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Revision of Structures of Flavanoids from Scutellaria indica and Their Protein Tyrosine Phosphatase 1B Inhibitory Activity

  • Min, Byung-Sun
    • Natural Product Sciences
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    • v.12 no.4
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    • pp.205-209
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    • 2006
  • The structures of flavonoids, 2(S)-5,7-dihydroxy-8,2'-dimethoxyflavanone (1), wogonin (2), 2(S)-5,7, 2'-trihydroxy-8-methoxyflavanone (3), and 2(S)-5,2',5'-trihydroxy-7,8-dimethoxyflavanone (4), isolated from Scutellaria indica were revised on the basis of 2D NMR spectroscopy, including to gCOSY, gHSQC, and gHMBC. Compounds 1-4 were tested in vitro protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Compounds 2 and 4 exhibited weak PTP1B inhibitory activity with $IC_{50}$ values of 208 and $337{\mu}M$, respectively.

Changes of the Boron Fraction in Soil by the Boron Application (붕소시용(硼素施用)이 토양붕소(土壤硼素)의 형태별(形態別) 함량변화(含量變化)에 미치는 영향(影響))

  • Hwang, Ki-Sung;Yoon, Jung-Hui;Park, Yong-Dae;Ho, Qyo-Soon
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.4
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    • pp.272-277
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    • 1991
  • To investigate changes of boron fractions in soil with vegetable cultivation affected by boron application Jossangmiho, chinese cabbage was planted in 1/2.000a pot with four different soils which are sandy loam, calcareous, organic and vinyl house soil. Increment of available boron in soil with different boron applications was in the order of vinyl hous soil>organic soil>sandy soil>calcareous soil. The ratio of boron fraction to total boron is highest in $NH_4$-oxalate ext.-B with 19.1 % and lowest in water sol.-B and $CaCl_2$ ext.-B with 0.7% respectively. The boron fraction increment due to the different application ratio was in the order of $NH_4-oxalate$ ext.-B>Mannitol exch.-B> $CaCl_2$ ext.-B> $NH_2OH{\cdpt}HCl$ ext.-B>Water soil.-B. As the concentration of calcium in soil increased. water sol.-B decreased and the other forms of boron increased. In the soil high in organic matter, water sol.-B and $NH_4$-oxalate ext.-B increased significantly. but the other forms of boron not.

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A Study of an 8-b*8-b adiabatic pipelined multiplier with simplified supply clock generator (단열 회로를 이용한 8-b*8-b 파이프라인 승산기와 개선된 전원 클럭 발생기의 연구)

  • Mun, Yong
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.38 no.4
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    • pp.43-43
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    • 2001
  • 단열회로를 이용한 8-b×8-b 파이프라인 승산기와 4가지 위상을 가지는 전원클럭을 공급하기 위한 개선 된 구조의 전원클럭 발생기를 설계하였다. 전원클럭 신호선의 전하는 복원되어 에너지 소모를 줄인다. 단열회로는 ECRL 형태를 기본으로 하였으며 0.6㎛ CMOS 공정을 사용하여 설계하였다. 개선된 전원클럭 발생기는 기존회로보다 4∼11% 정도 효율이 높았다. 모의실험결과 제안하는 단열회로 승산기는 CMOS 승산기보다 2.6∼3.5배 정도의 에너지를 감소시켰다.

Structural nature of chemically inequivalent borons in the nonlinear optical material β-BaB2O4 studied using 11B MAS NMR and 11B single-crystal NMR

  • Kim, Woo Young;Lim, Ae Ran
    • Journal of the Korean Magnetic Resonance Society
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    • v.17 no.2
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    • pp.86-91
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    • 2013
  • Detailed information about the structural nature of three-coordinate borons in ${\beta}-BaB_2O_4$ is obtained through $^{11}B$ MAS NMR and $^{11}B$ single-crystal NMR. The three-coordinate $BO_3$ of the two borons B(1) and B(2) in ${\beta}-BaB_2O_4$ were distinguished. The spin-lattice relaxation time in the laboratory frame $T_1$ for B(1) and B(2) slowly decreases with increasing temperature, whereas the spin-lattice relaxation time in the rotating frame $T_{1{\rho}}$ for B(1) and B(2), which differs from $T_1$, is nearly constant. The B(1) and B(2) of the two types were distinguished by $^{11}B$ MAS NMR and $^{11}B$ single-crystal NMR.

Tetraploidy Induction of Mouse Embryos by In Vitro Culture with Cytochalasin B (Cytochalasin B를 이용한 체외배양에 의한 생쥐 수정란의 4배체 유도)

  • 진동일
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.89-92
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    • 1999
  • 효율적인 homozygous 동물을 생산하기 위한 실험의 단계로 염색체가 4배체인 수정란의 이용성을 타진하기 위해 생쥐 수정란과 cytochalasin B를 사용하여 4배체 유도에 관한실험을 수행하였다. 생쥐 2-세포기 수정란을 10$\mu\textrm{g}$/ml 농도의 cytochasin B로 약 20시간 배양하였을 때 모든 수정란은 발육을 거의 멈추었으나, 이 수정란을 cytochalasin B-free medium에 체외배양하였을 때 발육이 재개되어 48시간 후 상실기나 배반포기까지 약 74%의 발육율을 나타내었다. 그러나 발육된 수정란의 세포수는 대조구에 비해 휠신 적은 것으로 나타났다. 염색체 분석결과 cytochalasin B로 처리한 대부분의 수정란은 4배체인 것으로 나타났고 약간의 수정란은 mosaicism과 다배체를 나타내기도 하였다. 그러므로 cytochalasin B를 이용하여 효과적으로 4배체의 수정란을 유도할 수 있는 것으로 나타났다.

