• 반도체디스플레이기술학회지
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• 제22권1호
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• pp.72-77
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• 2023

In many manufacturing settings, including the semiconductor industry, products are completed by producing and assembling various components. Sorting out from randomly mixed parts and classification operations takes a lot of time and labor. Recently, many efforts have been made to select and assemble correct parts from mixed parts using robots. Automating the sorting and classification of randomly mixed components is difficult since various objects and the positions and attitudes of robots and cameras in 3D space need to be known. Previously, only objects in specific positions were grasped by robots or people sorting items directly. To enable robots to pick up random objects in 3D space, bin picking technology is required. To realize bin picking technology, it is essential to understand the coordinate system information between the robot, the grasping target object, and the camera. Calibration work to understand the coordinate system information between them is necessary to grasp the object recognized by the camera. It is difficult to restore the depth value of 2D images when 3D restoration is performed, which is necessary for bin picking technology. In this paper, we propose to use depth information of RGB-D camera for Z value in rotation and movement conversion used in calibration. Proceed with camera calibration for accurate coordinate system conversion of objects in 2D images, and proceed with calibration of robot and camera. We proved the effectiveness of the proposed method through accuracy evaluations for camera calibration and calibration between robots and cameras.

• 공업화학
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• 제34권3호
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• pp.247-251
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• 2023

유기용매 상에서 레시틴은 양친매성 분자의 특성 때문에 구형의 역 마이셀로 자가조립된다. 이러한 레시틴 용액에 D-sorbitol, 물과 같은 첨가제가 들어갈 경우 레시틴의 분자 형태의 변화를 유도하여 역 실린더형 마이셀로의 변환을 이끌게 된다. 이번 연구에서는, 레시틴과 D-sorbitol/물의 혼합물을 이용하여 샘플의 유변학적 변화를 관찰한다. 또한, 이러한 유변학적 변화와 용액 내부의 자가조립된 나노구조체와의 연관성을 확인하기 위해 엑스선 소각 산란분석기 (SAXS)를 이용하여 나노구조체의 형태 및 크기 등을 확인한다. 이러한 혼합물을 이용하여 제조된 점도가 높고 점탄성을 지닌 유체는 약물전달, 식품젤 등의 분야에 활용이 가능할 것으로 기대된다.

• 한국컴퓨터정보학회논문지
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• 제14권10호
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• pp.101-110
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• 2009

6LoWPAN은 IEEE 802.15.4 MAC과 PHY 계층 위에서 IPv6 패킷을 전송하기 위해 IPv6 헤더 압축, TCP/UDP/ICMP 헤더 압축, 단편화 및 재조립 등을 수행하는 적응계층이다. 그러나 보안 관점에서 보면 기존 IP 계층에서 단편화 및 재조립으로 인한 보안 취약점을 그대로 가지고 있으며 6LoWPAN 적응계층 고유의 새로운 보안 취약점에 노출될 수 있다. 센서 노드는 특성상 재생공격으로 인해 단편화된 패킷의 재전송이 빈번하게 발생하게 되면 심각한 통신장애가 발생한다. 본 논문에서는 6LoWPAN 계층에서 패킷 단편화로 발생할 수 있는 보안 취약점을 분석하고 재생공격으로 인한 재전송을 최소화할 수 있는 메커니즘을 제안하였다. 6LoWPAN 표준을 기반으로 추가적인 재전송 절차 및 단편화 패킷 구조를 설계하고 재전송 지연시간을 분석하였다. 제안하는 메커니즘은 타임스템프, 난스, 체크섬을 도입하여 센서 노드에 가해질 수 있는 재생공격을 최소화한다. 이를 통해 불필요한 패킷 단편화 및 재조립을 제거함으로써 센서 노드의 재조합 버퍼 오버플로우, 통신 속도 저하, 컴퓨팅 자원 손실, 노드 재부팅 등을 최소화시켜 통신의 신뢰도를 높일 수 있다.

• Animal Bioscience
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• 제35권9호
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• pp.1289-1302
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• 2022

The next generation sequencing has significantly contributed to clarify the genome structure of many species of zootechnical interest. However, to date, some portions of the genome, especially those linked to a heterogametic nature such as the Y chromosome, are difficult to assemble and many gaps are still present. It is well known that the fluorescence in situ hybridization (FISH) is an excellent tool for identifying genes unequivocably mapped on chromosomes. Therefore, FISH can contribute to the localization of unplaced genome sequences, as well as to correct assembly errors generated by comparative bioinformatics. To this end, it is necessary to have starting points; therefore, in this study, we reviewed the physically mapped genes on the Y chromosome of cattle, buffalo, sheep, goats, pigs, horses and alpacas. A total of 208 loci were currently mapped by FISH. 89 were located in the male-specific region of the Y chromosome (MSY) and 119 were identified in the pseudoautosomal region (PAR). The loci reported in MSY and PAR were respectively: 18 and 25 in Bos taurus, 5 and 7 in Bubalus bubalis, 5 and 24 in Ovis aries, 5 and 19 in Capra hircus, 10 and 16 in Sus scrofa, 46 and 18 in Equus caballus. While in Vicugna pacos only 10 loci are reported in the PAR region. The correct knowledge and assembly of all genome sequences, including those of genes mapped on the Y chromosome, will help to elucidate their biological processes, as well as to discover and exploit potentially epistasis effects useful for selection breeding programs.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.28-28
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• 2022

