• 미생물학회지
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• 제20권4호
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• pp.189-194
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• 1982

Acid protease produced from Aspergillus tubingensis was pruified by ethanol fractionation, dialysis, and DEAE cellulose column chromatography. As a result of purification its specific activity increased to 5.4 times, and percent recovery was 39. The kinetic constants of the enzyme were studied. Km and Vmax was $1.5{\times}10^{-7}M\;and\;0.11{\Delta}O.D/min$ , respectively, when casein was used as substrate. The order of Km value of several proteins is : casein

• 한국식물병리학회지
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• 제10권3호
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• pp.197-210
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• 1994

This experiment was carried out to study the cause of degeneration and rot of cultivated mushroom. Among 597 bacterial isolates derived from the rots of Button mushroom (Agaricus bisporus), Oyster mushroom (Pleurotus ostreatus) and Oak mushroom (Lentinus edodes) collected from markets of 5 cities (Seoul, Suwon, Taegu, Pohang and Pusan) in Korea (1991~1993), 111 bacterial isolates (18.5%) were proved as pathogenic bacteria. These pathogenic bacteria causing bacterial rots of cultivated mushrooms were identified as Pseudomonas tolasii, P. agarici, and Eriwinia sp., and the main causal bacteria were P. tolaasii. P. fluorescens and Klebsiella plenticola were confirmed as saprophytic non-pathogenic bacteria. One hundred fifty nine isolates (Group No. 39) of the 486 saprophytic bacterial isolates were classified as P. fluorescens, and this species was most often found rot area of cultivated mushrooms. P. tolaasii, the causal organism of bacterial blotch, was classified into two groups; One group can be differentiated from the other by the formation of white precipitation band by white line reacting organisms of Pseudomonas Agar F media. P. tolaasii attacked the cultivated mushrooms relatively well at lower incubation temperature such as 5$^{\circ}C$, but P. agarici rarely attack at below 1$0^{\circ}C$. The temperature for the infection commercial cultivated mushrooms by P. agarici was higher than that of P. tolaasii. Optimum temperature for the infection of mushrooms by P. tolaasii and P. agarici were 2$0^{\circ}C$ and $25^{\circ}C$, respectively.

• Journal of information and communication convergence engineering
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• 제12권1호
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• pp.33-38
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• 2014

This paper investigates the shaping operation problem introduced by Callahan et al., namely the k-fragility maximization problem (k-FMP), whose goal is to find a subset of personals within a terrorist group such that the regeneration capability of the residual group without the personals is minimized. To improve the impact of the shaping operation, the degree centrality of the residual graph needs to be maximized. In this paper, we propose a new greedy algorithm for k-FMP. We discover some interesting discrete properties and use this to design a more thorough greedy algorithm for k-FMP. Our simulation result shows that the proposed algorithm outperforms Callahan et al.'s algorithm in terms of maximizing degree centrality. While our algorithm incurs higher running time (factor of k), given that the applications of the problem is expected to allow sufficient amount of time for thorough computation and k is expected to be much smaller than the size of input graph in reality, our algorithm has a better merit in practice.

• 정보처리학회논문지C
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• 제11C권6호
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• pp.765-772
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• 2004

멀티캐스트는 송신자가 많은 수신자들에게 동시에 데이터를 전송하므로 송신자의 자원을 절약하고 네트워크의 점유율을 낮춤으로 효율성을 제공하는 통신 기술이나 여러 수신자들이 같은 그룹 주소를 사용하므로 공격을 받을 위험이 크다. 따라서 멀티캐스트 스트림에 대한 인증 및 서명은 중요한 문제이다. 본 논문에서는 패킷을 전송한 송신자의 신원을 확인하고 데이터가 변조되지 않았음을 확인하는 소스 인증 방법으로 다중 체인 인증기범을 제안하였다. 제안한 기법은 부인 방지를 제공하고 여러 패킷에 대한 전자서명으로 인증함으로써 오버헤드론 줄이며 지연없이 이루어지므로 실시간 멀티미디어 서비스에 사용할 수 있다.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.62-68
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• 2014

Coptis chinensis 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase (HOMT), an essential enzyme in the berberine biosynthetic pathway, catalyzes the methylation of 3'-hydroxy-N-methylcoclaurine (HMC) producing reticuline. A 3D model of HOMT was constructed by homology modeling and further subjected to docking with its ligands and molecular dynamics simulations. The 3D structure of HOMT revealed unique structural features which permitted the methylation of HMC. The methylation of HMC was proposed to proceed by deprotonation of the 4'-hydroxyl group via His257 and Asp258 of HOMT, followed by a nucleophilic attack on the SAM-methyl group resulting in reticuline. HOMT showed high substrate specificity for methylation of HMC. The study evidenced that Gly117, Thr312 and Asp258 in HOMT might be the key residues for orienting substrate for specific catalysis.

• Bulletin of the Korean Chemical Society
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• 제15권6호
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• pp.473-477
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• 1994

Second-order rate constants have been measured spectrophotometrically for the nucleophilic substitution reactions of aryl substituted benzenesulfonates $(1,\;X-C_6H_4SO_2-OC_6H_4-Y)$ with aryloxides $(Z-C_6H_4O^{-})$ and ethoxide $(EtO^-)$ in absolute ethanol at $25^{circ}C$. The nucleophilicity of aryloxides increases with increasing electron donating ability of the substituent (Z) on aryloxides, and results in a good Hammett correlation with $\sigma^{-}$ constant. The reactivity of 1 toward aryloxides and ethoxide shows also significant dependence on the electronic nature of the substituent X and Y. Large positive ${\sigma}_{acyl}$ values have been obtained for the reaction of 1 with phenoxide and ethoxide, indicating that the leaving group departure is little advanced at the transition-state of the rate-determining step. This has been further supported from the fact that ${\sigma}^-$ constant gives extremely poor Hammett correlation, while ${\sigma}^0$ does reasonably good correlation for the reaction of 1 with ethoxide. Thus, the present sulfonyl-transfer reaction is proposed to proceed via a ratedetermining attack of nucleophile to the sulfur atom of 1 followed by a fast leaving group departure.

