• Title/Summary/Keyword: Artificial seedling

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Production of 8-ketotrichothecenes by Fusarium graminearum on Corn and Barley (옥수수와 보리에서 Fusarium graminearum의 8-ketotrichothecenes 생성)

  • 서영수;서정아;손황배;이인원
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.418-424
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    • 1998
  • The production of 8-ketotrichothecenes, deoxynivalenol (DON), nivalenol (NIV), and their monoacetyl derivatives was studied in rice and corn cultures using 8 isolates of Fusarium graminearum which were obtained from corn and barley samples. Higher concentrations of trichothecenes were produced on rice than corn, and production of the toxins on rice was enhanced by growing the fungi at $25^{\circ}C$. The isolates were used for evaluation of toxin production and pathogenicity after artificial inoculation to 5 corn and 3 barley cultivars. The kinds and the relative amounts of trichothecenes produced in cultures were consistent with those in infected kernels of corn and barley with some exceptions. As for DON chemotypes, the ratios of 15-acetyl-DON to 3-acetyl-DON were varied among the pathogen-cultivar interactions. The corn and barley cultivars showed the significant differences of resistance to the Fusarium isolates in disease severity and seedling blight, and resistance ranking to the different isolates was varied. However, significant correlations were observed between the total concentrations of trichothecenes in infected kernels of corn and barley and pathogenicities of the Fusarium isolates to the hosts.

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A Study on Resistant Comparisons of Landscape Trees by Simulated Acid Rain (인공산성우에 대한 조경수목의 내성 비교)

  • 정용문
    • Journal of Korean Society for Atmospheric Environment
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    • v.7 no.3
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    • pp.208-218
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    • 1991
  • To compare the resistance of landscape trees by acid rain, simulated acid rain was treated on the seeds of 6 coniferous species (Pinus densiflora, Pinus thunbergii, Pinus koraiensis, Taxus cuspidata, Cedrus deodara, Thuja occidentalis) and 6 broad-leafed species (Ligustrum obtusifolium, Acer Buergerianum, Acer Ginnala, Styrax obassia, Cornus Kousa, Magnolia obovata). The experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agri-Tech Junior College. The seeds of 12 tree species were planted on the nursery soil in the early spring of 1991. The regime of artificial acid rain, in terms of spray frequency per monthly and spray amount at year records. Simulated acid rain (pH 2.0, pH 4.0, pH 5.6 as control) containing sulfuric and nitric acid in the ratio of 3:2 (chemical equivalant basis) diluted ground water, were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season (April 8 to August 31) in 1991. Seedling establishment, seedling growth and nutrient contents in needles and leaves were measured and compared among the treatment. The results were summerized as follows; 1. In general, coniferous species were more resistant than broad-leafed species in the resistant comparisons of landscape trees by simulated acid rain. But there were resistant tree species to acid rain among the broad-leafed species. 2. Among 6 coniferous species used in this experiment, Pinus densiflora, Pinus koraiensis, Taxus cuspidata, Cedrus deodara and Pinus thunbergii revealed the resistant tree species to acid rain. But Thuja occidentalis was very susceptible to acid rain, because of no germination in pH 2.0 treatment plot of acid rain. 3. Among 6 broad-leafed species used in this study, Magnolia obovata, Styrax obassia and Ligustrum obtusifolium showed relative resistant tree species to acid rain. But Acer Buergerianum, Acer Ginnala and Cornus Kousa were very susceptible to acid rain, because of no germination in pH 2.0 plot of acid rain treatment.

