• Biomedical Science Letters
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• v.18 no.3
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• pp.227-236
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• 2012

Previous study showed that swimming improved obesity but was not through $PPAR{\alpha}$ activation in liver and skeletal muscle in high fat diet-fed female mice with functioning ovaries as an animal model of obese premenopausal women. Thus, this study was aimed at investigation of the effects of swimming on the promotion of health and its molecular mechanism in adipose tissue of high fat diet-fed female mice. Eight-week-old female C57BL/6J mice were randomly divided into two groups (a non-swim control group and a swim group, n=8/group). Mice in the swim group swam for 2 h daily for 6 weeks in water bath with temperature of $35{\pm}1^{\circ}C$. All the animals received high fat diet (45% kcal fat) for 6 weeks. Reverse transcription-polymerase chain reaction was used to elucidate the molecular mechanism. Female mice subjected to swimming had significantly decreased body weight gain and white adipose tissue mass compared with the female control mice. Histological studies illustrated that swimming decreases the hepatic lipid accumulation. As expected, swimming did not affect the expression of mRNA levels of peroxisome proliferator-activated receptor (PPAR) ${\alpha}$ and $PPAR{\alpha}$ target genes responsible for mitochondrial fatty acid ${\beta}$-oxidation, such as carnitine palmitoyltransgerase-1 and medium chain acyl-CoA dehydrogenase in the white adipose tissue. However, mice that underwent 6-weeks of swimming exercise had decreased the mRNA expression of lipogenic genes, such as sterol regulatory element-binding proteins-1C and fatty acid synthase in comparison to sedentary control mice, with decreased $PPAR{\gamma}$ target genes involved in adipocyte-specific marker genes, such as adipocyte fatty acid binding protein and leptin in the white adipose tissue. These results suggest that swimming can effectively prevent obesity induced by high fat diet-fed, in part through down-regulation of adipogenesis and lipogenesis in white adipose tissue of female obese mice. Moreover, these results suggest that swimming maybe contributing the promotion of health through regulation of adipogenesis and lipogenesis in overweight premenopausal women.

• Korean Journal of Acupuncture
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• v.34 no.3
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• pp.146-155
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• 2017

Objectives : This study was carried out to investigate the effects of Crataegi Fructus water extract(CFWE) on hair growth in an alopecia model of C57BL/6N mice and human dermal papilla cells(hDPCs). Methods : Six-week old mice were depilated and separated in 3 groups ; CON, MXD(2% Minoxidil), and CFWE. The treatments were applied twice a day for 18 days. The hair growth was determined photographically. The hair density, thickness and length were identified by Folliscope and the weights of body were measured. In dorsal skin tissue, the expression of hair growth-related protein was analyzed by Western blot. In hDPCs with/without $IFN-{\gamma}$, cell proliferation and the expression of hair growth-related genes were analyzed. Results : We observed that CFWE promoted hair growth compared to CON. CFWE improved the hair density, thickness and length compared to CON. CFWE increased the $Wnt/{\beta}$-catenin signaling in dorsal skin. In hDPCs, CFWE accelerated the cell proliferation and inhibited $IFN-{\gamma}$-induced hDPCs degeneration. CFWE increased the mRNA expression of ${\beta}$-catenin, Axin-2, BMP-4, FGF-7, FGF-10, and ALP compared to CON and $IFN-{\gamma}$ treated cells. Conclusions : These results suggest that CFWE has a hair regrowth activity via $Wnt/{\beta}$-catenin signaling and can be useful for the treatment of alopecia.

• Journal of Acupuncture Research
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• v.18 no.3
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• pp.227-238
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• 2001

