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Effect of B-ring -OH numbers of 5,7-dihydroxyflavone on the activity of CYP 1 enzymes

  • Lee, Sang-Bum;Kim, Hyun-Jung;Kim, Hwan-Mook;Park, Young-In;Dong, Mi-Sook
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.112.2-112.2
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    • 2003
  • CYP1 enzymes, CYP1A1, CYP1A2 and CYP1B1, are known to bioactivate procarcinogens particularly polyaromatic compounds. Flavonoids are a class of natural compounds that are present in edible plants. Structurally, these compounds are polyphenols with two aromatic rings (A, B) and a heterocycyclic ring (C). We observed the differential inhibition of 5,7-dihydroxyflavones which are different in numbers of B-ring-OH, to the activity of ethoxyresorufin O-deethylase (EROD) in human hepatic microsomes with the IC50 values, ie, 0.57 mM, 1.28 mM, and 3.62 mM, chrysin, apigenin, and Luteolin, respectively. (omitted)

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A Property of Borel Subsets of Wiener Space

  • Ryu, K.S.
    • Journal of the Chungcheong Mathematical Society
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    • v.4 no.1
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    • pp.45-48
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    • 1991
  • Wiener measure $m({\lambda}B)$ can behave arbitrarily badly as a function of ${\lambda}$ for Wiener measurable sets B. We show however that $m({\lambda}B)$ is Borel measurable with respect to ${\lambda}$ for any Borel subset B of $C_0$[0, 1].

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Biochemical Analysis on the Parallel Pathways of Methionine Biosynthesis in Corynebacterium glutamicum

  • Hwang, Byung-Joon;Park, Soo-Dong;Kim, Youn-Hee;Kim, Pil;Lee, Heung-Shick
    • Journal of Microbiology and Biotechnology
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    • v.17 no.6
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    • pp.1010-1017
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    • 2007
  • Two alternative pathways for methionine biosynthesis are known in Corynebacterium glutamicum: one involving transsulfuration (mediated by metB and metC) and the other involving direct sulfhydrylation (mediated by metY). In this study, MetB (cystathionine ${\gamma}-synthase$) and MetY (O-acetylhomoserine sulfhydrylase) from C. glutamicum were purified to homogeneity and the biochemical parameters were compared to assess the functional and evolutionary importance of each pathway. The molecular masses of the native MetB and MetY proteins were measured to be approximately 170 and 280 kDa, respectively, showing that MetB was a homotetramer of 40-kDa subunits and MetY was a homohexamer of 45-kDa subunits. The $K_m$ values for the O-acetylhomoserine catalysis effected by MetB and MetY were 3.9 and 6.4 mM, and the maximum catalysis rates were $7.4\;(k_{cat}=21\;S^{-1})\;and\;6.0\;(k_{cat}=28\;S^{-1})\;{\mu}mol\;mg^{-1}\;min^{-1}$, respectively. This suggests that both MetB and MetY can be comparably active in vivo. Nevertheless, the $K_m$ value for sulfide ions by MetY was 8.6mM, which was too high, considering the physiological condition. Moreover, MetB was active at a broad range of temperatures $(30\;and\;65^{\circ}C)$ and pH (6.5 and 10.0), as compared with MetY, which was active in a range from 30 to $45^{\circ}C$ and at pH values from 7.0 to 8.5. In addition, MetY was inhibited by methionine, but MetB was not. These biochemical data may provide insight on the role of the parallel pathways of methionine biosynthesis in C. glutamicum with regard to cell physiology and evolution.

BOUNDS ON PROBABILITY FOR THE OCCURRENCE OF EXACTLY r, t OUT OF m, n EVENTS

  • Lee, Min-Young
    • Communications of the Korean Mathematical Society
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    • v.12 no.2
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    • pp.393-401
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    • 1997
  • Let $A_1,A_2,\cdots,A_m$ and $B_1,B_2,\cdots,B_n$ be two sequences of events on a given probability space. Let $X_m$ and $Y_n$, respectively, be the number of those $A_i$ and $B_j$, which occur we establish new upper and lower bounds on the probability $P(X=r, Y=t)$ which improve upper bounds and classical lower bounds in terms of the bivariate binomial moment $S_{r,t},S_{r+1,t},S_{r,t+1}$ and $S_{r+1,t+1}$.

