대한임상검사과학회지 (Korean Journal of Clinical Laboratory Science)
- 제36권1호
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- Pages.1-6
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- 2004
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- 1738-3544(pISSN)
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- 2288-1662(eISSN)
Repetitive Element-PCR (rep-PCR)을 이용한 Vibrio parahaemolyticus 의 분자유전학적 아형 분류
Molecular Typing of Vibrio parahaemolyticus by Repetitive Element-PCR (rep-PCR)
- 김원식 (충북대학교병원 진단검사의학과) ;
- 홍승복 (분당제생병원 진단검사의학과) ;
- 이경 (서울대학교 의과대학 분당병원 진단검사의학과) ;
- 이정남 (단국대학교 첨단과학부 미생물학과) ;
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신경섭
(충북대학교 의과대학 진단검사의학과 교실)
- Kim, Won Sik (Dept. of Laboratory Medicine, Chungbuk National University Hospital) ;
- Hong, Seung Bok (Dept. of Laboratory Medicine, Pundang Jesaeng General Hospital) ;
- Lee, Kyung (Dept. of Laboratory Medicine, Seoul National University Bundang Hospital) ;
- Lee, Jung Nam (Dept. of Microbiology, Dankook University) ;
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Shin, Kyeong Seob
(Dept. of Laboratory Medicine, Chungbuk National University College of Medicine)
- 발행 : 2004.06.30
초록
The enterobacterial repetitive intergenic consensus (ERIC)-PCR is a recently described DNA fingerprinting technique based on amplification of repetitive element distributed in bacteria. We applied of ERIC-PCR to clinical isolates of Vibrio parahaemolyticus and other bacteria associated diarrhea. Twenty isolates of V. parahaemolyticus were used for intragenic genotyping, which were isolated from 2001 to 2002 in Chungbuk National University hospital. For interspecies genotyping, V. vulnificus, V. alginolyticus, V. parahaemolyticus, Escherichia coli, Salmonella and Shigella spp. were used. The genotyping were analyzed by ERIC-PCR. The genotyping of V. parahaemolyticus were grouped two major pattern (A, B) and were subdivided into 10 subtypes (A1, A2, B1-B8) by ERIC-PCR. These method distinctly differentiated bacterial species associated diarrhea. Those results show that ERIC-PCR can be reliable and efficient method for genotyping of V. parahaemolyticus and bacteria associated diarrhea.