• Title/Summary/Keyword: Assembly Structure

Search Result 817, Processing Time 0.026 seconds

State of the art on the physical mapping of the Y-chromosome in the Bovidae and comparison with other species - A review

  • Rossetti, Cristina;Genualdo, Viviana;Incarnato, Domenico;Mottola, Filomena;Perucatti, Angela;Pauciullo, Alfredo
    • Animal Bioscience
    • /
    • v.35 no.9
    • /
    • pp.1289-1302
    • /
    • 2022
  • The next generation sequencing has significantly contributed to clarify the genome structure of many species of zootechnical interest. However, to date, some portions of the genome, especially those linked to a heterogametic nature such as the Y chromosome, are difficult to assemble and many gaps are still present. It is well known that the fluorescence in situ hybridization (FISH) is an excellent tool for identifying genes unequivocably mapped on chromosomes. Therefore, FISH can contribute to the localization of unplaced genome sequences, as well as to correct assembly errors generated by comparative bioinformatics. To this end, it is necessary to have starting points; therefore, in this study, we reviewed the physically mapped genes on the Y chromosome of cattle, buffalo, sheep, goats, pigs, horses and alpacas. A total of 208 loci were currently mapped by FISH. 89 were located in the male-specific region of the Y chromosome (MSY) and 119 were identified in the pseudoautosomal region (PAR). The loci reported in MSY and PAR were respectively: 18 and 25 in Bos taurus, 5 and 7 in Bubalus bubalis, 5 and 24 in Ovis aries, 5 and 19 in Capra hircus, 10 and 16 in Sus scrofa, 46 and 18 in Equus caballus. While in Vicugna pacos only 10 loci are reported in the PAR region. The correct knowledge and assembly of all genome sequences, including those of genes mapped on the Y chromosome, will help to elucidate their biological processes, as well as to discover and exploit potentially epistasis effects useful for selection breeding programs.

Difference analysis of the collapse behaviors of the single-story beam-column assembly and multi-story planar frame

  • Zheng Tan;Wei-Hui Zhong;Bao Meng;Xing-You Yao;Yu-Hui Zheng;Yao Gao;Shi-Chao Duan
    • Steel and Composite Structures
    • /
    • v.50 no.3
    • /
    • pp.265-280
    • /
    • 2024
  • The collapse behavior observed in single-story beam-column assembly (SSBCA) do not accurately represent the actual overall stress characteristic of multi-story frame structure (MSFS) under column loss scenario owing to ignoring the interaction action among different stories, leading to a disconnection between the anti-collapse behaviors of "components" and "overall structures", that is, the anti-collapse performance of frame structures with two different structural scales has not yet formed a combined force. This paper conducts a numerical and theoretical study to explore the difference of the collapse behaviors of the SSBCA and MSFS, and further to reveal the internal force relationships and boundary constraints at beam ends of models SSBCA and MSFS. Based on the previous experimental tests, the corresponding refined numerical simulation models were established and verified, and comparative analysis on the resistant-collapse performance was carried out, based on the validated modeling methods with considering the actual boundary constraints, and the results illustrates that the collapse behaviors of the SSBCA and MSFS is not a simple multiple relationship. Through numerical simulation and theoretical analysis, the development laws of internal force in each story beam under different boundary constraints was clarified, and the coupling relationship between the bending moment at the most unfavorable section and axial force in the composite beam of different stories of multi story frames with weld cover-plated flange connections was obtained. In addition, considering the effect of the yield performance of adjacent columns on the anti-collapse bearing capacities of the SSBCA and MSFS during the large deformation stages, the calculation formula for the equivalent axial stiffness at the beam ends of each story were provided.

