• Asian-Australasian Journal of Animal Sciences
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• 제25권1호
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• pp.17-21
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• 2012

The purpose of this study was to improve mapping power and resolution for the QTL influencing carcass quality in Hanwoo, which was previously detected on the bovine chromosome (BTA) 6. A sample of 427 steers were chosen, which were the progeny from 45 Korean proven sires in the Hanwoo Improvement Center, Seosan, Korea. The samples were genotyped with the set of 2,535 SNPs on BTA6 that were imbedded in the Illumina bovine 50 k chip. A linkage disequilibrium variance component mapping (LDVCM) method, which exploited both linkage between sires and their steers and population-wide linkage disequilibrium, was applied to detect QTL for four carcass quality traits. Fifteen QTL were detected at 0.1% comparison-wise level, for which five, three, five, and two QTL were associated with carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area (LMA), and marbling score (Marb), respectively. The number of QTL was greater compared with our previous results, in which twelve QTL for carcass quality were detected on the BTA6 in the same population by applying other linkage disequilibrium mapping approaches. One QTL for LMA was detected on the distal region (110,285,672 to 110,633,096 bp) with the most significant evidence for linkage (p< $10^{-5}$). Another QTL that was detected on the proximal region (33,596,515 to 33,897,434 bp) was pleiotrophic, i.e. influencing CWT, BFT, and LMA. Our results suggest that the LDVCM is a good alternative method for QTL fine-mapping in detection and characterization of QTL.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1250-1256
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• 2002

A novel fine-mapping method exploiting linkage disequilibrium (LD) was applied to better refine the quantitative trait loci (QTL) positions for milk production traits on bovine chromosome 14 in the pedigree comprising 22 paternal half-sib families of a Black-and-White Holstein-Friesian grand-daughter design in the Netherlands for a total of 1,034 sons. The chromosome map was constructed with the 31 genetic markers spanning 90 Kosambi cM with the average inter-marker distance of 3.5 cM. The linkage analyses, in which the effects of sire QTL alleles were assumed random and the random factor of the QTL allelic effects was incorporated into the Animal Model, found the QTL for milk, fat, and protein yield and fat and protein % with the Lod scores of 10.9, 2.3, 6.0, 25.4 and 3.2, respectively. The joint analyses including LD information by use of multi-marker haplotypes highly increased the evidence of the QTL (Lod scores were 25.1, 20.9, 11.0, 85.7 and 17.4 for the corresponding traits, respectively). The joint analyses including DGAT markers in the defined haplotypes again increased the QTL evidence and the most likely QTL positions for the five traits coincided with the position of the DGAT gene, supporting the hypothesis of the direct causal involvement of the DGAT gene. This study strongly indicates that the exploitation of LD information will allow additional gains of power and precision in finding and localising QTL of interest in livestock species, on the condition of high marker density around the QTL region.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.309-312
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• 2004

We performed exon trapping in order to locate functional genes on chicken chromosomes (GGA) and to identify functional gene sequences from chicken cosmids. Sequence analysis of 100 clones revealed 17 putative exons, five of which were identified with known sequences in a gene database search: thymopoietin beta (TMPO), U5 snRNP-specific 40 kDa protein (HPRP8BP), dihydropyridine receptor alpha 1 subunit (CACNL1A3), cystein string protein (CPS) and C15orf4. We attempted to map the genes to chicken chromosomes by using FISH and linkage analysis. The chromosomal localizations were GGA1 (TMPO), GGA10 (C15orf4), GGA23 (HPRP8BP) and GGA28 (CPS) by FISH and linkage analysis, while that of CACNL1A3 was predicted to be on a microchromosome by FISH but not by linkage analysis. Comparative mapping analyses between chickens and humans for the genes revealed both known and new synteny. The syntenic conservation between GGA1 and human chromosome (HSA) 12q23 (TMPO) and between GGA10 and HSA15q25 (C15orf4), were consistent with a recent publication, while two new syntenies were observed between GGA28 and HSA20q13.3 in CPS and between GGA23 and HSA1p34-35 in HPRP8BP. The information of presently mapped genes can contribute as anchor markers based on functional genes and the construction of a comparative map.

