• 제목/요약/키워드: Bacillus identification

검색결과 361건 처리시간 0.021초

Identification of bacteria isolated from rockworm viscera and application of isolated bacteria to shrimp aquaculture wastewater treatment

  • Ja Young Cho;Kyoung Sook Cho;Chang Hoon Kim;Joong Kyun Kim
    • 환경생물
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    • 제41권2호
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    • pp.167-178
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    • 2023
  • Large amounts of waste and wastewater from aquaculture have negatively impacted ecosystems. Among them, shrimp aquaculture wastewater contains large amounts of nitrogen contaminants derived from feed residues in an aerobic environment. This study isolated candidate strains from adult rockworms to treat shrimp aquaculture wastewater (SAW) in an aerobic environment. Among 87 strains isolated, 25 grew well at the same temperature as the shrimp aquaculture with excellent polymer degradation ability (>0.5 cm clear zone). Six isolates (strains AL1, AL4, AL5, AL6, LA10, and PR15) were finally selected after combining strains with excellent polymer degradation ability without antagonism. 16S rRNA sequencing analysis revealed that strains AL1, AL4, AL5, AL6, LA10, and PR15 were closely related to Bacillus paramycoides, Bacillus pumilus, Stenotrophomonas rhizophila, Bacillus paranthracis, Bacillus paranthracis, and Micrococcus luteus, respectively. When these six isolates were applied to SAW, they reached a maximum cell viability of 2.06×105 CFU mL-1. Their chemical oxygen demand (CODCr) and total nitrogen(TN) removal rates for 12h were 51.0% and 44.6%, respectively, when the CODCr/TN ratio was approximately 10.0. Considering these removal rates achieved in this study under batch conditions, these six isolates could be used for aerobic denitrification. Consequently, these six isolates from rockworms are good candidates that can be applied to the field of aquaculture wastewater treatment.

과메기에서 histamine 분해능을 나타내는 세균의 분리 동정 (Isolation and Identification of Histamine Degrading Bacteria from Kwamegi)

  • 김민우;김영만
    • 생명과학회지
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    • 제16권1호
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    • pp.120-125
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    • 2006
  • 과메기에서 histamine분해능을 가진 세균을 분리 동정하기 위하여 histamine만을 첨가한 제한배지에서 균을 분리했다. 기본적인 형태 및 생화학적 동정시험을 거쳐 10종의 시험균주를 선택하여 16S rDNA 염기서열 비교에 의한 계통발생학적 분석으로 동정하였다. 동정된 세균의 histamine분해능은 탁도측정법과 효소법에 의해 재 확인하였으며 그 실험 결과는 다음과 같다. 16S rDNA 염기서열 비교에 의한 계통 발생학적 분석을 이용하여 동정한 결과, E americana B791, Arthrobacter sp. R45S, H. marisflava, P. sp. 9B-7, Bacillus sp. LMG 21002, P. cibarius JG-220, B. megaterium KL-197 7종이 동정되었고, 각각 $99\%,\;95\%,\;98\%,\;99\%,\;99\%,\;99\%,\;98\%$의 상동성을 보였으며, 3종은 미동정되었다. 동정된 세균의 histamine분해능을 탁도측정법과 효소법에 의해 재 확인한 결과, Arthrobacter sp. R45S, H. marisflava, Bacillus sp. LMG 21002, B. megaterium KL-197이 분해능을 나타내었고, Psychrobacter sp. 9B-7은 미약한 분해능을 보였으며 미동정된 3종도 분해능이 있는 것으로 나타났다. 그리고 E. americana B791과 P. cibarius JG-220은 분해능이 불확실한 것으로 확인되었다 자반고등어에서 분리한 균주보다 시험균주 모두가 생육과 histamine의 분해속도가 비교적 빨랐다.

김치에 서식하는 Gram 양성세균의 분리 및 동정의 재평가 (Reevaluation of Isolation and Identification of Gram-positive Bacteria in Kimchi)