The C-terminal Region of Human Tau Protein with Ability of Filament Formation

  • Chung, Sang-Ho
    • Animal cells and systems
    • /
    • v.1 no.2
    • /
    • pp.317-321
    • /
    • 1997
  • Tau protein is one of the microtubule-associated proteins in the mammalian brain. In Alzheimer's disease, tau protein is immobilized in the somatodendritic compartment of certain nerve cells, where it forms a part of the paired helical filament (PHF). To understand the role of tau protein in the formation of PHF, a recombinant human tau protein expressed in Escherichia coli and five synthetic peptide fragments (peptide 1 to peptide 5), corresponding to the C-terminal region of tau protein, were prepared and their ability in self-assembly to form filamentous structures was examined. The recombinant human tau protein formed short rod-like structures in 0.1M MES buffer containing 1 mM $MgCI_2$, while a synthetic peptide fragment 1 containing 55 amino acid residues could assemble into a lot of long filamentous structures in water and particularly twisted helical structures in 0.1M MES buffer containing 1 mM $MgCI_2$. This suggests that the C-terminal region possesses a filament-forming ability and may be related to the formation of the helical structure by providing a powerful filament-forming driving force.

  • PDF

Self-Aggregation of Synthetic Magnesium Bacteriochlorins as a Photosynthetic Antenna Model

  • Kunieda, Michio;Mizoguchi, Tadashi;Tamiaki, Hitoshi
    • Journal of Photoscience
    • /
    • v.9 no.2
    • /
    • pp.353-355
    • /
    • 2002
  • We prepared 3-(1-hydroxyethyl)-bacteriopyrochlorophy11-a (3) possessing magnesium atom and phytyl ester from modification of natural bacteriochlorophyll(BChl)-a. A dichloromethane solution of (3$^1$R) and (3$^1$S)-3 was diluted with 100~1000 fold volume of cyclohexane to give new species absorbing near-infrared lights. The resulting Q, maximum of (3$^1$R)-3 was 860 nm and red-shifted by 2150 $cm^{-1}$ / from the monomeric. In the nonpolar organic solvent, epimeric (3$^1$S)-3 showed a 1ess red-shifted peak at 798 nm as well as a residual monomeric band. Such visible spectra indicated that 3 diastereose1ectively aggregated in cyclohexane to afford oligomers possessing a simi1ar supramolecular structure with chlorosomal aggregates of natural BChl-d, 7,8-dehydro-form of 3.

  • PDF

Synthesis of Silver Nanocrystallites by a New Thermal Decomposition Method and Their Characterization

  • Lee, Don-Keun;Kang, Young-Soo
    • ETRI Journal
    • /
    • v.26 no.3
    • /
    • pp.252-256
    • /
    • 2004
  • We formed silver nanocrystallites by the thermal decomposition of a $Ag^{+1}$-oleate complex, which was prepared by a reaction with $AgNO_{3}$ and sodium oleate in a water solution. The resulting monodispersed silver nanocrystallites were produced by controlling the temperature (290$^{\circ}$C). Transmission electron microscopic (TEM) images of the particles showed a 2-dimensional assembly of the particles with a diameter of $9.5{\pm}0.7nm$, demonstrating the uniformity of these nanocrystallites. An energy-dispersive X-ray (EDX) spectrum and X-ray diffraction (XRD) peaks of the nanocrystallites showed the highly crystalline nature of the silver structure. We analyzed the decomposition of the $Ag^{+1}$-oleate complex using a Thermo Gravimetric Analyzer (TGA) and observed the crystallization process using XRD.

  • PDF

Fabrication of Nano-bridge Using a Suspended Multi-Wall Carbon Nanotube (다중벽 탄소나노튜브를 이용한 나노 브리지 제작)

  • Lee, Jong-Hong;Won, Moon-Cheol;Seo, Hee-Won;Song, Jin-Woo;Han, Chang-Soo
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.24 no.3 s.192
    • /
    • pp.134-139
    • /
    • 2007
  • We report the suspension of individual multi-walled carbon nanotubes (MWNTs) from the bottom substrate using deep trench electrodes that were fabricated using optical lithography. During drying of the solution in dielectrophoretic assembly, the capillary force pulls the MWNT toward the bottom substrate, and it then remains as a deformed structure adhering to the bottom substrate after the solution has dried out. Small-diameter MWNTs cannot be suspended using thin electrodes with large gaps, but large-diameter MWNTs can be suspended using thicker electrodes. We present the statistical experimental results for successful suspension, as well as the feasible conditions for a MWNT suspension based on a theoretical approach.

