• Title/Summary/Keyword: BTS test

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Discovery of Candidate SNP Related to Meat Quality Using the BcSNPdb and Cattle QTLdb in Hanwoo (한우에서 Cattle QTLdb와 BcSNPdb를 이용한 육질연관 후보 SNP 발굴)

  • Kim, Dae-Hyun;Lee, Yoon-Seok;Oh, Young-Sook;Choi, Chang-Bon;Yeo, Jung-Sou
    • Journal of Animal Science and Technology
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    • v.50 no.6
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    • pp.775-782
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    • 2008
  • This study was aimed to find SNPs related with marbling score in Hanwoo(Korean cattle) using BcSNPdb. This study was searched QTL region related with marbling score and extracted 3,605 SNPs by applying BcSNPdb. Among these SNPs, 347 nonsynonymous SNP were selected and 160 SNPs were verified by PCR and finally proven with application to experimental data of the national progeny test. BTS_003888, BTS_012665 and BTS_009454 candidate SNPs were revealed significantly associated with marbling score(P<0.05), and BTS_025951 candidate SNPs was significantly associated with backfat thickness(P<0.05). From the result, SNPs from BTS_003888, BTS_009454, BTS_052584 and BTS_025951 were considered to be useful for the advancement in selective improved model in marbling score and backfat thickness of Hanwoo.

Evaluation of Boar Sperm Viability by MTT Reduction Assay in Beltsville Thawing Solution Extender

  • Byuna, J.W.;Choo, S.H.;Kim, H.H.;Kim, Y.J.;Hwang, Y.J.;Kim, D.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.4
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    • pp.494-498
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    • 2008
  • MTT (3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) reduction assay is a method that validates the viability of an active cell. Dehydrogenase in mitochondria converts yellow colored insoluble tetrazolium salt to purple colored water-soluble formazan. Sperm also have mitochondria in the midpiece, therefore sperm viability could be evaluated by MTT reduction assay. Several studies have already demonstrated the capability of application of the MTT reduction assay to sperm of several species in Hepes-BSA buffer. Because most liquid semen was diluted in extender like BTS (Beltsville Thawing Solution), Modena or Androhep when it is used or transferred, semen needed another dilution in Hepes-BSA buffer to assess sperm viability. In this study, we evaluated boar sperm viability especially in BTS extended semen and compared the efficiency of this test with eosin-nigrosin staining. We used the fresh BTS extended semen from a local A.I center. Semen sample was diluted to $3.0{\times}10^7$ sperms/ml in BTS. The rates of formazan production were measured in 96-well microtiter plates immediately and 1h after incubation at $17^{\circ}C$ using a spectrophotometer at wave length 560 nm. Simultaneously, split samples of the same semen were tested, using eosin-nigrosin staining to compare the efficiency of the MTT assay of sperm viability in BTS. The correlation between the results of these tests was calculated using Student-t test and ANOVA. The results revealed a strong correlation between the results of MTT reduction rate and the results that were simultaneously determined by eosin-nigrosin staining at 1 h. In conclusion, the MTT reduction test was an effective and simple method to validate sperm viability and it could be used as a simple tool to evaluate sperm viability in the local A.I center and laboratory.

Effects of Using a Mobile Phone on Postural Control (휴대전화 이용이 자세조절에 미치는 영향)

  • Won, Jong-Im
    • Physical Therapy Korea
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    • v.19 no.3
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    • pp.61-71
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    • 2012
  • In daily activities, people often perform two or more tasks simultaneously. This is referred to as dual-tasking or multi-tasking. The purpose of this study was to examine the effects of performing dual tasks while using a mobile phone on static and dynamic postural stability. Twenty-four subjects were asked to stand on a force plate and then instructed to perform a balance task only (BT), a balance task while listening to music (BTL), a balance task while talking on the mobile phone (BTT), and a balance task while sending text messages (BTS). We used the BioRescue$^{(R)}$ to measure postural sway and limit of stability for static and dynamic postural stability. Also the star excursion balance test (SEBT) was used to measure dynamic postural stability. A one-way ANOVA with repeated measures was used to compare the effects of the BT, BTL, BTT, and BTS. The Bonferroni's post hoc test was used to determine the differences among four tasks. Carrying out the BTS significantly decreased the limit of stability compared with carrying out the BT, BTL, and BTT (p<.05). In limit of stability, total surface area of BTT was more significantly decreased than that of BT and total surface area of BTS was more decreased than that of BT, BTL and BTT (p<.05). In the SEBT, the BTS displayed significantly smaller reach distance values compared with the BT or BTL (p<.05). These findings suggest that performing the balance task while sending text message on the mobile phone decreases dynamic postural stability, whereas performing the same task while listening to music using the mobile phone does not. Therefore, it requires more attention to maintain dynamic balance while sending text messages.

