• Korean Journal of Pharmacognosy
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• v.49 no.1
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• pp.47-54
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• 2018

Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by $^1H$, $^{13}C$ NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. $500{\mu}g/mL$ EAC treatment inhibited Hela cell at $60.2{\pm}1.5%$.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.35-41
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• 2009

Astilbe (Saxifragaceae) is a genus well known for its disjunctive distribution in Asia and eastern North America. In this study, we reconstructed a molecular phylogeny of the genus using the sequences of ITS regions of nuclear ribosomal DNA. A total of 17 species representing major lineage of Astilbe and closely related taxa were included in the phylogenetic analyses. We obtained a Bayesian phylogenetic tree in which Saxifragopsis was positioned as a sister group to Astilbe. The Japanese endemic species, A.platyphylla was the most basal lineage within the genus. This species is well known for its distinct morphological features such as unisexual flowers, apetaly, and calyx with 7-11 lobes. Two species, A. biternata, a New World representative of the genus, and A. rivularis widely distributed in S. Asia, branched off early in the evolution of Astilbe. The remaining species formed a strongly supported core clade, which diverged into two robust geographical lineages: the first ("Japonica" clade) of species distributed in Japan, Taiwan, and Philippines and the other ("Rubra" clade), of taxa in China and Korea. The ITS phylogeny indicates that the Bering land bridges were the major route for the origin and dispersal of A. biternata. The two Taiwanese taxa and A. philippinensis were found to derive from the Japanese member, as the genus advanced southwards. The ITS phylogeny suggests that apetaly originated independently at least two times within the genus. Our results do not support Engler's classification system of the genus based on the leaf type (simple vs. compound), but reaffirm Hara's taxonomic idea which primarily considered the features of calyx.

• Korean Journal of Environment and Ecology
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• v.24 no.4
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• pp.493-499
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• 2010

To obtain the basic data for the conservation and cultivation of major native herb species under the trees, distribution of native herb species, and corrrlations between native herb species and site factors were studied on natural broadleaved forest in Mt. Gariwang and Mt. Jungwang, Pyeunchang-gun. 136 native herb species were distributed in studied area, and the importance percentage of Pseudostellaria heterophylla was proved the highest, 10.8%. The other major herb species were the following oders, Meehania urticifolia, Corydalis remota, Pimpinella brachycarpa, Carex siderosticta, Pseudostellaria palibiniana, Erythronium japonicum, Ligularia fischeri, Asarum heterotropoides var. mandshuricum, Hylomecon vernalis and Astilbe rubra var. rubra. Highly positive correlations were proved Hepatica asiatica and Ainsliaea acerifolia, Pimpinella brachycarpa and Aconitum jaluense subsp. jaluense, Aconitum jaluense subsp. jaluense and Smilacina japonica var. japonica, Meehania urticifolia and Pseudostellaria palibiniana. Seven groups of native herb species were divided by cluster analysis. Preferring site factors for each native herb species were determined. Ligularia fischeri, Ainsliaea acerifolia, Parasenecio auriculata var. matsumurana, Erythronium japonicum and Pimpinella brachycarpa were distributed mainly on the higher sites, Ligularia fischeri were ditributed more frequently in deep soil sites. These results might be useful measures in the conservation and cultivation of native edible and medicinal herb species on the forest floor in the natual broadleaved forest, Pyeungchang-gun.

• Journal of People, Plants, and Environment
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• v.23 no.3
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• pp.255-265
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• 2020

Background and objective: The purpose of this study was to provide guidelines for irrigation management by analyzing the effects of soil moisture on the growth characteristics of herbaceous plants in green infrastructure. Methods: In a rain shelter greenhouse, the growth performance of nine species of experimental plants was assessed under different soil moisture contents (20%, 15%, 10%, 5%, and 1%) for about 5 months to analyze plant growth characteristics due to soil humidity. Methods to determine plant growth conditions include surveying growth conditions of the crowns, stems, leaves, flowers and fruits on the aerial part and surveying growth conditions of the roots in the underground part. Results: The results showed that Mukdenia rossii and Astilbe rubra grew well at 15% moisture content with irrigation intervals of 10 and 13 days, respectively. Soil moisture content of 10% with irrigation intervals of 13 and 17 days was appropriate for Sedum kamtschaticum and Pachysandra terminalis. Similarly, Aquilegia japonica and Liriope platyphylla grew well at 15% moisture content with irrigation intervals of 10 and 17 days. However, Ligularia stenocephala grew well-developed stems and roots at 1% soil moisture content and an irrigation interval of 25 days, while the optimum conditions for Lythrum anceps were 5% moisture content and an irrigation interval of 8 days. Conclusion: Although a limited number of experimental plants were used in this study, this study could propose an appropriate irrigation cycle for planting on artificial soil substrates. Based on these results, it is possible to plan suitable planting designs considered irrigation cycles.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.64-78
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• 2015

The environmental characteristics of Astilboides tabularis were investigated to compile basic data for conservation and restoration. Natural habitats, according to investigations, were mostly located on the slopes of mountains facing north at an altitude of 432 m to 1,413 m above sea level with angles of inclination ranging from 16 degree to 49.5 degree. A total of 246 vascular plant taxa were identified from 12 quadrats in 11 habitats. The importance value of Astilboides tabularis is 20.40%, and 6 highly ranked species such as Dryopteris crassirhizoma (4.56%), Rodgersia podophylla (3.72%), Astilbe rubra (2.81%), Carex siderosticta (2.67%), Ainsliaea acerifolia (2.65%), and Meehania urticifolia (2.46%) are considered to be an affinity with Astilboides tabularis in their habitats. Average species diversity was 1.23, dominance and evenness were found to be 0.11 and 0.86, respectively. The litter depth above the soil surface was 2 to 10 cm, and soil depth was 20.8 to 67.5 cm. The soil texture of habitats were divided three types such as loam, silt loam, and sandy loam. The soil pH was 5.63 to 7.74, and the organic matter and nitrogen contents were ranged from 8.88 to 42.55%, and 1.40 to 6.58 mg/g, respectively.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.497-506
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• 2010

This study intended to investigate the environmental factors including soil and vegetation in order to better understand the environmental and ecological characteristics of ten different habitats of $Hanabusaya$ $asiatica$. These habitats, according to investigations, are mostly located on the slopes of mountains facing north at an altitude of 580 m to 1,396 m above sea level with angles of inclination ranging from 5 degrees to 80 degrees. A total of 146 vascular plant taxa are identified in 32 quadrates of the ten habitats. The importance value of $H.$ $asiatica$ is 8.87%, and 5 highly ranked species such as $Carex$ $siderosticta$ (8.67%), $Ainsliaea$ $acerifolia$ var. $subapoda$ (7.10%), $Calamagrostis$ $arundinacea$ (6.79%), $Athyrium$ $yokoscense$ (5.33%), $Astilbe$ $rubra$ (3.11%) are considered to be an affinity with $H.$ $asiatica$ in their habitats. Dominant species of woody plants in ten habitats are represented as $Quercus$ $mongolica$ in tree layer (T1), $Acer$ $pseudo$-$sieboldianum$ in subtree layer (T2), $Rhododendron$ $schlippenbachii$ and $Tripterygium$ $regelii$ in shrub layer (S). The degree of their average species diversity is 1.30, and that of dominance and evenness are 0.08 and 0.88, respectively. The type of soil is sandy loam, loam and loamy sand, and the average field capacity of soil is 23.95%. Their average organic matter is 12.28%, soil pH 5.79, and available phosphorus is 25.48%. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of tree layers is correlated with richness, diversity, dominance, evenness and coverage of $H.$ $asiatica$.