• 한국염색가공학회지
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• 제8권4호
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• pp.19-24
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• 1996

Poly(3-hydroxybutylate) 및 그들 유도체의 화학적 합성을 위해 ${\gamma}$-butyrolactone(${\gamma}$BL)과 ${\gamma}$-valerolactone(${\gamma}$VL)을 사용 ${\gamma}$-butyrolactone($\beta$BL) 과의 개환중합을 시도했다. 공중합체는 5원황 락톤 단위를 포함한 코폴리에스테르를 $BF_{3}$. $OR_{t2}$ 촉매하에서 고상(bluk state)중합에 의해 얻었고, 이러한 방법으로 합성한 코폴리머의 구조를$^{1}H NMR$과 $^{13}C NMR$분석법으로 결정했다. 그결과 $\beta$BL, ${\gamma}$BL과 ${\gamma}$VL의 첨가비가 증가함에 따라 수율은 저하되었고, 또한 ${\gamma}$VL의 경우 4HV의 증가가 34~35%가 한계로써, ${\gamma}$VL의 첨가비가 0.5 (${\gamma}$VL/$\beta$VL=50/50)보다 증가 할지라도 안정상태를 유지하였다.

• 혜화의학회지
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• 제9권1호
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• pp.169-182
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• 2000

To evaluate the antitumor activity and antimetastatic effects of Bujeongyangeumtang(BJYET), studies were done experimentally. The results were obtained as follows: 1. BJYET extracts exhibited a significant cytotoxicity against A549, SK-MEL-2, SK-OV-3 and B16-BL6 cell lines. 2. The T/C% was 118.2% in BJYET treated group in S-180 bearing ICR mice. 3. BJYET extracts exhibited inefficient adhesive effect of A549, B16-BL6 cell to complex extracellular matrix. 4. BJYET extracts showed a significant inhibition of lung metastasis of B16-BL6 cells in C57BL/6. 5. In vitro neovascularization assays, angiogenesis was significantly inhibited in BJYET treated group than control group. These results suggested that BJYET extracts might be usefully applied for prevention and treatment of cancer.

• 대한한의학회지
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• 제18권1호
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• pp.326-336
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• 1997

In order to investigate the effect of aqua-acupuncture solution with Panax Ginseng into $Qihai(CV_6)$ and $Shenshu(BL_{23})$ on immune response induced by glucocorticoid in mouse, Panax Ginseng aqua-acupuncture solution was injected into $Qihai(CV_6)$ and $Shenshu(BL_{23})$ for seven days after injection with glucocorticoid. And then MA-HRP (methamphetamine-horseradish peroxidase) induced antibody production, numbers and lysozyme activity in macrophage were measured. The results were as follows: 1. The antibody production in mouse immunized with MA-HRP was decreased in control group as compared with normal group. Although $Qihai(CV_6)$ group showed slight increasement, $Shenshu(BL_{23})$ group indicated great increasement compared with normal group. However in Aa-BL group, antibody production was almost increased to normal group. 2. In control group, the numbers of macrophage were decreased about 14% as compared with normal group. And in the pretreated groups of $Qihai(CV_6)$ and $Shenshu(BL_{23})$ were respectively increased 3.0 times and 2.9 times as compared with normal group. 3. Effect of Panax Ginseng-aqua acupuncture solution on the lysozyme activity in macrophage was increased gradually in the pretreated groups of $Qihai(CV_6)$ and $Shenshu(BL_{23})$ as compared with control group. These results suggest that Panax Ginseng aqua-acupuncture at $Qihai(CV_6)$ and $Shenshu(BL_{23})$ may increase antibody production and lysozyme activity in macrophage that is suppressed by glucocorticoid, and Panax Ginseng aqua-acupuncture will have immuno adjuvant effects on the cells which concerned with immunomechanisms.