EMI database analysis focusing on relationship between density and mechanical properties of sedimentary rocks

  • Burkhardt, Michael;Kim, Eunhye;Nelson, Priscilla P.
    • Geomechanics and Engineering
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    • v.14 no.5
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    • pp.491-498
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    • 2018
  • The Earth Mechanics Institute (EMI) was established at the Colorado School of Mines (CSM) in 1974 to develop innovations in rock mechanics research and education. During the last four decades, extensive rock mechanics research has been conducted at the EMI. Results from uniaxial compressive strength (UCS), Brazilian tensile strength (BTS), point load index (PLI), punch penetration (PP), and many other types of tests have been recorded in a database that has been unexamined for research purposes. The EMI database includes over 20,000 tests from over 1,000 different projects including mining and underground construction, and analysis of this database to identify relationships has been started with preliminary results reported here. Overall, statistically significant correlations are identified between bulk density and mechanical strength properties through UCS, BTS, PLI, and PP testing of sedimentary, igneous, and metamorphic rocks. In this paper, bulk density is considered as a surrogate metric that reflects both mineralogy and porosity. From this analysis, sedimentary rocks show the strongest correlation between the UCS and bulk density, whereas metamorphic rocks exhibit the strongest correlation between UCS and PP. Data trends in the EMI database also reveal a linear relationship between UCS and BTS tests. For the singular case of rock coral, the database permits correlations between bulk density of the core versus the deposition depth and porosity. The EMI database will continue under analysis, and will provide additional insightful and comprehensive understanding of the variation and predictability of rock mechanical strength properties and density. This knowledge will contribute significantly toward the increasingly safe and cost-effective geostructures and construction.

The Effect of Side-Step Tasks Based Circular Training Program on Balance and Gait in Stroke Patients

  • Sang Jun Son;Joong-Hwi Kim
    • Physical Therapy Rehabilitation Science
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    • v.11 no.4
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    • pp.384-390
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    • 2022
  • Objective: The purpose of this study was to investigate the effect of the side-step tasks based circular training program (STCT) on balance and gait characteristics in stroke patients. Design: A randomized controlled trial Methods: Twenty-four stroke patients were randomly divided into two groups of twelve patients each. One group was applied with the STCT whereas the other group was treated with conservative physiotherapy (CP). The ability of gait was measured in 10m walking test and stride length on both side using BTS G-WALK (BTS Bioengineering S.p.A, Italy) and the ability of balance was measured in Berg Balance Scale (BBS) and Timed Up and Go Test (TUG). Results: The STCT group was significant differences in the balance parameters of BBS and TUG (p<0.05) and showed significant differences in gait variables in 10m walking speed, stride length of affected and non-affected side after the experiment before and after the experiment (p<0.05). In addition, the STCT group showed a significant difference in BBS compared to the control group (p<0.05). Conclusions: The results of this study confirmed that the side-step tasks based circular training program (STCT) improves balance and walking ability in stroke patients. STCT is expected to be used as a useful intervention method for stroke rehabilitation.

