Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Expressed sequence tags analysis of Blattella germanica

  • CHUNG Hyang Suk (Department of Otorhinolaryngology, Kosin University College of Medicine) ;
  • YU Tai Hyun (Department of Otorhinolaryngology, Kosin University College of Medicine) ;
  • KIM Bong Jin (Department of Parasitology, Kosin University College of Medicine) ;
  • KIM Sun Mi (Department of Parasitology, Kosin University College of Medicine) ;
  • KIM Joo Yeong (Department of Parasitology, Kosin University College of Medicine) ;
  • YU Hak Sun (Department of Parasitology, Pusan National University College of Medicine) ;
  • Jeong Hae Jin (Department of Parasitology, Pusan National University College of Medicine) ;
  • OCK Mee Sun (Department of Parasitology, Kosin University College of Medicine)
  • Published : 2005.12.01
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Abstract

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

Keywords

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