• Applied Biological Chemistry
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• 제48권3호
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• pp.201-206
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• 2005

본 연구는 한약재박의 효율적 처리를 위한 실험의 일환으로 한약재박으로부터 유용미생물을 분리하고 이들 미생물로부터 작물생육촉진미생물을 선발하여 한약재박에 적용한 한약재박 미생물제제를 만들어 친환경농업에 이용하고자 실시하였다. 한약재박은 유기물 함량이 93.0%로 높고 조단백질 함량이 11.3%, 조지방 함량이 5.1%, NDF 함량이 49.7% 및 ADF 함량이 33.8%로 나타나 미생물제제의 원료로서도 이용 가치가 높았다. 한약재박으로부터 약 35종의 미생물이 검출되었고, 이들 중 13종이 한약재박 이용성이 높았으며, 작물생육촉진미생물 BL-333 균주를 선발하였다. 선발균주 BL-333을 Bergey's Manual of Systematic Bacteriology 및 16S rDNA 염기서열(589 bp)로 상동성을 조사한 결과 Paenibacillus marcerans로 추정되었다. P. marcerans BL-333 균주는 각종 진균에 대해 대부분 높은 항진균 활성을 나타내었으며, 특히 Fusarium 속과 Collectotrichum 속 등의 진균에 대해 높은 항진균 활성을 나타내었다. P. marcerans BL-333 균주와 한약재박 분쇄물 등으로 제조한 미생물제제는 10종의 작물의 생육조사에서 무처리구보다 $3{\sim}24%$ 증수되는 효과를 나타내었으며, 특히 상추, 무, 배추 및 오이 등에서 생육촉진효과가 우수하였다. 따라서 한약재박 및 한약재박으로부터 얻은 미생물은 퇴비나 미생물제제를 위해 효과적인 재료로서의 활용 가치가 있다고 본다.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.626-632
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• 2005

A new bacterium BL-2 excreting a novel cationic polyglucosamine biopolymer was isolated from the spoiled leaves of Chinese cabbage and identified as Enterobacter sp. BL-2. The isolated Enterobacter sp. BL-2 was cultivated in pH-stat fed-batch culture using acetic acid as the feeding stock at pH 8.0, resulting in 17.11 g/l of cells and 1.53 g/l of an extracellular biopolymer after 72 h. The excreted biopolymer was purified by a three-step procedure, involving ethanol precipitation and deproteinizations, to a nearly homogeneous state, and its molecular weight was found to be 106 kDa. It was composed of glucosamine, rhamnose, and galactose at a molar ratio of 86.4:1.6:1.0, respectively, indicating a rarely found novel high-glucosamine-containing biopolymer. The FT-IR and $^{13}C-NMR$ spectra of the novel cationic polyglucosamine biopolymer PGB-l revealed a close identity with chitosan from crab shell. It can effectively flocculate various suspended solids, including kaolin clay, $Ca(OH)_2,\;Al_{2}O_3$, active carbon, microbial cells, and acidic dyes.

• Advances in pediatric surgery
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• 제3권2호
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• pp.98-107
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• 1997

To determine whether bile juice exclusion can prevent the mucosal damage, and Insulin-like growth factor-I can promote mucosal regeneration in ischemia-reperfusion injury of the bowel, 39 weanling rats with 10 cm of Thiry-Vella loop were studied. Animal groups were; Control, BL(common bile duct ligation), IGF{insulin-like growth factor-I(IGF-I) infusion} and IGF-BL(combined treatment). IGF-I(1.5 mg/kg/day) was continuously delivered through a subcutaneously implanted miniosmotic pump. After 15 minutes of superior mesenteric artery clamping, a tissue specimen(P) was taken after 30 minutes of reperfusion. Intestinal continuity was restored to allow oral feeding. A specimen of main tract(M) and another of the Thiry-Vella loop(T) were collected for histomorphometry after 48 hours of reperfusion and free feeding. Villus size ratio(VSR), crypt depth(CD), crypt-depth/villus-height ratio(CVR) and injury score(IS) were measured in 15 consecutive villi. The postoperative mortalities of bile duct ligation groups(BL and IGF-BL) were higher than those of other groups. In control group, VSR of M was lower(P<0.05) than P or T, but not in the other groups. VSR of M in control was lower than those in other groups. CD of T in control, IGF and IGF-BL group were higher than those of M. CD of M and T showed gradual increments from control, IGF and IGF-BL group, respectively. CVR of M and T in IGF group were higher than those in control. CVR in IGF-BL group, T was higher than M, and M was higher than P. About IS, M of BL($20.1{\pm}2.5$) and IGF-BL($20.9{\pm}3.3$) groups were significantly lower than that of control($32.4{\pm}2.5$). These results suggest that the exclusion of bile juice reduces the severity of the reperfusion injury of the mucosa, by inability to activate pancreatic enzymes and IGF-I stimulates mucosal regeneration in injured bowel, and the effect is potentiated by bile juice exclusion.

