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Expression of Mosquitocidal Bacillus sphaericus Binary Toxin and B. thuringiensis cry11B Genes in B. thuringiensis 407

  • Park, Hyun-Woo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.185-189
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    • 2001
  • Wild type Bacilus thuringiensis subsp. israelensis and B. sphaericus toxins have been used separately as active in ingredients for bacterial insecticides to control mosquito larvae due to their comparable toxicity to chemical insecticides. Cry11B, recently cloned from B. thuringiensis subsp. jegathesan, shows higher toxicity against three major species of mosquito larvae than Cry11A, one of the major component of B. thuringiensis subsp. israelensis inclusion body. To determine whether the combination of cry11B and B. sphaericus binary toxins is as toxic as B. thuringiensis subsp. israelensis parental strain, cry11B and B. sphaericus binary toxins genes were co-expressed as an operon using cytlA promoters/STAB-SD hybrid expression system in B. thuringiensis subsp. israelensis acrystalliferous strain 4Q7. However, unexpectedly, B. sphaericus binary toxins were barely produced, whereas relatively large amount of Cry11B was produced. When this strain was grown in four different media, NB+G and Peptonized Milk produced more toxin proteins and spores per unit of media than GYS and G-Tris. Toxicity of this strain against fourth instar Culex quinquefasciatus was ranged from of 8.3 to 45.7 ng/ml, with NB+G culture being the highest, and GYS culture was the lowest.

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Unity in HIV-1 Sequence Diversity: Identification and Characterization of Korean Clade in HIV-1 Isolated from Korean

  • Lee, Chan-Hee
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2006.05a
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    • pp.129-131
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    • 2006
  • Through molecular phylogenetic analysis using the nef gene sequences of HIV-l isolated from Korean registered in the NCBI GenBank together with 41 reference strains and 94 foreign isolates, we verified that most (${\sim}80%$) of Korean isolates belonged to subtype B and 78% of subtype B were clustered together exclusively of foreign isolates, and this cluster was named Korean clade subtype B ($K_cB$). Similarity study suggested that the $K_cB$ cluster was more homogeneous than and clearly distinctive from the non-Korean subtype B ($NK_cB$). Comparison of the consensus amino acid sequences of the $K_cB\;or\;NK_cB$ revealed characteristic $K_cB$ signature amino acid pattern comprised of 13 amino acid residues. The $K_cB$ signature amino acid residues were critical in separating the $K_cB$ ftom the $NK_cB$, since substitution of the $NK_cB$ sequences with $K_cB$ signature amino acids relocated them to the Koran clade, and vice versa. Synonymous and nonsynonymous substitution rate study suggested positive selection event for the $K_cB$.

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Model of the ebXML-based BPSS Conformance Validation in B2B Enviroment (B2B 환경에서 ebXML 기반 BPSS 적합성 검증 모델)

  • Shin, Myeong-Sook;Choi, Jong-Sik;Ahn, Seong-Soo;Lee, Joon
    • The Journal of the Korea institute of electronic communication sciences
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    • v.2 no.2
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    • pp.113-117
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    • 2007
  • Recently B2B electronic transactions which go through an Internet being diffused, according to support the Internet business of the form which is various the techniques for are appearing. B2B electronic transaction systems respect B2B transactions consequently to control the system of selfish bell environmnet and various information, must exchange. But currently the tentative tool for the verification of BPSS is the condition which is wholly lacking with the domestic foreign enemy. In this paper the suitability ebMS standard verification entry written in conjunction with ebXML BPSS document the suitability of the case, depending on the item to evaluate whether the BPSS model, suggesting that fitness will be verified.

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Demand Forecasting for B2B Electronic Products : The Case of Personal Computer Market (B2B 전자제품 수요예측 모형 : PC시장 사례)

  • Moon, Jeongwoong;Chang, Namsik;Cho, Wooje
    • Journal of Information Technology Services
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    • v.14 no.4
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    • pp.185-197
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    • 2015
  • As the uncertainty of demand in B2B electronics market has increased, firms need a strong method to estimate the market demand. An accurate prediction on the market demand is crucial for a firm not to overproduce or underproduce its goods, which would influence the performance of the firm. However, it is complicated to estimate the demand in a B2B market, particularly for the private sector, because firms are very diverse in terms of size, industry, and types of business. This study proposes both qualitative and quantitative demand forecasting approaches for B2B PC products. Four different measures for predicting PC products in B2B market with consideration of the different PC uses-personal work, common work, promotion, and welfare-are developed as the qualitative model's input variables. These measures are verified by survey data collected from experts in 139 firms, and can be applied when individual firms estimate the demand of PC goods in a B2B market. As the quantitative approach, the multiple regression model is proposed and it includes variables of region, type of industry, and size of the firm. The regression model can be applied when the aggregated demand for overall domestic PC market needs to be estimated.

