• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1670-1674
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• 2007

Duffy binding protein (DBP) plays a critical role in Plasmodium vivax invasion of human red blood cells. We previously reported a single-chain antibody fragment (scFv) that was specific to P. vivax DBP (PvDBP). However, the stabilization and the half-life of scFvs have not been studied. Here, we investigated the effect of PEGylated scFvs on their biological activity and stability in vitro. SDS-PAGE analysis showed that three clones (SFDBII-12, -58, and -92) were formed as monomers (about 70 kDa) with PEGylation. Clone SFDBII-58 gave the highest yield of PEGylated scFv. Binding analysis using BIAcore between DBP and scFv showed that both SFDBII-12 and -58 were decreased approximately by two folds at the level of binding affinity to DBP after PEGylation. However, the SFDBII-92 clone still showed a relatively high level of binding affinity ($K_D=1.02{\times}10^{-7}\;M$). Binding inhibition assay showed that PEGylated scFv was still able to competitively bind the PvDBP and playa critical role in inhibiting the interactions between PvDBP protein expressed on the surface of Cos-7 cells and Duffy receptor on the surface of erythrocytes. When both scFvs and their PEGylated counterparts were exposed to trypsin, scFv was completely degraded only after 24 h, whereas 35% of PEGylated scFvs remained intact, maintaining their stability against the proteolytic attack of trypsin until 72 h. Taken together, these results suggest that the PEGylated scFvs retain their stability against proteolytic enzymes in vivo, with no significant loss in their binding affinity to target antigen, DBP.

• Aerospace Engineering and Technology
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• v.2 no.1
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• pp.171-181
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• 2003

The nose fairings of KSR-III are designed to be separated from the rocket by explosive force at the mission altitude to expose the payload. Adequate amount of separation force should be imposed to allow safe separation without collision between the fairings and the rocket, and the separation device was designed for the separation at very high altitude where almost no air load was expected. As the development of KSR-III goes on, several design changes have made and lower separation altitude of 45km is expected as a result. Under these circumstances, it is required to determine if the nose fairings can be separated without collision with much severer air load than for the design condition. In this study, the 6-DOF motion analysis program, PASEM, which was developed to predict the strap-on booster separation, is modified to simulate the pivotal motion of the fairings at early stages of separation. The accuracy of pivot motion simulation is validated by comparison with the results of ground test and the accurate separation conditions are deduced from it. Trajectory simulations are performed to see if separation without collision is possible with varying angle of attack, direction of gravity, and the effect of gust. It is also found that reducing the separation angle of the clamshell hinge from 60 degrees to 40 degrees can enhance separation safety and separation at lower altitude of 40km can be done without collision.

• Journal of Life Science
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• v.19 no.3
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• pp.343-348
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• 2009

Magnaporthe oryzae, a major cause of rice blast, is one of the most destructive plant fungal pathogens. Secretion of reactive oxygen species (ROS) during the infection phase of plant pathogenic fungus plays a key role in the defense mechanism of a plant. ROS causes oxidative damage and functional modification to the proteins in a pathogenic fungus. Methionine, especially, is a major target of ROS, which oxidizes it to methionine sulfoxide. To survive from the attack of ROS, plant pathogenic fungus has antioxidative systems - one example would be methionine sulfoxide reductase B (MSRB), which reverses the oxidative alteration of methionine to methionine sulfoxide. In the present study, identification and molecular characterization of the MSRB gene in M. oryzae KJ201 were investigated. The MSRB gene was amplified by PCR from the M. oryzae KJ201 genomic DNA. The copy number of MSRB in the genome of M. oryzae KJ201 was identified by Southern blot analysis, which revealed that the gene exists as a single copy. To study the molecular function of an MSRB gene, the expression level of the MSRB gene was assayed with hydrogen peroxide treatment by Northern blot analysis and RT-PCR. The expression of the MSRB gene was increased by treatment of hydrogen peroxide, without significant correlation to hydrogen peroxide concentrations. These results indicate that the MSRB gene in M. oryzae KJ201 could contribute to protection against plant defense compounds such as ROS and offer a novel strategy for the control of rice blast.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.10
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• pp.137-147
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• 2007

