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Developmental Potentials of Clone Embryos Derived from Bovine Fetal Fibroblast Cells (소 태아섬유아세포 유래 복제란의 발육능에 관한 연구)

  • Cheong, H.T.;Kwon, D.J.;Park, Y.S.;Hwang, H.S.;Park, C.K.;Yang, B.K.;Kim, C.I.
    • Korean Journal of Animal Reproduction
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    • v.24 no.1
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    • pp.49-57
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    • 2000
  • This study was conducted to investigate the developmental potential of cloned embryos derived from bovine fetal fibroblast cells, and the effect of quiescent treatment, passage number and origin of donor cells on in vitro development of cloned embryos. Fetal skin and liver-derived fibroblast cells were transferred to enucleated oocytes after serum starvation or nontreatment (cycling). After electrofusion. reconstituted embryos were activated with $Ca^{++}$-ionophore and cycloheximide, and cocultured for 7~9 days with BRL cells. Some blastocysts were transferred to recipient cows 7~8 days post estrus. The development rate to the blastocyst stage of serum starved cell-derived embryos was higher (25.3%) than that of actively dividing cells-derived embryos (15.9%), The rates of blastocyst formation were 23.1~25.0% after transfer of cell passaged 4 to 6 times, and 23.8 and 25.2% after transfer of fetal skin and liver cells, respectively. After embryo transfer, 34.4% and 15.6% of recipient cows were pregnant on Day 60 and 120, respectively, and one male calf was produced from skin-derived vitrified blastocyst. The result of this study showed that the development of cloned embryos. was enhanced by quiescent treatment, but did not different among the cells passaged 4 to 6 times, and between skin and liver cells. This result also confirms that offspring can be obtained from the vitrified clone embryo derived from fetal skin cell.

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A Study on "Reason and Madness" in Hegel's 『Phenomenology of Spirit』 - An Interpretation searching for the possibility of the dialogue between Hegel and Lacan - (헤겔 『정신현상학』에서의 '이성과 광기'의 문제 - 헤겔의 라캉과의 대화 가능성에서 본 하나의 해석 -)

  • Lee, Jong-chul
    • Journal of Korean Philosophical Society
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    • v.115
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    • pp.249-279
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    • 2010
  • The Law of the Heart, which appears in the chapter "B. The Actualization of Rational Self-consciousness Through its own Activity" in Hegel's Phenomenology of Spirit represents that the self-certainty of the reason may be the another face of the Madness. The Reason's indubitable certainty is the testimony of the truth for Descartes, and it also plays a role as the moral maxim of the conscience for Kant. But this subjective certainty unavoidably leads to the Madness of self-conceit, which unifies the consciousness and the reality ignoring the difference between them. The typical attitude appearing in the reformer like Don Quixote and also romantic idealists reveals the fact that modern reason and the psychosis can be both faces of one and the same coin. The Mirror Stage, the Imaginary, and the Formula of the Desire in Lacan's Theory shows that the Image of completeness is the result of Mis-understanding. Even if the Mirror stage is necessary for the Formation of the subject, at the same time it should also lead to the next stage, i.e., the Symbolic Order. It is considered as the realm of the Otherness, which refers to the realm of language or that of law. If the Ego can't go through the symbolic castration acted on by the Name of the Father, he will be remained in the prison of the Imaginary. The Madness also shows the similar process. For Hegel the Discipline of the Labor or the Death as absolute Otherness that inevitably delays the Desire plays the same role as the name of Father. It may be the experience of Separation or that of Sublation of the individual towards the universal, which is equivalent to the experience of Symbolic castration in Lacan. Furthermore there may be the difference between Hegel and Lacan as the following; while in Hegel the experience of Separation is founded on the spontaneity of the Spirit, for Lacan it is to be compelled and structured by the absolute Other.