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Design and Implementation of Automatic Dependent Surveillance - Broadcast Server System (ADS-B 서버 시스템의 설계 및 구현)

  • Cho, Taehwan;Kim, Kanghee;Kim, Jinhyuk;Choi, Sangbang
    • Journal of Advanced Navigation Technology
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    • v.19 no.3
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    • pp.182-191
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    • 2015
  • The automatic dependent surveillance - broadcast (ADS-B) system which is a core system of aviation system block upgrade is designed and implemented. The ADS-B system consists of the 4 blocks and 10 units, and filtering algorithm is applied to the implemented ADS-B system. To evaluate a performance of the implemented ADS-B system, real aircraft position data is used and compared reliable radar data. The comparison results show that average position difference of 99.57 m. In addition, comparisons of aircraft position data between the implemented ADS-B system employing filtering algorithm and the conventional ADS-B system under various situations are carried out, such as aircraft turning, taking off, landing, and cruising. The comparison results show that average position difference of 8.02 m.

Effects of particle size and oxygen contents on ZrB2 powder for densification (ZrB2 분말의 입도 및 산화도가 치밀화에 미치는 영향)

  • Jung, Se-Hyuk;Choi, Sung-Churl
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.22 no.5
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    • pp.247-253
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    • 2012
  • In this study, two pretreatment methods were used to improve the sinterability of zirconium diboride ($ZrB_2$). As a mechanical treatment, as-received $ZrB_2$ powder was crushed using SPEX mill from an average size of $2.61{\mu}m$ to $0.35{\mu}m$. As a chemical treatment, oxygen contents of $ZrB_2$ powder were decreased from 4.20 wt% to 2.22 wt% using a dilute hydrofluoric solution. The relative density of sintered $ZrB_2$ increased with decreasing particle size and oxygen contents. But it is considered that particle size is more effective than oxygen contents for $ZrB_2$ densification. Through the two pretreatment processes, we produced sintered $ZrB_2$ ceramic with a full density without sintering additives. The sinterability of $ZrB_2$ was improved by using mechanical and chemical pretreatment methods.

A study on the fabrication of Y-branch for optical power distribution and its coupling properties with optical fiber (광분배를 위한 Y-branch 제작과 광파이버와의 결합특성에 관한 연구)

  • 김상덕;박수봉;윤중현;이재규;김종빈
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.21 no.12
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    • pp.3277-3285
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    • 1996
  • In this paper, w designed an opical power distribution device for application to an optical switching and an optical subscriber loop. We fabricated PSG thin film by LPCVD. Based on the measured index of fabricted thin film, rib-type waveguide was transformed to two-dimension by the effective index method and we simulated dispersion property to find asingle-mode condition. We found that the optimum design parameters of rib-type waveguide are:cladding layer of 3.mu.m, core layer of 3.mu.m, buffer layer of 10.mu.m, and core width of 4.mu.m. Each side of the guiding region was etched down to 4.mu.m to shape the core. We used these optimum parameters of the rib-type waveguide with branching angle of 0.5.deg. and simulted the Y-branch waveguide by the BPM simulation. Numerical loss in branching area was claculated to be 0.1581dB and equal to the total loss of the Y-branch. The loss of the fabricated Y-branch waveguide on PSG film ws 1.6dB at .lambda.=1.3.mu.m before annealing but was 1.2dB after annealing at 1000.deg. C for 10 minutes. Consequently, the loss of branching area from 3000.mu.m to 6000.mu.m in the z-direction was 0.8dB, and single-mode propagation was confirmed by measuring the near field pattern. For coupling the fabricated Y-branch waveguide with an optical fiber, we fabricated V-groove which was used as the upholder of optical fiber. An etching angle was 54.deg. and the width and depth of guiding groove was 150.mu.m, 70.mu.m, respectively. The optical fiber is inserted onto V-groove. Both the Y-branch and V-groove were connected through the index matching oil. Coupling loss after connecting Y-branch and the optical fiber on V-groove was 0.34dB and that after injecting index mateching oil was 0.14dB.