Endoscopic ultrasound-guided hepaticogastrostomy by puncturing both B2 and B3: a single center experience

  • Moaz Elshair;Kazuo Hara;Nozomi Okuno;Shin Haba;Takamichi Kuwahara;Asmaa Bakr;Abdou Elshafei;Mohamed Z. Abu-Amer
    • Clinical Endoscopy
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    • v.57 no.4
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    • pp.542-546
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    • 2024
  • Endoscopic ultrasound-guided hepaticogastrostomy (EUS-HGS) through ducts B2 or B3 is effective in most patients with biliary obstruction, because B2 and B3 commonly join together. However, in some patients, B2 and B3 do not join each other due to invasive hilar tumors; therefore, single-route drainage is insufficient. Here, we investigated the feasibility and efficacy of EUS-HGS through both B2 and B3 simultaneously in seven patients. We decided to perform EUS-HGS through both B2 and B3 to achieve adequate biliary drainage because these two ducts were separate from each other. Here, we report a 100% technical and overall clinical success rate. Early adverse effects were closely monitored. Minimal bleeding was reported in one patient (1/7) and mild peritonitis in one patient (1/7). None of the patients experienced stent dysfunction, fever, or bile leakage after the procedure. EUS-HGS through both B2 and B3 simultaneously is safe, feasible, and effective for biliary drainage in patients with separated ducts.

Deficiency in Opu Systems Imparts Salt-Sensitivity to Weizmannia coagulans

  • Tao Kim;Sojeong Heo;Jong-Hoon Lee;Do-Won Jeong
    • Journal of Microbiology and Biotechnology
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    • v.34 no.7
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    • pp.1443-1451
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    • 2024
  • Weizmannia coagulans can be used as a starter strain in fermented foods or as a probiotic. However, it is salt-sensitive. Here, W. coagulans genomes were compared with the genomes of strains of Bacillus species (B. licheniformis, B. siamensis, B. subtilis, and B. velezensis) that were isolated from fermented foods and show salt tolerance, to identify the basis for the salt-sensitivity of W. coagulans. Osmoprotectant uptake (Opu) systems transport compatible solutes into cells to help them tolerate osmotic stress. B. siamensis, B. subtilis, and B. velezensis each possess five Opu systems (OpuA, OpuB, OpuC, OpuD, and OpuE); B. licheniformis has all except OpuB. However, W. coagulans only has the OpuC system. Based on these findings, the opuA and opuB operons, and the opuD and opuE genes, were amplified from B. velezensis. Expression of each of these systems, respectively, in W. coagulans increased salt-tolerance. W. coagulans expressing B. velezensis opuA, opuD, or opuE grew in 10.5% NaCl (w/v), whereas wild-type W. coagulans could not grow in 3.5% NaCl. The salt resistance of B. subtilis was also increased by overexpression of B. velezensis opuA, opuB, opuD, or opuE. These results indicate that the salt-susceptibility of W. coagulans arises because it is deficient in Opu systems.

FIBREWISE INFINITE SYMMETRIC PRODUCTS AND M-CATEGORY

  • Hans, Scheerer;Manfred, Stelzer
    • Bulletin of the Korean Mathematical Society
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    • v.36 no.4
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    • pp.671-682
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    • 1999
  • Using a base-point free version of the infinite symmetric product we define a fibrewise infinite symmetric product for any fibration $E\;\longrightarrow\;B$. The construction works for any commutative ring R with unit and is denoted by $R_f(E)\;l\ongrightarrow\;B$. For any pointed space B let $G_I(B)\;\longrightarrow\;B$ be the i-th Ganea fibration. Defining $M_R-cat(B):= inf{i\midR_f(G_i(B))\longrihghtarrow\;B$ admits a section} we obtain an approximation to the Lusternik-Schnirelmann category of B which satisfies .g.a product formula. In particular, if B is a 1-connected rational space of finite rational type, then $M_Q$-cat(B) coincides with the well-known (purely algebraically defined) M-category of B which in fact is equal to cat (B) by a result of K.Hess. All the constructions more generally apply to the Ganea category of maps.

