• Bulletin of the Society of Naval Architects of Korea
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• v.6 no.1
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• pp.25-34
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• 1969

From viewpoints of over-all ship economy the straight framed V-bottom hull forms with chines are considered to be attractive even for usual commercial vessels, because increments of resistance over that of round hull forms, if any, can be well compensated with reduction in construction cost.[1] To investigate the influences of both prismatic coefficient and chine elevation on resistance performance, three models of straight-framed V-bottom hull forms which are similar to Prof. C. Ridgely-Nevitt's W-18, W-8, and W-20[2],[3] in size and hull form coefficients were tested at the SNU Ship Model Towing Tank for resistance measurements. They are of Cp=0.60, 0.65 and 0.70 and of ${\Delta}/(0.01L)^3=300$. Influence of variation of chine elevation on resistance performance were observed with the test results obtained at normal condition, and at the trimed by the stern by 2% and 4% of $L_{bp}$ at normal condition under same displacement. The hull form characteristics are shown in Table 1, and in Fig. 1, 2, 3, 4 and 5. The test results are shown in Fig 8, 9 and 10 in the form of Cr vs. $V/\sqrt{L}$ curves taking Cp as a parameter for normal condition, trim by the stern in 2% and 4% $L_{bp}$ at normal condition , respectively. Cr vs. $V/\sqrt{L}$ curves taking trim condition as a parameter are also shown in Fig 11, 12 and 13 for Cp=0.60 and 0.70, respectively. The best and the worst trim condition at given $V/\sqrt{L}$ in viewpoint of Cr are plotted for each Cp-value as shown in Fig 14, 15 and 16. From the above results the following conclusions are derived: (1) In general, the resistance performance of the straight-framed V-bottom hull forms are not inferior to those of round hull forms. At a certain range of $V/\sqrt{L}$ the former gives less resistance than the latter. (2) Regarding influences of Cp on Cr, it is observed that, at $V/\sqrt{L}$ less than about 0.925, the greater Cp-value gives the more increment of Cr, and that, at $V/\sqrt{L}$ greater than about 0.925 the smaller Cp-value gives the more increment of Cr. It is also noteworthy that the model of Cp=0.70 has remarkable hump on Cr vs. $V/\sqrt{L}$ curve between $V/\sqrt{L}=0.80$ and 0.90. (3) For higher speed within the test range, the chine elevation having the steeper slope around bow and the easier slope around amidship and stern, refered to watering, give the better results in resistance performance. (4) Assuming the chine elevations adopted for the tested models were not of the best, we would expect further improvement of resistance performance for such form. Hence, a systematic study on chine elevation is very disirable to prepare design data of general purpose for the such hull forms.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.6D
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• pp.697-705
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• 2010

The purpose of this research is to clarify the relation between environmental consciousness and railway usage behavior. Author would locate this research on position of basic survey to promote railway use according to Low Carbon Green Growth policy in Korea. In this research, we would perform descriptive analysis using data of research on the actual condition of railway use in 2008, Daegu, and describe its relationship. In addition, we would suggest some idea about policy which can promote railway use. The order of railway choice behavior noticed in clustering of environmental consciousness was cooperative behavior type, middle type and non-cooperative behavior type. It suggests that environmental consciousness has effect on transportation choice behavior. Specially, railway improvement isn't enough to promote railway use. And, it is advisable to carry out the improvement in such a way that it may encourage the nation to move from the current environmental consciousness stage to cooperative behavior. Moreover, we assumed Binary Probit(BP) model using SP data of time or condition of transportation expense compared with passenger car and bus. As the results, modified likelihood ratio of two BP models is favorable variables. And it occurred that mode was transferred from passenger car to railway when it showed higher social environment consciousness and low selfish environment consciousness, because t-statistic which represents selfish environment consciousness showed significance in 95% confidence level. That is, it can be described that environment consciousness affect on the intention of railway use.