Objective : There was no report on the treatment of Fascioscapulohumeral (FSH) Muscular Dystrophy by Oriental medicine. But the treatment conducted on the patient admitted to the Sangji Oriental Medicine Hospital from January 9, 2001 to February 23, 2001, a significant treatment result was yielded and would like to suggest treatment plan for the future treatments. Methods : Under the assumption that Korean Bee-Venom Therapy may be affective for treating FSH Muscular Dystrophy, the following points were administered : SI10(노유), SI11(天宗), BL23(腎兪), BL26(關元兪), ST36(足三里), LI4(合谷), Liv3(太衝), SI9(肩貞). CFC(Carthami Flos;紅花 and Cervi Pantotrichum Cornu;鹿茸) herbal extract was treated on the other acupuncture points. Sa-Am(Four needle technique) Acupuncture (tonifying SI5 and ST42, sedating GB41 and ST43) was done every day. For herbal medicine, TaeEumIn ChoWiSeungChung-Tang was given based on the constitutional diagnosis. Results : After 7 weeks of treatment, a remarkable improvement was made for facial muscular movement and muscular strength of the scapular and another regions. Conclusions : 1. Significant improvement in the muscular strength for the case of FSH Muscular Dystrophy was obtained with through Korean Bee-Venom Therapy, Four needle technique, and herbal medication. 2. For progressive muscular dystrophy, it is necessary to practice muscular strength recovery exercise in conjunction with Korean Bee-Venom Therapy. 3. Although this case yielded favorable result, further observation and study must be made to concretely prove the effectiveness of Korean Bee-Venom Therapy for treating muscular dystrophies.

• Journal of Korean Medicine for Obesity Research
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• v.11 no.1
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• pp.47-60
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• 2011

Objectives The objective of this study was to contribute to the development of pharmacupuncture for obesity treatment by reviewing the studies of pharmacupuncture experimentations and clinical trials. Methods We searched the papers with keywords of 'obesity' and 'pharmacupuncture' in the search site, RISS, Oriental medicine advanced searching integrated system(OASIS), Korean traditional knowledge portal, the society of Korean medicine for obesity research, Korean pharmacupuncture institute, the Korean academy of oriental rehabitation medicine and Korean acupuncture & moxibustion society. Results 1. We reviewed 37 articles searched. 27 articles(73.0%) were animal experimentations, 5 articles(13.5%) were cell experimentations, 4 articles(10.8%) were clinical trials and 1 article(2.7%) was study analysis. 2. The herbs, using for animal experimentations, were atratylodes japonica, coix lachrymajobi, ephedra sinica, crataegus pinnatifida, wild ginseng and etc. Acupucture points were joksamni(ST36), zhongwan(CV12), gansoo(BL18), pungnyung(ST40), umnungchon(SP9), bisu(BL20), gokji (LI11), cheun-chu(ST25) and etc. 3. For cell experimentations, preadipocytes and adipocytes performed on cell cultures with using rats, 3T3-L1 preadipocytes and porcine skin including fat tissue were treated with fel ursi, bovis calculus, ephedrae herba, spirodelae herba, wild ginseng. 4. For clinical trials, Sangsik no.1, Bigiheo, ephedra, green tea and sweet bee venom were injected at the region where a lot of fat like zhongwan(CV12), xiawan(CV10), kwanwon(CV4), cheun-chu(ST25) and thigh. Conclusion Through animal and cell experimentations and clinical trials, the treatment of obesity using local acupuncture therapy was effective. For clinical use, however, it is considered that animal and cell experimentation and clinical trial's connection using one kind of herb and studies about more clinical trials and associated side effects are needed.

• The Journal of Korean Medicine
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• v.26 no.2 s.62
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• pp.176-181
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• 2005

Objectives: This study was designed to evaluate the effect of moxibustion on UPDRS and heart rate variability of patients with Parkinson's disease. Methods: Subjects were voluntarily recruited through newspapers and internet. All subjects were confirmed as showing idiopathic Parkinson's disease by a neurologist. Moxibustion therapy was performed 5 times a week by the patient's family at home and once a week by an oriental medical doctor in hospital. Moxibustion points were GV20, CV12, ST36, BL18, and BL20. Intensity was up to pain threshold to prevent patients getting burned. The patient's symptoms were assessed before and after 8 weeks of treatment by unified Parkinson's disease rating scale (UPDRS). Heart rate variability was measured for 5 minutes before and after the treatments. Results: Total UPDRS scores were significantly improved after 8 weeks compared to pre-treatment (p<0.05). There were significant decrease of mean heart rates (p<0.05) and increase of SDNN and TP (p<0.05) after 8 weeks' moxibustion treatments. There were no significant changes in other components of heart rate variability. Conclusions: This study suggests that moxibustion treatments can be applicable to improve not only symptoms but also heart rate variability in the patients with idiopathic Parkinson's disease.

