Eliminatory Effect of Health Drink Containing Hovenia Dulcis Thunb Extract on Ethanol-Induced Hangover in Rats

헛개나무 열매 추출물을 함유한 건강음료의 숙취 제거 효과

  • Park, Eun-Mi (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Ye, Eun-Ju (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Kim, Soo-Jung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technopark) ;
  • Choi, Hyun-Im (Dept. of occupational therapy, Sungduk college) ;
  • Bae, Man-Jong (Dept. of Oriental Medicine Biofood Science, Daegu Haany University)
  • 박은미 ((재) 경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 예은주 ((재) 경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김수정 ((재) 경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 최현임 (성덕대학 작업치료과) ;
  • 배만종 (대구한의대학교 한방바이오식품과학과)
  • Published : 2006.02.28

Abstract

This study was conducted to investigate the eliminatory effect of health drink containing Hovenia dulcis Thunb extract on ethanol-induced hangover in rats. Male Sprague-Dawley rats weighing $200{\pm}10\;g$ were given health drink (10 mL/kg) or other company product(10 mL/kg) 30 min before or after 40% ethanol (5 g/kg body weight) ingestion. To study the effect of health drink on blood ethanol concentration, blood was taken from caudal artery at 1, 3, 5 hr and the animal were sacrificed 24 hr after ethanol ingestion. From 1 to 5 hr, health drink pre- or postdosing significantly decreased the ethanol levels in the blood. The acetaldehyde concentration showed decrement in health drink group and other company product group. The activities of ethanol, alcohol dehydrogenase and aldehyde dehydrogenase measured at postdosing, were also not altered by the administration of health drink. Alanine aminotransferase and aspartate aminotransferase activities showed unaltered resulted in all experimental groups compared with the normal group. These results suggest that oral intake of health drink containing Hovenia dulcis Thunb is effective on elimination of ethanol-induced hangover.

숙취해소용 음료로 개발된 건강음료를 각각 알코올(5 g/kg B.W, 40%) 투여 30분 전과 후에 경구적으로 섭취시키고(10 mL/kg) 시간(1, 3 및 5)에 따라 미동맥으로 채혈하여 혈액 중 알코올 농도와 아세트알데히드 농도, 간 조직 중 알코올 대사효소 alcohol dehydrogenase (ADH) 및 aldehyde dehydrogenase(ALDH)의 활성과 간기능 지표 효소(ALT, AST)의 활성 변동을 측정 비교하여 다음과 같은 결과를 얻었다. 알코올 투여 30분 전에 건강음료를 공급하였을 때 혈액 중 알코올 농도는 알코올 투여 1시간 후부터 모든 군에서 급격하게 감소하였으며 알코올 투여 5시간째에 알코올 대조군(EC)에 비해 건강음료 투여군(BE)은 48.4%정도 감소하는 경향을 나타내었다. 또한 아세트알데히드 농도는 알코올 대조군(EC)에 비해 건강음료 투여군(BE)은 15.6%, 타사제품 투여군(P)은 20.3% 낮았다. 알코올 투여 30분 후 숙취해소 음료를 공급하고 5시간 경과 후 건강음료 투여군(AE)의 알코올 농도는 알코올 대조군(EC)에 비해 65.2% 낮은 수치를 나타내었다. 아세트알데히드 농도는 알코올 대조군에 비해 건강음료 투여군(AE)은 36.4% 낮은 0.21 mg/dL 타사제품투여군(P)은 24.2% 낮은 0.25 mg/dL를 나타내었다. 간 조직 중 ADH 활성은 정상군과 알코올을 섭취 한 모든 실험군 사이에 별다른 변동을 관찰할 수 없었다. 숙취해소 음료의 1회 섭취와 체중 1 kg당 5 g의 알코올 1회 투여가 알코올 대사 효소의 활성에 영향을 미치지 못함을 시사하고 있다. 혈청 ALT, AST 활성은 정상군과 알코올 투여 실험군간에 유의적인 차이를 보이지 않았으며 또 건강음료의 음용이 정상적인 간 기능에 영향을 미치지 않는 결과를 볼 때 안전성이 인정된다고 생각된다.

