• Mycobiology
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• 제49권6호
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• pp.559-581
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• 2021

The coastal marine ecosystems of Vietnam are one of the global biodiversity hotspots, but the biodiversity of marine fungi is not well known. To fill this major gap of knowledge, we assessed the genetic diversity (ITS sequence) of 75 fungal strains isolated from 11 surface coastal marine and deeper waters in Nha Trang Bay and Van Phong Bay using a culture-dependent approach and 5 OTUs (Operational Taxonomic Units) of fungi in three representative sampling sites using next-generation sequencing. The results from both approaches shared similar fungal taxonomy to the most abundant phylum (Ascomycota), genera (Candida and Aspergillus) and species (Candida blankii) but were different at less common taxa. Culturable fungal strains in this study belong to 3 phyla, 5 subdivisions, 7 classes, 12 orders, 17 families, 22 genera and at least 40 species, of which 29 species have been identified and several species are likely novel. Among identified species, 12 and 28 are new records in global and Vietnamese marine areas, respectively. The analysis of enzyme activity and the checklist of trophic mode and guild assignment provided valuable additional biological information and suggested the ecological function of planktonic fungi in the marine food web. This is the largest dataset of marine fungal biodiversity on morphology, phylogeny and enzyme activity in the tropical coastal ecosystems of Vietnam and Southeast Asia. Biogeographic aspects, ecological factors and human impact may structure mycoplankton communities in such aquatic habitats.

• Journal of Species Research
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• 제12권3호
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• pp.197-202
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• 2023

The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

• Mycobiology
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• 제50권6호
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• pp.399-407
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• 2022

Endophytic fungi occupy inner plant tissues, which results in various interactions between the fungus and host. Studies on endophytic fungi have been conducted in Korea for over 30 years. This paper summarizes the published results of those studies. The endophytic fungi of approximately 132 plant species in Korea have been studied since the 1990s, resulting in over 118 publications. The host plants featured in these studies comprised 3 species of mosses, 34 species of woody plants, and 95 species of herbaceous plants. At the family level, the most studied plants were members of the Poaceae family, covering 18 species. Regionally, these studies were conducted throughout Korea, but over half of the studies were conducted in Gyeongsangbuk-do, Gangwon-do, and Chungcheongnam-do. Relatively few studies have been conducted in a metropolis such as Seoul. We confirmed 5 phyla, 16 classes, 49 orders, 135 families, 305 genera, and 855 taxa of endophytic fungi, excluding Incertae sedis, whose relationship with others are unknown. Most of the endophytic fungi belonged to Ascomycota (93.2%), and a few belonged to Basidiomycota (3.6%). Since the diversity of endophytic fungi differs depending on the host plant, plant tissue, and distribution region, future studies should be conducted on multiple host plants and in various regions. Future studies on endophytic fungi are expected to broaden, including genomics and taxonomic and ecological studies of secondary metabolites.

• Mycobiology
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• 제50권6호
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• pp.429-438
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• 2022

Two fungal strains belonging to Ascomycota were discovered in Gyeonggi-do, Korea, during this investigation of soil microfungi and microbiota of insects. The strain KNUF-20-047 produced milky white on the back and a milky creamy center to white toward the margin on the front side of colonies. Conversely, the closest Xenoacremonium falcatus displayed a pale luteous to luteous center, white toward margins on the front side, and pale luteous or luteous pigment on the back side, whereas X. recifei produced white colonies. The conidiophores of KNUF-20-047 were slightly larger than those of X. falcatus, and the conidia were distinct from X. recifei. Strain KNUF-20-NI-005 produced light brown to subhyaline conidiophores up to 56.0 lm tall, whereas Rhinocladiella anceps displayed golden to dark brown conidiophores up to 350 lm. Strain KNUF-20-NI-005 also produced larger conidia than R. anceps but smaller than R. coryli and R. fasciculata. Moreover, the molecular phylogeny strongly supports the detailed description and illustration of each proposed species to be designated as Xenoacremonium minutisporum sp. nov. and Rhinocladiella terrigenum sp. nov. in Korea.

• Mycobiology
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• 제51권4호
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• pp.216-229
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• 2023

Acarosporaceae is a crustose lichen and is known as a species that has more than 50 multispores, and has hyaline spores. Those taxa are often found in rock and soil in mountain areas or coastal regions in Korea, and very diverse forms and species are known. However, after an overall genetic phylogenetic analysis of carbonized ascomata in 2015, species consisting only of the morphological base are newly divided, and several species of Acarosporaceae in Korea are also being discovered in this situation. As a result of analysis using internal transcribed spacer (ITS) and nuLSU gene analysis, Korean species belonged to Acarospora and Sarcogyne clade, and Acarospora classified as the Acarospora clade was mixed with the Polysporina group and the Sarcogyne clade is mixed with the Acarospora. We identified two new species (Acarospora beangnokdamensis J. S. Park & S. O. Oh, sp. nov., Sarcogyne jejuensis J. S. Park & S. O. Oh, sp. nov.) through morphological, molecular, and secondary metabolite substance and found one new record (Sarcogyne oceanica K. Knudsen & Kocourk). We have made a classification key for Acarospora and Sarcogyne in Korea and reported all information together here.

