• Journal of Pharmaceutical Investigation
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• v.3 no.1_2
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• pp.23-33
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• 1973

The effect of atractylis on the renal function of the dog was investigated in this study. Alcohol extract of atractylis, when given intravenously in dose 15mg/kg, elicited antidiuresis rather than diuresis. Also, free water clearance $(C_{H_2O})$ decreased in proportion. No alterations of the renal clearances of creatinine and PAH were detected in the range of doses which are effective in inhibiting diuresis. The effects were obtained with smaller doses when the atractylis was given through the intracarotid artery route rather than intravenously. When infused directly into a renal artery, atratylis exhibited identical action on both kidneys, indicating that the renotropic action is mediated by some endogenous humoral agents. It is therefore suggested that atractylis is capable of releasing ADH from the neurohypophysis.

• The Korean Journal of Pharmacology
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• v.4 no.1 s.5
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• pp.33-36
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• 1968

The effect on schedule controlled behavior and toxicity of Atractylis alkaloid was investigated in the pigeons which were trained on a multiple fixed-ratio fixed-interval schedule of food presentation. Atractylis alkaloid decreased the rate of responding during both the fixed-interval and fixed ratio component of the schedule at 10 mg/kg. Further depression occurred at 30 mg/kg. This 'flat dose-response curve for depression of conditioned behavior was typical of tranquilizers. Conclusively it was suspected that Atractylis alkaloid had major tranquilizing activity.

• YAKHAK HOEJI
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• v.2 no.1_2
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• pp.8-11
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• 1953

Rhizoma atractylis is the root of Atractylis ovata thumb, Form this root, Dexrose (identified as glucosazone), Iso-Vlaeric acid (identified as P-bromophenacyl ester) and Linolic acid (identified as tetrabromide and sativinic acid) are isolated.

• The Plant Pathology Journal
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• v.17 no.4
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• pp.227-230
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• 2001

Bioassay techniques using young leaves and roots were developed to screen resistance of Atractylis spp. against Phytophthora drechsleri. Among 638 plants collected from various regions of Korea from 1994 to 1996, 67 were pre-screened in fields naturally infested with P. drechsleri, which is the causal pathogen of rhizome rot of Atractylis. Among the pre-screened sources, 18 (ca. 26.8%) were highly resistant to the pathogen in leaf inoculation. In the root inoculation test, abundant sporangia were formed in susceptible plant roots, while only a few or no sporangia were produced on the roots which were found resistant in the leaf inoculation test. Among the selected resistant plants, A. japonica 96066 and 96104 were used to cross with another species, A. macrocephala 96362 that showed high yield with good quality of rhizome but susceptible to the pathogen. The F$_1$hybrids designated as HA03 turned out to be resistant to the pathogen, indicating that resistant gene(s) was inherited. Among intra-species hybrids of A. japonica, HA07 and HA09 were resistant to the pathogen in leaf inoculation and moderate in root inoculation. However, HA08 was susceptible in both inoculation tests. This result suggests that the parent material might be genetically heterogeneous. Further genetic study should be carried out to verify this phenomenon.

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.89-98
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• 2003

Pollens of six taxa in the genus Atractytis L. confused with Atractylodes DC. were investigated by the light and the scanning electron microscope. Pollen grains of six taxa in this genus were monads, 46.5-66.7$\mu\textrm{m}$ in length of polar axis, 43.6-60.47$\mu\textrm{m}$ in equatorial width. Aperture was tri-colporate, 19.6-29.1 $\mu\textrm{m}$ in colpus length,7.3-11.0$\mu\textrm{m}$ in colpus width and 6.4-10.6$\mu\textrm{m}$ in porus diameter. The exine was composed of three layers(foot layer, columellne, tectum), and 5.2-8.3 $\mu\textrm{m}$ in thickness. The surface sculpturing pattern was echinate, the spines were 1.4-5.8$\mu\textrm{m}$ in length and 6-33 per 20$\mu\textrm{m}$$^2$ in distribution numbers over the surface. Atractylis L. was not distinguished from Atractylodes DC. by pollen morphology, especially the pollens of Atractylis arabica, A. aristata, A. carduus, A. microcephala were very similar to that of Atractylodes. But its taxonomic significance was different, the taxa of Atractylodes DC. were divided into 2 types by only pollen size, but the treated taxa in this study were divided into various types by pollen size, shape of equatorial view, surface sculpturing and spine size. The characteristics of spine size and shape of equatorial view in A. cancellata and A. prolifera were very useful for consideration of evolutionary trends in this genus.