• 한국양식학회지
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• 제21권2호
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• pp.82-88
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• 2008

본 연구는 치어기 조피볼락 Sebastes schlegeli 사료내 생균제 첨가가 넙치의 성장 및 면역반응에 미치는 영향을 평가하였다. 실험사료는 주 단백질원으로 북양어분(white fish meal), 콘글루텐밀(corn gluten meal), 탈피대두박(dehulled soybean meal)을 사용하였으며, 탄수화물원으로는 밀가루(wheat flour)를, 지질원으로는 고도불포화지방산(n-3 HUFA)이 다량 함유된 오징어간유(squid liver oil)를 사용하였다. 생균제의 첨가효과를 확인하기 위하여 B. polyfermenticus(BP), Bacillus licheniformis(BL) 및 복합종균(B. polyfermenticus+Saccharomyces cerevisiae; BP+SC)을 실험사료 내에 각각 $1.0{\times}10^7$ CFU/kg diet 수준으로 첨가하였다. 2주간의 예비사육 후, 평균무게 $12.0{\pm}0.1g(mean{\pm}SD)$인 조피볼락을 500 L 원형수조에 각 실험구 당 각각 20마리씩 3반복으로 무작위로 배치하였고, 실험사료는 1일 2회 어체중의 $1.7{\pm}0.6%$(오전 10시, 오후 4시)씩 12주간 공급 하였다. 증체율, 사료효율, 일간성장율 및 단백질 전환효율에 있어서 BP+SC를 공급한 실험구가 대조구(control)에 비하여 유의하게 높게 나타났지만(P<0.05), BP, BL 및 BP+SC를 공급한 실험구간에는 유의한 차이가 나타나지 않았다. 생존율에 있어서는 모든 실험구간에 있어 유의한 차이가 나타나지 않았다. 전어체 지방함량에 있어서 대조구가 다른 모든 실험구에 비해 높게 나타났으며, 전어체 단백질, 회분 및 수분함량에 있어서는 모든실험구간에 유의한 차이가 없었다. 혈액분석에 있어서 혈장내 glucose의 함량은 BP+SC를 공급한 실험구가 대조구에 비해 유의적으로 낮게 나타났으나, BP, BL 및 BP+SC를 공급한 실험구간에는 유의적인 차이가 나타나지 않았다. Respiratory burst activity(NBT assay)에 있어서 BP+SC와 BL을 공급한 실험구가 대조구에 비해 유의적으로 낮게 나타났으며, BP, BL 및 BP+SC를 공급한 실험구간에는 유의적인 차이가 나타나지 않았다. 혈청의 lysozyme 활성에 있어서는 BP와 BL을 공급한 실험구가 대조구에 비해 높게 나타났으며, BP, BL 및 BP+SC를 공급한 실험구간에는 유의적인 차이가 나타나지 않았다. 공격실험 결과, 폐사는 Edwardsiella tarda를 접종한지 1일 후부터 시작하여 11일째 종료 하였다. 생균제를 투여한 모든 실험구가 대조구에 비해 초기폐사율이 낮게 나타났다. 공격실험 후 8일째부터 종료시 까지는 BP+SC를 공급한 실험구가 대조구에 비해 유의적으로 낮게 나타났으며, BP, BL및 BP+SC를 공급한 실험구간에는 유의한 차이가 나타나지 않았다. 상기 결과를 토대로, 조피볼락 사료내 B. polyfermenticus, B. licheniformis 및 B. polyfermenticus 와 S. cerevisiae의 혼합첨가는 조피볼락의 성장 및 사료효율 증진과 항산화능 및 특정질병저항성에 좋은 효과를 나타낼 수 있을 것으로 사료된다.