• 한국보육지원학회지
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• 제10권5호
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• pp.191-209
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• 2014

본 연구는 유아 인성교육에 대한 교사들의 인식을 Kane과 Trochim(2007)의 개념도 방법을 활용하여 밝히고, 그 중요도를 알아보는 데 목적이 있다. 이를 위해 부산광역시에 소재한 유아교육기관에 근무하고 있는 교사 10명을 대상으로 면접을 실시하여 총 196개의 아이디어 진술문이 수집되었으며, 추출된 아이디어 진술문을 통합 편집하는 과정을 거쳐 최종적으로 51개의 진술문으로 축약하였다. 최종 진술문은 다시 10명의 개념도 작성 참여자들에게 분류하도록 하고, 설문조사 참여자 294명에게 각 진술문의 중요도에 관해 평정하는 과정을 거쳤다. 연구결과 첫째, 분류 결과를 토대로 다차원 척도 분석과 위계적 군집분석을 이용하여 2개 차원(환경/개인, 덕목/실행)과 5개 군집으로 이루어진 유아 인성교육에 대한 교사들의 인식 개념도를 도출할 수 있었다. 둘째, 유아 인성교육에 대한 교사들의 중요도 인식을 평정한 결과 유아 인성교육 관련 5개 범주 중 '성인의 역할 강조(M=4.45)'를 가장 중요하게 인식하고 있음을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.941-946
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• 2008

Single-minded 1 gene (SIM1) is a homolog of Drosophila SIM1 gene which plays a key role in the midline cell lineage of the central nervous system and is implicated in the regulation of feeding behavior and obesity in the human and mouse. In this study, porcine SIM1 gene was firstly mapped to chromosome 1p13 using radiation hybrid (RH) mapping and two polymorphisms were detected at position 607 (A/G) in SIM1 intron7 and position 780 (C/T) in SIM1 exon8. The last substitution was genotyped in a 364 F2 animal-population and an association analysis of these genotypes was performed with growth, carcass and meat quality traits by the statistical animal model. The results showed the significant influence of the SIM1 genotype on growth (p<0.05): live weight at birth, later period of growth and average daily gain; and effects on carcass composition (p<0.05): weight of head and buck kneed foreleg, backfat depth, loin eye area, carcass leaf fat and ham fat weights; and traits related to intramuscular fat content (p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제25권12호
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• pp.1649-1659
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• 2012

Crosses between Korean and Landrace pigs have revealed a large quantitative trait loci (QTL) region for fat deposition in a region (89 cM) of porcine chromosome 4 (SSC4). To more finely map this QTL region and identify candidate genes for this trait, comparative mapping of pig and human chromosomes was performed in the present study. A region in the human genome that corresponds to the porcine QTL region was identified in HSA1q21. Furthermore, the LMNA gene, which is tightly associated with fat augmentation in humans, was localized to this region. Radiation hybrid (RH) mapping using a Sus scrofa RH panel localized LMNA to a region of 90.3 cM in the porcine genome, distinct from microsatellite marker S0214 (87.3 cM). Two-point analysis showed that LMNA was linked to S0214, SW1996, and S0073 on SSC4 with logarithm (base 10) of odds scores of 20.98, 17.78, and 16.73, respectively. To clone the porcine LMNA gene and to delineate the genomic structure and sequences, including the 3'untranslated region (UTR), rapid amplification of cDNA ends was performed. The coding sequence of porcine LMNA consisted of 1,719 bp, flanked by a 5'UTR and a 3'UTR. Two synonymous single nucleotide polymorphisms (SNPs) were identified in exons 3 and 7. Association tests showed that the SNP located in exon 3 (A193A) was significantly associated with weight at 30 wks (p<0.01) and crude fat content (p<0.05). This association suggests that SNPs located in LMNA could be used for marker-assisted selection in pigs.

• 한국지역지리학회지
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• 제11권5호
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• pp.474-487
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• 2005

본 연구 목적은 2001년에 시행된 미국 NAEP 지리 평가에 대한 학생들의 문항반응경향성을 분석하여 귀납적으로 성취수준을 기술하는데 있다. NAEP 지리 평가는 4 8 12학년 학생을 대상으로 실시되는데, 평가영역은 내용영역과 행동영역으로 구분된다. 내용영역은 공간과 장소 환경과 사회 공간직 역동성과 연계성이고, 행동영역은 지식 이해 적용이다. 성취수준은 기초 숙달 수월수준으로 구분된다. 본 연구에서는 각 수준과 대표 문항을 연계하는 방법(item mapping method)을 사용하여 성취수준에 대하여 분석하였다. 그 결과 NAEP 지리 성취수준 설정위원회에서 제시한 성취수준과 대표 문항 분석에 기초한 성취수준은 큰 차이가 있는 것으로 나타났다. 대표 문항 분석을 기초로 도출된 성취수준은 실제 학생들의 문항반응에 기초하여 도출되었기 때문에 지리교육과정 개선을 위한 효과적인 송환 역할을 할 수 있을 것이다.

