• Journal of Korean Academy of Nursing
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• v.27 no.4
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• pp.975-987
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• 1997

The purpose of this study was to investigate the current status of curriculum operation of the basic medical sciences in nursing education at college of nursing, department of nursing and junior college of nursing, ultimately to provide the basic data to improve a curriculum of basic medical science in nursing education. 78 professors who were in charge of basic medical science at 22 colleges of nursing and department of nursing, and 20 junior colleges of nursing responded the questionnaire consisted of 22 question items about the status of objectives, lectures, laboratory practice and characteristics of professors, and mailed to the author. The findings of this study were as follows : 1. The subjects of basic medical science were identified as physiology, anatomy, biochemistry, pathology, microbiology, pharmacology in the most colleges of nursing and junior colleges of nursing. 2 colleges of nursing and department of nursing(9.1%) and 19 junior colleges of nursing(95%) did not open biochemistry, 1 college of nursing and department of nursing(5%) did not open pathology and pharmacology. 2 Junior colleges of nursing(10%) did not open pharmacology, 1 junior college of nursing(5%) did not open pathology, the other 1 junior college of nursing did not open microbiology. 2. Credits of the subjects were ranged from 1 to 4. Lecture hours of one semester of physiology at school of nursing and junior college of nursing was average 103.6 and average 102.67, that of anatomy was average 127.1 and average 98, that of microbiology was average 109.7 and average 86.33, that of biochemistry was average 105, that of pathology was average 91 and average 94, that of pharmacology was average 86 and average 85.75. 3. Most of schools used 1 textbook for lectures, 3 school of nursing and department of nursing recommended references without using textbook, while all 36 junior colleges of nursing used textbooks. 4. 5 among 10 schools of nursing and department of nursing had a laboratory practice in physiology, 4 among 7 schools in anatomy, 4 among 6 schools in biochemistry, 2 among 6 schools in pathology 5 among 6 schools in microbiology. Not all the schools had a laboratory practice in pharmacology. 4 among 9 junior colleges of nursing had a laboratory practice in physiology. 1 among 4 schools in anatomy, 2 among 7 schools in microbiology. Not all the junior colleges of nursing had a laboratory practice in pathology and pharmacology. 11 among 20 colleges of nursing and department of nursing, 4 among 7 junior schools of nursing used a textbook of laboratory practice. 5. All the subjects at school of nursing and department of nursing responded that content of lectures and laboratory practices of basic medical science should be different from that of medical education, 34 junior schools of nursing responded that content of lecture of basic medical science in nursing education should be different from that of medical education. 33 junior schools of nursing responded that content of practice of basic medical science in nursing education should be different from that of medical education. 6. The final degree of 25 professors who were in charge of basic medical science were doctors of. medicine, that of 5 professors were masters of medicine, that of 5 were doctor of pharmacology, that of 2 were a master of pharmacology, that of 1 was physical science. The final degree of 8 professors who were in charge of basic medical science were masters of medicine, 7 doctors of medicine, 4 masters of nursing science, 4 masters of pharmacology, 2 doctors of nursing, 2 doctors of physical science, 2 doctors of pharmacology and 1 master of public health. 9 full professors, 13 associate professors, 11 assist ant professors, 3 full time instructors, and 6 part time instructors were in charge of basic medical science at college of nursing and department of nursing, 20 part time instructors, 8 associate professors, 6 assistant professors, and 2 full professors were in charge of has basic medical science at junior college of nursing. Based on these results, curriculum of basic medical science in nursing education should be reviewed deeply based on nursing model.

• Reproductive and Developmental Biology
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• v.33 no.2
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• pp.71-76
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• 2009

Salivary lipocalin (SAL1) is a member of the lipocalin protein family that has a property to associate with many lipophilic molecules. The importance of SAL1 during pregnancy in pigs has been suggested by our previous study which has shown that SAL1 is expressed in the uterine endometrium in a cell type- and implantation stage-specific manner and secreted into the uterine lumen. However, function of SAL1 in the uterus during pregnancy in pigs is not known. To understand SAL1 function in the uterus during pregnancy, we generated recombinant porcine SAL1 protein in an insect cell line. Porcine SAL1 cDNA was cloned into a baculovirus expression vector using RT-PCR and total RNA from uterine endometrium on day 12 of pregnancy, and the expression vector was used to generate recombinant Bacmid containing the SAL1 gene. The recombinant Bacmid was then transfected Sf9 cell to produce recombinant baculovirus. By infecting Sf9 cell with recombinant baculovirus, we established a SAL1-expressing insect cell expression system. Immunoblot analysis confirmed SAL1 expression in the infected cells. Recombinant SAL1 produced by the Sf9 cell line will be useful for understanding physiological function of SAL1 during pregnancy in pigs.

