• 한국CDE학회논문집
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• 제1권2호
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• pp.122-132
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• 1996

Features are generic shapes with which engineers associate certain attributes and knowledge useful in reasoning about the product. Feature-based modeling systems support additional levels of information beyond those available in geometric modelers. The objective of this study is to develop a PC level feature-based modeling system which explicitly represents dimensions of the part. The feature-based modeler retains all the benefits of traditional B-rep. solid models, and represents the dimensions at a high level of a abstraction so that dimension driven geometry can be achieved.

• 한국정밀공학회지
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• 제10권1호
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• pp.89-99
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• 1993

A solid modeller was developed in this paper. Representation scheme of modelling is nonmanifold B-rep. While namy B-rep modellers use edges as basic element of modelling, this modeller is triangle-based. New modelling element, split triangle that is composed of 3 pure half-edges and has no information about the adjoining triangles, was defined to enlarge the range of representation. Corresponding algorithms for Boolean set operations were also developed.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.482-488
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• 2019

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. Replicase (Rep) proteins are considered essential for viral replication. Capsid (Cap) protein is the primary immunogenic protein that induces protective immunity. Little is known about comparison on the immunogenicity of PCV2 Rep and Cap fusion protein and Cap protein. In the present study, recombinant baculoviruses expressing the Rep-Cap fusion protein (Bac-Rep-Cap) and the Cap protein (Bac-Cap) of PCV2 were constructed and confirmed with western blot and indirect fluorescence assay. Immunogenicities of the two recombinant proteins were tested in mice. The titers of antibodies were determined with a PCV2-specific enzyme-linked immunosorbent assay (ELISA) and a serum neutralization assay. The $IFN-{\gamma}$ response of immunized mice was measured by ELISA. The mice immunized with the Bac-Rep-Cap and Bac-Cap successfully produced Cap-specific immunoreaction. The mice immunized with the Bac-Cap developed higher PCV2-specific neutralizing antibody titers than mice injected with the Bac-Rep-Cap. $IFN-{\gamma}$ in the Bac-Rep-Cap group was increased compared to those in the Bac-Cap group. Vaccination of mice with the Bac-Rep-Cap showed significantly decreased protective efficacy compared to the Bac-Cap. Our findings will indubitably not only lead to a better understanding of the immunogenicity of PCV2, but also improved vaccines.

• Spatial Information Research
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• 제19권1호
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• pp.93-105
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• 2011

최근 들어 건축과 IT 기술이 발전함에 따라서 대규모의 복잡한 3차윈 공간이 증가하고 있고, 사람들 또한 이러한 공간에서 생활하는 시간이 늘어나고 있는 추세이다. 이에 따라 복잡한 3차원 공간 내에서 유사시를 대비한 또는 편의 정보를 제공하기 위한 서비스가 필요하게 되었고, 이러한 서비스를 제공하기 위해서는 복잡한 공간에서의 효율적인 위상학적 관계성 파악이 기본적으로 뒷받침이 되어야 한다. 공간의 관계성 파악은 위상학적 관계를 저장하고 표현하는 각각의 데이터 모델에 따라 그 방법과 효율성이 달라진다. 위상학적 관계성 저장과 표현에 있어서 가장 널리 사용되어 왔던 데이터 모델은 B-rep 기반의 데이터 모델이고, 2000년대 초반에 들어서는 네트워크 기반 위상학적 데이터 모델에 관한 연구가 많이 진행되었다. 본 연구에서는 데이터 저장 용량 및 공간질의에 대하여 두 데이터 모델의 효율성을 검증한다. 효율성은 데이터의 저장용량, 인접성 및 연결성 질의 응답속도를 기준으로 하여 수행한다. 효율성 검증의 결과로써 네트워크 기반 위상학적 데이터 모델이 3차원 공간질의에 있어서 B-rep 기반 데이터 모델 보다 효율성이 높음을 제시한다.

• 한국CDE학회논문집
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• 제11권4호
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• pp.289-302
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• 2006

It is becoming a common trend that many designers work on a very complex assembly together in a collaborative environment. In this environment, every designer should be able to see the whole assembly in a full detail or in a rough shape at least. Even though the hardware technology is being improved very rapidly, it is very difficult to display a very complex assembly at a speed to allow smooth interactions for designers. This problem could be solved if a designer could manipulate his portion of the assembly in a full resolution while the remaining portion of the assembly is displayed in a rough resolution. It is also desired that the remaining portion is converted to the full resolution when needed. To realize this environment, the capabilities to simplify the portions of an assembly and to reset to the original resolution should be added to the current CAD systems. Thus operators realizing multi-resolution on B-rep are proposed in this paper. They are: wrap-around, smooth-out, and thinning operator. Through appropriately applying these operators sequentially, an assembly model of any desired resolution can be easily generated. Of course, the assembly can go back to the finer resolution. In this paper, the data structures and the processes to realize these operators are described and a prototype modeling system with these operators is also demonstrated.

