• Asian-Australasian Journal of Animal Sciences
• /
• 제28권3호
• /
• pp.328-333
• /
• 2015

A genome wide association study was conducted using estimated breeding value (EBV) for milk production traits from 1st to 4th lactation. Significant single nucleotide polymorphism (SNP) markers were selected for each trait and the differences were compared by lactation. DNA samples were taken from 456 animals with EBV which are Holstein proven bulls whose semen is being sold or the daughters of old proven bulls whose semen is no longer being sold in Korea. High density genome wide SNP genotype was investigated and the significance of markers associated with traits was tested using the breeding value estimated by a multiple lactation model as a dependent variant. As the result of significance comparisons by lactations, several differences were found between the first lactation and subsequent lactations (from second to 4th lactation). A similar trend was noted in mean deviation and correlation of the estimated effects by lactation. Since there was a difference in the genes associated with EBV for each trait between first and subsequent lactations, a multi-lactation model in which lactation is considered as a different trait is genetically useful. Also, significant markers in all lactations and common markers for different traits were detected, which can be used as markers for quantitative trait loci exploration and marker assisted selection in milk production traits.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권10호
• /
• pp.1483-1490
• /
• 2019

Objective: To explore the molecular mechanisms of fat metabolism and deposition in pigs, an experiment was conducted to identify hepatic mRNAs and miRNAs expression and determine the potential interaction of them in two phenotypically extreme pig breeds. Methods: mRNA and miRNA profiling of liver from 70-day Jinhua (JH) and Landrace (LD) pigs were performed using RNA sequencing. Blood samples were taken to detect results of serum biochemistry. Bioinformatics analysis were applied to construct differentially expressed miRNA-mRNA network. Results: Serum total triiodothyronine and total thyroxine were significantly lower in Jinhua pigs, but the content of serum total cholesterol (TCH) and low-density lipoprotein cholesterol were strikingly higher. A total of 467 differentially expressed genes (DEGs) and 35 differentially expressed miRNAs (DE miRNAs) were identified between JH and LD groups. Gene ontology analysis suggested that DEGs were involved in oxidation-reduction, lipid biosynthetic and lipid metabolism process. Interaction network of DEGs and DE miRNAs were constructed, according to target prediction results. Conclusion: We generated transcriptome and miRNAome profiles of liver from JH and LD pig breeds which represent distinguishing phenotypes of growth and metabolism. The potential miRNA-mRNA interaction networks may provide a comprehensive understanding in the mechanism of lipid metabolism. These results serve as a basis for further investigation on biological functions of miRNAs in the porcine liver.

• 한국환경생태학회지
• /
• 제22권6호
• /
• pp.648-657
• /
• 2008

본 연구는 제주도와 인근 부속도서에 서식하는 까치의 번식 밀도와 계절별 식이물을 조사하기 위하여 이루어졌다. 번식 밀도를 알아보기 위한 둥지 분포 조사는 까치의 번식기인 2008년 2월부터 4월까지, 식이물 조사는 2006년 5월부터 2008년 2월까지 이루어졌다. 조사결과, 제주도 전역에서 관찰된 둥지 수는 모두 2,113개로 둥지의 평균 밀도는 1.33개/$km^2$였으며, 해발 600여 미터까지 분포하는 것으로 나타났다. 둥지 밀도는 제주시 중부지역이 688개소, 3.61개/$km^2$로 가장 높은 반면, 제주시 동부지역이 214개소, 0.66개/$km^2$로 가장 낮은 밀도를 보였다. 해발 고도별 둥지의 밀도는 100m 미만에서 1,172개소, 1.85개/$km^2$로 가장 높았고, 해발 500-600m에서 16개소, 0.20개/$km^2$로 가장 낮게 나타났다. 제주도내 유인도에서 관찰된 둥지 밀도는 비양도 8개소로 15.38개/$km^2$, 우도 9개소로 1.49개/$km^2$, 가파도 1개소로 1.15개/$km^2$였고, 마라도에서는 관찰되지 않았다. 위 내용물은 개체수 파악이 불가능한 뼈, 조류알껍데기, 식물, 종자를 포함하여 모두 17종류였으며, 그 중 딱정벌레류가 가장 많았다. 봄과 여름에는 무척추동물인 곤충류의 포식빈도는 높았지만, 겨울에는 30% 내외로 낮았다. 식물과 종자의 빈도는 봄에 각각 10%와 30%로 낮게 나타났으며, 겨울에는 모두 100%로 가장 높은 빈도를 보였다. 그리고 포식된 택이원 중 빈도는 크게 나타나지 않았지만 조류의 알과 뼈도 관찰되었다. 생태계 내에서 상위 포식자 역할을 하고 있는 까치 개체군 밀도의 증가는 소형 조류나 소형 파충류 등의 토착종의 종수와 개체수의 감소에 직접적으로 영향을 미칠 것이 예상되어 이에 대한 장기 모니터링 및 대책 마련이 필요하다고 판단된다.

