• Microbiology and Biotechnology Letters
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• v.26 no.1
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• pp.61-67
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• 1998

Recombinant human epidermal growth factor (rhEGF) was produced by E. coli BL2l (DE3) harboring a plasmid pYHB101. The production of rhEGF was 44.5 mg/L when the E. coli BL2l (pYHB101) was cultured at 27$^{\circ}C$ for 48 hr in the modified MBL medium containing 10 $\mu\textrm{g}$/L glucose with 10 $\mu\textrm{m}$ IPTG/lactose induction at 2 hr after inoculation. It was shown that lactose is able to induce the rhEGF expression of E. coli BL2l (pYHB101) with the same efficiency as IPTG. In the batch culture system, when induced with 10 $\mu\textrm{m}$ lactose, E. coli BL2l (pYHB101) produced maximum 45 mg/L of the rhEGF at 28 hr culture in the modified MBL medium containing 10 g/L glucose. In the semi-fed batch culture system, the volumetric yield was 160 mg/L when the culture was added with 0.5% (w/v) lactose and 0.25% (w/v) yeast extract in the late logarithmic phase and 94.3% of rhEGF was secreted as soluble form. However, when the culture was added with them in the early logarithmic phase, the volumetric yield was 120 mg/L and 20.9% of rhEGF was found in cytoplasmic insoluble aggregates. It was found that the addition time of lactose was important for production of soluble rhEGF from E. coli BL21 (pYHB101).

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.149-153
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• 2003

This study was conducted to survey the utilization possibility of composting system of small scale static pile with animal manure produced from cattle shed and the effect of addition of microorganism on the maturity of compost. Microorganisms added in composting substrate were bacteria+lactobacillus(BL)+photo.(BLP). The composting practiced was a windrow system without aeration equipment and turning was practiced periodically. The water content of substrate mixed with cow manure, rice husk, and sawdust was about 60%. The temperature during the composting process was increased at over $60^{\circ}C$ within 3 days after composting starting. Increase of temperature at the early stage of composting was fasten in BLP and BL than Control. Because the pH of the raw material was high, the changes of pH during composting was little and stabilized in weak alkaline condition. EC value was high for accumulation of manure and urine excreted continuously by animal and the changes of those during composting occurred in 5~10% increase. Reduction rates of C/N ratio were the largest as the 22.7% in BLP and 19.2and 17.5% in BL and Control respectively. In the evaluation of phytotoxicity, there was stabilized within the short time in BLP and not the difference between BL and Control. Treatment of animal manure produced from small scale cattle shed was possible by using the small scale static pile composting system with reasonable water content and turning and the addition of microorganism in composting substrate was effected on the temperature increase at the early stage of composting and reduction of plant toxicity compounds but little on the maturity of compost.

• Journal of Oriental Neuropsychiatry
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• v.24 no.3
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• pp.263-270
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• 2013

Objectives : Galgunhaejutang extract (GHT) is a Traditional Korean medical herbal mixture that has been tradtionally used to treat alcohol consumption. This study was preformed to evaluate the effects of GHT on alcohol consumption in C57BL/6 mice. Methods : Sixty three C57BL/6 mice were alcohol dependence-induced by limited access paradigm. Water, GHT 0.688 g/kg (GHT-L), or GHT 3.45 g/kg (GHT-H) were administrated for 10 days. The amounts of alcohol consumption for 2 hours, water consumption for 22 hours, food intake for 24 hours and body weight were measured. Results : There weren't significant differences in 2 hours of alcohol consumption, 22 hours of water consumption, 24 hours of food intake and body weight for ten days between vehicle group and GHT-L or GHT-H group. Conclusions : Further studies employing multi-dose and long term administration of GHT (more than 10 days) might be of benefit.