References

  1. Kim JH, Min SS, Kim SH, Hong HD, Kim JS, Kim SU. Effect of arrowroot flower (Puerariae flos) extract on lowering of ethanol concentration in rat blood. Agri Chem Biotechnol 38: 549-553,1995
  2. Kim YC, Park SH, Lee MG. Effect of glutamate on the blood concentrations of ethanol in healthy adults. Yakhak Hoeji 37: 549-553,1993
  3. Lee MK, Kim YG, An SW, Kim MH, Lee JH, Lee HY. Biological activity of Hovenia dulcis Thunb, Korean J Medicinal Crop Sci 7:185-192,1999
  4. An BJ, Lee JT. Isolation and characterization of angiotensin converting enzyme inhibitors from Gamellia sinensis L. and their chemical structure detennination. Food Sci Biotechnol 8: 285-289,1999
  5. Mssayuki Y, Murakami T. Absolute stereostructures of new dihydroflavonols, Hovenitins I, II and III, isolated from hovenia semen seu fructus of Hovenia dulcis Thunb. Chem. Pharm Bull 117: 108-118, 1996
  6. Cho JY, Moon JH, Park KH. Isolation and identification of 3-methoxy-4- hydroxybenzoic acid and 3-methoxy-4-hydroxycinnamic acid from hot water extracts of Hovenia dulcis Thunb, and confirmation of their antioxidative and antimicrobial activity. Korean J Food Sci Technol 32: 1403-1408, 2000
  7. Halsted CH. Alcoholism and malnutrition introduction to the symposium. Am J clin Nutr 33: 2705, 1980
  8. Muller A, Sies H. Alcohol, aldehyde and lipid peroxidation : Current nortions. Alcohol Alcohol 22: 67, 1987
  9. Forsander OA. Raihi Niels CR. Metabolites produced in the liver during alcohol oxidation. J Biol Chem 235: 34-36, 1960
  10. Rahwan RG. Speculations on the biochemical pfwmacology of ethanol. Life Sci 15: 617, 1974 https://doi.org/10.1016/0024-3205(74)90502-5
  11. Nanji AA, Zakim D. Alcoholic liver disease. In Hepatology. Zakim D. and Boyer T. (eds.), Saunders, Philadelphia, 3rd ed 3: 891,1996
  12. Mezey E. Metabolic effects of alcohol. Fed Proc 44:134,1985
  13. Swift R, Davidson D. Alcohol hangover, mechanisms and mediators. Alcohol Health Res World 22: 54, 1998
  14. Kato S, Kawase T, Alderman J, Inatomi N, Liber CS. Role of xanthine oxidase in ethanol-induced lipid peroxidation in rat. Gastroenterol 98: 203, 1990 https://doi.org/10.1016/0016-5085(90)91311-S
  15. Bucher T, Redetzki H. Eine spezifische photometrische bestimmung von athylakohol auf fennentativen wege. Klin Wochenschr 29: 615,1951 https://doi.org/10.1007/BF01485653
  16. Bergmeyer HU. Methods of enzymatic analysis Academic-Press, New York. 28, 1974
  17. Koivula T, Koivusalo M. Different from of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochem Biophs ACTA 397: 9,1975 https://doi.org/10.1016/0005-2744(75)90174-6
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 193: 265, 1951
  19. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase, Am J Clin Pathol 28: 8, 1957
  20. Karmen A. A note on the spectrophotometric assay of glutamic oxaloacetic transaminase in human blood serum. J Clin Invest 34:131, 1955
  21. Sakai, K, T. Yamane, Y. Saitoh, C. Ikawa, and T. Nishihata. Effect of water extracts of crude drugs in decreasing blood alcohol concentrations in rats, Chem Pharm Bull 35: 4597-4604,1987 https://doi.org/10.1248/cpb.35.4597
  22. Kim KW, Yang JS, Lee JS, Cho YS, Kang SK, Chung HK. Activity of alcohol dehydrogenase and ethanol acetaldehyde levels in normal adults blood. Kor Ind Hyg Assoc J 4: 240, 1994
  23. An SW, Kim YG, Kim MH, Lee BI, Lee SH, Kwon HI, Hwang B, Lee HY. Comparison of Hepatic Detoxification activity and reducing Serum Alcohol concentration of Hovenia dulsis THUNB and Alnus japonica Steud. Korean J Medicinal Crop Sci 7(4): 263-268,1999
  24. Kim MH, Park CK. Inhibition of ethanol absorption by Rbodiala sachalinensis in rats. Arch Pharm Res 20: 432, 1997 https://doi.org/10.1007/BF02973935
  25. Sakai K, Saitoh Y, Ikawa C, Nishihata T. Effect of water extracts of aloe and some herbs in decreasing blood ethanol concentration in rats. Chem Pharm Bull (Tokyo) 37: 155,1989 https://doi.org/10.1248/cpb.37.155
  26. Bursch W, Schulte HR. Cytoprotective effect of the prostacyclin derivative, Hoprost aganinst live cell death induceded by the hepatotoxins CC14 and bromobenzen. Klin Wochenschr 7: 47, 1986