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Stem Rot of Sweet Potato (Ipomoea batatas) Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 고구마 흰비단병)

  • Kim, Ju-Hee;Kim, Shin-Chul;Cheong, Seong-Soo;Choi, Kyu-Hwan;Kim, Du-Yeon;Shim, Hong-Sik;Lee, Wang Hyu
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.118-120
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    • 2013
  • During the early spring of 2007 to 2009, stem rot of sweet potato (Ipomoea batatas L.) caused by Sclerotium rolfsii occurred in seedling stage grown in plastic film house at Iksan. In seedling stage of sweet potato, symptoms were initially appeared in yellowing and then the seedlings were eventually wilted. The fungus produced abundant white silky mycelium on infected tissues and soil line. Seedlings were very susceptible and died quickly once they were infected. The whole area of a petridish was rapidly covered with white mycelium on agar medium. Sclerotia began to produce after 7 days of mycelial growth and white sclerotia quickly melanized to a dark brown coloration. The causal agent isolated from the diseased plants was identified as Sclerotium rolfsii Saccardo on the basis of the morphological and cultural characteristics. All isolates of S. rolfsii caused similar symptoms on the host petioles by artificial inoculation.

Seedling Production and Rearing of Pale Chup, Zacco platypus (Temminck et Schlegel) (피라미, Zacco platypus (Temminck et Schlegel)의 종묘생산)

  • 남명모;최낙중;김성원;석규진;이종윤
    • Journal of Aquaculture
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    • v.12 no.1
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    • pp.25-30
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    • 1999
  • This study was carried out to produce the seedling of the larvae and juveniles of pale chup, Zacco platypus (Cyprinidae) in terms of artificial hatching, feeding behavior and growth rates. The total lengths of the newly hatched larvae were 7.6~8.2mm (mean: 7.97mm). The hatched larvae rose to the surface 3 days after hatching. The larvae were fed Daphnia, rotifer, Artemia and powdered feed. Hatching experiments were conducted in jar incubators under the different water temperature conditions, $20~32^{\circ}C$. The highest hatching rate was at $26^{\circ}C$ after 47 hours. Jar incubators was the highest hatching rate (89%), and cage and Californian incubators were useful and relatively high hatching rates (>89%). The size of the larvae 50 days after hatching were 30.0mm (25.6~32.0mm). As the water temperatures was increased, the growth rate of the larvae was also increased. Especially, the growth rates were favorable over $26^{\circ}C$, but survival was the worst at $32^{\circ}C$. This species was quick to accept assorted feed after hatching. The assorted feeds for flounder and rockfish better than that of carp in reference to growth rate.

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Studies on Pathogenicity of Wheat Scab Fungus(Gibberella zeae) to Various Crop Seedlings

  • Chung H. W.;Chung H. S.;Chung B. J.
    • Korean journal of applied entomology
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    • v.3
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    • pp.21-25
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    • 1964
  • 1. Purpose of this experiment is to investigate the influence of wheat scab fungus(Gibberella zeae) remaining in soil or residues of infected plants to the growth of the seedlings of 14 different crops. 2. Susceptibility of these crops to the infection by G. zeae was investigated by planting these crops both to the artificially inoculated soils and naturally infected soils where both serious and light wheat scab epidemic have occurred previously. 3. In artifical inoculation tests, bailey, wheat, rye, soybean, rice, buckwheat, corn, cotton and greenbean has shown susceptible reaction, while indianbean, sesame, sorghum, chinese cabbage and radish has shown resistant reaction. 4. In artificial inoculation tests, there was significant difference in the germination ratio of the susceptible crops between the plants planted in inoculated soils and uninoculated soils with the exception of rice, com and cotton. Preemergence seedling blight was confined only to barley and corn, whereas postemergence seedling blight was confined to rye, wheat, rice buckwheat, barley and corn. la most of the susceptible crops secondary roots were almost rotted and the primary roots were either partially rotted or discolorated in inoculated soil. There was significant difference in the stem length of the plants grown in inoculated soils and uninoculated soils in susceptible crops. 5. No infection by G. zeae was observed when wheat, barley and rye plants were sown to the soils where both serious and light wheat scab epidemic occurred naturally.

