• 한국의학물리학회지:의학물리
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• 제25권4호
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• pp.225-232
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• 2014

본 논문에서는 자체 제작된 선량상승영역 변환기(build-up modifier, BM)을 투과하는 high energy photon beam의 심부선량백분율(PDD)을 특성을 측정하고 이 결과를 토대로 BM 산란인자(BM scatter factor, $S_{BM}$)를 계산하였다. 다양한 조건에서 BM scatter가 PDD의 Build-up region에 미치는 영향을 평가하고 BM의 유용성을 알아보는 것이 본 연구의 목적이다. $S_{BM}$는 BM을 사용하지 않은 SFS 30 mm에서 측정된 산란인자의 값을 1로서 정규화 하였다. 가장 큰 SFS 200 mm의 경우, 6 MV 광자선을 사용할 때 $S_{BM}$는 두께에 따라 각각 1.331, 1.519, 1.598, 1.641, 그리고 1.657이었다. 10 MV 광자선에는 각각 1.384, 1.662, 1.825, 1.913, 그리고 2.001이었다. BM의 효과는 bolus의 최대 76% 효율을 가지는 것으로 나타났다. Bolus를 밀착시키기 어려운 특정적 부위에 대해 BM은 그 대안으로써 효과적인 장치가 될 수 있을 것으로 기대된다.

• 한국전자거래학회지
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• 제17권4호
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• pp.175-196
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• 2012

정부와 산업에서 융합 내지 융합 비즈니스에 대한 정책과 전략이 광범위하게 확산되고 있음에 반해 이를 성공적으로 구현하는데 필요한 이론적 기반과 효과적 방법론은 매우 부족한 상태이다. 특히, 융합 비즈니스 모델(BM)의 '이상적(ideal)유형' 즉, 이론적 유형에 대한 연구는 국내/외를 막론하고 아직까지 주목할 만한 결과가 없는 것으로 파악된다. 본 논문은 BM의 개념적 프레임워크로 '4W1H 모형'을 제안하고 이를 통해 융합 BM의 이론적 유형, BM 유형별 전략과 설계 수순 등을 정의할 수 있음을 보이기 위한 것이다. 4W1H 모형은 BM의 구성요소를 고객가치(why), 가치제안(what), 운영방식(how), 목표시장(whom), 공급역량(who) 등 5개로 정의한 것으로서 이를 통해 가치혁신형, 상품혁신형, 운영혁신형, 시장혁신형, 역량혁신형 등의 융합 BM 유형을 도출하였다. BM 구성요소와 유형(즉, 구조적 특성) 정의에 이어 BM 구성 요소간 상호작용(즉, 행위적 특성)에 대한 정의를 통해 BM이 기업전략과도 연계될 수 있음을 보였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.131-135
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• 2007

Open reading frame 4 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm4, is a gene whose function is completely unknown. With the recently developed BmNPV bacmid and a modified pFastBac1 whose polyhedrin promoter was replaced with ie1 promoter, a recombinant bacmid expressing Bm4-EGFP fusion protein under the control of ie1 promoter in BmN cells was successfully constructed. The result not only showed that the polyhedrin promoter can be replaced efficiently with other promoters to direct the expression of foreign gene in BmN cells by using Bac-to-Bac/BmNPV baculovirus expression system but also laid the foundation for rescue experiment of Bm4 deletion mutant due to the ability of ie1 promoter to direct gene expression throughout the infection cycle.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권1호
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• pp.35-40
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• 2004

