• Journal of Life Science
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• v.18 no.2
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• pp.264-272
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• 2008

The lactate dehydrogenase (EC 1.1.1.27, LDH) isozymes in tissues from Acanthogobius hasta were characterized by biochemical, immunochemical and kinetic methods. The activities of LDH in skeletal muscle and eye tissues were 65.30 and 53.25 units, but LDH activities in heart and liver tissues were very low. LDH/CS (EC 4.1.3.7, citrate synthase) in skeletal muscle was the highest as 22.29. Specific activities of LDH in brain, eye and skeletal muscle were 56.45, 38.04 and 11.0 units/mg, respectively. The LDH isozymes in tissues were separated by polyacrylamide gel electrophoresis after immunoprecipitation with antiserum against $A_4,\;B_4$ eye-specific $C_4$ and liver-specific $C_4$. LDH $AC_4$ isozymes were detected predominantly in skeletal muscle, brain and eye tissues, and $B_4$ isozyme was detected in heart. Anodal eye-specific $C_4$ and cathodal liver-specific $C_4$ were coexpressed in A. hasta. The eye-specific $C_4$ isozyme showed higher activity in eye tissue, but liver-specific $C_4$ isozyme showed lower activity in liver. As a result, one part of molecular structures in $A_4\;and\;C_4,\;A_4\;and\;B_4$, and eye-specific $C_4$ and liver-specific $C_4$ were similar, but in $B_4\;and\;C_4$ were different with each other. Therefore the subunit A may be conservative in evolution, and the evolution of subunit B seems to be faster than that of subunit A. The LDH $A_4$ isozyme of skeletal muscle was purified in the fraction from elution with NAD+ containing buffer of affinity chromatography and eye-specific $C_4$ isozyme was eluted right after $A_4$, so the structure of eye-specific $C_4$ isozyme is similar to $A_4$. And LDH activity remained 35.22-43.47% as a result of the inhibition by pyruvate, the Michaelis-Menten constant values for pyruvate was 0.080-0.098 mM, and Vmax were 153.85 units, 35.09 units in skeletal muscle and eye, respectively. Also the $B_4$ isozyme was the thermo-stablest and $C_4$ was stabler than $A_4$ isozyme. The optimum pH of LDH was 6.5. The results mentioned above indicate that isozymes in tissues showed the properties between LDH $A_4\;and\;B_4$ isozyme as A. hasta was adapted to hypoxic conditions. Also LDH seems to function more effectively under anaerobic condition because LDH in skeletal muscle and eye tissues have high affinity for pyruvate.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.5
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• pp.815-820
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• 2008

Recently the frequency assignment and the technical specifications of UWB systems for communications are completed. Therefore many UWB systems have been developed. In our country $3.1{\sim}4.8GHz$ and $7.2{\sim}10.2GHz$ are assigned for UWB systems for communications. When we consider RF technologies and the easy implementation of UWB systems, UWB systems used in the low band are more developed than high band systems. In this paper we design and implement a BPF for low band UWB systems by means of considering the easy implementation of UWB systems. The designed and implemented BPFs are low band filter and low band channel filters. The measured results of the low band filter show that the filter has 21.85dB and 17.91dB attenuation at 3.1GHz and 4.8GHz, 1.53GHz of -10dB bandwidth and 2dB of insertion loss. Low band can be divided into 3 channels with 500MHz of the channel bandwidth. The channel filter for channel number 1 has the characteristics of 24.85dB attenuation at 3.1GHz, 0.61GHz of -10dB bandwidth and 1.87dB of insertion loss. The filter for channel 3 in low band has 19.2dB of attenuation at 4.8GHz, 0.49GHz of -10dB bandwidth and 2.49dB of insertion loss.

• Archives of Pharmacal Research
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• v.26 no.5
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• pp.358-366
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• 2003

Some N-p-substituted phenyl uracil-5-sulphonamide derivatives have been synthesized to be evaluated as molluscicides against Biomphalaria alexandrina snails, the intermediate host of Schistosoma mansoni. Schistosoma mansoni infected mice were treated with hemolymph obtained from pre-treated Biomphalaria alexandrina snails with the products 4a, 10a, 10b and 4b or obtained from non-treated snails. The selection of the concentration based on the predetermined dose which caused mortality of less than 50% of snails/24 h. $LC_{50}$ of compounds 4a, 10a, 10b and 4b was 50, 100, 200 and 50 ppm respectively. The result showed that immuno-stimulatory effect of treated hemolymph with compounds 4a, 10a and 4b was related to significant protective effects (44.4, 34.6 and 50.4% reduction in worm burden respectively). In addition, mean total worm burdens were significantly reduced in non treated hemolymph by 33.8%. The effect of hemolymph obtained from treated or non treated snails on S. mansoni adult worms antigens was studied by indirect immunofluorescence technique using chronic mouse sera (CMS). The results indicated that there was a strong reaction with epitopes in gut epithelium, tubercles, teigument and subtegumental musculature of untreated and treated S. mansoni adult worms antigens. Therefore, treatment of hemolymph obtained from pre-treated snails with compounds 4a, 10a, and 4b can stimulate specific immune response and induce protective effects against S. mansoni infection.

