• BMB Reports
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• v.29 no.5
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• pp.448-454
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• 1996

Bacteriophage ${\phi}FC1$ is a temperate phage which was identified as a prophage in the Enterococcus faecalis KBL703 chromosome. Phage ${\phi}FC1$ integrates into the host chromosome by site-specific recombination. The phage attachment site P (attP) was localized within the 0.65-kb XhoI-HindIII fragment and the nucleotide sequence of the region was determined. An open reading frame (mj1) which adjoined the phage attachment site encoded a deduced protein related to the site-specific recombinase family. The organization of this region was comparable to other site-specific recombination systems. The molecular weight of the expressed MJ1 in E. coli was in good agreement with the predicted 53,537 Da of the mj1 gene product. Elucidation of the phage-specific integration process in this study would provide useful genetic tools such as a chromosomal integration system.

• Industrial Engineering and Management Systems
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• v.8 no.1
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• pp.66-71
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• 2009

This study proposes a new framework for designing disassembly methods. In recent years, environmental problems have become global issues. Recycling of used products or resources is recognized as a matter of significance since it may help reduce the risk of exhausting natural resources. Considering possible exhaustion of limited natural resources in the near future, reuse of products would gain more environmental significance. As yet, it relies hugely on manual disassembly, which labor cost places burden on the total recycling cost. The purpose of this study is to propose a methodology designing for manual disassembly works, and a creation method of a jig. By focusing on parts' connection and attachment relationship, parts are categorized in 5 categories (parent part, joint key part, attaching key part, child part, and independent part) according to the features that parts possess, and 3 kinds of connection relationships (parent part-joint key part connection, parent part-independent part connection and child part-child part connection) are clarified. Connection relationship and attachment relationship charts have also been created, and utilizing them, disassembly orders are settled, and a disassembly jig is devised. The proposed methodology is also applied to a real product and its work time is improved 42% form 31 to 13 seconds.

• Proceedings of the KIEE Conference
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• 1995.07b
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• pp.705-709
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• 1995

The objective of this study is to design a multi-layer neural network which controls the position of excavator's attachment. In this paper, a dynamic controller has been developed based on an error back-propagation(BP) neural network. Since the neural network can model an arbitrary nonlinear mapping, it was used as a commanded feedforward input generator. A PD feedback controller is used in parallel with the feedforward neural network to train the system. The neural network was trained by the current state of the excavator as well as the PD feedback error. By using the BP network as a feedforward controller, no a priori knowledge on system dynamics is need. Computer simulation results demonstrate such powerful characteristics of the proposed controller as adaptation to changing environment, robustness to disturbancen and performance improvement with the on-line learning in the position control of excavator attachment.

• International Journal of Automotive Technology
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• v.8 no.5
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• pp.629-636
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• 2007

This paper describes the process of analyzing vehicle stiffness in terms of frequency band in order to improve vehicle handling. Vehicle handling and ride comfort are highly related to the systems such as suspension, seat, steering, and the car body design. In existing analytical processes, the resonance frequency of a car body is designed to be greater than 25 Hz in order to increase the stiffness of the body against idle vibration. This paper introduces a method for using a band with a frequency lower than 20 Hz to analyze how stiffness affects vehicle handling. Accordingly, static stiffness analysis of a 1g cornering force was conducted to minimize the deformation of vehicle components derived from a load on parts attached to the suspension. In addition, this technology is capable of achieving better performance than older technology. Analysis of how body attachment stiffness affects the dynamic stiffness of a bushing in the attachment parts of the suspension is expected to lead to improvements with respect to vehicle handling and road noise. The process of developing a car body with a high degree of stiffness, which was accomplished in the preliminary stage of this study, confirms the possibility of improving the stability performance and of designing a lightweight prototype car. These improvements can reduce the time needed to develop better vehicles.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.2
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• pp.160-166
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• 2005

In this paper, to overcome drawbacks of FLC a self tuning fuzzy sliding mode controller is proposed, which controls the position of excavator's attachment, which can be regarded as an ill-defined system. It is reported that fuzzy logic theory is especially useful in the control of ill-defined system. It is important in the design of a FLC to derive control rules in which the system's dynamic characteristics are taken into account. Control rules are usually established using trial and error methods. However, in the case where the dynamic characteristics vary with operating conditions, as in the operation of excavator attachment, it is difficult to find out control rules in which all the working condition parameters are considered. Experiments are carried out on a test bed which is built around a commercial Hyundai HX-60W hydraulic excavator. The experimental results show that both alleviation of chattering and performance are achieved. Fuzzy rules are easily obtained by using the proposed method and good performance in the following the desired trajectory is achieved. In summary, the proposed controller is very effective control method for the position control of the excavator's attachment.