• The Journal of Pediatrics of Korean Medicine
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• v.18 no.1
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• pp.105-129
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• 2004

Methods: It was studied on the 47 kinds of oriental medical literature for fecal blood. Results: Fecal blood means bleeding with faces from anus and indicates all the three cases such as blooding before and after evacuation, evacuation of feces mixed with blood, and simple melena. The main causes are fire(火) and deficiency of spleen qi (脾氣虛). According to the color of fecal blood and the region of the bleeding, first, if the blood color is dark-red and blood discharges after emptying the bowels, it is called deposited poison into Jang(臟毒) or distant bleeding(遠血), and if the color of blood is clear and bowel emptying occurs after bleeding, it is bloody stool due to intestinal wind(腸風) or nearby bleeding(近血). For treating methods(治法). removing heat from the blood and stopping bleeding(淸熱凉血止血), removing dampness and stopping bleeding(淸熱除濕止血), invigorating Ki for promoting Hul-controJ(益氣攝血), warming the spleen and stomach to dispel cold and stopping bleeding(溫中散寒止血) etc are applied. As for the treating prescriptionl(治法), a Hwangtotang(黃土湯). Jeoksodudanggwitang(赤小豆當歸散). Gwihwasan(槐花散). Wipungtang(胃風湯). Hwangnyeonhwan modifying(黃連丸加減). Samultang modifying(四物湯加減). Paedoksan modifying(敗毒散加減) etc are used. As for acupuncture and moxibustion(鍼灸療法). if etiology(病因) is damp-heat(濕熱), acupuncture(刺鍼) at Janggang(長强); Charyo(BL 32); Sangeoheo(ST 37)(上巨虛); Seungsan(BL 57)(承山穴), and in case of deficiency of spleen Gi(脾氣虛), acupunture(刺鍼) with tonification(補法) at I Baek(EX-UE 2)(二白); Gwanwon(CC 4)(關元); Joksamni(ST 36)(足三里) Taebaek(SP 3)(太白); Hoeeum(CC 1)(會陰穴), or mxibuston(灸) at Baekoe(GC 2O)(百會); Myeongmun9GC (命門) or the point of opposite to umbilicus among spinal vertebrae(脊中對臍穴) are used. The external treatment(外治療法) was consisted of plastering umbilicus therapy(敷臍法) and enema therapy(灌腸法).

• Journal of Acupuncture Research
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• v.24 no.1
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• pp.29-38
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• 2007

Objectives : The objective of this study was to research delayed growth with acupuncture and moxibustion treatment. Methods : We search the oriental medical literature related to delayed growth, especially loose skull, pigeon chest(龜胸), turtle back(龜背), five kinds of flaccidity(五軟), five kinds of retardations(五遲) and infantile malnutrition(疳證). Results : 1. Loose skull is treated with moxibustion therapy of CV8(神厥) and two points(1.5cm upper and under of CV8) 2. Pigeon chest(龜胸) is treated with moxibustion therapy of GB38(外丘), ST18(乳根) and 6 points around of STl7(乳中). 3. Turtle back(龜背) is treated with moxibustion therapy of BLl3(肺兪), BL15(心兪) and BLl7(膈兪) 4. Acupuncture therapy of five kinds of flaccidity(五軟) was rare. but there are one case to stimulate Hwatahyeopcheok point using plum-blossom needle. 5. Five kinds of retardations(五遲) is treated with moxibustion therapy of BLl5(心兪) and two point of medial malleolus 6. Infantile malnutrition(疳證) is treated with acupuncture therapy of the spleen channel and stomach. channel, therapy using three-edged needle, cutting therapy(LUlO(魚際) and Sabong), moxibustion therapy(LRl3(章門) and BL2l(胃兪), and Ch'una therapy. Conclusion: We expect that acupuncture and moxibustion treatment of delayed growth will be applied practically in clinical medicine due to further study on delayed growth.