• Bulletin of the Korean Chemical Society
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• 제19권11호
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• pp.1211-1216
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• 1998

Bis(diethyldithiocarbamato)ioxomolybdenum(VI), cis-MoO2(S2CNEt2)2, 1, reacted with chlorotrimethylsilane (Me33SiCl) to give a seven-coordinate, pentagonal bipyramidal complex MoOC12(S2CN]Et2)2, 3, in which the oxo ligand is trans to the chloride ligand and the two chloride ligands are mutually cis. The monooxo molybdenum complex bis(diethyidithiocarbamato)oxomolybdenum(IV), MoO(S2CNEt2)2, 2, reacted with phenyl isocyanate (PhNCO) to give an Mo dimer MO2{μ-N(CONPh)Ph}(μ-NPh)(NPh)2(S2CNEt2)2, 4, which contains an Mo-Mo bond, two diethyldithiocarbamato ligands, two terminal imido (NPh) ligands, and two bridging hnido (NPh) ligands. One of the two bridging NPh ligands seemed to have been attacked by the electrophilic phenyl isocyanate carbon, which suggests that the bridging imido NPh ligand is more nucleophilic than the terminal one. Crystallographic data for 3: monoclinic space group P21/c, a=8.908(l) Å, b=17.509(3) Å, c=12.683(2) Å, β=110.15(1)°, Z=4, R(wR2)=0.0611(0.1385). Crystallographic data for 4-THF: orthorhombic space group P212121, a=17.932(4) Å, b=22.715(5) Å, c=11.802(3) Å, Z=4, R(wR2)=0.0585(0.1286).

• 정보보호학회논문지
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• 제15권4호
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• pp.61-70
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• 2005

We propose and implement a flexible secure peer-to-peer(P2P) file sharing scheme which can be used for data sharing among closed user group (CUG) members. When a member wants to share data, notification messages are sent to the members with whom the member wants to share data. Each notification message includes one-time password encrypted with the receiver's public key. A member who received the notification message can download the data by using the one-time password. The proposed scheme provides selective sharing, download confirmation and efficient storage management. In terms of security, the proposed scheme supports authentication, entity privacy, replay attack protection and disguise prevention. We also implement the proposed system and find that the system is very useful among P2P service of closed user groups.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.143-146
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• 2008

본 논문에서는 3D FPS 게임에서 다수의 NPC(Non Player Character)들이 player를 대상으로 접근할 때 유사한 속성을 가진 NPC들을 객체별 그룹핑을 형성하게 하고, 그룹핑 된 NPC들의 위험도를 판단하여 player가 적절히 대응할 수 있는 방안을 제시한다. NPC들의 그룹핑을 위한 각 속성은 게임의 특성에 따라 별도로 정한다. 따라서 속성에는 NPC가 가지는 행동패턴, 공격패턴, 무기패턴 등의 다양한 요소로 형성되고 이러한 요소들이 하나의 그룹에 대한 속성이 된다. NPC들이 그룹으로 형성되는 크기는 NPC 들이 가지는 속성들의 개수와 동일하게 설정하고 이동을 하면서 자신과 유사한 속성을 가진 다른 NPC들과 즉석에서 그룹을 형성하도록 한다. 또한 형성된 그룹들 중에 우선순위를 고려하여 player의 공격 여부를 판단한다. 제안된 방법은 변화가 많은 FPS 게임에서 player와 NPC 들이 효과적으로 플레이 할 수 있을 것으로 기대한다.

• Bulletin of the Korean Chemical Society
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• 제32권11호
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• pp.3947-3951
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• 2011

The nucleophilic substitution reactions of Y-aryl ethyl chlorothiophosphates with X-pyridines are studied kinetically in acetonitrile at $55.0^{\circ}C$. The Hammett and Bronsted plots for substituent X variations in the nucleophiles exhibit biphasic concave upwards with a break point at X = 3-Me. The substituents of X = 4-CN and 4-Ac show great positive deviations from both the Hammett and Bronsted plots. The Hammett plots for substituent Y variations in the substrates exhibit biphasic concave upwards with a minimum point at Y = H. The obtained values of the cross-interaction constants (${\rho}_{XY}$) are all in spite of the biphasic free energy correlations for both substituent X and Y variations, since the ${\rho}_X$values with both the strongly and weakly basic pyridines are almost constant. A stepwise mechanism with a rate-limiting leaving group departure from the intermediate is proposed where the distance between X and Y does not vary from the intermediate to the second transition state. A frontside attack is proposed with the strongly basic pyridines based on the considerably great magnitudes of ${\rho}_X$ and ${\beta}_X$ values and a backside attack is proposed with the weakly basic pyridines based on the relatively small magnitudes of ${\rho}_X$ and ${\beta}_X$. The positive deviations of the two strong ${\pi}$-acceptor parasubstituents, X = 4-Ac and 4-CN, from both the Hammett and Bronsted plots are rationalized by the great extents of bond formation and breaking.