  • 임종락;박현근;한홍의
    • 미생물학회지
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    • 제27권4호
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    • pp.404-414
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    • 1989
  • 김치발효 중 Gram 양성세균 또는 유산균의 분리와 동정을 시도하였다. 종의 다양성은 분리배지와 온도에 영양을 받았고, 다양성은 온도가 낮아질수록 감소하는 경향이었다. MRS는 세균의 분리에 KM(김치재료로 만든 자연배지)은 종의 수를 파악 하는데 각각 적합하였다. 분리균의 동정은 Bergey's manual of Systematic Bacterio]ogy (1986)를 기초로 하여 작성한 이 분농생표(dichotomous Identification scheme)에 의하여 선행하였다. 각 온도(5, 15, $25^{\circ}C$)에서 동정된 Gram 양성세균은 Leuconostoc 5종, Streptococcus 4종, Pediococcus 3종, Bacillus 2종 그리고 Ltobacillms 18종이였다. 각 온도에서 출현 빈도가 높은 종은 $25^{\circ}C$에서 LactobaIlus ptantanmz, Streptococcµs faffinolactis, Leuconostoc maιnteroid, subsp mlsentιroides 이었고, $15^{\circ}C$에서 L. mesenteroides Lactobacillus fructosus, L. maen teroid,l.I subsp. mesent,roid, 이였고, $5^{\circ}C$ 에서 Leuconostoc sp.(65.2 빈도)에 의하여 이루어졌다. 그리고 각 온도에 따른 김치발효 중 지금까지 알려진 Pediococcus cerevisiae 와 Streptococcus faecalis는 분리되지 않았다.

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Mannanase를 생산하는 Bacillus sp. WL-3 균주의 분리와 효소 생산성 (Isolation and Enzyme Production of a Mannanase-producing Strain, Bacillus sp. WL-3.)

  • 오영필;이정민;조기행;윤기홍
    • 한국미생물·생명공학회지
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    • 제30권3호
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    • pp.247-252
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    • 2002
  • 전통 발효식품인 장류로부터 분리된 균으로 manna의 분해능이 우수한 Bacillus sp. WL-3는 형태적 특성, 생화학적 성질과 165 rRNA의 염기서열 등이 Bacillus subtilis와 유사성이 높은 것으로 확인되었다. 분리균이 생산하는 mannanase는 $55^{\circ}C$와 pH 6.0에서 최대활성을 보였으며 중성 pH에서 활성도가 높았다. 배지내에 $\alpha$-cellulose, avicel, oat spelt xylan, guar gum 및 locust bean gum(LBG)과 같은 고분자성 탄수화물을 첨가할 경우 mannanase생산성이 증가되었으며, 특히 LBC 0.5%함유한 LB배지에서 9시간 배양하였을 때 배양상등액내의 효소 활성이 65.5 U/ml로 최대 효소 생산성을 나타내 LBC 첨가하지 않은 배지에서 보다 효소 생산성이 131배 이상이 증가하였다. 균이 성장하는 동안 생성된 LBG가수분해 산물이 mannanas리 생합성을 유도하는 것으로 판단된다. 활성 염색을 통하여 Bacillus sp. WL-3의 mannanase를 분석한 결과 배양상등액에는 분자량이 약 38.0 kDa으로 추정되는 한 종류의 mannanase만이 존재하였다

토양으로부터 새로이 분리된 단백질 분해효소 생산 미생물 Bacillus subtilis FBL-1의 동정 (Identification of a Newly Isolated Protease-producing Bacterium, Bacillus subtilis FBL-1, from Soil)

  • 김민아;시진범;위영중
    • 한국미생물·생명공학회지
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    • 제44권2호
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    • pp.185-193
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    • 2016
  • 경상북도 경산시에 위치한 영남대학교 내의 토양으로부터 protease를 생산하는 신규 미생물 FBL-1을 분리하였다. 본 균주는 Biolog test 및 API 50CHB test 결과, Bacillus 속 미생물 중 하나인 것으로 확인되었다. 16S rDNA 염기서열 분석결과로부터 FBL-1 균주는 B. subtilis subsp. subtilis NCIB 3610 (99.5%), B. subtilis subsp. inaquosorum KCTC 13429 (99.4%), B. subtilis subsp. spizizenii NRRL B-23049 (99.3%), B. methylotrophicus KACC 13105 (99.3%) 등과 높은 상동성을 보였다. 이러한 생리적, 형태학적, 계통학적 특성에 따라 균주 FBL-1은 B. subtilis에 속하는 균으로 최종 동정하여 B. subtilis FBL-1으로 명명하였다. B. subtilis FBL-1을 이용한 protease 생산시 탄소원으로는 fructose, 질소원으로는 yeast extract가 균체 성장 및 protease 활성을 위하여 가장 적합한 것으로 나타났다. B. subtilis FBL-1 균주는 protease를 생산하는데 활용할 수 있으며, 향후 배지 및 발효조건 최적화와 효소의 특성 규명 등의 연구를 통하여 식품, 세제 등의 다양한 산업에 유용하게 사용될 수 있을 것으로 기대된다.

