• 제목/요약/키워드: Baby-Cell

검색결과 37건 처리시간 0.027초

An unusual de novo duplication 10p/deletion 10q syndrome: The first case in Korea

  • Lee, Bom-Yi;Park, Ju-Yeon;Lee, Yeon-Woo;Oh, Ah-Rum;Lee, Shin-Young;Choi, Eun-Young;Kim, Moon-Young;Ryu, Hyun-Mee;Park, So-Yeon
    • Journal of Genetic Medicine
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    • 제12권1호
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    • pp.49-56
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    • 2015
  • We herein report an analysis of a female baby with a de novo dup(10p)/del(10q) chromosomal aberration. A prenatal cytogenetic analysis was performed owing to abnormal ultrasound findings including a choroid plexus cyst, prominent cisterna magna, and a slightly medially displaced stomach. The fetal karyotype showed additional material attached to the terminal region of chromosome 10q. Parental karyotypes were both normal. At birth, the baby showed hypotonia, upslanting palpebral fissures, a nodular back mass, respiratory distress, neonatal jaundice and a suspicious polycystic kidney. We ascertained that the karyotype of the baby was 46,XX,der(10)($pter{\rightarrow}q26.3::p11.2{\rightarrow}pter$) by cytogenetic and molecular cytogenetic analyses including high resolution GTG-and RBG-banding, fluorescence in situ hybridization, comparative genomic hybridization, and short tandem repeat marker analyses. While almost all reported cases of 10p duplication originated from one of the parents with a pericentric inversion, our case is extraordinarily rare as the de novo dup(10p)/del(10q) presumably originated from a rearrangement at the premeiotic stage of the parental germ cell or from parental germline mosaicism.

Design, Synthesis and Biological Evaluation of Novel Analogs of Bortezomib

  • Rao, R. Janaki Rama;Rao, A.K.S. Bhujanga;Swapna, K.;Rani, B. Baby;Kumar, S. Prasanna;Awantika, S.;Murthy, Y.L.N.
    • 대한화학회지
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    • 제55권5호
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    • pp.765-775
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    • 2011
  • Novel analogs of bortezomib were designed, synthesized and in vitro biological evaluation was carried out using human tumor cell lines A549 and PC3. Docking studies of these analogs of bortezomib was discussed. According to biological investigations, the inhibitors 4, 6, and 8 were found to be more potent than reference drug candidate bortezomib. A549 cell line showed significant sensitivity towards 4, 6, and 8 with $IC_{50}$ values 14.03, 18.5, and 12.4 nM, respectively, and PC3 cell line showed IC50 values 26.1, 37.0, and 21.2 nM, respectively. The $IC_{50}$ values of bortezomib in these cell lines are 27.3 nM and 42.0 nM.

빈랑 추출물의 새로운 항바이러스 활성 (The Antiviral Effects of Areca catechu L. Extract)

  • 이도승;부경환;김영천;이진만;강승태;이왕식;류기중;이동선
    • 한국식품과학회지
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    • 제46권2호
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    • pp.245-248
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    • 2014
  • Newcastle disease virus (NDV) 감염된 baby hamster kidney (BHK) 세포에서 합포체 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase (HN)에 의해 일어난다. HAU 값은 추출물의 농도가 25.0과 $3.2{\mu}g/mL$ 사이에서는 현저하게 감소하였으나, NDV 감염된 HAD (%)는 $25.0{\mu}g/mL$ 농도에서 광범위한 흡착능의 감소를 나타내 바이러스 당단백질의 세포내 생합성은 저해되지 않았다. 그러므로, 약용식물인 빈랑 메탄올 추출물이 바이러스 당단백질의 세포막으로의 수송과 함께 합포체 형성을 저해하여 항바이러스 작용을 하였다. 또한 빈랑 추출물의 저해활성을 조사한 결과 ${\alpha}$-glucosidase에 대한 추출물의 $IC_{50}$$10.0{\mu}g/mL$이었으며, ${\alpha}$-mannosidase, ${\beta}$-glucosidase, ${\beta}$-mannosidase에 대한 빈랑 추출물의 $IC_{50}$은 각각 20.0, 40.0, $80.0{\mu}g/mL$로 나타나 ${\beta}$-type glycosidases 보다 ${\alpha}$-type glycosidases에 대한 효소활성 저해능이 우수하였다. 따라서 $IC_{50}$농도에서는 세포내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 판단되었으며 향후 항바이러스 관련 작용기작의 연구가 필요하다고 판단된다.