• Microbiology and Biotechnology Letters
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• v.38 no.2
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• pp.168-176
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• 2010

Acellular dermal matrix (ADM), produced by decellularization from human cadaveric skin, has been used for various biomedical applications. A manufacturing process for ADM ($SureDerm^{TM}$) using tri-n-butyl phospahate (TnBP) and deoxycholic acids as the decellularization solution has been developed. The manufacturing process for $SureDerm^{TM}$ has 70% ethanol treatment and ethylene oxide gas sterilization for inactivating infectious microorganisms. The purpose of this study was to examine the efficacy of the 70% ethanol treatment, decellularization process using 0.1% TnBP and 2% deoxycholic acids, and EO gas sterilization process in the inactivation of viruses. A variety of experimental model viruses for human pathogens, including the human immunodeficiency virus type 1 (HIV-1), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), and porcine parvovirus (PPV) were all selected for this study. Enveloped viruses such as HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by 70% ethanol treatment. However HAV and PPV showed high resistance to 70% ethanol treatment with the log reduction factors of 1.85 and 1.15, respectively. HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by decellularization process. All the viruses tested were completely inactivated to undetectable levels by EO gas treatment. The cumulative log reduction factors of HIV-1, BHV, BVDV, HAV, and PPV were $\geq12.71$, $\geq18.08$, $\geq14.92$, $\geq6.57$, and $\geq7.18$, respectively. These results indicate that the production process for $SureDerm^{TM}$ has a sufficient virus-reducing capacity to achieve a high margin of the virus safety.

• Applied Chemistry for Engineering
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• v.18 no.2
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• pp.168-172
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• 2007

The separation of indole from a model mixture comprising four kinds of nitrogen heterocyclic compounds [indole (In), quinoline (Q), iso-quinoline (iQ), quinaldine (Qu)], three kinds of bicyclic aromatic compounds [1-methylnaphthalene (1MN), 2-methylnaphthalene (2MN), dimethylnaphthalene (DMN)], biphenyl (Bp) and phenyl ether (Pe) was examined by batch cocurrent 4 stages equilibrium extraction. The model mixture used as a raw material in this work was prepared according to the components and compositions contained in coal tar fraction (the temperature ranges of fraction: $240{\sim}265^{\circ}C$). An aqueous solution of formamide was used as a solvent. Indole was recovered more than 99% through 4 stages of the equilibrium extraction. The range of selectivity of indole in reference to DMN obtained through the 5 stages equilibrium extraction was found to be 63~118. The process for separation and recovery of indole contained in coal tar was studied by using the experimental results obtained from this work and the previous work.

• Journal of the Korean Data and Information Science Society
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• v.28 no.6
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• pp.1327-1336
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• 2017

In this study, the problems in the short term stock market forecasting are analyzed and the feasibility of the ARIMA method and the backpropagation neural network is discussed. Neural network and genetic algorithm in short term stock forecasting is also examined. Since the backpropagation algorithm often falls into the local minima trap, we optimized the backpropagation neural network and established a genetic algorithm based on backpropagation neural network for forecasting model in order to achieve high forecasting accuracy. The experiments adopted the korea composite stock price index series to make prediction and provided corresponding error analysis. The results show that the genetic algorithm based on backpropagation neural network model proposed in this study has a significant improvement in stock price index series forecasting accuracy.

• The KIPS Transactions:PartC
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• v.10C no.4
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• pp.423-432
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• 2003

It is essential suity management and standardized asset analysis for telecommunication networks, however existing risk analysis methods and tools are not enough to give shape of the method to evaluate value and asset. they only support asset classification schemes. Moreover, since the existing asset classification schemes are to evaluate comprehensive general risk, they are not appropriate for being applied telecommunication networks and they can´t offer any solutions to an evaluator´s subjectivity problem. In this paper, to solve these problems, we introduce the standardized definition of asset evaluation model new asset classification scheme, two-dimensional asset process classification scheme to consider business process and asset, various evaluation standards for quantitative value and qualitative evaluation. To settle an valuator´s subjectivity problem, we proposed $\beta$-distribution Delphi method.