Physico-chemical Meat quality and sensory properties of Holstein steer beef by different feeding conditions of agricultural by-product TMR (농산부산물 TMR 사료의 에너지 급여조건에 따른 홀스타인 육우육의 이화학적 육질 및 관능특성)

  • Cho, Soohyun;Seong, Pilnam;Kang, Sunmoon;Kang, Geunho;Kim, Youngchun;Choi, Sun Ho
    • Journal of agriculture & life science
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    • v.50 no.1
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    • pp.211-222
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    • 2016
  • This study was conducted to investigate the physico-chemical meat quality and sensory properties of Holstein steers(n=18, 4 months old, average body weight 160kg) which were divided into 3 groups and fed 2 different energy levels of total mixed rations(TMR)(T1, TDN 74~84%; T2, TDN 77~85%) until 18, 20 or 22 months old, respectively. For loin muscles, 22-month group of T1 and 20- and 22-month groups of T2 had higher fat contents than the other groups (P<0.05). T2 had higher WHC values for 18-month groups among 3 feeding groups (P>0.05). In sensory properties, T1 had higher tenderness, flavor-likeness and overall likeness for 20-month group than the other groups (P<0.05). T2 had higher tenderness, juiciness, flavor likeness and overall likeness in 20- and 22-months groups than 18-month group (P<0.05). For top round muscles, 22-month group had higher fat contents than the other groups of T1 and T2 (P<0.05). T1 had higher in redness(a* value) and yellowness (b* value) of 22-month group (P<0.05). T2 had higher WHC values, lower WBS and CL values in 18-month group than the other groups (P<0.05). In sensory properties, T1 had higher tenderness for 18 and 20-month groups and overall likeness (P<0.05). T2 had higher tenderness in 18-month group. The results of this study showed that longer feeding of agricultural by-product TMR increased the intramuscular fat contents, however, the 18 and 20-months finishing groups were advantageous for the meat quality and sensory properties of Holstein steers.

Study on Nozzle Type and Proper Discharge Pressure of Sprayer for Vehicle Disinfecting System (차량소독장치용 노즐형태와 분무기의 적정토출압력에 관한 연구)

  • Lim, Young-Il;Chang, Dong-Il;Kim, Jeong-Chul;Park, Dong-Suk;Lee, Seung-Joo;Kang, Beom-Sun;Kim, Suk;Gutierrez, Winson M.;Lee, Tae-Hoon;Choi, Chung-Heon;Chang, Hong-Hee
    • Journal of agriculture & life science
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    • v.50 no.3
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    • pp.119-127
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    • 2016
  • The current disinfection method of vehicles being applied in South Korea has various shortcomings. So, the epidemic has generated continuously at livestock farms. It is very important to develop an effective disinfection system for reduction of the epidemic. And various basic data is required for this development. Therefore, this study was performed to identify the nozzle type and proper discharge pressure of sprayers. The experiment was conducted from January 10, 2012 until February 28, 2012. All the performance measurement experiments were repeated five times. The subjects of experiment were the A, B and C company's products. The sprayed capacity, angle of spray and the covering area ratio were measured for each product. As a result, the sprayed capacity, angle of spray and the covering area ratio were increased as the discharge pressure of the sprayer was increased. In conclusion, the conical shaped of the nozzle is considered more appropriate than V-shaped, and the proper discharge pressure is expected to be at least 20kg/㎠.

Estimation of Genetic Parameter for Milk Production and Linear Type Traits in Holstein Dairy Cattle in Korea (국내 Holstein 젖소의 유생산 형질과 유방 및 지제 선형심사 형질에 대한 유전모수 추정)