• 한국균학회지
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• 제51권4호
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• pp.491-504
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• 2023

To explore fungal diversity in orchard soil where fire-blighted apple trees are buried, we collected soil samples from apple orchards in Chungju, Korea. Fungal isolates were obtained from DG18 agar and identified at the species level based on morphological features and phylogenetic analyses. The colony characteristics and microstructures were examined using a light microscope and a scanning electron microscope after culturing on potato dextrose agar (PDA), malt extract agar (MEA), Czapek yeast agar (CYA), and oatmeal agar (OA) The PCR-amplified products of the ITS1-5.8S-ITS2 region and 28S large subunit of the nuclear ribosomal RNA gene, as well as partial sequences of the β-tubulin, calmodulin, and translation elongation factor 1-α genes were sequenced and analyzed phylogenetically. Seven previously unknown fungal species were explored in Korea. All samples, including Aspergillus aureolatus, Botryotrichum atrogriseum, Dactylonectria novozelandica, Fusarium denticulatum, Paecilomyces tabacinus, Sarcopodium tibetense and Talaromyces stollii, had ascomycetes. Herein, we report their descriptions and features.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.19-19
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• 2015

The term of Mushrooms means to spread like the April showers. After 18th century, the mycelium and spores of mushrooms were observed by microscope and then was denominated as fungi. About one hundred thousand species of mushrooms in appearance were worldly reported, and in Korea about four thousand species of mushrooms are estimated. In Korea, total of one thousand nine hundred one species of mushrooms have been recorded. Mushrooms belonging to the group of organisms called fungi, which must obtain their food from living plants or animals or from their remains after death. A large number of mushrooms grow in association with the roots of trees and other woody plants, called mycorrhizal fungi, both mushrooms and plants require this relationship for growth and development. And also many Mushrooms are saprobic, living on decayed various fallen leaves, twigs, trees and vegetable remains and etc. some of these million of spores settles on the proper habitat, these spores germinates and grows into a mass of threads, then a mycelium. This is the vegetable part of the mushrooms, what we call mushrooms are the carpophores, all the characteristics of the morphological features are appropriately used to identify species of mushrooms. Recently, identification and classification of mushrooms are newly confirmed by molecular analysis. In 2013, One thousand nine hundred one species of mushrooms in "List of Mushrooms in Korea" which published by the Korean Society of Mycology were recorded. Total of 238species, 107genera, 40families, 13orders, 6Classes belong to phylum Ascomycota. Total of 1,663species, 403genera 81families, 18orders, 7classes belong to phylum Basidiomycota.

• 한국균학회지
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• 제42권1호
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• pp.79-85
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• 2014

태안반도에 자생하는 해안식물인 해국(Aster sphathulifolius Maxim.), 갯개미취(Aster tripolium L.), 갈대(Phragmites australis), 갯꾸러미풀(Puccinellia nipponica Ohwi) 의 뿌리로부터 총 42주의 내생진균이 분리되었다. 그리고 분리된 내생진균의 ITS지역의 염기서열을 이용하여 동정한 결과 모두 자낭균문에 속하며, 8종류의 목(Botryosphaeriales, Capnodiales, Diaporthales, Dothideales, Eurotiales, Helotiales, Hypocreales, and Pleosporales)과 13종류의 속(Alternaria, Aureobasidium, Cadophora, Cladosporium, Davidiella, Diaporthe, Fusarium, Gibberella, Macrophomina, Metarhizium, Neosartorya, Penicillium, and Phoma)으로 분류되었으며, Eurotiales목의 Penicillium속의 분포비율이 가장 높게 존재하였다. Shannon 다양성지수를 비교한 결과 4종의 해안식물 중 갯개미취에서 분리한 내생진균의 다양성이 가장 높게 확인되었다.

• Mycobiology
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• 제45권1호
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• pp.1-8
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• 2017

Despite the huge worldwide diversity of Trichoderma (Hypocreaceae, Ascomycota), only about 22 species have been reported in Korea. Thus, between 2013 and 2015, soil-derived Trichoderma spp. were isolated to reveal the diversity of Korean Trichoderma. Phylogenetic analysis of translation elongation factor 1 alpha gene was used for identification. Among the soil-derived Trichoderma, Trichoderma albolutescens, T. asperelloides, T. orientale, T. spirale, and T. tomentosum have not been previously reported in Korea. Thus, we report the five Trichoderma species as new in Korea with morphological descriptions and images.

• Mycobiology
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• 제46권2호
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• pp.101-113
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• 2018

Lichen genus Stereocaulon (Schreb.) Hoffm is distributed throughout the world. Although 15 Stereocaulon species have been recorded in Korea, no detailed taxonomic or revisionary research has been conducted for nearly two decades. In this study, we collected 260 putative Stereocaulon spp. samples and identified the species based on morphological, chemical, and molecular characteristics. From the collected samples, 10 species of Stereocaulon were identified, nine of which had already been reported, although this was the first report for the tenth, S. octomerellum Hue, in Korea. General characteristics of Stereocaulon spp. include coralloid phyllocladia and tubercular cephalodia; however, the specimen first collected in Korea was a rare species with tomentum on the pseudopodetia. The specimen of S. octomerellum is characterized by the presence of a primary thallus, granule to short coralloid phyllocladia, and pseudopodetia up to 1 cm in size, with tubercular cephalodia. To determine the phylogeny of the specimens, we compared the ITS sequences of ribosomal DNA and the b-tubulin gene sequences. Phylogenetic analysis showed that the Korean Stereocaulon species were monophyletic and placed in the previous phylogenetic classification. Species of S. intermedium and S. exutum, however, were polyphyletic, and are morphologically variable and widespread species. Overall, we present here detailed morphological and chemical descriptions of each species identified and a revised key of all known Stereocaulon species in South Korea.