• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1481-1489
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• 2016

The emergence and dissemination of carbapenemase-producing Acinetobacter baumannii isolates have been reported worldwide, and A. baumannii isolates harboring bla_OXA-23 are often resistant to various antimicrobial agents. Antimicrobial resistance can be particularly strong for biofilm-forming A. baumannii isolates. We investigated the genetic basis for carbapenem resistance and biofilm-forming ability of multidrug-resistant (MDR) clinical isolates. Ninety-two MDR A. baumannii isolates were collected from one university hospital located in the Chungcheong area of Korea over a 5-year period. Multiplex PCR and DNA sequencing were performed to characterize carbapenemase and bap genes. Clonal characteristics were analyzed using REP-PCR. In addition, imaging and quantification of biofilms were performed using a crystal violet assay. All 92 MDR A. baumannii isolates involved in our study contained the bla_OXA-23 and bap genes. The average absorbance of biomass in Bap-producing strains was much greater than that in non-Bap-producing strains. In our study, only three REP-PCR types were found, and the isolates showing type A or type B were found more than 60 times among unique patients during the 5 years of surveillance. These results suggest that the isolates have persisted and colonized for 5 years, and biofilm formation ability has been responsible for their persistence and colonization.

• Parasites, Hosts and Diseases
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• 제43권4호
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• pp.149-156
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• 2005

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

• 한국CDE학회논문집
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• 제1권1호
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• pp.20-32
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• 1996

The boundary evaluation technique for Boolean operation on non-manifold models which is regarded as the most popular and powerful method to create and modify 3-D CAD models has been developed. This technique adopted the concept of Merge and Selection in which the CSG tree for Boolean operation can be edited quickly and easily. In this method, the merged set which contains complete information about primitive models involved is created by merging primitives one by one, then the alive entities are selected following the given CSG tree. This technique can support the hybrid representation of B-rep(Boundary Representation) and CSG(Constructive Solid Geometry) tree in a unified non-manifold model data structure, and expected to be used as a basic method for many modeling problems such as data representation of form features, and the interference between them, and data representation of conceptual models in design process, etc.

• Korea-Australia Rheology Journal
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• 제18권2호
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• pp.41-49
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• 2006

A modified Volume-of-Fluid (VOF) numerical method is used to predict the dynamics of a liquid drop of a low viscosity dilute polymer solution, forming in air from a circular nozzle. Viscoelastic effects are rep-resented using an Oldroyd-B model. Predicted drop shapes are compared with experimental observations. The main features, including the timing of the shape evolution and the 'bead-on-a-string' effect, are well reproduced by the simulations. The results confirm published conclusions of the third author, that the deformation is effectively Newtonian until near the time of Newtonian pinch-off and that the elastic stress becomes large in the pinch region due to the higher extensional flow there.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1993년도 추계학술대회 논문집
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• pp.675-680
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• 1993

컴퓨터를 이용한 제품의 설계시 개념설계부터 최종설계에 이르는 동안 설계모델은 많은 수정을 요하게 된다. 이 과정에서 개념설계 단계부터 솔리드 모델을 채용하는 것은 불편하므로, 와이어프레임 모델이나 곡면 모델을 이용하여 설계를 진행하다가, 최종설계 단계에서 솔리드 모델로 전환하는 것이 바람직하다. 이 경우 이 3가지 모델을 모두 지원하는 모델러가 요구되는데 '복합다양체'를 지원하는 모델러가 이 요건을 만족시킨다. 또한 경계표현(B-rep)방식으로 모델링시 불리안 작업자를 많이 이용하는데, 모델링 도중에 불리한 작업으로 생성된 모델의 Undo작업은 용이하지 않은 일이다. 따라서 불리안 작업으로 생성된 모델의 수정작업을 위한 알고리즘이 요구된다, 일한 수정작업을 위해선 복합다양체를 지원하는 자료구조가 필요하다. 본 논문에선 이러한 복합다양체 자료구조를 갖는 형상모델러의 기본적 자료구조와 기본물체 모델링시 오일러 작업자를 구현하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.833-835
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• 2002

In this paper, we propose multi-resolutional representation of B-rep solid models using the selective Boolean operations on non-manifold geometric models. Since the union and subtraction operations of the selective Boolean operations are commutative, the integrity of the model is guaranteed for reordering design features. A multi-resolution representation is established using a non-manifold merged set model and a feature modeling tree reordered according to some criterion of level of detail (LOD). Then, a solid model for a specified LOD can be extracted from this multi-resolution model using the selective Boolean operations.

• 대한기계학회논문집
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• 제17권10호
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• pp.2446-2456
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• 1993

By virtue of progress of computer science, CAD/CAM technology has been developed greatly in each area. But the problems in the integration of CAD/CAM are not yet solved completely. The reason is that the exchange of data between CAD and CAM is difficult because the domains of design and manufacturing are different in nature. To solve this problem, a feature based modeller is developed in this study, which makes it possible to communicate between design and manufacturing through features. The modeller has feature, the concept of semi-bounded plane is introduced, and implemented as a B-rep sheet model using half-edge data structure. The features are then created on a part by local modification of the boundary on a part based on feature template information. This approach generalizes the modelling of features in a geometry model.

• 한국CDE학회논문집
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• 제2권3호
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• pp.150-160
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• 1997

Existing non-manifold data structures which use the ordered topological representation method, are designed based on a "Model" which is the highest topological entity. Their non-manifold information is always included in edges and vertices even if they are in the manifold situation. Thus they require large storage spaces than manifold data structures. The proposed data structure reduces its storage space by removing unnecessary information stored in edges and vertices. Topological information is classified into manifold and non-manifold information. The main non-manifold information is radial cycles and disk cycles. The proposed data structure always stores manifold information. For the non-manifold situation, the edge stores radial cycles, and the vertex stores disk cycles. The storage space can be reduced in the later stage of CAD design when the ratio of non-manifold to manifold entities is small.

• 대한기계학회논문집A
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• 제30권5호
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• pp.488-495
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• 2006

The demand for the use of 3D CAD data over the Internet environment has been increased. However, transmission of 3D CAD data has delayed the communication effectiveness because of the CAD data size. Lightweight CAD file design methodology is required for rapid transmission in the distributed environment. In this paper, to derive lightweight CAD files from commercial CAD systems, a file translation system producing a native file is constructed first by using the InterOp and API of the ACIS kernel. Using the B-rep model and mesh data extracted from the native file, the lightweight CAD file with topological information is constructed as a binary file. Since the lightweight CAD file retains topological information, it is applied to the dimensional verification, digital mock-ups and visualization of CAD files. Effectiveness of the proposed lightweight CAD file is confirmed through various case studies.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1997년도 추계학술대회 논문집
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• pp.105-108
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• 1997

To reduce design period, demands for rapid prototyping system is increasing. Among the rapid prototyping system, as a concept modeler, 3D Printer appears before the footlights. In spite of short production time and convenience, 3D Printer causes post processing problem. And that is too slow to adapt to the demand of workshop. In this research, we developed a system for reducing build time and roughness using splitting operation. Also, propose BFS(B-rep For STL) data structure to maintain model data, split STL model and implement Euler Operation for input and output.