  • Won, J.I.;Dang, C.K.;Lim, H.J.;Jung, Y.S.;Im, S.K.;Yoon, H.B.
    • Journal of agriculture & life science
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    • v.50 no.1
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    • pp.167-178
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    • 2016
  • This study was conducted to estimate genetic parameters for milk production and linear type traits in Holstein dairy cattle in Korea. The data including milk yields, fat yields, protein yields, fat percent, protein percent, somatic score and 15 linear type traits for 10,218 first parity cows collected by Dairy Cattle Improvement Center, National Agricultural Cooperative, Korea, which were calving from January 2009 to April 2013. Genetic and error (co)variances between two traits selected form 19 traits were estimated using bi-trait pairwise analyses with WOMBAT package. The estimated heritabilities for milk yield(MY), fat yield(FY), protein yield(PY), fat percent(FP), protein percent(PP), somatic cell score(SCS), udder depth(UD), udder texture(UT), median suspensory(MS), fore udder attachment(FUA), front teat placement (FTP), rear attachment height(RAH), rear attachment width(RAW), rear teat placement(RTP), front teat length(FTL), foot angle(FA), heel depth(HD), bone quality(BQ), rear legs side view(RLSV), rear legs rear view(RLRV) and locomotion(LC) were 0.128, 0.144, 0.100, 0.273, 0.333, 0.090, 0.179, 0.066, 0.104, 0.109, 0.127, 0.099, 0.059, 0.069, 0.154, 0.014, 0.010, 0.052, 0.065, 0.175 and 0.031, respectively. Among the genetic correlations, UD, UT, FTP, RAW, FTL, FA and RLSV with MY were -0.334, 0.271, 0.445, 0.544, 0.076, -0.281 and -0.228, respectively, and MS, FTP, RTP, FTL, FA, BQ, RLSV, RLRV and LC with PP were -0.147, -0.182, -0.262, -0.136, 0.355, 0.311, 0.135, 0.233 and 0.143, respectively. Especially, MY had the highest positive genetic correlation with RAW (0.544), while SCS had the highest negative genetic correlation with LC (-0.603). FP had negative genetic correlation with most udder traits, whereas, FP had positive genetic correlation with leg and hoof traits (0.056 - 0.355).

The Effect of Participation Degree in Sports for all of People with Physical Disabilities on Positive Psychological Capital(PPC) (지체장애인의 생활체육 참여정도가 긍정심리자본(PPC)에 미치는 영향)

  • Kim, Dae-Kyung;Park, Jin-Woo;Kim, Hye-Min;Lee, Hyun-Su
    • 한국체육학회지인문사회과학편
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    • v.54 no.5
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    • pp.867-876
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    • 2015
  • This study was intended to closely examine an effect that the level of physically challenged person's participation in community sports had on positive psychological capital. In order to accomplish the purpose of study, data on 212 physically challenged persons who lived in B city and participated in community sports were analyzed. Korean version of positive psychological capital created by Taehong Lim (2014) through the reconstruction of scales developed by Luthans, Youssef and Avolio(2007) and Sangwan Jeon and Jonghun Yang's (2009) level of participation in community sports was reconstructed through modification·improvement as measurement instrument. An exploratory factor analysis, reliability test, paired difference test, and multiple regression analysis was carried out by using SPSS 18.0 program for data processing. First, It was shown that there was a significant difference in positive psychological capital according to gender, age, and disability grade among physically challenged persons' socio-demographic characteristics. Second, it was shown that, among sub-variables (period, frequency and intensity) of level of physically challenged persons' participation in community sports, the frequency of participation and the intensity of participation had a significant effect on self efficacy. On the other hand, it was shown that the period of participation didn't have a significant effect. Third, it was shown that the frequency of participation had a significant effect on optimism. On the other hand, it was shown that the period of participation and the intensity of participation didn't have a significant effect. Fourth, it was shown that the frequency of participation and the intensity of participation had a significant effect on hope. On the other hand, it was shown that no significant effect was produced on the period of participation. Fifth, it was shown that the frequency of participation had a significant effect on resilience. On the other hand, it was shown that no significant effect was produced on the period of participation and the intensity of participation. Sixth, it was shown that the frequency of participation and the intensity of participation had a significant effect on positive psychological capital. And it was shown that no significant effect was produced on the period of participation.