• 한국CDE학회논문집
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• 제6권1호
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• pp.48-58
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• 2001

One of the biggest problems of rapid prototyping(RP) technologies lies in their standard file format for CAD data exchange. Current methods using the de facto industry standard 'STL'have at times resulted in problems such as accuracy, redundancy, and integrity. In this paper we propose a STEP based data exchange framework for rapid prototyping systems. In this paradigm of data exchange, STEP models can be imported and converted into faceted B-rep. solid models for visualization and 2-D layer data for RP. Also an STL model, on the other hand, can be converted into a faceted B-rep. STEP model and exported as a new data exchange model with RP information.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.836-839
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• 2002

This paper describes the selective Boolean operations on non-manifold geometric models whose union and subtraction operations are communicative. These operations guarantee the same resulting shape in spite of change of the order of Boolean operations, and the integrity of the model for omission of some features. In addition, a B-rep model for a modified modeling history is obtained in a short time, as no boundary evaluation is necessary. These features enable easy implementation of multi-resolution representation of B-rep models.

• Journal of Mechanical Science and Technology
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• 제19권2호
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• pp.558-566
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• 2005

We propose a feature-based multi-resolution representation of B-rep solid models using history-based Boolean operations based on the merge-and-select algorithm. Because union and subtraction are commutative in the history-based Boolean operations, the integrity of the models at various levels of detail (LOD) is guaranteed for the reordered features regardless of whether the features are subtractive or additive. The multi-resolution solid representation proposed in this paper includes a non-manifold topological merged-set model of all feature primitives as well as a feature-modeling tree reordered consistently with a given LOD criterion. As a result, a B-rep solid model for a given LOD can be provided quickly, because the boundary of the model is evaluated without any geometric calculation and extracted from the merged set by selecting the entities contributing to the LOD model shape.

• 대한임상검사과학회지
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• 제36권1호
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• pp.1-6
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• 2004

The enterobacterial repetitive intergenic consensus (ERIC)-PCR is a recently described DNA fingerprinting technique based on amplification of repetitive element distributed in bacteria. We applied of ERIC-PCR to clinical isolates of Vibrio parahaemolyticus and other bacteria associated diarrhea. Twenty isolates of V. parahaemolyticus were used for intragenic genotyping, which were isolated from 2001 to 2002 in Chungbuk National University hospital. For interspecies genotyping, V. vulnificus, V. alginolyticus, V. parahaemolyticus, Escherichia coli, Salmonella and Shigella spp. were used. The genotyping were analyzed by ERIC-PCR. The genotyping of V. parahaemolyticus were grouped two major pattern (A, B) and were subdivided into 10 subtypes (A1, A2, B1-B8) by ERIC-PCR. These method distinctly differentiated bacterial species associated diarrhea. Those results show that ERIC-PCR can be reliable and efficient method for genotyping of V. parahaemolyticus and bacteria associated diarrhea.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.135.2-136
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• 2003

To characterize plasmids in Xanthomonu axonopodis pv. glycines, we isolated plasmids pAG1 from the strain AG1 and pXAG81 and PXAG82 from the strain Bra, respectively, and sequenced three plasmids. The size of plasmids, pAG1, pXAG81, and pXAG82 was 15,149-base pairs (bp), 26,727-bp, and 1,496-bp, respectively Fifteen and twenty six possible open reading frames (ORFs) were present in pAG1 and pXAG81, respectively. Only one ORF homologous to a rep gene of Xylella fastidiosa was present in pXAG82. pAG1 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, three parA genes, M.XmaI, R.XmaI, and six hypothetical proteins. pXAG81 contained genes homologous to avrBs3, tnpA, tnpR, repA, htrA, two parA genes, pemI, pemK, mobA, mobB, mobC, mobD, mobE, trwB, traF, traH, ISxac2, and eleven hypothetical proteins. Based on DNA sequence analysis, we presume that pXAG81 is a conjugal plasmid. Interestingly, we found 0.5-kb truncated avirulence gene similar to aurXacE3 on the right border of avrBs3 homolgs of pAG1 and pXAG81. Two hundred twenty five isolates were analyzed to find aurBS3 or tra gene homologs by Southern hybridization. The numbers of avrBs3 homolog varied from 3 in AG1 to 8 in AG166. Two hundred seventeen isolates appeared to can conjugative plasmids (pXAG81 type), and thirty eight isolates appeared to carry non-conjugative plamids (pAGl type). This indicated that aurBs3 gene homologs might be spread by conjugation in X. axonopodis pv. glycines.