• Journal of Life Science
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• v.33 no.9
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• pp.713-723
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• 2023

Lung cancer is a type of cancer that has the highest mortality rate. It is mainly classified into small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Chemotherapy is used to treat lung cancer, but long-term treatment causes side effects and drug resistances. Curcumin is a bright yellow polyphenol extracted from the root of turmeric. It has biological activities, such as anti-oxidant, anti-cancer, and anti-inflammatory effects. In this study, we observed differential cell death in human lung cancer cells. Based on the results, curcumin at 10, 30, and 50 μM exhibited a dose-dependent inhibition on the cell survival of several lung cancer cells, with minor differential phenotypes. In addition, apoptosis, autophagy, and reactive oxygen species (ROS) regeneration were observed through flow cytometry. Curcumin dose-dependently increased these phenotypes in A549 (NSCLC) and DMS53 (SCLC), which were restored by corresponding inhibitors. Western blotting was performed to measure the level of expression of apoptosis- and autophagy-related proteins. The results indicate that Bax, PARP, pro-caspase-3, and Bcl-2 were dose-dependently regulated by curcumin, with seemingly higher Bax/Bcl-2 ratios in DMS53. In addition, autophagic proteins, p-AKT, p62, and LC3B, were dose-dependently regulated by curcumin. ROS inhibition by diphenyleneiodonium reduced the induction of apoptosis and autophagy generated by curcumin. Taken together, it is suggested that curcumin induces apoptosis and autophagy via ROS generation, leading to cell death, with minor differences between human lung cancer cells.

• The Korea Journal of Herbology
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• v.24 no.1
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• pp.59-71
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• 2009

Objectives：The present study has been undertaken to investigate the effects of Mantidis $O\ddot{O}theca$ and Mori Fructus on treatment of osteoporosis in ovariectomized rats. Methods： In this experiment, the rats were ovariectomized. Rats were administered by Mantidis $O\ddot{O}theca$ and Mori Fructus. The levels of bone mineral density, osteocalcin.ALP.calcium.phosphorus in serum, calcium. phosphorus.deoxypyridinoline in urine and calcium.phosphorus.ash weight in bone were measured. Results： 1. The levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ group. And the levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 8 weeks in Mori Fructus group. 2. The levels of serum osteoclacin and ALP showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. The levels of serum calcium showed significant decrease in comparison with OVX group at 4 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. The levels of serum phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. 3. The levels of urine calcium, phosphorus and deoxypyridinoline showed significant decrease in comparison with OVX group in Mantidis $O\ddot{O}theca$ and Mori Fructus group. 4. The levels of fibula-tibial calcium and phosphorus showed significant increase in comparison with OVX group in Mantidis $O\ddot{O}theca$ group and Mori Fructus group. The levels of femoral calcium and phosphorus showed significant increase in comparison with OVX group in Mori Fructus group. The levels of femoral and fibula-tibial ash weight showed significant increase in comparison with OVX group in Mantidis $O\ddot{O}theca$ group and Mori Fructus group. Conclusions： Reviewing these experimetal results, it appeared that Mantidis $O\ddot{O}theca$ and Mori Fructus had efficacy on treatment of osteoporosis.

• Journal of the Korean Geotechnical Society
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• v.24 no.4
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• pp.79-88
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• 2008