• Journal of Plant Biotechnology
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• v.42 no.2
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• pp.93-103
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• 2015

The isolation, cloning, and characterization of an R2R3-MYB transcription factor gene (MtMYB70) from the model legume Medicago truncatula is reported. MtMYB70 consists of a 768-bp coding sequence corresponding to 255 amino acids. Sequence alignment revealed that MtMYB70 cDNA contains conserved R2R3-type MYB domains with highly divergent C terminal regions. MtMYB70 was found to have relatively low sequence homology with known R2R3-MYB genes. Phylogenetic analysis placed the R2R3-MYB domain of MtMYB70 closest to PtMYB1, a known activator of lignin biosynthesis. Overexpression of MtMYB70 under the control of the 35S promoter in transgenic poplar did not cause a significant difference in total lignin content relative to the control, but glucan content was significantly increased in transgenic poplar. Therefore, MtMYB70 might have regulatory role in the biosynthesis of cell wall structural carbohydrates.

• BMB Reports
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• v.39 no.5
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• pp.578-585
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• 2006

The cDNA of a firefly luciferase from lantern mRNA of Lampyroidea maculata has been cloned, sequenced and functionally expressed. The cDNA has an open reading frame of 1647 bp and codes for a 548-residue-long polypeptide. Noteworthy, sequence comparison as well as homology modeling showed the highest degree of similarity with H. unmunsana and L. mingrelica luciferases, suggesting a close phylogenetic relationship despite the geographical distance separation. The deduced amino acid sequence of the luciferase gene of firefly L. maculata showed 93% identity to H. unmunsana. Superposition of the three-dimensional model of L. maculata luciferase (generated by homology modeling) and three dimensional structure of Photinus pyralis luciferase revealed that the spatial arrangements of Luciferin and ATP-binding residues are very similar. Putative signature of AMP-binding domain among the various firefly species and Lampyroidea maculata was compared and a striking similarity was found. Different motifs and sites have been identified in Lampyroidea maculata by sequence analysis. Expression and purification of luciferase from Lampyroidea maculata was carried out using Ni-NTA Sepharose. Bioluminescence emission spectrum was similar to Photinus pyralis luciferase.

• Ocean Systems Engineering
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• v.4 no.1
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• pp.53-62
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• 2014

The detection of ship wakes image can demonstrate substantial information regarding on a ship, such as its tonnage, type, direction, and speed of movement. Consequently, the wake target recognition is a favorable way for ship identification. This paper proposes a Local Binary Pattern (LBP) approach to extract image features (wakes) for training an Improved Back Propagation Artificial Neural Network (IBPANN) to identify ship speed. This method is applied to sort and recognize the ship wakes of five different speeds images, the result shows that the detection accuracy is satisfied as expected, the average correctness rates of wakes target recognition at the five speeds may be achieved over 80%. Specifically, the lower ship's speed, the better accurate rate, sometimes it's accuracy could be close to 100%. In addition, one significant feature of this method is that it can receive a higher recognition rate than the nearest neighbor classification method.

• BMB Reports
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• v.34 no.6
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• pp.502-508
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• 2001

A cDNA, encoding the human growth hormone (hGH), was synthesized based on the known 191 amino acid sequence. Its codon usage was optimized for a high level expression in Escherichia coli. Unique restriction sites were incorporated throughout the gene to facilitate mutagenesis in further studies. To minimize an initiation translation problem, a 624-bp cassette that contained a ribosome binding site and a start codon were fused to the hGH-coding sequence that was flanked between the EcoRI and HindIII sites. The whole fragment was synthesized by an overlapped extension of eight long synthetic oligonucleotides. The four-short duplexes of DNA, which were first formed by annealing and filling-in with a Klenow fragment, were assembled to form a complete hGH gene. The hGH was cloned and expressed successfully using a pET17b plasmid that contained the T7 promoter. Recombinant hGH yielded as much as 20% of the total cellular proteins. However, the majority of the protein was in the form of insoluble inclusion bodies. N-terminal amino acid sequencing also showed that the hGH produced in E. coli contained formyl-methionine. This study provides a useful model for synthesis of the gene of interest and production of recombinant proteins in E. coli.