This paper presents the first genome assemblies of Philippine mangoes that provide valuable reference for varietal improvement and genomic studies on mango and related fruit crops. WE sequenced whole genomes of3 species, Mangifera odorata (Huani), Mangifera altissima (Paho), and Mangifera indica 'Carabao' (Sweet Elena). 'Carabao' is the major export variety of the Philippines; Paho is identified as vulnerable by the IUCN Red List of Threatened Species; Huani has fruit sap acrid which is the primary defense mechanism against insects and birds. We used Falcon, a diploid aware -de novo assembler to assemble SMRT generated long-read sequences. Falcon-unzip was employed to phase the output assembly producing larger contig sets (primary contigs) and shorter contigs corresponding to haplotypes (haplotigs). Assembly statistics were generated by comparing the assembly to a reference genome, Tommy Atkins, using Quality Assessment Tool (QUAST). Moreover, the extent of duplication and completeness of gene content was measured using Benchmarking Universal Single-Copy Orthologs (BUSCO). Draft assemblies with high duplications were processed using Purge Haplotigs and Purge Dups to lessen duplications with minimal impact on genome completeness. De novo assemblies of Huani, Paho and 'Carabao' were then generated with primary contig sizes of 463.64 Mb, 508.95 Mb and 401.51 Mb respectively. These draft assemblies of Huani, Paho and 'Carabao' showed 96.90%, 95.17% and 99.07% complete BUSCOs respectively which is comparable to 'Tommy Atkins' genome (98.6%). Using two mango transcriptome data (pooled RNA-seq from different mango varieties and tissues), 91-96% or 24-30 million reads were successfully mapped back for each generated assembly indicating high degree of completeness. The results obtained demonstrated the highly contiguous, phased, and near complete genome assembly of three Philippine mango species for structural and functional annotation of gene units, especially those with economic importance.

• IMMUNE NETWORK
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• 제23권3호
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• pp.23.1-23.17
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• 2023

Inflammation is a series of host defense processes in response to microbial infection and tissue injury. Inflammatory processes frequently cause extracellular acidification in the inflamed region through increased glycolysis and lactate secretion. Therefore, the immune cells infiltrating the inflamed region encounter an acidic microenvironment. Extracellular acidosis can modulate the innate immune response of macrophages; however, its role for inflammasome signaling still remains elusive. In the present study, we demonstrated that macrophages exposed to an acidic microenvironment exhibited enhanced caspase-1 processing and IL-1β secretion compared with those under physiological pH. Moreover, exposure to an acidic pH increased the ability of macrophages to assemble the NLR family pyrin domain containing 3 (NLRP3) inflammasome in response to an NLRP3 agonist. This acidosis-mediated augmentation of NLRP3 inflammasome activation occurred in bone marrow-derived macrophages but not in bone marrow-derived neutrophils. Notably, exposure to an acidic environment caused a reduction in the intracellular pH of macrophages but not neutrophils. Concordantly, macrophages, but not neutrophils, exhibited NLRP3 agonist-mediated translocation of chloride intracellular channel protein 1 (CLIC1) into their plasma membranes under an acidic microenvironment. Collectively, our results demonstrate that extracellular acidosis during inflammation can increase the sensitivity of NLRP3 inflammasome formation and activation in a CLIC1-dependent manner. Thus, CLIC1 may be a potential therapeutic target for NLRP3 inflammasome-mediated pathological conditions.

• Molecules and Cells
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• 제47권1호
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• pp.100001.1-100001.8
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• 2024

In eukaryotes, a primary protein quality control (PQC) process involves the destruction of conformationally misfolded proteins through the ubiquitin-proteasome system. Because approximately one-third of eukaryotic proteomes fold and assemble within the endoplasmic reticulum (ER) before being sent to their destinations, the ER plays a crucial role in PQC. The specific functions and biochemical roles of several E3 ubiquitin ligases involved in ER-associated degradation in mammals, on the other hand, are mainly unknown. We identified 2 E3 ligases, ubiquitin protein ligase E3 component N-recognin 1 (UBR1) and ubiquitin protein ligase E3 component N-recognin 2 (UBR2), which are the key N-recognins in the N-degron pathway and participate in the ER stress response in mammalian cells by modulating their stability. Cells lacking UBR1 and UBR2 are hypersensitive to ER stress-induced apoptosis. Under normal circumstances, these proteins are polyubiquitinated through Lys48-specific linkages and are then degraded by the 26S proteasome. In contrast, when cells are subjected to ER stress, UBR1 and UBR2 exhibit greater stability, potentially as a cellular adaptive response to stressful conditions. Although the precise mechanisms underlying these findings require further investigation, our findings show that cytoplasmic UBR1 and UBR2 have anti-ER stress activities and contribute to global PQC in mammals. These data also reveal an additional level of complexity within the mammalian ER-associated degradation system, implicating potential involvement of the N-degron pathway.

