• 방사성폐기물학회지
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• 제15권4호
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• pp.403-409
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• 2017

원자력발전소내 습식저장중인 사용후핵연료의 건식저장을 위해서는 캐니스터 내부에 사용후핵연료를 옮겨 담은 이후, 건식저장 캐니스터 내장품이나 사용후핵연료 다발의 부식방지 및 피복관의 열화방지를 위해 모든 수분은 제거해야 한다. 캐니스터 내부의 수분을 제거하는데 사용할 수 있는 기체강제순환 건조시스템 개발을 위한 연구개발이 진행중이다. 본 연구에서는 기체강제순환 건조시스템 설계, 제작을 위한 예비설계를 수행하였다. 예비설계에는 캐니스터 내부 잔존수분 제거를 위한 건조사례조사, 건조관련 규격이나 표준, 건조합격기준, 건조장치구성, 현장요구분석, 습식저장중인 사용후핵연료 특성을 포함하였다. 예비설계를 통하여 기체강제순환 건조시스템의 설계 개념도와 P&ID를 도출하였고, 이를 활용하여 건조시스템 제작을 위한 상세설계를 수행할 것이다.

• 산업경영시스템학회지
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• 제43권2호
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• pp.72-78
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• 2020

Demand for cosmetics with functionality and eco-friendliness has increased dramatically due to recent aging, well-being trends, and increased interest in beauty. Cosmetics production in 2014 was 8,970.4 billion won, an increase of about 50% compared to 6,014.6 billion won in 2010. In the midst of this, similar companies in intense competition are pursuing differentiated strategies and innovation activities to solve quality, price and delivery problems. In particular, cosmetics packaging work is getting more difficult due to the increasing bill of materials (BOM) and difficult assembly methods. Therefore, in this study, the following problems were identified and suggestions for the improvement of the packaging Many research laboratories such as biotechnology, chemistry, and pharmaceuticals, which are undergoing various studies, are equipped with ready-made laboratory safety equipments such as bio-safety workbenches, aseptic bases, and exhaust workbenches. However, most researchers are disadvantaged in using existing safety equipment. This is because existing safety equipment can not take into account all of the unique characteristics of the research. For this reason, researchers are demanding the development of customized safety equipment that is well suited to their research needs. process of Company C, which is facing difficult situation to respond to the customer 's delivery due to the 52 - hour work week. First, we used the stopwatch to find the difficulty process in the packaging process and show ways to improve it. Second, to improve the efficiency of line balancing in the packaging process, we integrate processes, improve work methods, and perform simple automation. As a result, the prepare loss for replacement was reduced by 1 minute from 5 minutes, resulting in a 23% increase in productivity from 112 ea./hour to 137ea./ hour per person. At this time, the LOB of the packaging process was improved from 70% to 82% by operating one more production line through one person per line, total 9 people saving.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2002년도 학술발표대회
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.141-141
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• 2017

The ubiquitin-proteasome pathway is the major regulatory mechanism in a number of cellular processes for selective degradation of proteins and involves three steps: (1) ATP dependent activation of ubiquitin by E1 enzyme, (2) transfer of activated ubiquitin to E2 and (3) transfer of ubiquitin to the protein to be degraded by E3 complex. F-box proteins are subunit of SCF complex and involved in specificity for a target substrate to be degraded. F-box proteins regulate many important biological processes such as embryogenesis, floral development, plant growth and development, biotic and abiotic stress, hormonal responses and senescence. However, little is known about the F-box genes in wheat. The draft genome sequence of wheat (IWGSC Reference Sequence v1.0 assembly) used to analysis a genome-wide survey of the F-box gene family in wheat. The Hidden Markov Model (HMM) profiles of F-box (PF00646), F-box-like (PF12937), F-box-like 2 (PF13013), FBA (PF04300), FBA_1 (PF07734), FBA_2 (PF07735), FBA_3 (PF08268) and FBD (PF08387) domains were downloaded from Pfam database were searched against IWGSC Reference Sequence v1.0 assembly. RNA-seq paired-end libraries from different stages of wheat, such as stages of seedling, tillering, booting, day after flowering (DAF) 1, DAF 10, DAF 20, and DAF 30 were conducted and sequenced by Illumina HiSeq2000 for expression analysis of F-box protein genes. Basic analysis including Hisat, HTseq, DEseq, gene ontology analysis and KEGG mapping were conducted for differentially expressed gene analysis and their annotation mappings of DEGs from various stages. About 950 F-box domain proteins identified by Pfam were mapped to wheat reference genome sequence by blastX (e-value < 0.05). Among them, more than 140 putative F-box protein genes were selected by fold changes cut-offs of > 2, significance p-value < 0.01, and FDR<0.01. Expression profiling of selected F-box protein genes were shown by heatmap analysis, and average linkage and squared Euclidean distance of putative 144 F-box protein genes by expression patterns were calculated for clustering analysis. This work may provide valuable and basic information for further investigation of protein degradation mechanism by ubiquitin proteasome system using F-box proteins during wheat development stages.