Performance Evaluations of a Novel Prototype of High Frequency Non-Contact Power Transformer

  • Gamage, Laknath;Ishitobi, Manabu;Nakaoka, Mutsuo
    • Journal of Power Electronics
    • /
    • v.4 no.1
    • /
    • pp.12-17
    • /
    • 2004
  • In this paper, a practical implementation to reduce leakage flux of a high-frequency inverter based non-contact type power transformer composed of EE-shape ferrite cores is presented for key technology of the next generation medical use X-ray CT scanner system. Design consideration for the unique structure of the non-contact power transformer with 900mm in diameter is also introduced. The complete non-contact transformer is actually arranged by several blocks of the magnetic circuit assembled by using 10 small EE shape cores with 120mm in length. It is experimentally and analytically discussed from a reduced leakage flux viewpoint related to its inductively coupling coefficient. A practical method to lower the leakage flux is described based on effective Copper-Sheet- Treatment placed on EE shape ferrite cores of magnetic circuit.

Experimental Study for Construction Equipment's Cabin Noise Control (건설장비 캐빈 저소음화를 위한 시험적 연구)

  • Lee, Tae-Kyoung;Joo, Won-Ho;Bae, Jong-Gug
    • Transactions of the Korean Society for Noise and Vibration Engineering
    • /
    • v.19 no.8
    • /
    • pp.802-808
    • /
    • 2009
  • In this paper, the experimental study on the identification of noise sources and noise transmission paths was carried out for the cabin noise control of construction equipment. In order to investigate noise and vibration characteristics of cabin structure, sound absorption, transmission, and radiation tests were performed using cabin assembly models. The noise/vibration source levels were obtained from the real cabins of wheel loader and excavator. Using transfer functions of cabins and real cabins' source data, cabin noise was decomposed into airborne and structureborne noise transmissions. Finally noise sources and major transmission paths were successfully identified for wheel loader and excavator's cabins.

Supramolecular Micelle from Amphiphilic Mn(III)-porphyrin Derivatives as a Potential MRI Contrast Agent

  • Choi, Kwang-Mo;Lee, Do-Hyung;Jang, Woo-Dong
    • Bulletin of the Korean Chemical Society
    • /
    • v.31 no.3
    • /
    • pp.639-644
    • /
    • 2010
  • Amphiphilic porphyrin derivatives have been synthesized and characterized by $^1H$ NMR and MALDI-TOF-MS. All porphyrin derivatives showed very high solubility to aqueous medium as well as hydrophobic organic solvent. The UV-vis absorption of the porphyrin derivatives showed significant broadness and decrease of maximum intensity of absorption in aqueous solution. SEM experiment showed the formation of spherical micellar structure. The $T_1$ relaxation time of aqueous medium was drastically decreased in the presence of Mn(III)-porphyrin derivative, indicating that the supramolecular micelle has strong possibility to use as a $T_1$ contrast agent.

Developing an Introductory Training Course to PLM (PLM 입문을 위한 교육과정 개발)

  • Do, Namchul
    • Korean Journal of Computational Design and Engineering
    • /
    • v.18 no.1
    • /
    • pp.28-35
    • /
    • 2013
  • Product Life cycle Management (PLM) is an indispensable tool for manufactures to develop competitive products in an efficient way. This enlarges the number of participants for product development who should understand PLM for their supporting activities. However, burdens for developing example products, maintaining complex PLM systems and training prerequisite skills for engineering tools such as CAD systems prohibit an efficient introductory course to PLM. This paper proposes a comprehensive introductory course to PLM that bases on general product development process. In addition, it enables participants to build their example products with familiar Lego blocks and to construct 3D CAD assembly models by using predefined 3D elements. The PLM system for the course provides an intuitive and simple user environment for participants to specify their parts lists, associated 3D CAD models, and product structure of example products. Experiences on a class of the course show it is a valid and efficient education and training method for the PLM introduction.