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DC Pulsed Magnetron Sputtering 법으로 제조된 B-C 박막과 B-C/DLC 다층막의 물성에 관한 연구

  • Kim, Gang-Sam;Jo, Yong-Gi
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.02a
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    • pp.311-311
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    • 2012
  • Boron carbide (B-C) 박막은 높은 경도, 열적 안전성, 화학적 안전성이 우수한 하드 코팅 소재로 사용되고 있다. 우수한 특성을 가지는 B-C 박막에 대한 연구는 B4C 비전도성 타겟을 이용하여 RF Sputtering 법으로 증착 공정변수에 대해서 박막의 물성에 관해 일부 연구자들이 진행하였으나, Pulsed dc margnetron sputtering 법으로 증착 공정변수에 대한 물성의 연구는 미진하였다. 반면에, DLC 박막은 우수한 특성을 가지는 하드 코팅 소재이나 400도 이상에서는 내열성이 떨어지는 단점을 가지고 있다. 연구에서는 B-C 박막의 내열성이 우수한 특성을 이용하여 DLC 박막의 내열성을 높이기 위한 목적으로 B-C 박막과 DLC 박막을 다층막으로 제조함으로서 DLC 박막을 구조적으로 안정화를 시키고자 하였다. 그리고 비전도성 B4C 타겟으로 Pulsed dc 마그네트론 스퍼터링법을 이용하여 증착기술을 개발하기 위해서 공정압력과 인가전력에 따른 B-C 박막을 제조하여 그 물성을 조사하였고, B-C/DLC 다층막을 제조하여 DLC 박막의 내열성을 증가시키고자 하였다. B-C 박막과 B-C/DLC 다층막의 경도와 탄성율은 나노인덴테이션과 마이크로 비커스를 이용하였으며, 박막의 성장구조와 박막의 구조를 조사하기 위해 SEM과 FTIR 및 XRD 을 이용하여 측정하였다.

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Reaction Synthesis and Mechanical Properties of $B_4C$-based Ceramic Composites

  • Han, Jae-Ho;Park, Sang-Whan;Kim, Young-Do
    • Proceedings of the Korean Powder Metallurgy Institute Conference
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    • 2006.09b
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    • pp.1080-1081
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    • 2006
  • In this investigation, $B_4C$ based ceramic composites were fabricated by in-situ reaction hot pressing using $B_4C$, TiC SiC powder as starting materials. The reaction synthesized composites by hot pressing at $1950^{\circ}C$ was found to posses very high relative density. The reaction synthesized $B_4C$ composites comprise $B_4C$, $TiB_2$, SiC and graphite by the reaction between TiC and $B_4C$. The newly formed $TiB_2$ and graphite was embedded both inside grain and at grain boundary $B_4C$. The mechanical properties of reaction synthesized $B_4C-TiB_2-SiC$-graphite composites were more enhanced compared to those of monolithic $B_4C$.

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ISOGONAL AND ISOTOMIC CONJUGATES OF QUADRATIC RATIONAL Bézier CURVES

  • Yun, Chan Ran;Ahn, Young Joon
    • The Pure and Applied Mathematics
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    • v.22 no.1
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    • pp.25-34
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    • 2015
  • In this paper we characterize the isogonal and isotomic conjugates of conic. Every conic can be expressed by a quadratic rational B$\acute{e}$zier curve having control polygon $b_0b_1b_2$ with weight w > 0. We show that the isotomic conjugate of parabola and hyperbola with respect to ${\Delta}b_0b_1b_2$ is ellipse, and that the isotomic conjugate of ellipse with the weight $w={\frac{1}{2}}$ is identical. We also find all cases of the isogonal conjugate of conic with respect to ${\Delta}b_0b_1b_2$. Our characterizations are derived easily due to the expression of conic by the quadratic rational B$\acute{e}$ezier curve in standard form.