• Journal of Food Hygiene and Safety
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• v.3 no.2
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• pp.89-97
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• 1988

The possible effeets of some oriental herbs, whleh have been used to treat eaneerllke dllleue In Korea, on growth and afIatonn production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The ertraet of Atractylis ovata and Curcuma longa, also Inhibited the afIatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of O.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycellum weight was 0.953g, and total afatoxin was reduced 5O%($792\mu\textrm{g}$)of that produced in the control($1547\mu\textrm{g}$). Aflatoxin per mycelium weight was decreased 32%($992\mu\textrm{g}$) of that produced in the control($1467\mu\textrm{g}$), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and the aflatoxin production of Aspergillus parasiticus R-716.

• Korean Journal of Pharmacognosy
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• v.5 no.3
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• pp.159-166
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• 1974

The Yellow needle crystal was isolated from Atractylis Rhizoma, having mp $124{\sim}126^{\circ}C$(decomp.), the chemical composition $C_{16}H_{21}N_{3}O_{6}$, and its m.w. 251. The pharmacological actions of this alkaloid were studied by various psycopharmacological experiments. 1) In order to see the effect of this Atractylis(=At.) alkaloid on gross general behaviors in mice, a behavioral analysis experiment was adapted. The occurrence number of sleep and lying in At. alkaloidal animals with the doses 10mg/kg or 20mg/kg was increased but the number of jumping, exploration, rearing and defecation was significantly decreased than those of placebo. 2) The effect of the At. alkaloid on unlearned emotional behaviors of mice was studied with an open-field method. The At. alkaloidal groups with the doses 20mg/kg or 30mg/kg showed less often the frequency of locomotion than that of placebo. 3) To know the effect of the At. alkaloid on the learning, a standard water maze experiment and conditioned avoidance response were conducted. As compared to placebo control, the aquisition rate of the maze learning in the alkaloidal mice with the dose of 10mg/kg or 20mg/kg was significantly impaired and the speed of swimming was also signficantly delayed. In the conditioned avoidance response, the extinction performances of the alkaloidal rats with doses of 20mg/kg or 30mg/kg did not differ significantly than that of placebo.

• YAKHAK HOEJI
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• v.19 no.2
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• pp.65-78
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• 1975

• The Plant Pathology Journal
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• v.18 no.5
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• pp.288-292
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• 2002

Crude extract of Xanthium strumarium inhibited mycelial growth and zoospore germination of Phytophthora drechsleri, the causal agent of Atractylis rot, in vitro. Fresh sap from X. strumarium at 50-fold dilution was highly effective in controlling the disease Incidence in pot and field trials. Purified extracts from cocklebur Inhibited mycelial growth and zoospore germination in vitro at a concentration of 12.5 $\mu\textrm{g}$/ml and 15.6 $\mu\textrm{g}$/ml, respectively. Hyphal tips affected by the compound showed malformation. The antifungal compound puri- fied fromX. strumarium was identified as 4-oxo-1 (5), 2,11, (13)-xanthatriene-12,8-olide, known as "deacetyl xanthumin".min".uot;.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6363-6367
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• 2013

Atractylis lancea (Thunb.) DC. (AL), an important medicinal herb in Asia, has been shown to have anti-tumor effects on cancer cells, but the involved mechanisms are poorly understood. This study focused on potential effects and molecular mechanisms of AL on the proliferation of the Hep-G2 liver cancer cell line in vitro. Cell viability was assessed by MTT test in Hep-G2 cells incubated with an ethanol extract of AL. Then, the effects of AL on apoptosis and cell cycle progression were determined by flow cytometry. Telomeric repeat amplification protocol (TRAP) assays was performed to investigate telomerase activity. The mRNA and protein expression of human telomerase reverse transcriptase (hTERT) and c-myc were determined by real-time RT-PCR and Western blotting. Our results show that AL effectively inhibits proliferation in Hep-G2 cells in a concentrationand time-dependent manner. When Hep-G2 cells were treated with AL after 48h,the $IC_{50}$ was about 72.1 ${\mu}g/mL$. Apoptosis was induced by AL via arresting the cells in the G1 phase. Furthermore, AL effectively reduced telomerase activity through inhibition of mRNA and protein expression of hTERT and c-myc. Hence, these data demonstrate that AL exerts anti-proliferative effects in Hep-G2 cells via down-regulation of the c-myc/hTERT/telomerase pathway.