• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.326-332
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• 2008

This study was conducted to evaluate an early-maturing rice cultivars adaptable to early transplanting cultivation in Yeongnam plain area. High temperature during the ripening stage of paddy rice under the early-transplanting cultivation in Yeongnam plain was to influence in several agronomic traits such as number of spikelet, ripening ratio, grain appearance of milled rice, and yield. 'Gounbyeo' and 'Junghwabyeo' were better than the others in grain appearance of milled rice and ripening ratio, but their yield potential were lower than that of the other varieties. Moreover, 'Gounbyeo' and 'Junghwabyeo' showed high ratio of broken rice. 'Unkwangbyeo' was the highest in yield and eating quality among the tested varieties, but also high percentage of white core and belly in milled rice. Therefore, in order to recommend those cultivars to farmers, it should be needed to establish an optimal cultivation method, such as transplanting density, fertilizer application, and harvesting time, etc. consequently, it should also be required to develop a new varieties with high ripening capability under high temperature condition.

• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.204-212
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• 2017

There is a growing preference for using doubled haploids (DHs) in maize breeding programs because they reduce the time required to generate and evaluate new lines to 2 years or less. However, there is an urgent need for efficient techniques that accurately discriminate between haploid and diploid maize kernels. Here, we investigate the effects of several hormones and chemicals on the germination of haploid and diploid maize kernels, including auxin, cytokinin, ethylene, abscisic acid (ABA) biosynthesis inhibitor (fluridone), ABA catabolism inhibitor (diniconazole), methyl jasmonate (MeJA), and NaCl. Ethylene effectively stimulated the germination of both haploid and diploid maize kernels. The ABA biosynthesis inhibitor fluridone, the ABA catabolism inhibitor diniconazole, and MeJA selectively stimulated the germination of haploid maize kernels. By contrast, gibberellin, 1-naphthaleneacetic acid (NAA), kinetin, and NaCl inhibited the germination of both haploid and diploid maize kernels. These results indicate that the germination of haploid maize kernels is selectively stimulated by fluridone and diniconazole, and suggest that ABA-mediated germination of haploid maize kernels differs from that of diploid maize kernels and other plant seeds.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.67-67
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• 2017

We developed the breeding materials with diverse grain size and shape in japonica rice. Grain size and shape are important factors affecting consumer preference and choice. However, most of Korean japonica rice cultivars have small, short, and round grain. To diversify the grain size and shape of japonica rice, we conducted the breeding program using donor parents, Jizi1560 and Jizi1581. Jizi1560 and Jizi1581 are japonica germplasm with extremely large grain. Four crosses between the each donor parents and high yielding japonica rice cultivars, Deuraechan and Boramchan, were constructed and then anther culture method was applied. We obtained 290 doubled-haploid (DH) lines with appropriated morphological traits and selected 91 DH lines with diverse grain size and shape. The grain related-traits of the selected DH lines showed a higher diversity when compared with 319 cultivars developed by NICS (264 japonica, 13 black, and 32 Tongil type cultivars). We designated the selected DH lines, four parents, and Daeripbyeo 1, large grain japonica cultivar, as the breeding materials for further analysis. The breeding materials were classified into five groups, A to E, based on the grain-related traits. Group A (including Jizi1581) and Group B (including Daeripbyeo 1) showed similar grain width, whereas Group A exhibited longer grain length and heavier grain weight. Group C (including Deuraechan and Boramchan) showed shorter and rounder grain shape and smaller grain size than any other groups. Group D including solely Jizi1560 had extremely large grain, such as the longest grain length, width, and thickness and heaviest grain weight. Group E including only two DH lines had long and slender grain shape, so that showed the highest ratio of length to width. The grain size and shape of the breeding materials exhibited beyond the characteristics of previously developed Korean japonica cultivars. The breeding materials will be applied in the breeding programs to diversify the grain size and shape of japonica rice.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.205-205
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• 2022