초두구 추출물의 α-glucosidase 활성 억제에 의한 항바이러스 활성 (Methanol Extract of Alpinia katsumadai Hayata has Anti-viral Activity by Inhibition of α-glucosidase)

  • 이도승;부경환;김영천;이진만;김성철;이왕식;류기중;이동선
    • Journal of Applied Biological Chemistry
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    • 제57권2호
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    • pp.179-182
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    • 2014
  • ${\alpha}$-glucosidase 활성을 저해하는 초두구 메탄올 추출물의 항바이러스 활성을 연구하였다. Newcastle disease virus (NDV) 감염된 baby hamster kidney (BHK) 세포에서 Syncytium (합포체) 형성은 세포막 표면으로의 수송된 바이러스 당단백질 hemagglutinin-neuramidase (HN)에 의해 일어난다. 초두구 추출물$IC_{50}$ $25{\mu}g/mL$) 처리된 세포에서 바이러스 당단백질의 수송과 함께 합포체 형성이 저해되었다. 또한 $IC_{50}$ 농도에서는 세포 내에서 당단백질 생합성은 저해되지 않으며 당단백질의 수송을 저해하는 것으로 추론된다.

단일 유전자 질환에 대한 착상전 유전진단 (Preimplantation Genetic Diagnosis for Single Gene Disorders)

  • 이형송;김민지;강인수
    • Journal of Genetic Medicine
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    • 제6권2호
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    • pp.131-145
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    • 2009
  • 착상전 유전진단은 유전질환이 이환될 가능성이 있는 부부들을 대상으로 산전진단을 통한 임신중절의 위험성 없이 정상적인 아이를 가질 수 있게 도와주는 보조생식술의 한 방법으로 확립되었다. 단일 할구를 대상으로 하는 분자생물학 및 분자생물학적 기술의 발전은 착상전 유전진단의 정확성을 높은 수준에 이르게 하였고 whole genome amplification 방법을 이용함으로써 단일세포로부터 여러 가지 다양한 진단을 동시에 수행 가능케 하였으며 단일 유전자 질환에 대한 착상전 유전진단에서의 오진을 감소시킬 수 있었다. 따라서 PCR을 이용한 단일 유전자 질환에 대한 착상전 유전진단의 적용가능 유전질환은 더욱 확대될 것이며 건강한 아이의 출산을 원하는 더 많은 부부들에게 기회를 제공해 줄 것이다. 본 종설에서는 현재 단일유전자 질환에 대한 착상전 유전진단을 시행하는 대부분의 센터에서 시행하고 있는 생검 방법과 multiplex PCR, PCR 후 진단 방법, 그리고 multiple displacement amplification 등의 분자생물학적 방법과 단일 세포 분석에서의 문제점 등을 포함한 단일 유전자 질환에 대한 착상전 유전진단 전반에 관하여 논의할 것이다.

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선천성 종격동 기형종(신생아에 발생한 미성숙 기형종) 치험 1례 보고 (Clinical Experience of Mediastinal Immature Teratoma in a Newborn)

  • 정승혁
    • Journal of Chest Surgery
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    • 제28권5호
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    • pp.530-532
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    • 1995
  • The teratomas seem to be the most common mediastinal tumor in Korea. However, the immature teratomas are the rarest type of these mediastinal teratomas. A case of immature teratoma of the mediastinum occurring in a newborn male is reported. He suffered from severe dyspnea since birth. Simple chest X-ray and chest sonogram revealed huge mass shadow in fight whole lung field. On 6th day after birth, the operation was done and the mass was completely excised. Biopsy showed elements of mature tissues derived from 3 germ cell layers including immature neuronal components. The baby is well without evidence of disease till now.

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Adenosine Induced Apoptosis in BHK Cells via P1 Receptors and Equilibrative Nucleoside Transporters

  • Sun, Wentian;Khoo, Hoon Eng;Tan, Chee Hong
    • BMB Reports
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    • 제38권3호
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    • pp.314-319
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    • 2005
  • Adenosine, as a ubiquitous metabolite, mediates many physiological functions via activation of plasma membrane receptors. Mechanisms of most of its physiological roles have been studied extensively, but research on adenosine-induced apoptosis (AIA) has only started recently. In this study we demonstrate that adenosine dose-dependently triggered apoptosis of cultured baby hamster kidney (BHK) cells. Adenosine-induced apoptotic cell death was characterized by DNA laddering, changes in nuclear chromatin morphology and phosphatidylserine staining. Apoptosis was also quantified by flow cytometry. Results suggest the involvement of adenosine $A_1$ and $A_3$ receptors as well as equilibrative nucleoside transporters in apoptosis induced by adenosine. These results indicate a receptor-transporter co-signaling mechanism in AIA in BHK cells. The involvement of $A_1$ and $A_3$ receptors also implies a possible apoptotic pathway mediated by G protein-coupled receptors.