• 한국광학회지
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• 제35권4호
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• pp.170-174
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• 2024

대기 중 레이저 빔 전파 시 대기 난류에 의한 악영향을 해소하고 빔품질을 향상시키기 위해서 적응광학을 사용한다. 본 논문에서는 장거리 레이저 전송을 위한 적응광학계의 핵심 부품인 변형거울의 정밀 조립 방법을 제안한다. 변형거울은 거울판과 구동기, 플렉셔(flexure)로 구성되며 이 부품들을 정밀하게 조립하는 것이 성능을 좌우한다. 본 논문에서는 지그의 설계/제작을 통해 고밀집 다채널 실리콘-카바이드 변형거울을 완성했으며, 변형거울 성능 확인을 통해 제안한 방법의 유효성을 검증했다.

• Bulletin of the Korean Chemical Society
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• 제33권5호
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• pp.1581-1585
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• 2012

Two pyridinecarboxamide dicyanidecobalt(III) building blocks and two mononuclear seven-coordinated macrocycle manganese(II) compounds have been rationally selected to assemble cyanide-bridged heterobimetallic complexes, resulting in three cyanide-bridged $Co^{III}-Mn^{II}$ complexes. Single X-ray diffraction analysis show that these complexes $\{[Mn(L^1)][Co(bpb)]\}ClO_4{\cdot}CH_3OH{\cdot}0.5H_2O$ ($\mathbf{1}$), $\{[Mn(L^2)][Co(bpb)]\}ClO_4{\cdot}0.5CH_3OH$ ($\mathbf{2}$) and ${[Mn(L^1)][Co(bpb)]\}ClO_4{\cdot}H_2O$ ($\mathbf{3}$) ($L^1$ = 3,6-diazaoctane-1,8-diamine, $L^2$ = 3,6-dioxaoctano-1,8-diamine; $bpb2^{2-}$ = 1,2-bis(pyridine-2-carboxamido)benzenate, $bpmb2^{2-}$ = 1,2-bis(pyridine-2-carboxamido)-4-methyl-benzenate) all present predictable one-dimensional single chain structures. The molecular structures of these one-dimensional complexes consists of alternating units of $[Mn(L)]^{2+}$ ($L=L^1$ or $L^2$) and $[Co(L^{\prime})(CN)2]^-$ ($L^{\prime}=bpb2^{2-}$, or $bpmb2^{2-}$), forming a cyanide-bridged cationic polymeric chain with free $ClO_4{^-}$ as the balance anion. The coordination geometry of manganese(II) ion in the three one-dimensional complexes is a slightly distorted pentagonal-bipyrimidal with two cyanide nitrogen atoms at the trans positions and $N_5$ or $N_3O_2$ coordinating mode at the equatorial plane from ligand $L^1$ or $L^2$. Investigation over magnetic properties of these complexes reveals that the very weak magnetic coupling between neighboring Mn(II) ions connected by the diamagnetic dicyanidecobalt(III) building block. A best-fit to the magnetic susceptibility of complex ${\mathbf}{1}$ leads to the magnetic coupling constants $J=-0.084(3)cm^{-1}$.

• 한국통신학회논문지
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• 제37권2B호
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• pp.130-137
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• 2012

본 논문은 백본 망에서 발생하는 에너지를 절감하기 위하여 LPI(Low Power Idle)를 이용하는 OBS 코어 및 에지 라우터 구조를 제안하고 이를 평가한다. 제안한 LPI를 이용하는 코어 라우터는 코어 라우터 라인 카드와 BCP 스위치, 버스트 스위치, 스위치 제어기 및 LPI 기능을 위한 Sleep/Wake 제어기로 구성된다. 망 부하가 낮을 때 네트워크 제어 패킷을 수신한 Sleep/Wake 제어기는 코어 라우터 라인 카드의 수면/활성 상태를 제어함으로써 에너지를 절감 할 수 있다. 에지 라우터는 액세스 라인 카드 스위치, SCU 그리고 OBS 에지 라우터 라인 카드로 구성된다. 에지 라우터에서 LPI 기능은 네트워크 수준 제어를 통하여 에지 라우터 라인 카드 개별적으로 수행되며 버스트를 생성하는 동안 에지 라우터 라인 카드의 PHY/Transceiver를 수면 상태로 천이시킴으로서 에너지 절감을 할 수 있다. 제안된 코어 및 에지 라우터 구조의 에너지 절감 성능 평가를 위하여 본 논문에서는 제안된 라우터 구조의 전력 소모율을 비교/분석하였으며, OPNET을 이용한 시뮬레이션을 수행하여 코어 라우터와 에지 라우터 라인 카드의 PHY/Transceiver의 수면시간 관점에서 코어 및 에지 라우터의 에너지 절감 성능을 평가하였다.