Recently, the high speed railway comes into the spotlight as the important and convenient traffic infrastructure. In Korea, Kyung-Bu high speed train service began in bout 400 km section in 2004, and the Ho-Nam high speed railway will be constructed by 2017. The high speed train will run with a design maximum speed of 300-350 km/hr. Since the trains are operated at high speed, the differential settlement of subgrade under the rail is able to cause a fatal disaster. Therefore, the differential settlement of the embankment must be controlled with the greatest care. Furthermore, the characteristics and causes of settlements which occurred under construction and post-construction should be investigated. A considerable number of studies have been conducted on the settlement of the natural ground over the past several decades. But little attention has been given to the compression settlement of the embankment. The long-term settlement of compacted fills embankments is greatly influenced by the post-construction wetting. This is called 'hydro collapse' or 'wetting collapse'. In spite of little study for this wetting collapse problem, it has been recognized that the compressibility of compacted sands, gravels and rockfills exhibit low compressibility at low pressures, but there can be significant compression at high pressures due to grain crushing (Marachi et al. 1969, Nobari and Duncan 1972, Noorany et al. 1994, Houston et al. 1993, Wu 2004). The characteristics of compression of fill materials depend on a number of factors such as soil/rock type, as-compacted moisture, density, stress level and wetting condition. Because of the complexity of these factors, it is not easy to predict quantitatively the amount of compression without extensive tests. Therefore, in this research I carried out the wetting collapse tests, focusing on various soil/rock type, stress levels, wetting condition more closely.

• Journal of the Korean Orthopaedic Association
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• v.52 no.1
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• pp.1-6
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• 2017

Purpose: To retrospectively compare minimally invasive percutaneous plate osteosynthesis (MIPPO) with open plate fixation for the treatment of clavicle midshaft fracture. Materials and Methods: Between November 2011 to May 2014, 40 cases that were followed for more than 1 year-among all cases of MIPPO and open plate fixation-were analyzed. The study population was divided into two groups: group A included 20 cases of MIPPO and group B included 20 open plate fixation cases. The comparative analysis between the two groups was based on the operative time, bone union, functional evaluation (American Shoulder and Elbow Society score), incision length, pain relief (visual analogue scale, VAS), and complication. Results: The bone union was successful for all the cases, and the functional evaluation scores of the shoulder joint were satisfactory for both groups (p>0.05). The operative time was 47.5 minutes and 58.7 minutes for group A and B, respectively (p<0.05). The incision length for group A was 6.2 cm and that for group B was 10.7 cm with statistical significance (p<0.05). Pain relief (VAS) after surgery for group A showed a quick recuperation in the early stages. For complications, there were 2 cases of dysesthesia and 1 case of malunion due to metal fixation failure in group A. There were 6 cases of dysesthesia and 2 cases of cosmetic problem due to hypertrophic scar in group B. Conclusion: Surgical results of clavicle shaft fracture were satisfactory for both groups, but group A had advantages of shorter operative time, minimal incision length, and better pain relief in the early stages compared with group B. However, we need further evaluations with long-term follow-up results and complications, such as malunion and exposure on radiation.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.1D
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• pp.25-32
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• 2009

Currently, various types of TDM (Transportation Demand Management) policies are being studied and implemented in an attempt to overcome the limitations of supply oriented policies. In this context, this paper addressed issues of effectiveness and possible domestic implementation of the HOT lane. The possible site of implementation selected for this simulation study is part of the Kyung-bu freeway, where a dedicated bus lane is currently being operated. Minimum length of distance required in between interchanges and access points of the HOT lane for vehicles to safely enter and exit the lane, and traffic management policies for effectively managing the weaving traffic trying to enter and exit the HOT lane were presented. A 5.2km section of freeway from Ki-heuing IC to Suwon IC and a 8.3km section from Hak-uei JC to Pan-gyo JC have been selected as possible sites of implementation for the HOT lane, in which congestion occurs regularly due to the high level of travel demand. VISSIM simulation program has been used to analyze the effects of the HOT lane under the assumption that one-lane HOT lane has been put into operation in these sections and that the lane change rate were in between 5% to 30%. The results of each possible scenario have proven that overall travel speed on the general lanes have increased as well by 1.57~2.62km/h after the implementation of the HOT lane. It is meaningful that this study could serve as a basic reference data for possible follow-up studies on the HOT lane as one effective method of TDM policies. Considering that the bus travel rate would continue increase and assuming the improvement in travel speed on general lanes, similar case study can be implemented where gaps between buses on bus lane are available, as a possible alternative of efficient bus lane management policies.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.7
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• pp.902-909
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• 2023