• Journal of Acupuncture Research
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• v.19 no.2
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• pp.78-91
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• 2002

Objective : This study was designed to investigate the anti-cancer effects of bee venom on melanoma in C57BL mice. Materials and Methods : For the induction of melanoma, C57BL mice were treated by DMBA(7, 12-dimethylbenz[a]anthracene). Each group of C57BL mouse was treated with DMBA $50{\mu}g$, $75{\mu}g$, $100{\mu}g$ respectively once a week for 15 weeks. Tumor generation in each group of 10 mice was observed. Cumulative curves were showed in the density and frequency of skin tumor generation. To know the effects of pre-treatment of bee venom on tumor generation by DMBA treatment(frequency of tumor generation), Each group of C57BL mouse was pretreated and treated with bee venom $5{\mu}{\ell}$, $25{\mu}{\ell}$, $50{\mu}{\ell}$ respectively once a week for 3 weeks, whereafter each mouse was treated with DMBA $100{\mu}g$ once a week for 15 weeks. Results and Conclusion (1) There was chemotherapeutic effect, but not chemopreventive effect. (2) Cpp32 activity was increased by $50{\mu}{\ell}$ bee venom treatment. (3) Bee venom treatment inhibited expression of cell-cycle regulating, growth-promoting genes such as c-Jun, c-Fos, and Cyclin Dl, and increased tumor suppressors p53 and p21/Wafl. (4) Bee venom treatment activated expression of a representative apoptosis-inducing gene Bax.

• Journal of Acupuncture Research
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• v.31 no.3
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• pp.91-102
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• 2014

Objectives : This study was done to determine the effect of Korean medical treatment to patient diagnosed as cauda equina syndrome(CES) suffering from both lower limb dysesthesia, saddle dysesthesia, buttock dysesthesia, dysuria, dyschezia and gait disturbance. Methods : A patient with CES was treated with acupuncture, sweet bee venom pharmacopuncture, herbal medicine from February 2 to April 9 at Department of Acupuncture & Moxibustion, Daejeon Korean Hospital of Daejeon University. Patient received acupuncture treatment($CV_1$, $CV_3$, $CV_4$, $CV_6$, $ST_{36}$, $BL_{31}$, $BL_{32}$, $BL_{33}$, $BL_{34}$) 12 times a week, bee-venom pharmacopuncture($BL_{23}$, $BL_{28}$) was conducted twice a week, herbal medicine(Samilsingihwan decoction) was applied to patient thrice a day for nine weeks. The following symptoms were observed saddle, and lower limb dysesthesia, dysuria, dyschezia and gait disturbance. Results : After treatment, dysesthesia, dysuria, dyschezia and gaiting developed good outcome. But anal reflex remained still. Conclusions : Acupuncture, bee-venom pharmacopuncture, herbal medicine combined treatment might be effective to patients with CES.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.1
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• pp.94-103
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• 2022

As a large amount of data generated by sensors or devices at the manufacturing site is transmitted to the server or client, problems arise in network processing time delay and storage resource cost increase. To solve this problem, considering the manufacturing site, where real-time responsiveness and non-disruptive processes are essential, QRC (Quotient Remainder Compression) and BL_beta compression algorithms that enable real-time and lossless compression were applied to actual manufacturing site sensor data for the first time. As a result of the experiment, BL_beta had a higher compression rate than QRC. As a result of experimenting with the same data by slightly adjusting the data size of QRC, the compression rate of the QRC algorithm with the adjusted data size was 35.48% and 20.3% higher than the existing QRC and BL_beta compression algorithms.