Chemical Contamination and Toxicity of Sediments from the Gunsan Coast, Korea

  • Lee, Wan-Seok;Choi, Minkyu;Hwang, Dong-Woon;Lee, In-Seok;Kim, Sook Yang
    • Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.241-250
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    • 2012
  • Polycyclic aromatic hydrocarbons (PAHs), butyltins (BTs), nonylphenol (NP), and fecal sterols concentrations in sediments were investigated from Gunsan coast of Korea to evaluate organic pollution from anthropogenic activities. Sediment toxicity was also examined by bacterial bioluminescence toxicity test (Vibrio fischeri). The concentrations of 16 PAHs in sediments ranged from 67.9 to 425 ng/g dry wt; BTs ranged from 2.79 to 14.1 ng Sn/g dry wt; NP ranged from 20.7 to 2171 ng/g dry wt; and coprostanol, a fecal sterol, ranged from 7.60 to 245 ng/g dry wt. Effective concentration 50% ($EC_{50}$) of sediments ranged from 0.38 to 23.8 mg/mL. Most of the chemicals were present at levels lower than or comparable to the previously reported values from Korea. However, NP levels in the present study were in the high range of levels reported from the Korean coast, and 40% of the measured samples exceeded screening and ecotoxicological values of NP suggested by the Netherlands and Canada. This suggests that an ongoing source of NP is a serious concern in the Gunsan coast. High levels of contaminants were found in the proximity of potential sources, such as the outfall of a wastewater treatment plant for NP, an anthracite-fired power plant for PAHs, and ports for BTs, fecal sterols, and sediment toxicity. This indicates that Gunsan coast has various potential sources of marine sediment contaminants.

Investigating the effect of strength on the LCPC abrasivity of igneous rocks

  • Kahraman, Sair;Fener, Mustafa;Kasling, Heiko;Thuro, Kurosch
    • Geomechanics and Engineering
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    • v.15 no.2
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    • pp.805-810
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    • 2018
  • The abrasivity of rocks results in tool wear in rock excavation or drilling projects. It can affect significantly the cost and schedule of the projects performed in abrasive rock massess. For this reason, the understanding of the mechanism of rock abrasivity is very important for excavation projects. This study investigates the effect of strength on the LCPC abrasivity coefficient (LAC) for igneous rocks. The LCPT test, the uniaxial compressive strength (UCS) and the Brazilian tensile strength (BTS) tests were carried out on the igneous rock samples. The abrasive mineral content (AMC) was also determined for each rock type. First, the LAC was correlated to the AMC and a very good correlation was found between the two parameters. Then, the multiple regression analysis was carried out by including the AMC, UCS and BTS to the analysis in order to infer the effect of the strength on the LAC. It was seen that the correlation coefficients of multiple regression models were greater than that of the relation between the LAC and the AMC. It is concluded that the AMC is the dominant parameter determining the abrasivity of rock. On the other hand, the rock strength has also significant effect on rock abrasivity.

Characterization of Electrical Properties on Cu Diffusion in Low-k Dielectric Materials for ULSI Interconnect (반도체 배선용 저 유전 물질에서의 구리 확산에 대한 전기적 신뢰성 평가)

  • Lee Hee-Chan;Joo Young-Chang;Ro Hyun-Wook;Yoon Do-Young;Lee Jin-kyu;Char Kook-Heon
    • Journal of the Microelectronics and Packaging Society
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    • v.11 no.3 s.32
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    • pp.9-15
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    • 2004
  • We investigated the electrical properties of copolymer low-k materials that are compromised of the PMSSQ(Poly Methyl Silsesquioxane)-based matrix with the BTMSE (Bis Tri Methoxy Silyl Ethane) additives. We manufactured MIS-type test samples using the copolymer as the insulator and measured their leakage current and failure time by means of the BTS (bias-temperature-stress) test. The failure time was observed to decrease drastically when the porosity of the copolymer was increased over $30\%$. From the measurement of failure time with respect to temperature. the activation energy of Cu drift through the copolymer was calculated to be 1.51 eV.

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Electrical and Mechanical Properties of Cu(Mg) Film for ULSI Interconnect (고집적 반도체 배선용 Cu(Mg) 박막의 전기적, 기계적 특성 평가)

  • 안재수;안정욱;주영창;이제훈
    • Journal of the Microelectronics and Packaging Society
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    • v.10 no.3
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    • pp.89-98
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    • 2003
  • The electrical and mechanical properties of sputtered Cu(Mg) films are investigated for highly reliable interconnects. The roughness, adhesion, hardness and resistance to thermal stress of Cu(Mg) film annealed in vacuum at $400^{\circ}C$ for 30min were improved than those of pure Cu film. Moreover, the flat band voltage(V$_{F}$ ) shift in the Capacitance-Voltage(C-V) curve upon bias temperature stressing(BTS) was not observed and leakage currents of Cu(Mg) into $SiO_2$ were three times less than those of pure Cu. Because Mg was easy to react with oxide than Cu and Si after annealing, the Mg Oxide which formed at surface and interface served as a passivation layer as well.

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