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Studies on the Artificial Seedling Production of Geoduck Clam, Panope japonica I. Spawning Induction and Hatching (코끼리조개의 인공종묘생산에 관한 연구 I. 산란유발 및 부화)

  • Lee, Chae-Sung;Rho, Sum;Park, Young-Je
    • Journal of Aquaculture
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    • v.10 no.2
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    • pp.113-121
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    • 1997
  • In order to developed the techniques for artificial seedling production of geoduck clam, Panope japonica, various stimulution for spawning induction and hatching condition were studied. Spawning induction by the air day or UV-irradiation stimulus were not effective. Water temperature stimulus was responsed 15.0~25.0% in May and 10.0% in June. But spawning induction by the gonad incision was highest with 15.0~45.0%. Ammonium hydroxide (NH4OH) stimulus adding in seawater were responsed 15.0% at 8/1000N~10/1000N, and ammonium hyhroxide solution injected in the gonads were responsed 5.0~10.0% at 5/100N~7/100N. The highest fertilization and hatching rate at various water temperature were ranged 74.5~89.2% in 11~$17^{\circ}C, \;84.3~89.5%\;in\;8~14^{\circ}C$, respectively. the highest fertilization rate and hatching rate in various salinity were ranged 72.5~88.5% in 25~$35tetperthousand$, 82.7~86.9% in 30~$35tetperthousand$, respectively. The optimum water temperature and salinity for fertilization and hatching to the 11~14$^{\circ}C$ and 30~$35tetperthousand$, respectively.

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Seedling Quality and Early Yield after Transplanting of Paprika Nursed under Light-emitting Diodes, Fluorescent Lamps and Natural Light (발광다이오드, 형광등 및 자연광 하에서 육묘된 파프리카의 묘소질 및 정식 후 초기 수량)

  • Lee, Jae Su;Lee, Hye In;Kim, Yong Hyeon
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.220-227
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    • 2012
  • This study was conducted to analyze the seeding quality of paprika and the growth and early yield after transplanting of paprika nursed under artificial light and natural light. In this study, blue LED, red LED, and white fluorescent lamps (FL) were used as artificial lighting sources. Photoperiod, average photosynthetic photon flux, air temperature, and relative humidity in a closed transplants production system (CTPS) were maintained at 16/8 h, $204{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 26/$20^{\circ}C$, and 70%, respectively. Leaf length, leaf width, leaf area, top fresh weight and dry weight of paprika seedlings, and chlorophyll content in paprika leaves nursed under LED and fluorescent lamps for 21 days after experiment were significantly affected by light treatments. As compared with the control (white FL), leaf area of paprika grown under blue LED, red LED, and natural light was decreased by 63%, 63%, and 28%, respectively. Top dry weight of paprika grown under blue LED, red LED, and natural light was 64%, 50%, and 22%, respectively, compared with the control. Number of leaves on 18 days after transplanting showed with red LED, blue LED, and natural light by 86%, 84%, and 48%, respectively, compared with the control. On 114 days after transplanting, paprika nursed under blue LED and red LED had relatively short plant height. This result might be caused that the elongation of its internodes was suppressed by the illumination of sole blue or red light. Average number of fruits per plant harvested during 4 weeks after first harvest was 3.5 with red LED, 3.3 with blue LED, 1.0 with natural light, and 2.2 with control, respectively. Early yield of paprika nursed under red LED, blue LED, natural light, and control were 453 g/plant, 403 g/plant, 101 g/plant, and 273 g/plant, respectively. Larger fruit of 136 g was harvested with red LED treatment. Even though the early yield of paprika was greatly increased with artificial lighting, but total yield was almost similar as the harvest period after transplanting in greenhouses was lengthened. From the above results, we could understand that paprika nursed under white FL, blue LED, and red LED showed good growth after transplanting and was early harvested by a week as compared to the natural light. Therefore, the white FL, blue LED, and red LED as the artificial lighting sources in CTPS could be strategically used to enhance the seedling quality, to shorten the harvest time, and to increase the yield of paprika.