• ALGAE
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• v.39 no.1
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• pp.1-16
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• 2024

Many dinoflagellates produce bioluminescence. To estimate the intensity of bioluminescence produced by populations of the heterotrophic dinoflagellates Noctiluca scintillans and Polykrikos kofoidii and autotrophic dinoflagellate Alexandrium mediterraneum in Korean waters, we measured cellular bioluminescence intensity as a function of water temperature and calculated population bioluminescence intensity with cell abundances and water temperature. The mean 200-second-integrated bioluminescence intensity per cell (BL_cell) of N. scintillans satiated with the chlorophyte Dunaliella salina decreased continuously with increasing water temperature from 5 to 25℃. However, the BL_cell of P. kofoidii satiated with the mixotrophic dinoflagellate Alexandrium minutum continuously increased from 5 to 15℃ but decreased at temperatures exceeding this (to 30℃). Similarly, the BL_cell of A. mediterraneum continuously increased from 10 to 20℃ but decreased between 20 and 30℃. The difference between highest and lowest BL_cell of N. scintillans, P. kofoidii, and A. mediterraneum at the tested water temperatures was 3.5, 11.8, and 21.0 times, respectively, indicating that water temperature clearly affected BL_cell. The highest estimated population bioluminescence intensity (BL_popul) of N. scintillans in Korean waters in 1998-2022 was 4.22 × 10¹³ relative light unit per liter (RLU L^-1), which was 1,850 and 554,000 times greater than that of P. kofoidii and A. mediterraneum, respectively. This indicates that N. scintillans populations produced much brighter bioluminescence in Korean waters than the populations of P. kofoidii or A. mediterraneum.

• Textile Coloration and Finishing
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• v.11 no.1
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• pp.16-24
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• 1999

The effects of two-step fixation of steaming and baking on the dischargeability of cotton fabrics dyed with C.I. Reactive Black 5(Bl-5) were investigated when the concentrations of $K_2CO_3$ and benzaldehyde sodium bisulfite(BASB) were increased over 120/kg. Remarkably increased dischargeability resulted from baking for 3 min or more at 160t after steaming for 8 min or more at $102^\circ{C}$, but 120g/kg or more amounts of $K_2CO_3$ and BASB(50%) had little influence on dischargeability. Therefore the discharge mechanism can be suggested that covalent bonds between cellulose and Bl-5 undergo $S_N2$ attack by hydroxide ion formed by the reaction of $K_2CO_3$ and water in steaming at $102^\circ{C}$ first and then, through transition states they are cleavaged in baking at 160t to yield hydrolyzed Bl-S and compounds of BASB and Bl-5 isolated from fiber, which are undyeable and removed by washing. The effect of urea, one of the hydrotrope agents, on discharge printing was also studied. The result which dischargeability was greatly improved by increasing the steaming time from 8 min to 15 min at $102^\circ{C}$ or by increasing the amount of urea obviously shows that water in steaming and urea in print paste play an important role in discharge printing. And as an increase of the baking time from 5 min to 7 min at $160^\circ{C}$ makes it possible to improve dischargeability, it is once more confirmed that high temperature of about 160t is exactly required to discharge the dyed Bl-5. The colored discharge printing demands a more amount of urea because urea contributes to the putting color fixation as well as the discharge reaction.

• Journal of the Korean housing association
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• v.5 no.2
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• pp.141-148
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• 1994

The behavioral-related environmental study for multifamily housing is needed to develop the quality of unit floor plan in terms of user comfort and needs. So, the aim of this study is to find out the living style and housing needs in terms of interaction between the users behavior pattern and orgarnization of physical home space. For the purpose, the field experiments in Pusan, have been carried out with 318 households living in apartments by plan types(2BL, 3BL, 4BL, 5BL) which were classified by number of bedrooms. The dwelling space usage behavior(room use pattern and furniture uasge pattern) and the space adjustment behavior(physical modification of space and preferred space adjustment attitude) were examined and analyzed.

• Korean Journal of Veterinary Pathology
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• v.3 no.2
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• pp.93-94
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• 1999

Spontaneous hydronephrosis was found in a 19 month-old female C57BL/KsJ-Lep $r^{db}$ / Lep $r^{db}$ mouse. We described the gross and histological appearance of spontaneous in db mouse The left kidney was dilated and filled with urine. Microscopically the renal pelvis was remarkedly dilated and the renal cortex and renal papilla were flattened.