• Animal Bioscience
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• v.36 no.9
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• pp.1357-1366
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• 2023

Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes and to explore their effects on inosine-5'-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line). Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of DUSP8 and IGF2 genes, respectively. One SNP (rs313443014 C>T) in DUSP8 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens. Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The IGF2 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed. Conclusion: The SNPs in the DUSP8 and IGF2 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.

• BMB Reports
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• v.36 no.3
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• pp.299-304
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• 2003

For deciphering the cyclic guanosine monophosphate (cGMP) signaling pathway, we employed chemical proteomics to identify the novel target molecules of cGMP. We used cGMP that was immobilized onto agarose beads with linkers directed at three different positions of cGMP. We performed a pull-down assay using the beads as baits on tissue lysates and identified 9 proteins by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry. Some of the identified proteins were previously known cGMP targets, including cGMP-dependent protein kinase and cGMP-stimulated phosphodiesterase. Surprisingly, some of the co-precipitated proteins were never formerly reported to associate with the cGMP signaling pathway. The competition binding assays showed that the interactions are not by nonspecific binding to either the linker or bead itself, but by specific binding to cGMP. Furthermore, we observed that the interactions are highly specific to cGMP against other nucleotides, such as cyclic adenosine monophosphate (cAMP) and 5'-GMP, which are structurally similar to cGMP. As one of the identified targets, MAPK1 was confirmed by immunoblotting with an anti-MAPK1 antibody. For further proof, we observed that the membrane-permeable cGMP (8-bromo cyclic GMP) stimulated mitogen-activated protein kinase 1 signaling in the treated cells. Our present study suggests that chemical proteomics can be a very useful and powerful technique for identifying the target proteins of small bioactive molecules.

• Journal of Service Research and Studies
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• v.8 no.3
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• pp.37-49
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• 2018

In this study, we conducted an exploratory study for designing service science as a degree program that will lead the 21st century. In the new era, the convergence of industries is accelerating and the mobility of the profession is greatly increased, so the talents to be trained in higher education will be greatly changed. We must cultivate human resources capable of creating their own jobs through competency development and cultivate talented individuals who can demonstrate creative initiative in the ecosystem where the boundaries between industries are dismantled. To this end, it is required to switch from the existing vertical majors and systems to horizontal majors, and service majors are expected to play a key role in this transition. In this study, we designed the service science degree program reflecting the changes of the new economy and reflecting the demand of the new education. The service science curriculum provides an analysis as it can award a degree as an independent major, or may serve as a sub-major or an associate major with existing majors. Service studies are expected to play the most effective role in cultivating the capabilities defined by the 21st century human resources in the World Economic Forum.

• Fisheries and Aquatic Sciences
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• v.21 no.6
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• pp.15.1-15.9
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• 2018

Background: Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity was evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Results: Ozonated krill oil significantly inhibited nitric oxide (NO) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Ozonated krill oil also reduced the mRNA expression of inflammatory cytokines such as interleukin (IL)-$1{\beta}$, IL-6, and tumor necrosis factor (TNF)-${\alpha}$ in LPS-stimulated RAW 264.7 macrophages. To elucidate the mechanism underlying the anti-inflammatory activity of ozonated krill oil, we evaluated the effects of ozonated krill oil on the activation of mitogen-activated protein kinases (MAPKs) pathway. Ozonated krill oil suppressed the LPS-stimulated phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). Conclusion: This study revealed that the ozonated krill oil exhibited an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages. To the best of our knowledge, this is the first report that ozonated krill oil suppressed pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 macrophages by inhibiting the phosphorylation of p38 MAPK and JNK.