Seedling Production of the Pacific Bluefin Tuna Thunnus orientalis (참다랑어 Thunnus orientalis의 종묘생산과 치어의 적정 사육수조 및 단백원 검토)

  • Ji, Seung-Cheol;Takaoka, Osamu;Takii, Kenji;Jeong, Gwan-Sik;Han, Seock-Jung
    • Journal of Aquaculture
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    • v.21 no.4
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    • pp.272-277
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    • 2008
  • We investigated the growth and survival in seedling production, and growth performance was compared with the different rearing tanks and protein source of formulated feed for juvenile Pacific bluefin tuna Thunnus orientalis (PBT). The survival rate at the end of nursery culture at 30 days after hatching was $0.69{\pm}0.40%$, and total length and mean body weight were $49.83{\pm}2.52\;mm$ and $1.03{\pm}0.09\;g$, respectively. Growth performance has no significant difference in fish reared by different tanks forms for 10 days. In order to develop an artificial diet, we evaluated the dietary utility of enzyme treated fish meal (Bio-CP, BIO) for juvenile PBT. Only diet BIO sustained similar growth and higher feed efficiency, and final carcass lipid content as compared to those of Sand lance (SL) These results revealed that BIO-CP a suitable dietary protein source, could sustain growth of PBT.

A Study on Penetration of Pea Seedling Taproots as Influenced by strength of Soil (토양(土壤)의 경도(硬度)가 완두뿌리의 신장(伸長)에 미치는 영향(影響))

  • Jo, In-Sang;Cho, Seong-Jin;Im, Jeong-Nam
    • Korean Journal of Soil Science and Fertilizer
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    • v.10 no.1
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    • pp.7-12
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    • 1977
  • This experiment was conducted in the laboratory in order to find out the relationships between the root growth and soil physical properties. The soils selected for this study were Sangju sandy loam, Yeongog loam, Hwadong silty clay loam, which have been considered to be a typical upland soils of Korea. Artificial core samples were made with various moisture contents and bulk densities. Elongation rate of pea seedling taproot and soil strength were measured respectively in these core samples. The results obtained are summarized as follows: 1. The soil strength increased with the bulk density and deceased with moisture content. 2. The correlation between root elongation and soil bulk density was significantly recognized at the same moisture content and the root elongation was influenced by the bulk density more significantly at dry condition. 3. The elongation rate of pea seedling taproot was significantly decreased by increasing the strength (Yamanaka tester and Fine probe) of the soils. 4. The soil strength of $21kg/cm^2$ in fine metal probe or 24mm in Yamanaka tester was considered to be the critical point for plant growth, which was restricting root elongation smaller than 1/4 of the maximum growth rate.

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Effect of Sodium in Artificial substrate on the Growth, Gas Exchange and Leaf Water Status of Cucumber (Cucumis sativa L.) and Korea Melon(Cucumis melo L.) (상토에 함유된 Na함량이 오이와 참외의 생육, 광합성 및 잎의 수분상태에 미치는 영향)

  • Seo, Young-Jin;Kim, Jong-Su;Kim, Chan-Yong;Park, So-Deuk;Park, Man
    • Korean Journal of Soil Science and Fertilizer
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    • v.41 no.3
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    • pp.177-183
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    • 2008
  • Sodium is known to reduce a plant growth and yields. However, the relationships between physiological response of seedling and salinity stress caused by growing media are not well understood yet. We conducted experiments to investigate change of some parameters including Na, EC, moisture content in media under different air temperature ($15^{\circ}C$, $25^{\circ}C$), and the response of fruit-vegetables such as cucumber, oriental melon on saline conditions originated from horticultural substrate. Volumetric moisture content of media at $15^{\circ}C$ was 70%, but at $25^{\circ}C$ was decreased by 45% within 22 hrs, showing below optimal matric potential, approximately. During reaction time, the increase of Na concentration was significantly greater in saline substrate than in control. The decrease rate of Na concentration according to supplying irrigation water was higher in saline substrate than in control. $CO_2$ assimilation rate and transpiration rate of Korea melon grown in low temperature were decreased with a Na/cation ratio in hydroponic solution. Water saturation deficit was also increased significantly at $15^{\circ}C$ as compare to $25^{\circ}C$. Saline stress during nursery stage induced a reduction of seedling quality, growth and cucumber yield. The results suggest that the relationship between uncontrolled Na uptake of seedling from saline substrate and meteological condition is responsible for saline stress.