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Synthesis of some pyridinethione derivatives and their biological activity

  • Miky, Jehane A.A.;Zahkoug, Samir A.M.
    • Natural Product Sciences
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    • v.3 no.2
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    • pp.89-99
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    • 1997
  • Aminolysis, hydrazinolysis and alkylation of 4-methoxy and 4,9-dimethoxy-6-cyano-7-thione-5-methyl-7H furo [3,2-g] [1] benzopyridine (1 a-b) yielded 7N-substituted furobenzopyridine derivatives (2 a-e or the possible isomers 3 a-e and 4 a-b), (5 a,b and 6 a,b) and the ester (8 a,b). Hydrolysis of (la) with acetic acid gave the corresponding pyridone derivatives (7). Furobenzopyridinyl-7-thioacetyl hydrazide (9 a,b) have been prepared via alkylation of furobenzopyridine thione (1 a-b) with ethyl chloroacetate followed by condensation with hydrazine hydrate. Schiff base (11) was prepared by reacting (9a) with p. N,N-dimethyl aminobenzaldehyde in boiling ethanol. Treatment of (8a) with anthranilic acid gave the corresponding 7-substituted-4H-3,1-benzoxazine-4-one (10). We found that compound (11) increased bleeding, coagulating time, the total count of white blood cells, blood glucose level (cause hyperglycemia), enzymes (GOT, GPT) activities, concentration of urea and creatinine. On the other hand it decreased red blood cells number, haemoglobin content and haematocrite value.

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Economic Performance Test of Commercial Chickens (CC) Crossbred with Parent Stock (PS) of Korean Native Chicken (KNC) (토종 부모계통 종계 간 교배 실용계의 경제능력 검정)

  • Cha, Jae-Beom;Hong, Eui-Chul;Kim, Sang-Ho;Kim, Chong-Dae;Heo, Kang-Nyeong;Choo, Hyo-Jun;Oh, Ki-Seok;Kang, Bo-Seok
    • Korean Journal of Poultry Science
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    • v.43 no.4
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    • pp.207-212
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    • 2016
  • This work was carried out to investigate the ability of thirteen crossbreds for production of commercial chicken (CC) of Korean native chicken to be cut out for Korean circumstance. Total of one thousand three hundred of crossbreds were divided thirteen groups (100 birds/group) that were twelve groups (1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B) to crossbreed with two sires (A, B) and six dams (1, 2, 3, 4, 5, 6) of parent stock (PS) of Korean native chicken and one group (HH) as commercial Korean native chicken. Experimental diets were starter diet (0~7d; CP 22.0% ME 3,150 kcal/kg), early diet (8d~Wt 800 g; CP 19.0% ME 3,150 kcal/kg) and finished diet (Wt 800 g~12 wk; CP 18.0% ME 3,150 kcal/kg). Fertility of 1B group was higher than other groups. Hatchability per fertilization eggs and hatchability per setting eggs were 87.5~89% and 87.9~96.3%, respectively, and those of 4B and 6B groups were lower compared to other groups. Weekly body weight of HH group was lower than other groups. CV values of body weight of thirteen groups were showed over 12% as 12.5~18.2%, and especially, those of 2B, 3B, 5B, HH groups were lower compared to other groups. Weekly feed conversion ratios (FCRs) of 13 crossbreds were 1.7~2.3, 2.1~2.5, 2.4~2.7 and 2.7~3.2 at the age of 6, 8, 10 and 12 weeks, respectively. Weekly production indexes of 2A, 3A and HH groups were the lower than other groups and those of thirteen groups decreased following weeks. Feed costs per body weight gain (kg) were 1,069~1,255 won, 1,100~1,263 won, and 1.468~1,696 won at starter, earlier and finished period of broilers. These results leaded down to conclusion to be possible selection of new Korean native commercial chicken that have the excellent performance and economics.

Performance Analysis of Turbo code using Semi Random Interleaver over the Radio Communication Channel (무선통신 채널에서 세미 랜덤 인터리버를 이용한 터보코드의 성능분석)

  • 홍성원
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.5
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    • pp.1067-1075
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    • 2000
  • In this paper, the performance of turbo code using semi random interleaver over the radio communication channel was analyzed. In the result, we proved that the performance of decoder was excellent as increase the interleaver size, constraint length, and iteration number. When the constraint length of turbo code using seim random interleaver and convolutional code is constant, and BER $10^{-4}$ each value of $E_b/N_o$ was 6.4(DB) and 1.7(dB). There(ore, when the constraint length was constant, we proved that the performance of turbo code using semi random interleaver is superior to convolutional code about 4.7(dB) in the case of BER=$10^{-4}$.

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Characterization of the recombinant cellulase B from Thermotoga maritima (Thermotoga maritima 유래 내열성 cellulase B 융합단백질의 특성 규명)

  • Chung Ho Kim
    • Journal of Applied Biological Chemistry
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    • v.65 no.4
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    • pp.383-386
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    • 2022
  • A gene encoding thermostable cellulase B (TmCelB) was isolated from Thermotoga maritima. The open reading frame (ORF) of TmCelB gene was 825bp long which predicted to encode 274 amino acid residues with a molecular weight of 31,732 Da. The 17 amino acid residues from N-terminal of the TmCelB was known as signal peptides. To analyze the enzymatic activity and biochemical properties, the ORF of TmCelB gene excluding a putative signal sequence encoding 17 amino acids were introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. The optimum temperature of recombinant TmCelB was around 95 ℃, and the optimum pH of recombinant TmCelB was around pH 4.5. The recombinant TmCelB was stable at temperature below 100 ℃.