• 한국산업보건학회지
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• 제26권1호
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• pp.58-63
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• 2016

Objectives: One major limitation encountered in retrospective exposure assessment for epidemiological study is the lack of exposure records and information maintained by companies which if they existed would allow the estimation of past exposure to hazardous operations and agents. This study developed a job-specific questionnaire(JSQ) to estimate exposure profiles among semiconductor workers, including operation and job. Methods: This JSQ can be directly applied to workers who work or have worked in a wafer fabrication or a chip packaging and assembly facility. Results and Conclusions: We used this JSQ to obtain past exposure information from semiconductor workers via face-to-face investigation. Major contents include questions on the facilities, operations and jobs to which they have been exposed since they entered employment in the semiconductor industry. The total number of questions in the JSQ is 18. Responses to this JSQ can be used not only to estimate retrospective exposure to operations and jobs in the semiconductor industry, but also to associate with the risk of all causes of death and risk of disease, including cancer.

• 한국구조물진단유지관리공학회 논문집
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• 제22권4호
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• pp.1-9
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• 2018

일반적인 플랜트 산업에서의 파이프라인은 지지형식으로만 구조설계를 하고 있으며 시공방법이 용접에 의한 단순접합방법을 선호하고 있다. 그러나 플랜트 산업 특성상 용접 불꽃에 의한 화재발생시 매우 위험함으로 통상적인 고장력 볼트 접합을 단순화하여 내진성능이 확보되는 파이프랙 접합방식을 개선하는 연구이다. 연구결과 H-Beam 대 H-Beam의 접합기술의 향상과, 내진성능이 향상된 파이프랙 구조기술 개발, 파이프랙 제작 및 설치 시간 단축 기술 개발, 다양한 방법의 철 구조물 설치 기술 개발, 적용분야 확대에 따른 기술의 접근성 향상이라는 효과를 얻을 수 있다. 경제적 측면에서는 기존 파이프랙 공법에 비해 현장설치 공정 축소로 인건비 및 장비비 절감 효과를 기대할 수 있으며, 자재비의 경우 H-Beam 연결을 위한 고장력 볼트의 수량 감소효과 대비 대량생산을 위한 금형비용 발생으로 원가가 절감될 수 있다. 안정성 측면에는 기존의 현장설치에서 나타났던 다소 큰 위험성 요소를 최소화시킬 수 있는 공장제작 공정을 통해 접합 작업자의 안정성을 증대시킬 수 있을 것으로 판단된다.

• 대한영양사협회학술지
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• 제10권2호
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• pp.205-217
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• 2004

This study was carried out to provide information on the effect of nutrition education program for diabetic patients at the Guri City's Public Health Center. Subjects of this study were 31 persons(male 7, female 24) who attended all courses of "2002 Diabetes Education Class". They were indicated as the 'education group'(EG). Eating and living habits of EG were investigated before the education. EG's weight and blood glucose (post prandial 2 hours, PP2) were examined as well. EG's PP2 reduction was compared with a 'control group'(CG) who didn't join any course in that class. All of the subjects were non-insulin-dependent diabetes mellitus(NIDDM) patients. EG's average age was 62.4$\pm$8.8. Before taking the course, EG's PP2 was 251.5$\pm$29.6mg/dl, and body mass index(BMI) was 26.3$\pm$2.3 on average. Most of them were stressed out from their daily lives and usually had no exercise. Most people of EG ate meals rapidly and liked sweet and fatty foods. After the course of training, EG's weight and BMI before the training were not decreased significantly. However, all of the EG's PP2s, which were measured 4 times(before the meal at the special lunch session, after 2 hours at this meal, after 2 weeks and 4 weeks dietary assembly), were decreased in comparison with the PP2 which was checked prior to joining the training. EG's average PP2 was more reduced than CG's one. In addition, all groups' PP2s were decreased for 8 weeks. After all, this nutritional education at the public health center was effective in glycemic control for diabetes mellitus patients. Especially, when the dietary assembly as practical training was included in the educational process, the patient's dietary intake and PP2 was improved more effectively. Therefore, this study suggests that nutrition work at public health centers is necessary for the Health Promotion Policy.