Bombyx mori densonucleosis virus type 1 (BmDNV1)- a non occluded virus causes flacherie disease in the susceptible stocks of the silkworm, Bombyx mori. However, some stocks are non-susceptible. Non-susceptibility to BmDNV1 in B. mori is a unique case where the virus infection is completely inhibited by a single gene of the host. A survey conducted by this institute in some parts of Karnataka state has revealed that, 43.05% of the total incidence of flacherie disease caused by non-occluded viruses, are due to the synergistic infection of B. mori densonucleosis and infectious flacherie virus. Earlier study indicated that rearing of BmDNV1 resistant silkworm stock is effective in protecting silkworm against BmIFV also. In the present study the response of 78 silkworm stocks which include 42 of non-diapausing and 36 of diapausing groups, to BmDNV1 is investigated. Newly ecdysed third instar larvae were inoculated per-os with 10% inoculum of BmDNV1 extracted from the mid-gut of infected silkworm. One non-diapausing and three diapausing silkworm stocks were found to be resistant to BmDNV1. Eleven silkworm stocks were found to possess moderate resistance whereas rest sixty three were found to be susceptible to BmDNV1. Genetic analysis has shown that the resistance to BmDNV1 is autosomally inherited and controlled by a major dominant or a major recessive gene in different silkworm stocks. These resistant stocks can be utilized as the resource material to develop BmDNV1 resistant commercial hybrids. The selection strategies, depending upon the mode of inheritance of resistance in the resource material chosen, are discussed.

• 한국잠사곤충학회지
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• 제33권1호
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• pp.21-26
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• 1991

Bm 배양세포주(BmN4)에서 BmNPV의 다각체 단백질 합성과 DNA복제에 대한 실험결과는 다음과 같다. 1. BmNPV는 insect Grace's medium에서 배양되고 있는 BmN4 세포에서 NOV나 DNA로 감염시킨 경우 모두 잘 증식되었으며, 도립현미경 관찰 결과 접종 후 48시간이 경과하면서 다각체가 형성되기 시작하였다. 2. 또한 전자현미경으로 핵내 형성된 다각체와 협성중인 nucleocapsid 다발을 관찰하였으며, 바이러스 입자는 SNPV로 존재하였다. 3. Western blot분속에 의한 다각체 단백질의 합성은 접종 후 18시간부터 관찰되었으며, 다각체 단백질의 분자량은 30kd이었다. 4. Wild type BmNPV DNA와 Bm 세포(BmN4)에서 NOV 및 DNA 감염에 의해 형성된 바이러스 DNA간의 제한효소 패턴은 차이가 없었다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1535-1539
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• 2003

Four microsatellite DNA loci BM1818, BM1258, BM1443 and BM1905 associated with the somatic cell counts (SCC) in cow milk were analyzed for genetic variation in 240 Beijing Holstein cows. The PCR amplified products of microsatellites DNA were detected by non-denatured polyacrylamide gel electrophoresis. The number of alleles for BM1818, BM1258, BM1443 and BM1905 were 4, 5, 8 and 6 in Beijing Holstein cows, respectively. The allele size ranges for BM1818, BM1258, BM1443 and BM1905 were 274 bp to 286 bp, 92 bp to 106 bp, 154 bp to 170 bp and 187 bp to 201 bp, respectively. The polymorphism information content/effective number of alleles/heterozygosity for BM1818, BM1258, BM1443 and BM1905 were 0.3869/1.7693/0.4348, 0.5923/2.9121/0.6566, 0.7114/3.9012/0.7437 and 0.5921/2.8244/0.6459. These data showed the microsatellite DNA locus BM1443 has the highest variability, followed by BM1258, BM1905 and BM1818. The results of the least squares means analysis showed as follows: the least squares mean of SCC for BM1818 284 bp/284 bp was significantly lower than that for BM1818 286 bp/286 bp (p<0.05). The least squares mean of SCC for BM1258 100 bp/100 bp was significantly lower than that for BM1258 102 bp/102 bp, 106 bp/106 bp, 106 bp/104 bp, 106 bp/102 bp, 106 bp/100 bp, 104 bp/100 bp (p<0.05). The least squares mean of SCC for BM1443 166 bp/160 bp and 166 bp/166 bp was significantly lower than that for BM1443 170 bp/160 bp, 160 bp/157 bp, 165 bp/160 bp (p<0.05). The least squares mean of SCC for BM1905 187 bp/187 bp was significantly lower than that for BM1905 197 bp/195 bp, 193 bp/187 bp (p<0.05).