Present Status and Prospect of Valuation for Tangible Fixed Asset in South Korea (유형고정자산 가치평가 현황: 우리나라 사례를 중심으로)

  • Jin-Hyung Cho;Hyun-Seung O;Sae-Jae Lee
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.46 no.1
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    • pp.91-104
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    • 2023
  • The records system is believed to have started in Italy in the 14th century in line with trade developments in Europe. In 1491, Luca Pacioli, a mathematician, and an Italian Franciscan monk wrote the first book that described double-entry accounting processes. In many countries, including Korea, the government accounting standards used single-entry bookkeeping rather than double-entry bookkeeping that can be aggregated by account subject. The cash-based and single-entry bookkeeping used by the government in the past had limitations in providing clear information on financial status and establishing a performance-oriented financial management system. Accordingly, the National Accounting Act (promulgated in October 2007) stipulated the introduction of double-entry bookkeeping and accrual accounting systems in the government sector from January 1, 2009. Furthermore, the Korean government has also introduced International Financial Reporting Standards (IFRS), and the System of National Accounts (SNA). Since 2014, Korea owned five national accounts. In Korea, valuation began with the 1968 National Wealth Statistics Survey. The academic origins of the valuation of national wealth statistics which had been investigated by due diligence every 10 years since 1968 are based on the 'Engineering Valuation' of professor Marston in the Department of Industrial Engineering at Iowa State University in the 1930s. This field has spread to economics, etc. In economics, it became the basis of capital stock estimation for positive economics such as econometrics. The valuation by the National Wealth Statistics Survey contributed greatly to converting the book value of accounting data into vintage data. And in 2000 National Statistical Office collected actual disposal data for the 1-digit asset class and obtained the ASL(average service life) by Iowa curve. Then, with the data on fixed capital formation centered on the National B/S Team of the Bank of Korea, the national wealth statistics were prepared by the Permanent Inventory Method(PIM). The asset classification was also classified into 59 types, including 2 types of residential buildings, 4 types of non-residential buildings, 14 types of structures, 9 types of transportation equipment, 28 types of machinery, and 2 types of intangible fixed assets. Tables of useful lives of tangible fixed assets published by the Korea Appraisal Board in 1999 and 2013 were made by the Iowa curve method. In Korea, the Iowa curve method has been adopted as a method of ASL estimation. There are three types of the Iowa curve method. The retirement rate method of the three types is the best because it is based on the collection and compilation of the data of all properties in service during a period of recent years, both properties retired and that are still in service. We hope the retirement rate method instead of the individual unit method is used in the estimation of ASL. Recently Korean government's accounting system has been developed. When revenue expenditure and capital expenditure were mixed in the past single-entry bookkeeping we would like to suggest that BOK and National Statistical Office have accumulated knowledge of a rational difference between revenue expenditure and capital expenditure. In particular, it is important when it is estimated capital stock by PIM. Korea also needs an empirical study on economic depreciation like Hulten & Wykoff Catalog A of the US BEA.

Determining the Authenticity of Labeled Traceability Information by DNA Identity Test for Hanwoo Meats Distributed in Seoul, Korea (DNA 동일성 검사를 통한 서울지역 유통 한우육의 표시 이력정보 진위 판별)