• 한국건축시공학회지
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• 제8권6호
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• pp.139-145
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• 2008

The objective of this paper is to analyze the construction management capacity of Choseon Dynasty and suggest a new interpretation on the construction capacity of the time management by reviewing the time management case during a representative construction project, HwaSungk construction project implemented in the latter period of Choseon Dynasty around 200 years ago. which was described in HwaSungSungYoukEuGye. The analysis results of the time management capacity during the latter period of Choseon Dynasty depicted in the HwaSungSungYoukEuGye is summarized as follows: (1) It took 2 years and 6 months to complete the Hwasung construction project. However, all of the single buildings were constructed within 6 months, a relatively short period of time. Judging from the fact, it can be assumed that the construction techniques using wood of the time were very sophisticated. (2) When the HwaSung was constructed, it took a relatively short period of time to complete works from placing the foundation to erecting columns and to placing a crossbeam on the columns. Based on the fact, it can be also inferred that assembly processing techniques of the time were also sophisticated and the level of member processing and assembling techniques of the time was considerably good as well. (3) The HwaSung construction was continued throughout the year without any influence by weather conditions, which tells us that division of labor by work was performed, and it was possible to mobilize labor force for the construction project even during the busy farming seasons.

• 식물병연구
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• 제23권3호
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• pp.207-211
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• 2017

2016년 12월에 산림보호법 중 일부가 개정되어 이른바 '나무의사법'이 공포되었으며, 이 법은 2018년 6월 28일부터 시행될 예정이다. 새로운 법안에서는 나무의사 자격 소지자에 한하여 나무병원을 개업할 수 있고, 나무병원만이 생활권 공공분야의 수목병해충 관리를 할 수 있도록 규정하고 있으며, 그에 따라 '나무의사'라는 국가공인자격이 신설되었다. 나무의사가 되기 위해서는 지정된 양성과정을 이수하고 시험을 통과하여야 한다. 현재 산림청에서는 이 법의 시행을 위하여 구체적 시행방안을 포함하는 시행령과 시행규칙 등을 만들고 있다. 수목진료 및 건강관리에서 가장 핵심적인 부분이 식물병리학이라는 사실을 감안할 때, 그리고 식물병리학을 공부하는 학생들의 사회진출을 위하여 식물병리학회는 새로운 수목진료체계가 정착될 수 있도록 나무의사 양성기관의 교육과정 개발 및 나무의사 선발시험계획 수립 등에 적극적으로 관여하여야 할 것으로 생각한다.

• Tribology and Lubricants
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• 제23권3호
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• pp.130-133
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• 2007

Fuel cladding tubes in nuclear fuel assembly are held up by supporting grids because the tubes are long and slender. Fluid flows of high-pressure and high-temperature in the tubes cause oscillating motions between tubes and supports. This is called as FIV (flow induced vibration), which causes fretting wear in contact parts of tube and support. The fretting wear of tube and support can threaten the safety of nuclear power plant. Therefore, a research about the fretting wear characteristics of tube-support is required. The fretting wear tests were performed with supporting grids and cladding tubes, especially after corrosion treatment on tubes, in water. The tests were done using various applied loads with fixed amplitude. From the results of fretting tests, the wear amounts of tube materials can be predictable by obtaining the wear coefficient using the work rate model. Due to stick phenomena the wear depth was changed as increasing load and temperature. The maximum wear depth was decreased as increasing the water temperatures. At high temperatures there are the regions of some severe adhesion due to stick phenomena.