Birth of a healthy infant after bone marrow-derived cell therapy

  • Patel, Nayana H;Jadeja, Yuvraj D;Patel, Niket H;Patel, Molina N;Bhadarka, Harsha K;Chudasama, Piyush N;Thakkar, Harmi R
    • Clinical and Experimental Reproductive Medicine
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    • 제48권3호
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    • pp.268-272
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    • 2021
  • Bone marrow-derived cell (BMDC) therapy has numerous applications as potential biological cells for use in regenerative medicine. Here, we present an original case of endometrial atrophy associated with genital tuberculosis in a woman who achieved a live birth with BMDC. This 27-year-old woman came to our center with endometrial atrophy and primary infertility. She had a past history of genital tuberculosis and amenorrhea. Her husband's semen quality was normal. The patient was counseled for hysteroscopy due to thin endometrium and advised in vitro fertilization (IVF) with donor eggs in lieu of poor ovarian reserve. Several attempts of IVF with hormone replacement therapy (HRT) were made, but the desired thickness of the endometrium was not achieved. Uterine artery injection of BMDC through interventional radiology was given, followed by HRT for three months, which resulted in improved endometrium. This was subsequently followed by IVF with donor egg. The treatment resulted in the conception and delivery of a 3.1-kg baby boy through lower segment caesarean section with no antenatal, intranatal or postnatal complications. Recently, there has been massive interest in stem cells as a novel treatment method for regenerative medicine, and more specifically for the regeneration of human endometrium disorders like Asherman syndrome and thin endometrium, which was the reason behind using this strategy for treatment.

Antiviral Activity of Methylelaiophylin, an ${\alpha}$-Glucosidase Inhibitor

  • Lee, Do-Seung;Woo, Jin-Kyu;Kim, Dong-Hern;Kim, Min-Young;Cho, So-Mi K.;Kim, Jae-Hoon;Park, Se-Pill;Lee, Hyo-Yeon;Riu, Key Zung;Lee, Dong-Sun
    • Journal of Microbiology and Biotechnology
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    • 제21권3호
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    • pp.263-266
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    • 2011
  • Methylelaiophylin isolated from Streptomyces melanosporofaciens was selected as an ${\alpha}$-glucosidase inhibitor with an $IC_{50}$ value of 10 ${\mu}M$. It showed mixed-type inhibition of ${\alpha}$-glucosidase with a $K_i$ value of 5.94 ${\mu}M$. In addition, methylelaiophylin inhibited the intracellular trafficking of hemagglutinin-neuramidase (HN), a glycoprotein of Newcastle disease virus (NDV), in baby hamster kidney (BHK) cells. Methylelaiophylin inhibited the cell surface expression of NDV-HN glycoprotein without significantly affecting HN glycoprotein synthesis in NDV-infected BHK cells.

FRT차량의 사용자중심적인 실내디자인 연구 (Research of the user oriented interior design for FRT)

  • 김상중;김성남
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2004년도 추계학술대회 논문집
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    • pp.455-460
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    • 2004
  • The Fuel cell Rubber tired Train (FRT), which is now getting the attention as the next generation vehicle with its environment-friendliness, is the transportation for smooth connections of city traffic. It is the revival of the surface-car system with its revaluation of the function and technological development. Accordingly, fixed time operation and high speed driving became possible. FRT is operated together with other vehicles on the regular drive way. While this vehicle can solve the problem of traffic congestion in the urban area, it also can be cost-effective when it is compared to the cost of subway construction. It is also designed to minimize the underground or elevated traffic lane, to introduce the new construction technology, to reduce a term of works, and to cut down the operation cost by unmanned automatic driving system. Furthermore, it is considered as the alternative measure of other transportation due to its potential for the ecological way of speed improvement and the accessability to the disabled, elderly and children by developing the vehicle with folding steps or by building the high boarding platforms. In this research, I concentrated on the user oriented interior design of the FRT to make it passenger-friendly and safe in order to maximize the utilization of the vehicle so that all users including wheelchaired, user with baby carriage, elderly and children can conveniently use this vehicle.

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