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Molecular Systematics of the Genus Megoura (Hemiptera: Aphididae) Using Mitochondrial and Nuclear DNA Sequences

  • Kim, Hyojoong;Lee, Seunghwan
    • Molecules and Cells
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    • v.25 no.4
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    • pp.510-522
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    • 2008
  • To construct the molecular systematics of the genus Megoura (Hemiptera: Aphididae), DNA based-identification was performed using four mitochondrial and three nuclear DNA regions: partial cytochrome c oxidase I (COI), partial tRNA-leucine + cytochrome c oxidase II (tRNA/COII), cytochrome b (CytB), partial 12S rRNA + tRNA-valine + 16S rRNA (12S/16S), elongation factor-1 alpha ($EF1{\alpha}$), and the internal transcribed spacers 1 and 2 (ITS1, ITS2). Pairwise sequence divergences between taxa were compared, and phylogenetic analyses were performed based on each DNA region separately, and the combined datasets. COI, CytB, $EF1{\alpha}$, ITS1, and ITS2 were relatively effective in determining species and resolving their relationships. By contrast, the sequences of tRNA/COII and 12S/16S were not able to separate the closely related species. CytB and $EF1{\alpha}$ gave better resolution with higher average sequence divergences (4.7% for CytB, 5.2% for $EF1{\alpha}$). The sequence divergence of COI (3.0%) was moderate, and those of the two ITS regions (1.8% for ITS1, 2.0% for ITS2) were very low. Phylogenetic trees were constructed by minimum evolution, maximum parsimony, maximum likelihood, and Bayesian phylogenetic analyses. The results indicated that the phylogenetic relationships between Megoura species were associated with their host preferences. Megoura brevipilosa and M. lespedezae living on Lespedeza were closely related, and M. nigra, monophagous on Vicia venosa, was rather different from M. crassicauda, M. litoralis, and M. viciae, which are oligophagous on Lathyrus and Vicia. The three populations of M. crassicauda formed a clade separated from M. litoralis and M. viciae. Nevertheless M. litoralis and M. viciae, which are morphologically similar, were not separated due to negligible sequence divergence. We discuss the phylogenetic relationships of the Megoura, and the usefulness of the seven DNA regions for determining the species level phylogeny of aphids.

Magnetic Propwrties of High Quality $Nd_{12}Fe_{80}B_{6}(Nb, M)$ (M=Ti/Cu/Ga) Melt-Spun Ribbons (고특성 $Nd_{12}Fe_{80}B_{6}(Nb, M)$ (M=Ti/Cu/Ga)급속응고리본의 자기특성)

  • 김윤배;김창석;김동환;이갑호;김택기
    • Journal of the Korean Magnetics Society
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    • v.2 no.1
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    • pp.44-49
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    • 1992
  • Magnetic properties and microstructures of $Nd_{12}Fe_{80}B_{6}(Nb,\;M)$ (M=Ti/Cu/Ga) melt-spun ribbons prepared by single wheel technique have been studied. The results of microstructural study have shown that Ga is effective for the orientation of c-axis normal to the ribbon plane. The Ga-added melt-spun ribbon, $Nd_{12}Fe_{80}B_{6}(Nb,\;Ga)$, quenched at $V_{s}=17.9\;m/s$ was mostly composed of fine grains of about 30 nm in size with the textured free-side surface. The powder of this ribbon aligned in mag-netic field showd a high remanence of 0.87 T which was about 5 % higher than that of ribbon itself. It is believed that there is a possibility to fabricate a new type of HIREM melt-spun ribbon with highly textured free-side surface.

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Microwave Dielectric Properties of $(Pb_{1-x}Ca_x)ZrO_3$ and $(Pb_{0.63},Ca_{0.37-x}M_x)ZrO_3$ (M = Mg, Sr) Ceramics ($(Pb_{1-x}Ca_x)ZrO_3$$(Pb_{0.63},Ca_{0.37-x}M_x)ZrO_3$ 세라믹스의 고주파 유전 특성)

  • 윤중락;이헌용
    • Electrical & Electronic Materials
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    • v.10 no.6
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    • pp.533-540
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    • 1997
  • The microwave dielectric properties of ((P $b_{1-x}$ C $a_{x}$)Zr $O_3$ and (P $b_{0.63}$,C $a_{0.37-x}$ $M_{x}$)Zr $O_3$(M=Mg,Sr) ceramics were investigated. In (P $b_{1-x}$ C $a_{x}$)Zr $O_3$ (X=0.33~0.40) ceramics, high quality factor and small temperature coefficient of resonant frequency were obtain in (P $b_{0.63}$C $a_{0.37}$)Zr $O_3$with perovskite structure. In the case of (P $b_{0.63}$C $a_{0.37-x}$M $g_{x}$)Zr $O_3$ dielectric constant temperature coefficient of resonant frequency increased and quality factor decreased due to increase of polarization of A-O bonding. When replacing Ca ion with Sr ion with large ion radius, polarization decreased with increased of bonding length and thus dielectric constant and temperature coefficient of resonant frequency decreased.decreased.creased.

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