  • Yeon-jae Bak;Mi-ae Park;Su-min Lee;Hyung-suk Park
    • Journal of Food Hygiene and Safety
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    • v.38 no.1
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    • pp.12-18
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    • 2023
  • Beef traceability systems help prevent the distribution of Hanwoo (Korean native cattle) meat as imported beef. In particular, assigning a traceability number to each cattle can provide all information regarding the purchased Hanwoo meat to the consumers. In the present study, a DNA identity test was conducted on 344 samples of Hanwoo meat from large livestock product stores in Seoul between 2021 and 2022 to determine the authenticity of important label information, such as the traceability number. Traceability number mismatch was confirmed in 45 cases (13.1%). The mismatch rate decreased to 11.3% in 2022 from 14.7% in 2021, and the mismatch rate was higher in the northern region (16.9%) than in the southern region (10.2%). In addition, of the six brands, B and D showed satisfactory traceability system management, whereas E and A showed poor traceability system management, with significant differences (P<0.001). The actual traceability number confirmation rate was only 53.9% among the mismatch samples. However, examination of the authenticity of label information of the samples within the identified range revealed false marking in the order of the traceability number (13.1%), sex (2.9%), slaughterhouse name (2.2%), and grade (1.6%); no false marking of breed (Hanwoo) was noted. To prevent the distribution of erroneously marked livestock products, the authenticity of label information must be determined promptly. Therefore, a legal basis must be established mandating the filling of a daily work sheet, including the traceability number of beef, in partial meat subdivisions. Our findings can be used as reference data to guide the management direction of traceability systems for ensuring transparency in the distribution of livestock products.

Eliminatory Effect of Health Drink Containing Hovenia Dulcis Thunb Extract on Ethanol-Induced Hangover in Rats (헛개나무 열매 추출물을 함유한 건강음료의 숙취 제거 효과)

  • Park, Eun-Mi;Ye, Eun-Ju;Kim, Soo-Jung;Choi, Hyun-Im;Bae, Man-Jong
    • Journal of the Korean Society of Food Culture
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    • v.21 no.1
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    • pp.71-75
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    • 2006
  • This study was conducted to investigate the eliminatory effect of health drink containing Hovenia dulcis Thunb extract on ethanol-induced hangover in rats. Male Sprague-Dawley rats weighing $200{\pm}10\;g$ were given health drink (10 mL/kg) or other company product(10 mL/kg) 30 min before or after 40% ethanol (5 g/kg body weight) ingestion. To study the effect of health drink on blood ethanol concentration, blood was taken from caudal artery at 1, 3, 5 hr and the animal were sacrificed 24 hr after ethanol ingestion. From 1 to 5 hr, health drink pre- or postdosing significantly decreased the ethanol levels in the blood. The acetaldehyde concentration showed decrement in health drink group and other company product group. The activities of ethanol, alcohol dehydrogenase and aldehyde dehydrogenase measured at postdosing, were also not altered by the administration of health drink. Alanine aminotransferase and aspartate aminotransferase activities showed unaltered resulted in all experimental groups compared with the normal group. These results suggest that oral intake of health drink containing Hovenia dulcis Thunb is effective on elimination of ethanol-induced hangover.

Quality Characteristics of Cuttlefish Inky Tofu Prepared with Various Coagulants (응고제에 따른 오징어 먹물 두부의 품질 특성)

  • Park, Eo-Jin;An, Sang-Hee
    • Journal of the Korean Society of Food Culture
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    • v.21 no.6
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    • pp.653-660
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    • 2006
  • Some quality characteristics of tofu prepared with cuttlefish ink were investigated to study the effects of various of coagulants. Each concentration of coagulant was determined as 0.2% of GDL, 0.3# of $MgCl_2$, 1%^ of $CaCl_2$, 1.5% of $CaSO_4$ and 0.6% D-gluconic acid calcium by pre-experiment. Also, the optimum concentration of added cuttlefish ink was chosen as 3%(diluted in twenty times). The yield of inky tofu prepared with GDL as coagulant was the highest. According to prepared with $MgCl_2$ was the highest. The result of microstructure was examined by SEM, the particles of inky tofu coagulated with GDL and D-gluconic acid calcium were small and uniformity. In overall acceptability of sensory properties, inky tofu coagulated with GDL was the highest in score. In the color of inky tofu, L value and a value were the highest coagulated with GDL, but that coagulated with $CaCl_2$ had the highest b value. In the texture properties of inky tofu, hardness, gumminess and brittleness were the highest coagulated with D-gluconic acid calcium. A positive correlation was observed between the pH of tofu whey and acidity. Sensory properties of roasted nutty flavor, hardness, cohesiveness and springiness were positively correlated with the acceptability.