• 혜화의학회지
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• 제28권2호
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• pp.48-53
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• 2019

Objectives : The aim of this study was to observe the clinical effect of warming acupunture therapy at Buyang(BL59), Golyun(BL60) in a traffic accident patient with neck pain. Methods : A 32-years-old, female patient with neck pain was treated by warming acupunture therapy at Buyang(BL59), Golyun(BL60) from April 28th to May 5th. The improvement of the patient's neck pain was evaluated by Visual Analog Scale(VAS), Pain Rating Score(PRS) and Neck Disability Index(NDI). Result : After treatment, flexion & extension VAS was decreased from 7.0 to 3.0 and lateral bending VAS was decreased from 7.5 to 4.0. PRS and NDI was improved 70 to 54, 41 to 35, respectively. Conclusions : The result suggests that warming acupunture therapy at Buyang(BL59), Golyun(BL60) may have therapeutic effect on neck pain patients.

• Journal of Acupuncture Research
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• 제19권6호
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• pp.49-60
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• 2002

Objective : The aim of this experiment is to investigate the effect of acupuncture and moxibustion at SP6(Sanyinjiao) and BL28(Pangguanshu) on uranyl acetate-induced acute renal failure in rats. Methods : To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later acupuncture and moxibustion treatment was done at SP6(Sanyinjiao) and BL28(Pangguanshu) bilaterally of the rats. And then 4 days later, we measured rats' body weight, kidney weight, serum BUN, creatinine and serum eletrolyte levels(Na, K and Cl). Results : In the moxibustion group, hypertrophy index of kidney and serum BUN level were decreased marginally significantly, and serum creatinine, K levels were decreased significantly. But in the acupuncture group, there was no significance at this experiment. Conclusions : SP6(Sanyinjiao) and BL28(Pangguanshu) moxibustion can decrease serum BUN, creatinine, and serum eletrolyte levels on uranyl acetate-induced acute renal failure in rats. And the study will be done continuously about non-effect in the acupuncture group at this experiment.

• PNF and Movement
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• 제15권3호
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• pp.343-351
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• 2017

Purpose: This study aimed to suggest effective lunge exercise methods for various purposes by comparing differences in the muscle activity of thigh and plantar pressure according to changes in base of support during lunge exercises. Methods: The subjects were 20 college students who agreed to participate in the experiment. They performed three types of lunges-a basic lunge (BL), wide lunge (WL), and narrow lunge (NL). In a static position during each lunge, the muscle activity of the thigh and plantar pressure were measured. Each movement was maintained for 7 seconds, and the muscle activity for the 3 seconds in the middle was measured and analyzed. The plantar pressure was divided into six areas for analysis. The subjects randomly performed the lunges to prevent the influence of an experimental sequence. Each movement was performed three times, and the mean value of the three measurements was analyzed. Results: The analysis of muscle activities in the thigh during the three lunge movements showed statistically significantly higher muscle activity of biceps femoris in WL and NL than BL. Moreover, the analysis of plantar force showed statistically significant differences between BL and WL and WL and NL on the medial-forefoot and medial-midfoot, as well as between BL and WL and BL and NL on the lateral-midfoot. The analysis of plantar contact pressure exhibited statistically significant differences between BL and NL and WL and NL on the medial-forefoot, as well as a statistically significant difference between WL and NL on the medial-midfoot and lateral-hindfoot. Conclusion: This study suggests that changing the base of support during lunges can be useful to suit the purposes of various rehabilitation programs.