Sorghum (Sorghum bicolor L.) is a major cereal grain crop of the world and It can be cultivated under high temperature and dry condition with high adaptability to the adverse environment. It is usually eaten with rice in korea and most of domestic sorghum cultivars have been developed by pure line selection and cross breeding. In prior studies, Hybrid cultivar (F1 seed) was known to improve 30-40% in yield and resist to disease and pest than pure line varieties due to hybrid vigor. Therefore, study on the hybrid sorghum is continuously necessary. In korea, hybrid sorghum cultivar have been developed, so we need to find methods seed production technology for supply and commercialization of hybrid seed cultivar. Female inbred line(A-line) and male inbred line(R-line) are needed for hybrid seed production. This study was carried out to investigate growth characteristics and yield of hybrid sorghum according to the seedling period and planting rate between female inbred line(YSA1) and male inbred line (Sodamchal, Miryang 19). When the flowering period of two inbred groups in the process of hybrid seed production is coincides, hybrid seed production is increase. The female inbred line and male inbred lines were cultivated at Daegu in 2021. Two inbred lines were sown at intervals of 3 days and 7days and were evaluated flowering period. As a result, the flowering period of the female inbred line and male inbred lines were matched at the 7days interval. To find out optimal planting rate, two inbred lines were cultivated under different plating rate (4:2, 5:2, 6:2). Yield of YSA1/Sodamchal(F1) was the highest 130(kg/10a) in the 4:2 ratio and yield of YSA1/Miryang 19 was the highest quantity of 139(kg/10a) in the 6:2 ratio. The results of this study could be helpful for hybrid Sorghum seeds production.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.316-316
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• 2022

Azuki bean is the important bean crop in Korea, and is traditionally used as a filling material for red bean porridge, rice cakes, and bread, as well as for sediment. So far, the National Insititute of Crop Science has developed azuki bean varieties for various uses, such as for sediment, tea, and azuki bean sprouts. Among azuki bean elite lines, 25 strains were used to analyze the correlation between red bean quality characteristics and sediment yield. The crude protein of red beans was 25.0-28.9%, ash content was 3.8-5.3%, and fat was 0.4-1.0%. As for the appearance quality characteristics of azuki bean, one hundred seed weight was 11.1-19.5 g, the length of seed was 6.45-8.49 mm, the width seed was 4.84-6.45 mm, and the withd/lenght ratio was 0.72-0.89. When the azuki bean were boiled, the thickness of the seed coat was 0.14-0.27 mm and the length ratio of seed was 0.65-0.76 mm, showing that the length of seed was slightly larger than the width. The yield of azuki bean sediment was 239-284% for whole red bean paste, and 144-203% for fine azuki bean paste. As a result of analyzing the correlation between the quality characteristics of azuki beans, such as protein, husk and seed weight, and the yield of azuki bean sediment, the yield of whole azuki bean sediment showed a negative correlation with the seed coat thickness and the length ratio of the original grain at -0.62 and -0.45, respectively. The yield of fine sediment showed a negative correlation with the length ratio of whole azuki beans at -0.49, and a positive correlation with that of white beans at 0.41. However, protein and ash content did not show any correlation with sediment yield. In view of the above results, it is thought that it would be better to have a high seed weight or a low width/length ratio of seed to develope azuki bean cultivar for sediment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.2
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• pp.109-127
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• 2014

Maize is one of the most important food and feed crops in the world including Southeast Asia. In spite of numberous efforts with conventional breeding, the maize productions remain low and the loss of yields by drought and downy mildew are still severe in Asia. Genetic improvement of maize has been performed with molecular marker and genetic engineering. Because maize is one of the most widely studied crop for its own genome and has tremendous diversity and variant, maize is considered as a forefront crop in development and estimation of molecular markers for agricultural useful trait in genetics and breeding. Using QTL (Quantitative Trait Loci) and MAS (Marker Assisted Breeding), molecular breeders are able to accelerate the development of drought tolerance or downy mildew resistance maize genotype. The present paper overviews QTL/MAS approaches towards improvement of maize production against drought and downy mildew. We also discuss here the trends and importance of molecular marker and mapping population in maize breeding.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.189-197
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• 2018