• Toxicological Research
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• v.18 no.2
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• pp.205-213
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• 2002

This study was undertaken to develop a subacute murine model for predicting occurrence of systemic anaphylaxis and to evaluate efficacy of various immunological parameters as the monitoring indices for the occurrence of anaphyalxis. The murine anaphyalxis model was developed through intraperitoneally sensitizing 100 $\mu\textrm{g}$ ovalbumin (OVA) in the presence of 1 mg alum and 300 ng cholera toxin twice a week interval followed by challenging 500 $\mu\textrm{g}$. OVA intravenously. Typical anaphylaxis symptoms were demonstrated at the both BALB/c mice, a strain prone to type-2 response, and C57BL/6 mice. a strain prone to type-1 response. Level of plasma histamine was approximately 50-fold or 30-fold higher in the mice sensitized with OVA than the mice sensitized with alum plus cholera toxin or the saline-treated mice after OVA challenge, respectively. Sensitization and challenge with OVA significantly enhanced plasma leukotriene $B_4$ level but not IgE levels in comparison with the control mice, which indicated the role of leukotriene $B_4$ for progression of anaphyalxis. Furthermore, among mice suffered from anaphylaxis, levels of OVA-specific IgGl were significantly higher in the BALB/c mice than in the C57BL/6 mice, which implied the genetic susceptibility for the induction of systemic anaphylaxis. Conclusively, simultaneous evaluation of histamine, leukotriene $B_4$, and allergen-specific IgG isotype may serve as more efficient monitoring tool for vaccine-related anaphyalxis.

• KSBB Journal
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• v.25 no.1
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• pp.18-24
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• 2010

Juglans mandshurica belongs to the family Juglandaceae is known to contain a wide range of pharmacological activities including anti-cancer, anti-inflammation, astringent, and anti-human immunodeficiency virus-type 1 (HIV-1). Melanogenesis refers to the biosynthesis of melanin pigment in melanocytes. In this study, to investigate the whitening activity of the extracts from Juglans mandshurica, we measured effects on a tyrosinase activity, a melanogenesis, and a tyrosinase synthesis in the B16/BL6 melanoma cells and an antioxidant activity. The extracts significantly scavenged a 1,1-diphenyl-2-picrylhydrazyl (DPPH) and a superoxide anion radicals in a dose-dependent manner with a $SC_{50}$ value of $20\;{\mu}g/mL$ and $25\;{\mu}g/mL$, respectively. Also, the tyrosinase activity and melanogenesis were significantly inhibited by the extracts. Furthermore, the synthesis of tyrosinase protein was significantly decreased by the extracts in enzyme-linked immunosorbent assay. Double blind study on the clinical efficacy of a cream containing 2% of the extracts showed that the extracts have a significant skin whitening effect. Therefore, this study demonstrates that the extracts from Juglans mandshurica may be useful as a potential agent for skin whitening.

• Korean Journal of Acupuncture
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• v.21 no.2
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• pp.177-192
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• 2004

Objectives : This study is designed to investigate the pathology, acupuncture and moxibustion therapy applicable of the dementia through literature of oriental medicine. Results : The findings of this study are as follows ; 1. The cause of dementia is internal damage of seven emotions(七情內傷), deficiency of the kidney essence(腎精不足), retention of dampness in the mental(痰迷心竅). 2. The acupuncture meridians used often for dementia are $Tok-maek(GV),\;Chok-t'aeyang\;Pang-gwang-Ky\check{o}ng(BL),\;Chok-yangm\check{o}yng\;Wi-Ky\check{o}ng(ST),\;Su-gwor\check{u}m\;Shimp'o-Ky\check{o}ng(PC),\;Su-so\check{u}m\;Shim-Ky\check{o}ng(HT)$. 3. The spots on the body suiTable for acupuncture used for therapy of the dementia are 98 points and extra points are 6 points, total therapeutic acupunture points are 104 points. And acupunture points are frequently used Shinmun(神門-HT7, 24), Paek'oe(百會-GV20, 19), Chok-samni(足三里-ST36, 18), Sugu(水溝-GV26, 17), P'ungnyung(豊隆-ST40, 16), Taech'u(大椎-GV14, 14), $Sam\check{u}mgyo(三陰交-SP6,\;14)$, Shimsu(心兪-BL15, 14), $Taen\check{u}ng(大陵-PC7,\;11)$, Nogung(勞宮-PC8, 11), Sosang(少商-LU11, 10), Kansa(間使-PC5, 10), Naegwan(內關-PC6, 10). 4. The acupoints used often on moxibustion are Paek'oe(百會-GV20), Chang-gang(長强-GV1), Taech'u(大椎-GV14), Kansa(間使-PC5). Conclusion : It is recommended that further study of many sided investigations in the future.