• 한국작물학회지
• /
• 제53권3호
• /
• pp.326-332
• /
• 2008

영남평야지 조기재배시 국내 육성된 고품질 조생종 품종들의 품질에 미치는 영향을 살펴본 결과를 요약하면 아래와 같다. 1. 공시품종들의 평균 출수기는 7월 22일로 8월 하순에서 9월 상순사이에 수확이 가능한 것으로 판단되었고, 공시품종들의 평균 수당립수, 등숙율 및 수량에서 품종간 유의한 차이가 있었으며, 운광벼가 수량이 529 kg/10a로 가장 많았다. 2. 수확시기별 현미 품위를 조사한 결과 동할미 발생을 줄이고 현미 정립율을 높이기 위해서는 출수 후 40일경에 수확하는 것이 정립은 8월25일$\sim$ 9월1일 사이 수확하는 것이 가장 높았으며, 수확시기가 늦어질수록 미숙립의 발생은 감소하고 동할립의 발생은 증가하였다. 백미 완전미는 중화벼와 고운벼가 72.2%와 71.0%로 높았으나 싸라기 발생이 높았으며, 운광벼와 오대벼는 분상질의 발생이 많았다. 3. 운광벼와 오대벼는 백미 단백질 함량은 6.4%로 낮았으며, 식미관능검정의 총평이 각0.6 및 0.5로 밥맛이 양호하였다.

• Journal of Animal Science and Technology
• /
• 제62권6호
• /
• pp.777-789
• /
• 2020

Intense artificial selection has been imposed to Luzhong mutton sheep population in the past years. Improvements on growth and reproductive performance are two breeding goals in the present herd. Although some progresses were phenotypically observed possibly due to inbreeding induced by strong selection in terms of these traits, the genomic evaluation was poorly understood. Therefore, a high-density SNP array was used to characterize the pattern of runs of homozygosity (ROH), estimate inbreeding and inbreeding depressions on early growth performance and litter size based upon ROH, and scan positive selection signatures of recent population. Consequently, a low inbreeding level was observed which had negative effects on litter size, but not on early growth performance. And 160 genes were under selection, of which some were reported to be linked to several traits of sheep including body weight, litter size, carcass and meat quality, milk yield and composition, fiber quality and health, and the top genes were associated with growth (growth hormone [GH]- growth hormone receptor [GHR]- Insulin-like growth factor 1 [IGF1] axis) and litter size (bone morphogenic proteins [BMPs]-associated). The effectiveness of previous breeding measures was highlighted, but purging selection was proposed to alleviate the inbreeding depression on litter size, providing some genomic insights to breeding management of Luzhong mutton sheep.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제8권1호
• /
• pp.33-41
• /
• 2004

The type of trichomes, their density and pattern of distribution on leaves of 16 genotypes of mulberry, belonging to both diploid and polyploid categories, were studied by scanning electron microscope. The present investigation was undertaken to find out the relationship of physical attributes, especially the density and trichome types with higher acceptability and better rearing performance by the silkworm Bombyx-mori L. Two types of trichomes glandular and non-glandular types were observed on both the leaf surfaces of all the mulberry genotypes studied. In general, greater densities of trichomes were observed on the abaxial surface than the adaxial surface of leaves in most of the genotypes. Distribution of glandular trichomes were more in abaxial surface and non-glandular trichomes were more in adaxial surface. Overall, distribution of glandular and non-glandular trichomes per unit area of leaf did not follow any regular pattern. When leaves of those genotypes were fed to silkworms, trichome density was found to be significantly negatively correlated with the survival of larvae i.e., effective rate of rearing, but trichome density did not influence the economic characters of rearing. As the distribution of glandular trichomes (GT) and non-glandular trichomes (NGT) did not follow any definite pattern, no relation could be established between the GT and NGT densities with silkworm rearing performance. However, the ratio of GT and NGT in a particular genotype influenced the rearing parameters, higher the ratios better the rearing performance. High GT and NGT ratio (＞1.00) was found positively significant when correlated with economic parameters viz., larval weight, single cocoon weight and single shell weight. The study is useful in screening different mulberry genotypes for their better acceptability to silk-worm and higher rearing performance at the early stage of selection without actually conducting the rearing.