• Korean Journal of Materials Research
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• v.10 no.1
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• pp.49-54
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• 2000

The new green $Al_3GdB_4O_{12}:Tb^{3+} and red Al_3GdB_4O_{12}:Eu_{3+}$ phosphors were synthesized and then characterized their optical properties for PDP application. And also the photoluminescence properties of these phosphors were compared with the commercial green $Zn_2SiO_4:Mn^{2+} and (Y,Gd)BO_3: Eu^{3+}$ red PDP phosphors. The phosphors were synthesized by solid state reaction at 115$0^{\circ}C$ for 4hr. It was found that the emitting brightness of $Al_3GdB_4O_{12}:Tb^{3+}$(15mol%) green phosphor under 147nm excitation was higher than that of commercial $Zn_2SiO_4: Mn^{2+}$ green PDP phosphor. However, the color coordinate of this new green phosphor was inferior to the commercial one. On the other hand, the emitting intensity of $Al_3GdB_4O_{12}:Eu^{3+}$(15mol%) red phosphor was smaller than the $commercial(Y,Gd)BO_3: Eu^{3+}$ red one, but the CIE coordinate was slightly improved. The excitation spectrum showed that $Al_3GdB_4O_{12}$ phosphors had a strong excitation band at $\lambda=160nm$ associated with the host absorption. And the photoluminance excitation (PLE) intensity in VUV range for $Al_3GdB_4O_{12}:Tb^{3+}$ green phosphor was higher than that of $Zn_2SiO_4: Mn^{2+}$, but the PLE intensity of $Al_3GdB_4O_{12}:Eu^{3+}$ red phosphor was smaller than $(Y,Gd)BO_3: Eu^{3+}$.

• Biomolecules & Therapeutics
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• v.9 no.3
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• pp.162-166
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• 2001

Helicobacter pylori is a major cause of gastric-associated diseases. In our previous study, the Adhesin/CTXA2B was expressed as insoluble recombinant chimeric protein derived from the H. pylori adhesin genetically coupled to CTXA2B subunit in Escherichia coli. Since it is very important to optimize IPTG concentration, culture temperature and composition of medium to maximize cell growth and productivity, these conditional growth factors were determined for increasing the productivity of the expressed Adhesin/CTXA2B chimeric protein in Escherichia coli JM101 carrying pTEDhpa/ctxa2b. Our data demonstrate that optimal medium for increased production of chimeric protein was a YCP/Glu medium composed of 2% yeast extract, 1% casamino acid, phosphate solution [0.3% $KH_2P0_4$, 0.4% $Na_2HP0_4$, 0.25% ($NH_4)_2HPO_4$], and 0.5% glucose. In addition, optimal concentration of IPTG was 1 mM and culture temperature, $37^{\circ}C$.

• Journal of Magnetics
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• v.14 no.4
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• pp.150-154
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• 2009

The effect of Ga and, Nb addition on the kinetics and mechanism of the disproportionation of a Nd-Fe-B alloy were investigated by isothermal thermopiezic analysis (TPA) using $Nd_{12.5}Fe_{(81.1-(x+y))}B_{6.4}Ga_xNb_y$ (x=0 and 0.3, y= 0 and 0.2) alloys. The addition of Ga and Nb retarded the disproportionation kinetics of the Nd-Fe-B alloy significantly, and increased the activation energy of the disproportionation reaction. The disproportionation kinetics of the $Nd_{12.5}Fe_{(81.1-(x+y))}B_{6.4}Ga_xNb_y$ alloys measured under an initial hydrogen pressure of 0.02 MPa were fitted to a parabolic rate law. This suggested that during the disproportionation of $Nd_{12.5}Fe_{(81.1-(x+y))}B_{6.4}Ga_xNb_y$ alloys with an initial hydrogen pressure of 0.02 MPa, a continuous disproportionation product is formed and the overall reaction rate is limited by the diffusion of hydrogen atoms (or ions).