Parasporin-4, A Novel Cancer Cell-killing Protein Produced by Bacillus thuringiensis

  • Inouye, Kuniyo;Okumura, Shiro;Mizuki, Eiichi
    • Food Science and Biotechnology
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    • 제17권2호
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    • pp.219-227
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    • 2008
  • Bacillus thuringiensis was isolated as a pathogen of the sotto disease of silkmoth larvae about a hundred years ago. Since then, this bacterium has attracted attentions of not only insect pathologists but also many other scientists who are interested in its strong and specific insecticidal activity. This has led to the recent worldwide development of B. thuringiensis-based microbial insecticides and insect-resistant transgenic plants, as well as a landmark discovery of par asp orin, a cancer cell-specific cytotoxin produced by B. thuringiensis. In this review, we describe examination of interaction between inclusion proteins of B. thuringiensis and brush border membrane of insects using a surface plasmon resonance-based biosensor, identification and characterization of parasporin-4, the latest parasporin produced by the B. thuringiensis A1470 strain, and an effective method for preparing the parasporin-4 from inclusion bodies expressed in the recombinant Escherichia coli cells.

Antifungal Activity of Bacillus sp. Against Phytophthora infestans

  • Kim Hye-Sook;Yi Yong-Sub;Choi Gyung-Ja;Cho Kwang-Yun;Lim Yoong-Ho
    • Journal of Microbiology and Biotechnology
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    • 제16권3호
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    • pp.487-490
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    • 2006
  • Because of consumer rejection of chemical pesticides and the appearance of microorganisms that are resistant to fungicides, we tried to discover biopesticides. Of 13 microorganisms isolated from Shrimp-jeotkal, a Bacillus sp. showed strong activity against tomato late blight caused by Phytophthora infestans. Its activity was tested both in vivo and in vitro. The identification of the strain was carried out based on 16S rDNA analysis and the morphology by scanning electron microscopy.

Postharvest Biological Control of Colletotrichum acutatum on Apple by Bacillus subtilis HM1 and the Structural Identification of Antagonists

  • Kim, Hae-Min;Lee, Kui-Jae;Chae, Jong-Chan
    • Journal of Microbiology and Biotechnology
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    • 제25권11호
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    • pp.1954-1959
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    • 2015
  • Bacillus subtilis HM1 was isolated from the rhizosphere region of halophytes for its antifungal activity against Colletotrichum acutatum, the causative agent of anthracnose. Treatment of postharvest apples with the cell culture or with a cell-free culture supernatant reduced disease severity 80.7% and 69.4%, respectively. Both treatments also exhibited antifungal activity against various phytopathogenic fungi in vitro. The antifungal substances were purified and analyzed by acid precipitation, gel filtration, high-performance liquid chromatography, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Three compounds were identified as fengycin, iturin, and surfactin. The MALDI-TOF/TOF mass spectrum revealed the presence of cyclized fengycin homologs A and B, which were distinguishable on the basis of the presence of either alanine or valine, respectively, at position 6 of the peptide sequence. In addition, the cyclized structure of fengycin was shown to play a critical role in antifungal activity.

Characterization of Bacillus polyfermenticus KJS-2 as a Probiotic

  • Kim, Kang-Min;Kim, Myo-Jeong;Kim, Dong-Hee;Park, You-Soo;Kang, Jae-Seon
    • Journal of Microbiology and Biotechnology
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    • 제19권9호
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    • pp.1013-1018
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    • 2009
  • The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be $60.7{\pm}4.9%$. In addition, treatment of B. polyfermenticus KJS-2 with artificial gastric juice (pH 2.0) and bile acid (pH 6.5) for 4 h resulted in a final viability of $140{\pm}7.9%$ and $108{\pm}3.5%$, respectively. Finally, the results of adhesion experiments using Caco-2 cells revealed that the adherence of B. polyfermenticus KJS-2 to Caco-2 cells was approximately $65{\pm}0.6%$.

Detection of Virulence-Associated Genes in Clinical Isolates of Bacillus anthracis by Multiplex PCR and DNA Probes

  • Kumar, Sanjay;Tuteja, Urmil
    • Journal of Microbiology and Biotechnology
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    • 제19권11호
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    • pp.1475-1481
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    • 2009
  • Anthrax is a zoonotic disease caused by Bacillus anthracis, and well recognized as a potential agent for bioterrorism. B. anthracis can be identified by detecting the virulence factors genes located on two plasmids, pXO1 and pXO2. The aim of the present study was to determine the presence of virulence genes in 27 isolates of B. anthracis isolated from clinical and environmental samples. For this purpose, multiplex PCR and DNA probes were designed to detect protective antigen (pag), edema factor (cya), lethal factor (lef), and capsule (cap) genes. Our results indicated that all the isolates contained all the above virulence genes, suggesting that the isolates were virulent. To the best our knowledge, this is the first study about the determination of virulence marker genes in clinical and environmental isolates of B. anthracis using multiplex PCR and DNA probes in India. We suggest that the above methods can be useful in specific identification of virulent B. anthracis in clinical and environmental samples.