In the host-based Mobile IPv6, a mobile node is responsible for doing the signaling to its home agent to enable session continuity as it moves between subnets. To remove the mobile node's signalling processing load, the network-based Mobile IPv6 has been proposed recently. It allows session continuity for a mobile node without its involvement in mobility management. The proxy mobility agent in the network performs the signaling and does the mobility management on behalf of the mobile node. However, to make secure communications for a mobile node, security mechanisms against diverse attacks should be adopted. To do this, first of all security threats to the network-based Mobile IPv6 should be also identified and analyzed. Potential attack objectives may be to consume network services at the cost of a legitimate mobile node and, eavesdropping and fabrication of user traffic through interception of a mobile node's communications. This paper identifies and discusses security threats to the network-based Mobile IPv6 in details. The results of threats analysis are limited to threats that are peculiar to the network-based Mobile IPv6 except threats to IPv6 in general.

• Korean Security Journal
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• no.17
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• pp.33-51
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• 2008

According to dictionary, the meaning of protection is "guard and protect" that means protecting the Protectee's safety in case of sudden attack or various accident and Security means all protecting activity including Protectee and place where he is in or will be as comprehensively meaning of safe. As you see in the definition, Protection and security is the act to protect or will to protect from a security-threat act. A security-threat act can be discussed in the range of the concept of a criminal act in Criminal Law. A security-threat act is based on criminal act in Criminal Law, we are going to review such a security-threat act in a point of view in a sphere of learning in today's remarkable a brain-neuro science and cognitive science based on cognitive psychology, and to use an analysis on such a security-threat act to make a foundation for a establishment of the scientific preventive social security countermeasure. To do so, First of all we are going to review a security-threat act based on criminal act in Criminal Law in a point of protection police logic view. Next, we are going to introduce how cognitive science understand about act of man before we analyse a threat act as one of an act of man in cognitive science point of view. Finally, we are going to discuss the need of cognitive scientific analyse in order to establish the Scientific Preventive Social-security Countermeasure at the same time we are going to analyse a threat act in a cognitive scientific view.

• Korean Security Journal
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• no.15
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• pp.221-242
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• 2008

Protection is to maximize the protectee's physical security by minimizing the threats and minimizing the damages caused by the threats. From this point of view, the assassination case of Israel's former Premier Yitzhak Rabin who devoted himself to proceed peace process in Middle East, on November 4, 1995, gives us a lot of lessons on protection. At that time, Israeli society was chaotic with anti-Rabin and anti-government demonstrations which is against Rabin's peace process, and they openly threatened to assassinate Premier Rabin. However, Shin Bet, Israeli Protective Agency, didn't reacted against the assassination threats effectively. Also they have to try to secure friendly public to widen the width of selection in case of crisis and to secure the credibility of the organization. They have to prevent harmful activities in advance by securing wide intelligence networks to collect and analyze various protective intelligences and preparing proper protective countermeasures. Intelligence is a basic element in protection and any protective measures not supported by intelligence activities are not useful and effective. Potential attackers study their target thoroughly and make an elaborate plan before their attack. Advance team is required to conduct security measures on a basis of analysis of vulnerability and threat factors, and close protection team should be prepared for the changing situations with crisis-consciousness. Is security, 100% perfection is not possible. However, Shin Bet failed to analyze and approach to the existing threats. They forgot the fact that security environment reflects social situations. Protection agents should be conscious of the fact that today is different from yesterday. So we have to expect what is not expected when not expected.

• Advances in concrete construction
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• v.10 no.2
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• pp.171-183
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• 2020