Objective: Hemicastration is a unilateral orchiectomy to remove an injured testis, which can induce hormonal changes and compensatory hypertrophy of the remaining testis, and may influence spermatogenesis. However, the underlying molecular mechanisms are poorly understood. Here, we investigated the impact of hemicastration on remaining testicular function. Methods: Prepubertal mice (age 24 days) were hemicastrated, and their growth was monitored until they reached physical maturity (age 72 days). Subsequently, we determined testis DNA methylation patterns using reduced representation bisulfite sequencing of normal and hemicastrated mice. Moreover, we profiled the testicular gene expression patterns by RNA sequencing (RNA-seq) to examine whether methylation changes affected gene expression in hemicastrated mice. Results: Hemicastration did not significantly affect growth or testosterone (p>0.05) compared with control. The genome-wide DNA methylation pattern of remaining testis suggested that substantial genes harbored differentially methylated regions (1,139) in gene bodies, which were enriched in process of protein binding and cell adhesion. Moreover, RNA-seq results indicated that 46 differentially expressed genes (DEGs) involved in meiotic cell cycle, synaptonemal complex assembly and spermatogenesis were upregulated in the hemicastration group, while 197 DEGs were downregulated, which were related to arachidonic acid metabolism. Integrative analysis revealed that proteasome 26S subunit ATPase 3 interacting protein gene, which encodes a protein crucial for homologous recombination in spermatocytes, exhibited promoter hypomethylation and higher expression level in hemicastrated mice. Conclusion: Global profiling of DNA methylation and gene expression demonstrated that hemicastration-induced compensatory response maintained normal growth and testicular morphological structure in mice.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.73-76
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• 2006

This study was carried out to investigate the change yield and grain quality in rice caused by damages of brown planthopper (Bph) on major agronomic traits at the ripening stage. A major agronomic traits such as culm length, panicle length, number of panicle and number of grain per panicles was no-significant difference compared hopperburn area (HBA) and moderately damaged area (MDA) by brown planthopper with non-damaged area (NDA). but, the ripeing ratio and yield in HBA and MDA was significantly decreased. The perfect rice of brown rice in HBA and MDA was also lowered than the NDA and the immatured grain among imperfect rice in damaged area by BPH was significantly increased than in the NDA. Among the traits related with palatability of cooked rice, the amylose content was not affected by damage of brown planthopper, whereas, the protein content was significantly increased in the HBA and MDA. additionally, Peak viscosity in HBA and MDA is lower than the NDA, and setback was increased. In most cases, there was negative on yield and grain quality by the damage of brown planthopper at the ripening stage. Thus, the rice product harvested in damaged area should be separated with that in non damaged area for grain market quality of milled rice.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1529-1535
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• 2013

The TBC1D1 plays a key role in body energy homeostasis by regulating the insulin-stimulated glucose uptake in skeletal muscle. The present study aimed to identify the association between genetic polymorphisms of TBC1D1 and body weight (BW) in rabbits. Among the total of 12 SNPs detected in all 20 exons, only one SNP was non-synonymous (c.214G>A. p.G72R) located in exon 1. c.214G>A was subsequently genotyped among 491 individuals from two rabbit breeds by the high-resolution melting method. Allele A was the predominant allele with frequencies of 0.7780 and 0.6678 in European white rabbit (EWR, n = 205) and New Zealand White rabbit (NZW, n = 286), respectively. The moderate polymorphism information content (0.250.05). Our results implied that the c.214G>A of TBC1D1 gene might be one of the candidate loci affecting the trait of 35 d BW in the rabbit.

• The Plant Pathology Journal
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• v.37 no.2
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• pp.200-203
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• 2021

Rice blast caused by the filamentous fungus Magnaporthe oryzae, is arguably the most devastating rice disease worldwide. Development of a high-throughput and reliable field blast resistance evaluation system is essential for resistant germplasm screening, resistance genes identification and resistant varieties breeding. However, the occurrence of rice blast in paddy field is easily affected by various factors, particularly lack of sufficient inoculum, which always leads to the non-uniform occurrence and reduced disease severity. Here, we described a procedure for adequately inducing the occurrence of rice seedling blast in paddy field, which involves pretreatment of diseased straw, initiation of seedling blast for the first batch of spreader population, inducing the occurrence of the second batch of spreader population and test materials. This procedure enables uniform and consistent infection, which facilitates efficient and accurate assessment of seedling blast resistance for diverse rice materials.