• Journal of Korean Library and Information Science Society
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• v.50 no.2
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• pp.191-214
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• 2019

In order to understand the trends of collaborative research in the field of Library and Information Science (LIS) in Korea, this study analyzed bibliometric data and keywords of 5,383 Journal papers by 195 Korean LIS professors from 2000 to 2017 as well as the author credit allocation formulas of 26 Korean university research evaluation criteria. Examination of university research evaluation criteria revealed co-authors' credit level to be generally much lower than that of single authors, which in turn reduces the relative value of collaborative research. As a result, recent journals publish more co-authored papers than single author papers both domestically and internationally. The study also found collaborative research to be less prevalent in private universities than national universities and least prevalent in associate professors among professors. Furthermore, keyword analysis of study data revealed the emerging topics of both domestic and international collaborative research to be those that reflect social phenomena as well as those that relate to information science employing new technologies.

• Journal of Mechanical Science and Technology
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• v.16 no.12
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• pp.1583-1593
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• 2002

This paper discusses the model validation and vibration suppression of an AMB flexible rotor via additional LQG controller. The main difficulty in the vibration suppression of the flexible rotor using AMB is to realize a controller that can minimize resonance without injuring the stabilized rigid modes. In order to solve this problem, simple scheme for system modeling and controller design are developed. Firstly, the AMB flexible rotor is stabilized with a PID controller, which leads to a new stable rotor-bearing system. Then, authors propose the model validation procedure using measured open-loop frequency responses to obtain an accurate model of the AMB flexible rotor system. After that, LQG controller with modal weighting is designed to suppress resonances of the stable rotor-bearing system. Due to the poor controllability and observability of flexible modes compared to rigid ones, balancing of two Gramians is prerequisite for the fair LQG controller design. Simulation with step disturbance and experimental results of unbalance response up to 10,000 rpm verified the effectiveness of the proposed scheme.

• Korean Journal of Applied Biomechanics
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• v.28 no.1
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• pp.61-67
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• 2018

Objective: The human body is often modelled as a spring-mass system. Lower extremity stiffness has been considered to be one of key factor in the performance enhancement of running, jumping, and hopping involved sports activities. There are several different classification of lower extremity stiffness consisting of vertical stiffness, leg stiffness, joint stiffness, as well as muscle and tendon stiffness. The primary purpose of this paper was to review the literature and describe different stiffness models and discuss applications of stiffness models while engaging in sports activities. In addition, this paper provided a current update of the lower extremity literature as it investigates the relationships between lower extremity stiffness and both functional performance and injury. Summary: Because various methods for measuring lower extremity stiffness are existing, measurements should always be accompanied by a detailed description including type of stiffness, testing method and calculation method. Moreover, investigator should be cautious when comparing lower extremity stiffness from different methods. Some evidence highlights that optimal degree of lower extremity stiffness is required for successful athletic performance. However, the actual magnitude of stiffness required to optimize performance is relatively unexplored. Direct relationship between lower extremity stiffness and lower extremity injuries has not clearly been established yet. Overall, high stiffness is potentially associate risk factors of lower extremity injuries although some of the evidence is controversial. Prospective injures studies are necessary to confirm this relationship. Moreover, further biomechanical and physiological investigation is needed to identify the optimal regulation of the lower limb stiffness behavior and its impact on athletic performance and lower limb injuries.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.1
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• pp.881-889
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• 2021

The purpose of this study is to descriptively reveal the demographic and business profile and personal-entrepreneurial characteristics in Manado, the capital of North Sulawesi, and secondly to associate these profiles and characters to their business progress. A sample size of 21 respondents was drawn - selected from those who warmly welcomed the interviewers for an open-ended structured questionnaire. SPSS 24 has been employed to descriptively reveal the sample distribution according to demographic factors and business entities and to determine the dominant factors affecting the progress of the business by testing the hypothesis on the association of variables under study using specified statistical analytical tools, such as regression analysis, especially stepwise regression formula, between specified dependent variables and independent variables and /or between all variables. The stepwise regression analysis has enabled the researcher to determine which variables are the most important reflecting the personal characteristics theorized as "locus of control": self-efficacy, needs for achievement, personal traits, and barriers to business progress The analysis reveals that the progress of business does have an association and is dependent on the source of capital and education, needs for achievement and locus of control.