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.25-29
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• 2001

We have cloned and characterized an antiapoptotic gene, p35, which blocks apoptosis, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 897 bp p35 has an open reading frame of 299 amino acids. The BmNPV-K1 p35 showed a high identity to Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain. The BmNPV-K1 p35 was different from the amino acid sequences of BmNPV T3 at 6 positions. The p35 gene of BmNPV-K1 was 99.2% identical at the nucleotide level and 98% identical at the amino acid level to BmNPV T3. The location of p35 gene in the BmNPV-K1 genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level in the infected cells were con- firmed by Northern hybridization analysis.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2003년도 춘계종합학술대회
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• pp.406-409
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• 2003

TFF(Thin Film Fille.) 방식의 2$\times$2 OADM을 이용하여 1510nm 파장과 1530nm 파장의 광원을 첨가하여 add/drop시킨 4 파장 광 모듈을 구성하였고 이 광모듈을 채택하여 PCS(personal communication system) 및 셀룰러 (cellular) 이동 통신뿐만 아니라 차세대 이동 통신 방식은 IMT-2000 서비스와 다중 링크 시켜 광선로를 재활용하는 방식의 4 파장 광 트랜시버를 구현하였다. OADM을 이용한 4파장 optical signal stream을 구성하여 1510 nm, 1530 nm, 1550 nm, 1570 nm 파장에서 각각 -1.6 dBm, -1.7 dBm, -5.6 dBm, -5.8dBm의 광출력을 얻었다. 제작된 광 트랜시버의 LD 모듈에 12 V의 바이어스 전압을 사용하여 약 1.5dBm의 광 출력 특성을 얻었으며, PD 모듈의 증폭단에는 two-stage 저 잡음 증폭기를 제작하여 1.66dB의 잡음지수와 25.7 dB의 이득을 얻었다. 또한 S$_{11}$과 S$_{22}$ 그리고 입ㆍ출력 정재파비는 각각 -5.47dB, -20 dB 그리고 3.27 : 1, 1.22 : 1의 결과 값을 얻었다.다.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.585-591
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• 2001

To compare the p10 promoter activity of Bombyx mori nucleopolyhedrovirus (BmNPV)K1 and K4, recombinant viruses Bm101-LacZ and Bm104-LacZ with a lacZ gene under the control of each p10 promoter were constructed. The $\beta$-galactosidase activity due to Bm101-LacZ was about 5.5- and 1.1-fold higher than that due to Bm104-LacZ and BmK1-LacZ, respectively. expressing ${\beta}$-galactosidase under the control of a polyhedrin promoter. The recombinant virus BmK1-104LacZ with the same genome structure as Bm101-LacZ, except for a p10 promoter region, produced a similar ${\beta}$-galactosidase activity to that due to Bm104-LacZ and 5.5-fold lower than that due to Bm101-LacZ. The virus yield, expression level of polyhedrin, and polyhedra productivity for each recombinant virus was almost similar. These results suggested that the difference in the expression level of ${\beta}$-galactosidase resulted from a difference in the p10 promoter regions, and that an expression vector using the p10 promoter of BmNPV-K1 could be usefully exploited in the mass production of foreign proteins with silkworm larvae by means of oral ingestion.

• International Journal of Industrial Entomology and Biomaterials
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• 제9권2호
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• pp.217-223
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• 2004

A cathepsin L-like cysteine protease, v-cath, encoded by the baculovirus has been shown to playa role in host liquefaction. We have identified a v-cath gene in the silkworm virus, Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 969 bp v-cath has an open reading frame of 323 amino acids. A putative cleavage site and catalytic sites were conserved in BmNPV-K1 v-cath. The predicted three-dimensional structure of BmNPV-K1 v-cath revealed that the overall fold of BmNPV-K1 v-cath is similar to that of other proteases of the papain family. The deduced amino acid sequence of BmNPV-K1 v-cath showed 98% and 97% protein sequence identity to BmNPV T3 strain and to Autographa californica nuclear polyhedrosis virus, respectively. The BmNPV-K1 v-cath differed at 4 amino acid positions from BmNPV T3. The v-cath gene in BmNPV-K1 genome is located on the EcoRV 6 kb and XhoI 9 kb fragments. Northern hybridization analysis of BmNPV K1 v-cath gene revealed that it is expressed late in infection.