The main objective of this study is the assessment of the ability of limestone mortars to resist to different chemical attacks. The ability of polypropylene (PP) fibres waste used as reinforcement of these concrete materials to enhance their durability is also studied. Crushed sand 0/2 mm which is a fine limestone residue obtained by the crushing of natural rocks in aggregates industry is used for the fabrication of the mortar. The fibres used, which are obtained from the waste of domestic plastic sweeps' fabrication, have a length of 20 mm and a diameter ranging between 0.38 and 0.51 mm. Two weight fibres contents are used, 0.5 and 1%. The durability tests carried out in this investigation included the water absorption by capillarity, the mass variation, the flexural and the compressive strengths of the mortar specimens immersed for 366 days in 5% sodium chloride, 5% magnesium sulphate and 5% sulphuric acid solutions. A mineralogical analysis by X-ray diffraction (XRD) and a visual inspection are used for a better examination of the quality of tested mortars and for better interpretation of their behaviour in different solutions. The results indicate that the reinforcement of limestone mortar by PP fibres waste is an excellent solution to improve its chemical resistance and durability. Moreover, the presence of PP fibres waste does not affect significantly the water absorption by capillarity of mortar nether its mass variation, when exposed to chloride and sulphate solutions. While in sulphuric acid, the mass loss is higher with the presence of PP fibres waste, especially after an exposure of 180 days. The results reveal that these fibres have a considerable effect of the flexural and the compressive behaviour of mortar especially in acid solution, where a reduction of strength loss is observed. The mineralogical analysis confirms the good behaviour of mortar immersed in sulphate and chloride solutions; and shows that more gypsum is formed in mortar exposed to acid environment causing its rapid degradation. The visual observation reveals that only samples exposed to acid attack during 366 days have showed a surface damage extending over a depth of approximately 300 ㎛.

• Journal of the Society of Naval Architects of Korea
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• v.28 no.2
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• pp.159-173
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• 1991

This paper describes a potential-based panel method formulated for the analysis of a super-cavitating two-dimensional hydrofoil. The method employs normal dipoles and sources distributed on the foil and cavity surfaces to represent the potential flow around the cavitating hydrofoil. The kinematic boundary condition on the wetted portion of the foil surface is satisfied by requiring that the total potential vanish in the fictitious inner flow region of the foil, and the dynamic boundary condition on the cavity surface is satisfied by requiring thats the potential vary linearly, i.e., the tangential velocity be constant. Green's theorem then results in a potential-based integral equation rather than the usual velocity-based formulation of Hess & Smith type. With the singularities distributed on the exact hydrofoil surface, the pressure distributions are predicted with improved accuracy compared to those of the linearized lilting surface theory, especially near the leading edge. The theory then predicts the cavity shape and cavitation number for an assumed cavity length. To improve the accuracy, the sources and dipoles on the cavity surface are moved to the newly computed cavity surface, where the boundary conditions are satisfied again. This iteration process is repeated until the results are converged. Characteristics of iteration and discretization of the present numerical method are much faster and more stable than the existing nonlinear theories. The theory shows good correlations with the existing theories and experimental results for the super-cavitating flow. In the region of small angles of attack, the present prediction shows and excellent comparison with the Geurst's linear theory. For the long cavity, the method recovers the trends of the Wu's nonlinear theory. In the intermediate regions of the short super-cavitation, the method compares very well with the experimental results of Parkin and also those of Silberman.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.6
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• pp.751-759
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• 2007

We have developed several methods for the optimization problem having large-scale and highly nonlinear system. First, step by step method in optimization process was employed to improve the convergence. In addition, techniques of furnishing good initial guesses for analysis using sensitivity information acquired from optimization iteration, and of manipulating analysis/optimization convergency criterion motivated from simultaneous technique were used. We applied them to flow control problem and verified their efficiency and robustness. However, they are based on quasi-Newton method that approximate the Hessian matrix using exact first derivatives. However solution of the Navier-Stokes equations are very cost, so we want to improve the efficiency of the optimization algorithm as much as possible. Thus we develop a true Newton method that uses exact Hessian matrix. And we apply that to the three-dimensional problem of flow around a sphere. This problem is certainly intractable with existing methods for optimal flow control. However, we can attack such problems with the methods that we developed previously and true Newton method.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.29 no.6
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• pp.1243-1257
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• 2019

With the emergence of the IoT environment, various smart devices provide consumers with the convenience and various services. However, as security threats such as invasion of privacy have been reported, the importance of security issues in the IoT environment has emerged, and in particular, the security problem of key management has been discussed, and the PUF has been discussed as a countermeasure. In relation to the key management problem, a protocol using MIPUF has been proposed for the security problem of the group key management system. The system can be applied to lightweight IoT environments and the safety of the PUF ensures the safety of the entire system. However, in some processes, it shows vulnerabilities in terms of safety and efficiency of operation. This paper improves the existing protocol by adding authentication for members, ensuring data independence, reducing unnecessary operations, and increasing the efficiency of database searches. Safety analysis is performed for a specific attack and efficiency analysis results are presented by comparing the computational quantities. Through this, this paper shows that the reliability of data can be improved and our proposed method is lighter than existing protocol.