• 한국지리정보학회지
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• 제11권1호
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• pp.80-89
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• 2008

위성영상을 이용한 지도제작 분야는 입체영상 획득이 가능한 SPOT 위성이 발사되면서 이에 대한 정량적인 분석이 가능해졌다. 특히, 고해상도 위성영상은 항공사진촬영이 불가능하여 대축척 지도제작이 곤란한 지역 또는 지상기준점 측량이 불가능한 지역에 대한 수치지도 제작 분야에 있어서 효율적인 방법으로 주목을 받고 있다. 이에 본 연구에서는 미국 국가지형공간정보국(NGA;The National Geospatial-Intelligence Agency)에서 제공하는 정사영상 자료를 이용하여 지역적 특성을 고려한 지형정보를 추출하고 이에 대한 평면위치 정확도를 분석하였다. 이를 위해 일정한 크기로 타일링된 정사영상에 대해 국토지리정보원에서 제공하는 축척 1:5000 수치지도를 기준으로 지역적 특성에 따른 정확도를 평가하였고 이를 바탕으로 비접근 지역의 지형정보 획득을 위한 기 구축 정사영상의 활용 가능성을 제시하고자 하였으며 비접근 및 난접근 지역에서의 지상기준점 획득에 대한 제한을 극복하는 수단으로 본 연구의 결과를 활용하고자 하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권9호
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• pp.1327-1333
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• 2007

Lactose synthase catalyses the formation of lactose which is the major osmole of bovine milk and regulates the milk volume. Alpha-lactalbumin (${\alpha}$-LA) is involved in the synthesis of lactose synthase in the mammary gland. Therefore ${\alpha}$-LA is regarded as a plausible candidate gene for the milk yield trait. To determine whether ${\alpha}$-LA is associated with milk performance traits, 1,028 Chinese Holstein cows were used to detect polymorphisms in the ${\alpha}$-LA by means of single-strand conformation polymorphism (SSCP). Two nucleotide transitions were identified in the 5'flanking region and intron 3 of ${\alpha}$-LA. Associations of such polymorphisms with five milk performance traits were analyzed using a general linear model procedure. No significant associations were observed between these polymorphisms and the five milk performance traits (p>0.05). RH mapping placed ${\alpha}$-LA on BTA5q21, linked most closely to markers U63110, CC537786 and L10347 (LOD>8.3), which is far distant from the region of the quantitative trait locus (QTL) on bovine chromosome 5 for variation in the milk yield trait. In summary, based on our findings, we eliminated these SNPs from having an effect on milk performance traits.

• Asian-Australasian Journal of Animal Sciences
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• 제28권7호
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• pp.926-935
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• 2015

The efficiency of genome-wide association analysis (GWAS) depends on power of detection for quantitative trait loci (QTL) and precision for QTL mapping. In this study, three different strategies for GWAS were applied to detect QTL for carcass quality traits in the Korean cattle, Hanwoo; a linkage disequilibrium single locus regression method (LDRM), a combined linkage and linkage disequilibrium analysis (LDLA) and a $BayesC{\pi}$ approach. The phenotypes of 486 steers were collected for weaning weight (WWT), yearling weight (YWT), carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area, and marbling score (Marb). Also the genotype data for the steers and their sires were scored with the Illumina bovine 50K single nucleotide polymorphism (SNP) chips. For the two former GWAS methods, threshold values were set at false discovery rate <0.01 on a chromosome-wide level, while a cut-off threshold value was set in the latter model, such that the top five windows, each of which comprised 10 adjacent SNPs, were chosen with significant variation for the phenotype. Four major additive QTL from these three methods had high concordance found in 64.1 to 64.9Mb for Bos taurus autosome (BTA) 7 for WWT, 24.3 to 25.4Mb for BTA14 for CWT, 0.5 to 1.5Mb for BTA6 for BFT and 26.3 to 33.4Mb for BTA29 for BFT. Several candidate genes (i.e. glutamate receptor, ionotropic, ampa 1 [GRIA1], family with sequence similarity 110, member B [FAM110B], and thymocyte selection-associated high mobility group box [TOX]) may be identified close to these QTL. Our result suggests that the use of different linkage disequilibrium mapping approaches can provide more reliable chromosome regions to further pinpoint DNA makers or causative genes in these regions.