• Journal of Advanced Marine Engineering and Technology
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• v.9 no.4
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• pp.339-347
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• 1985

Precise measurement of flowrate is considered as an important measuring factor in mechanical and chemical experiment. Although there are many methods to measure flowrate, Float type flowmeter is widely used because of its easy attachment to piping and high measuring precision. To design the precise float type flowmeter, much design information such as tapered-tube dimensions, float size, float shape and float material is required. In this paper, fundamental design theory for float type flowmeter according to the given flowrate range is suggested and compensation coefficients of volumetric and weight flowrate for water, R-113 and air are calculated.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.279-289
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• 2019

This study is conducted to provide marketing implications for the growing pet-related market by identifying the impact of attachment to pets on the purchasing attributes of pet goods and the relationship between the purchasing attributes, satisfaction, and behavioral intention.The survey was conducted on 173 respondents among the pet owners who had purchased pet products (beauty/bathing goods). The confirmatory factor analysis and path analysis were conducted using SPSS 22.0 and AMOS 21.0. This analysis results showed that attachment to a pet significantly influences the purchasing attributes of pet products: price appropriateness, quality, design, reputation and the sales environment. The relationship between the product purchasing attributes, satisfaction, and behavioral intention showed that price appropriateness, quality, and the sales environment of the product purchasing attributes had a significant impact on satisfaction, but the product's design and reputation do not. Satisfaction has a significant effect on behavioral intention. This study demonstrates that the pet product market should consider product quality, price appropriateness, use and an accessible sales environment based on the characteristics of pets rather than considering the design or reputation of the owner's preference of product.

• The Journal of Korean Academy of Prosthodontics
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• v.54 no.3
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• pp.259-266
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• 2016

In edentulous mandible, implant supported overdenture was considered as a first treatment option. Konus type attachment supplies rigid support and cross arch stabilization so that more favorable force transmission and distribution can be attained. In the dentistry, computer aided design-computer aided manufacturing (CAD-CAM) system makes it possible to fabricate restorations with high precision and effectiveness. Recently, Palladium-silver (Pd-Ag) alloy which is millable has been developed. This article presents that application of CAD-CAM Konus type attachment can be provide satisfactory stability and function on four-implant supported mandibular overdenture.

• Journal of the Korea Fashion and Costume Design Association
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• v.21 no.2
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• pp.27-41
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• 2019

This study aimed to investigate the influence of fashion lifestyles and consumption values on the difficulty of discarding unused fashion products. To achieve this purpose, this study surveyed female consumers aged 20s-50s to gain empirical analysis in July 2018. Data were analyzed for 236 subjects who were selected through online and offline sampling. The results were as follows. First, consumers tend to have difficulties discarding unused fashion goods. Consumers that have a higher income tend to have greater difficulty when choosing to discard items. Factors influencing the difficulty in discarding items were emotional attachment, usefulness in the future, economic value, and the lack of organizing skills. Usefulness in the future and the lack of organizing skills are the most significant factors influencing the discarding difficulty. Second, discarding difficulty had a significant relationship with fashion lifestyle, indicating that consumers seeking a fashion lifestyle that purchase more expensive brands tend to have more difficulty when discarding items. Third, each factor had a meaningful relationship with fashion goods consumption values. Those pursuing conspicuous value showed a greater difficulty discarding items due to emotional attachment, economic value, and lack of organizing skills. Pursuance of situational value showed a higher discarding difficulty than economic value factors. Pursuance of practical value showed a higher difficulty than both usefulness in future situations and economic value factors. Pursuance of differentiation value showed a higher discarding difficulty than emotional attachment and usefulness in the future factor. This study will help understanding consumers psychological mechanisms that experience difficulty in terms of the discarding of unused fashion goods.

• Women's Health Nursing
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• v.22 no.2
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• pp.71-77
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• 2016

Purpose: This study was a comparative study to understand the levels of anxiety, pain and maternal-fetal attachment between women who became pregnant after infertility treatment and became pregnant naturally. Methods: This study used a comparative survey design. Data were collected by 50 couples of natural pregnancy and of who became pregnant after infertility treatment who visited delivery room in C Medical hospital, Seoul. These couples were to have first baby, and cervix dilatation of women was less than 3 cm regardless of diagnosis. Results: The score of anxiety of infertile women was significantly higher than that of naturally pregnant women; however, that of spouses showed no difference. The pain score for infertile women was significantly higher in both the active and transition phases. Pain scores that reported by their spouses did not show differences in either phase. The score of maternal-fetal attachment showed no difference between two groups of women. Conclusion: The result showed the importance of nursing intervention to reduce women's anxiety and pain, through both antenatal-childbirth education programs and assertive nursing interventions. It is necessary to develop and evaluate new intervention which would be more effective for reducing pain and anxiety for couples who became pregnant after infertility treatment.