This study evaluated the performance test of a small-sized LTE-Maritime(LTE-M) transceiver that was developed and promoted to expand the use of intelligent maritime traf ic information services led by the Ministry of Oceans and Fisheries with the aim of supporting the prevention of maritime accidents. Accoriding to statistics, approximately 30% of all marine accidents in Korean water occur with ships weighing less than 3 tons. Therefore, the blind spots of maritime safety must be supplemented through the development of small-sized transceivers. The small transceiver may be used in fishing boats that are active near coastal waters and in water leisure equipment near the coastline. Therefore, verifying whether sufficient performance and stable communication quality are provided is necessary, considering the environment of their real usage. In this study, we reviewed the communication quality goals of the LTE-M network and the performance requirements of small-sized transceivers suggested by the Ministry of Oceans and Fisheries, and proposed a test plan to appropriately evaluate the performance of small-sized transceivers. The validity of the proposed test method was verified for six real-sea areas with a high frequency of marine accidents. Consequently, the downlink and uplink transmission speeds of the small-sized LTE-M transceiver showed performances of 9 Mbps or more and 3 Mbps or more, respectively. In addition, using the coverage analysis system, coverage of more than 95% and 100% were confirmed in the intensive management zone (0-30 km) and interesting zone (30-50 km), respectively. The performance evaluation method and test results proposed in this paper are expected to be used as reference materials for verifying the performance of transceivers, contributing to the spread of government-promoted e-navigation services and small-sized transceivers.

• Journal of the Society of Disaster Information
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• v.19 no.4
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• pp.976-983
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• 2023

Purpose: The dynamic behavior of a bridge structure under seismic loading depends on many uncertainties, such as the nature of the seismic waves and the material and geometric properties. However, not all uncertainties have a significant impact on the dynamic behavior of a bridge structure. Since probabilistic seismic performance evaluation considering even low-impact uncertainties is computationally expensive, the uncertainties should be identified by considering their impact on the dynamic behavior of the bridge. Therefore, in this study, a global sensitivity analysis was performed to identify the main parameters affecting the dynamic behavior of bridges with I-curved girders. Method: Considering the uncertainty of the earthquake and the material and geometric uncertainty of the curved bridge, a finite element analysis was performed, and a surrogate model was developed based on the analysis results. The surrogate model was evaluated using performance metrics such as coefficient of determination, and finally, a global sensitivity analysis based on the surrogate model was performed. Result: The uncertainty factors that have the greatest influence on the stress response of the I-curved girder under seismic loading are the peak ground acceleration (PGA), the height of the bridge (h), and the yield stress of the steel (fy). The main effect sensitivity indices of PGA, h, and fy were found to be 0.7096, 0.0839, and 0.0352, respectively, and the total sensitivity indices were found to be 0.9459, 0.1297, and 0.0678, respectively. Conclusion: The stress response of the I-shaped curved girder is dominated by the uncertainty of the input motions and is strongly influenced by the interaction effect between each uncertainty factor. Therefore, additional sensitivity analysis of the uncertainty of the input motions, such as the number of input motions and the intensity measure(IM), and a global sensitivity analysis considering the structural uncertainty, such as the number and curvature of the curved girders, are required.

• Journal of Life Science
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• v.34 no.7
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• pp.500-508
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• 2024

Recently, there has been increasing interest in enhancing the indoor air quality, particularly in response to the growing utilization of public facilities. The focus of this study was on assessing the efficacy and safety of an air sterilizer equipped with electrolytic salt catalysts. To that end, we evaluated the antimicrobial activity of the vapor spraying from the air sterilizer and its cytotoxicity in condensed form on human cell lines (HaCaT, BEAS-2B, and THP-1). Against the test organisms, which comprised five bacterial strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium) and one fungal strain (Candida albicans), the air sterilizer exhibited relatively high antimicrobial activities ranging from 10.89 to 73.98% following 1 and 3 hr of vapor spraying, which were notably time-dependent. Importantly, cytotoxicity assessments on human cells indicated no significant harmful effect even at a 1.0% concentration. Comprehensive safety evaluations included morphological observations, gene expression (Bcl-2, Bax) tests, and FACS analysis of intracellular ROS levels. Consistent with previous cytotoxicity findings, these estimates demonstrated no significant changes, highlighting the air sterilizer's safety and antimicrobial activities. In a simulated 20-hr operation within an indoor environment, the air sterilizer not only showed an 89.4% removal of total bacteria but also a 100.0% removal of Escherichia sp. and fungi. This research outlines the potential of the developed electrolytic salt catalyst air sterilizer to effectively remove indoor microbial pollutants without compromising human safety, underscoring the solution that it offers for improving indoor air quality.

