in a large scale is the inconsistency of the results. We tested the growth promoting effects of Azospirillum and Methylobacterium strains on red pepper plant. METHODS AND RESULTS: Red pepper seedlings were grown for 25 days in a growth media inoculated with A. brasilense CW903 or M. oryzae CBMB20. The seedlings were transplanted and grown for 45 days in pots with soil in a greenhouse, at half the recommended level of fertilizer. Bacterial culture, $4.0{\times}10^9$ for A. brasilense CW903 and $5.8{\times}10^8$ CFU for M. oryzae CBMB20, was applied in root zone soil periodically every 10 days during the experiment. Inoculation of M. oryzae CBMB20 significantly increased the red pepper plant growth in terms of leaf number, height and mass of shoot, or root mass compared to uninoculated control plants. Although beneficial effects of A. brasilense on plant growth of many crops were observed, the growthpromoting effect of A. brasilense CW903 on red pepper plant was not found in this study. CONCLUSION(s): The factors responsible for the irregularities in plant growth promoting of rhizobacteria are difficult to elucidate. Extensive inoculation experiments in the greenhouse and in the field should enable us to define the factors critical to obtain successful application of plant growth promoting rhizobacteria.

Degradation of Cholesterol by Bacillus subtilis SFF34 in Flatfish during Fermentation

  • Kim, Kwan-Pil;Rhee, In-Koo;Park, Heui-Dong
    • Journal of Microbiology
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    • 제41권4호
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    • pp.284-288
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    • 2003
  • Bacillus subtilis SFF34 degrading cholesterol was applied to reduce residual cholesterol content in fermented flatfish. When the bacterial cells were inoculated as a start culture, a maximal level (1.7 U/g) of cholesterol oxidase was obtained after 10 days, which was two times higher than that (0.8 U/g) without inoculation. Residual cholesterol contents with and without inoculation of the cells were 0.5 mg/g and 0.8 mg/g after 12 days of fermentation, respectively. Cholesterol derivatives including cholesterol- 5${\alpha},\;6{\alpha}$-epoxide, 4-cholesten-3-one and 7${\beta}$-hydroxycholesterol were detected in raw flatfish as well as fermented flatfish. Campesterol and 25-hydroxycholesterol were detected only after fermentation. However, no significant differences in their contents were observed regardless of inoculation.

Erwinia herbicola 의한 Kalanchoe blossfeldiana세균성무름병 (Bacterial Soft rot of Kalanchoe blossfeldiana by Erwinia herbicola in Korea)

  • 최재을;이은정
    • 식물병연구
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    • 제6권1호
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    • pp.15-18
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    • 2000
  • 1998년 대전의 비닐하우스에서 재배중인 칼란코에의 잎에 세균병이발생하였다. 이들 병반은 수침상 병반이 나타나고 병반진전과 함께 무름증상을 보였다. 이들로부터 분리한 병원세균을 침접종한 결과 자연병징과 같은 병반을 형성하였다. 병원세균의 세균학적 특성을 조사한 결과 E. herhicola로 동정되었다. 국내에서는 아직 칼란코에 (Kalanchoe blossfeldiana)의 병원세균으로 E. herbicola가 보고되지 않았다. 따라서 이를 E. herbicola에 의한 \"칼란코에의 세균성 무름병\"으로 명명할 것을 제안한다.할 것을 제안한다.

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MicroTom - A Model Plant System to Study Bacterial Wilt by Ralstonia solanacearum

  • Park, Eun-Jin;Lee, Seung-Don;Chung, Eu-Jin;Lee, Myung-Hwan;Um, Hae-Young;Murugaiyan, Senthilkumar;Moon, Byung-Ju;Lee, Seon-Woo
    • The Plant Pathology Journal
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    • 제23권4호
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    • pp.239-244
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    • 2007
  • MicroTom is a miniature tomato plants with various properties that make it as a model system for experiments in plant molecular biology. To extend its utility as a model plant to study a plant - bacterial wilt system, we investigated the potential of the MicroTom as a host plant of bacterial wilt caused by Ralstonia solanacearum. We compared the disease progress on standard tomato and MicroTom by two inoculation methods, root dipping and soil drenching, using a race 1 strain GMI1000. Both methods caused the severe wilting on MicroTom comparable to commercial tomato plant, although initial disease development was faster in root dipping. From the diseased MicroTom plants, the same bacteria were successfully reisolated using semiselective media to fulfill Koch's postulates. Race specific and isolate specific virulence were investigated by root dipping with 10 isolates of R. solanacearum isolated from tomato and potato plants. All of the tested isolates caused the typical wilt symptom on MicroTom. Disease severities by isolates of race 3 was below 50 % until 15 days after inoculation, while those by isolates of race 1 reached over 50% to death until 15 days. This result suggested that MicroTom can be a model host plant to study R. solanacearum - plant interaction.

Effect of addition of lactic acid bacteria on quality of rye silage harvested at early heading stage

  • Kuppusamy, Palaniselvam;Choi, Ki-Choon;Srigopalram, Srisesharam;Ilavenil, Soundharrajan;Park, Hyung-Su;Jung, Jeong Sung
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.285-285
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    • 2017
  • This study is aimed to analyze the effect of lactic acid bacteria inoculants (LAB) on quality of rye silage fermentation, harvested at early heading stage. The nutritive values were similar between the control and LAB inoculated silages. The pH of rye silage in LAB inoculation significantly decreased as compared to control (p<0.05). In addition, the content of lactic acid in LAB inoculation significantly increased (p<0.05), but the content of acetic acid in LAB treatments decreased. In addition, lactic acid bacterial counts in LAB inoculation significantly increased as compared to control (p<0.05). Therefore, we suggest that rye silage could be improved by novel lactic acid inoculation.

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고정화된 Pantoea agglomrans와 인광석의 복합처리가 벼의 생육 촉진에 미치는 영향 (Effect of Immobilized Cells of Pantoea agglomerans on Growth Promotion of Rice(Oryza sativa L.) in the Presence of Rock Phosphates)

  • 정희경;류정현;이형석;박명수;;;사동민
    • 한국토양비료학회지
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    • 제37권1호
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    • pp.41-45
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    • 2004
  • 충북지역의 근권 토양으로부터 선발한 인산가용화 균인 Pantoea agglomeraans와 인광석을 복합처리하여 벼(Oryza sativa L.)의 생육 및 인산 흡수량에 미치는 영향을 온실내에서 실험하였다. 본 실험은, 종자에 박테리아를 적용시킨 것(bactenzation), 독립 세포 및 고정화된 세포(immobilized cell)를 접종시킨 후, 인광석 1 g과 2.5 g을 각각 시비한 6처리구와 무처리구로 나누어 비교 실험하였다. 인산가용화균을 접종한 처리구가 무처리구에 비하여 벼의 생육 및 인산 흡수량을 증대하는 경향을 나타냈으며, 특히 1 g의 인광석과 고정화된 Pantoea agglomerans를 접종한 처리구에서 다른 처리구들에 비해 가장 우수한 결과를 나타내었다. Pot 충진물내의 유효인산 농도 또한 미생물 접종 처리구에서 증가하였으며, 벼에 흡수된 전인산량과 정의 상관관계가 성립함을 알 수 있었다.