• 한국습지학회지
• /
• 제11권1호
• /
• pp.29-37
• /
• 2009

본 연구는 경기도의 경안천 습지에서 번식 조류의 둥지 장소로서의 정수식물에 대한 선호성을 알아보고자 수행되었다. 6종의 조류가 정수식물에 번식하였으며 물닭 Fulica atra, 쇠물닭 Gallinula chloropus, 개개비 Acrocephalus orientalis, 덤불해오라기 Ixobrychus eurhythmus, 논병아리 Podiceps ruficollis, 뿔논병아리 Podiceps cristatus 등의 둥지가 발견되었다. 둥지 장소로 이용한 곳은 애기부들 Typha angustata, 갈대 Phragmites communis 와 줄플 Zizania latitolia 이었다. 특히 애기부들은 번식조류가 가장 선호하는 곳이었다. 그에 비해 쓰레기가 쌓인 곳이나 밀도가 빽빽한 곳은 둥지가 거의 없었다. 내부의 덤불에서는 둥지가 전혀 발견 되지 않았다. 소택지 내 둥지의 평균 밀도는 3.1개였으며 논섬이 17개/ha 로 밀도가 가장 높았다. 둥지 밀도는 소내섬 주변 지역이 광동교 인근보다 높았다. 이것은 애기부들의 규모와 수질과 관련될 것으로 보인다.

• 한국수산과학회지
• /
• 제56권4호
• /
• pp.541-548
• /
• 2023

Behavior serves as an important indicator of the effects of environmental stressors on organisms. In this study, one-year-old abalone Haliotis discus hannai (15.1±0.8 mm) were used and each 0.34 m2 tank was stocked with 100, 150, 200, 250, and 300 juvenile abalone. After 328 days, we found that the low stocking density treatment showed significantly higher growth, whereas the high stocking density treatment showed significantly lower growth. In addition, abalone showed a lower rate of movement, distance moved, and velocity at higher stocking densities, but only velocity differed significantly. In contrast, In the Supplied the feed tanks significant difference between the moving distance and velocity. When the two groups of different sizes were mixed in tank, the rate of movement, distance moned, and velocity showed significant differences between differently-sized indivisuals. Our study indicates that breeding abalone at high densities has a negative effect on their growth and survival, which is thought to primarily be caused by spatial competition, and secondarily by differences in body size, ultimately affecting growth and productivity.

• Nuclear Engineering and Technology
• /
• 제55권8호
• /
• pp.2966-2976
• /
• 2023

A tritium breeder is a lithium-based material capable of producing tritium. Many researchers designing nuclear-fusion energy are studying tritium production using pebbles, which are solid-type breeders. The sphericity and size of the pebbles are critical in obtaining pebbles with good tritium-breeding efficiency. Furthermore, tritium-release efficiency can be increased by using pebbles with appropriate porosities. Promising raw materials for tritium-breeding materials include Li₄SiO₄ and Li₂TiO₃. Li₄SiO₄ has a higher lithium density than Li₂TiO₃ and exhibits excellent tritium-breeding efficiency. However, it has the disadvantage of being easily decomposed during the Li₄SiO₄-green-pebble sintering process because of its low structural stability at high temperatures and high lithium density. In this study, we attempted to determine the optimal conditions for manufacturing Li₄SiO₄ pebbles using the droplet-freeze-drying method. The optimal Li₄SiO₄ slurry conditions and sintering temperatures were determined. The optimal Li₄SiO₄ slurry-fabrication conditions were 3 wt% polyvinyl alcohol and 75 wt% Li₄SiO₄ based on the deionized-water weight content. The sintering temperature at which Li₄SiO₄ did not decompose and exhibited the optimum porosity of 10.8% was 900 ℃.

• Genomics & Informatics
• /
• 제19권3호
• /
• pp.33.1-33.10
• /
• 2021

Eucalyptus is one of the major plantation species with wide variety of industrial uses. Polymorphic and informative simple sequence repeats (SSRs) have broad range of applications in genetic analysis. In this study, two individuals of Eucalyptus tereticornis (ET217 and ET86), one individual each from E. camaldulensis (EC17) and E. grandis (EG9) were subjected to whole genome resequencing. Low coverage (10×) genome sequencing was used to find polymorphic SSRs between the individuals. Average number of SSR loci identified was 95,513 and the density of SSRs per Mb was from 157.39 in EG9 to 155.08 in EC17. Among all the SSRs detected, the most abundant repeat motifs were di-nucleotide (59.6%-62.5%), followed by tri- (23.7%-27.2%), tetra- (5.2%-5.6%), penta- (5.0%-5.3%), and hexa-nucleotide (2.7%-2.9%). The predominant SSR motif units were AG/CT and AAG/TTC. Computational genome analysis predicted the SSR length variations between the individuals and identified the gene functions of SSR containing sequences. Selected subset of polymorphic markers was validated in a full-sib family of eucalypts. Additionally, genome-wide characterization of single nucleotide polymorphisms, InDels and transcriptional regulators were carried out. These variations will find their utility in genome-wide association studies as well as understanding of molecular mechanisms involved in key economic traits. The genomic resources generated in this study would provide an impetus to integrate genomics in marker-trait associations and breeding of tropical eucalypts.