• Journal of Food Hygiene and Safety
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• v.39 no.1
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• pp.54-60
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• 2024

This study investigated the effectiveness of using pathogens and aqueous acids to reduce the Aspergillus ochraceus and Rhodotorula mucilaginosa contamination in livestock production environments. For this study, 1 mL of each bacterial suspension (10⁷-10⁸ spores/mL) was inoculated on a knife surface, dried at 37℃, and used under each treatment condition. First, to investigate the effect of organic acids, acetic, lactic, and citric acids were used. Subsequently, to select the appropriate concentration, they were prepared at concentrations of 0.5, 1, 2, 3, 4, and 5%, respectively. Accordingly, to further maximize the effect of organic acid treatment, we combined the treatment with ultraviolet light. The two strains showed a significant difference (P<0.05) compared to the initial strain, with a greater than 90% decrease in the concentrations of all organic acids. Consequently, acetic and lactic acids decreased by approximately 5 and 2 log colony forming unit (CFU)/cm², respectively, when treated with ultraviolet light (360 mJ/cm²); however, citric acid decreased by less than 1 log CFU/cm². However, when manufactured with 4% acetic acid, a severe malodor was emitted, making it difficult for workers to use it in a production environment. Accordingly, the optimal treatment conditions for organic acid and ultraviolet light for application were selected as follows: immersion in a 4% lactic acid solution for 1 minute and then, sterilization with ultraviolet light at 360 mJ/cm². Finally, when a pork meat sample was cut with a knife that was finally washed with lactic acid and treated with ultraviolet light, the low level of inoculum transferred from the cleaned knife to the surface of the sample was not detected. In conclusion, using this established method can prevent cross-contamination of the surface of the meat during processing.

• Journal of The Korean Society of Grassland and Forage Science
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• v.44 no.2
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• pp.99-105
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• 2024

This study was conducted to estimate the effects of the forage process on rumen fermentation characteristics and greenhouse gas emissions of rye. Rye was grown at the Taeyoung Livestock farm and harvested at the heading stage. The harvested rye (5 kg) was sub-sampled for fresh forage, hay, and silage in triplicates. The sub-sampled rye was freeze-dried or air-dried for fresh forage or rye hay, respectively. For rye silage, the sub-sampled rye forage was ensiled into a 10 L mini bucket silo and stored for 90 days. For 72 h rumen incubation, each forage (0.3 g) was placed into the incubation bottle with the rumen mixture (30 mL) in quadruplicates. After the incubation, total gas was measured and sub-sampled for CO₂ and CH₄ analyses, and the bottle content was centrifuged for in vitro digestibilities of dry matter (IVDMD) and neutral detergent fiber (IVNDFD), and rumen fermentation characteristics. Silage had higher crude protein, crude ash, and acid detergent fiber concentrations than fresh forage and hay but lower non-fiber carbohydrates and relative feed value (p<0.05). And, silage had higher lactic acid bacteria than the other forages but lower pH (p<0.05). After 72 h incubation in the rumen, fresh forage had higher IVDMD and butyrate content than the other forages (p<0.05). However, silage had higher rumen pH and propionate content than the other forages but lower A:P ratio (p<0.05). Regarding greenhouse gases, silage had lowest total gas (mL/g DMD and NDFD) and CH₄ (mL/g DMD and NDFD) emissions, while fresh forage had lowest CO₂ (mL/g DMD) emission (p<0.05). Therefore, this study concluded that the ensiling process of rye can effectively mitigate greenhouse gas emissions of Hanwoo.