• Journal of information and communication convergence engineering
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• v.7 no.3
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• pp.253-257
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• 2009

As the value of B2B integration gets more importance and the business process becomes more complex, BPEL4WS standard has been focused for the solution of the problems which is based on web service technology. Many vendors implement the standard in their systems to integrate B2B and web service based inner-Enterprise Application(EA)s. But there is no way to use the standard to integrate non-web service based EAs, because the standard is designed only for Service Oriented Architecture (SOA) over web services. This paper proposes Hybrid Service Orchestration Framework to participate web services and also non-web service based EAs in the business process as a partner using BPEL4WS standard. The framework introduces Hybrid Service Architecture to represent non-web service based EAs as a service, so BPEL4WS could be used to integrate web services and non-web service based EAs. And the framework also introduces Hybrid Service Toolkit to support software level facilities. The paper also introduces Hybrid Orchestration System that is based on Hybrid Service Orchestration Framework.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.5
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• pp.748-754
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• 2000

A novel single feed wide band CP stacked microstrip antenna using crossed slots has been designed, fabricated and measured. For the single rediating element the designed 10dB return loss bandwidth is 34.5%99.45~13.54 GHz), 3dB axial ratio bandwidth is 18.7%(11.17~13.39GHz), and 6 dB gain bandwidth is 29%(10.21~13.64GHz). For the 2$\times$2 array designed using a sequential rotation method, the 10dB return loss bandwidth is 35.9%(9.69~13.94GHz), 3dB axial ratio bandwidth is 34.6GHz (9.93~14.03GHz), and 6dB gain bandwidth is 27.4%(10.35~13.6GHz). For the fabricated 8$\times$8 array antenna, the 10dB return loss bandwidth is 27.3%(10.17~13.41GHz), 3dB axial ratio bandwidth is 27.9GHz(10.1~13.4GHz), and the radiation pattern is good agreement with theory. This antenna can be used for broadband applications for communications or broadcasting in Ku band.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.413-419
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• 2011

This study was conducted to determine the physicochemical properties of a giant embryo rice 'P47JB-4-B-5-B' derived from the cross between 'P47', a mutant of 'Hwayoung' induced by T-DNA insertion, and 'Junam'. The grain appearance and chemical components of the embryo were analyzed and compared with a donor cultivar, 'Hwayoung'. The proportion of embryo weight to grain weight of 'P47 JB-4-B-5-B' was 2.2 times heavier (6.7%) than that (3.1%) of 'Hwayoung'. Total free amino acid content (75.81 mg/100 g) of 'P47JB-4-B-5-B' was 2.1 times higher than that of 'Hwayoung'. The GABA content in brown rice was 14.06 mg/100 g in 'P47JB-4-B-5-B' and 6.8 mg/100 g in 'Hwayoung'. Especially, the GABA content in brown rice of 'P47JB-4-B-5-B' remarkably increased (about 33 times from 1.48 mg to 44.81 mg/100 g) 2 days after germination. Continuous frequency distributions and transgressive segregation in embryo length and width were observed in the $F_2$ population of the cross between 'P47' and 'Cheongcheong', indicating that the giant embryo was controlled by quantitative trait loci. However, embryo length and width demonstrated high broad sense heritability, implying that giant embryonic traits could be selected in earlier generations in comparison with other quantitative traits.

• The Korean Journal of Ceramics
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• v.5 no.4
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• pp.324-330
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• 1999

$Si_3N_4$-$TiB_2$ with 2 wt% $Al_2O_3$ and 4 wt% $Y_2O_3$ additives was hot pressed in a flowing $N_2$ environment with varying $TiB_2$ content from 10 to 50 vol%. Variations of mechanical (hardness, fracture toughness, and flexual strength), and tribological properties as a function of $TiB_2$ content were investigated. As the content of $TiB_2$ increased, relative density decreased due to the chemical reaction of $TiB_2$in $N_2$ environment. The reduction of density causes mechanical properties to be degraded with an increase of $TiB_2$ in $Si_3N_4$. Tribological properties were dependent of microstructure as well as mechanical properties, however, they were degraded strongly by the chemical reaction of $Si_3N_4$-$TiB_2$ during hot pressing in $N_2$ environment. SEM and TEM observations, and X-ray diffraction analysis that the chemical reaction products at the interface are TiCN, Si, and $SiO_2$. Also, the comparison of XRD patterns of the $Si_3N_4$-40 vol% $TiB_2$ composites hot pressed at $1,750^{\circ}C$ for 1 hour between in $N_2$ and in Ar gas was made. The XRD peaks of Si and $SiO_2$ were not found in Ar, but still a weak peak of TiCN was presented.