• The Plant Pathology Journal
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• 제38권1호
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• pp.1-11
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• 2022

Fusarium culmorum is one of the most important causal agents of root rot of wheat. In this study, 10 F. culmorum isolates were collected from farms located in five agro-ecological regions of Morocco. These were used to challenge 20 durum wheat genotypes via artificial inoculation of plant roots under controlled conditions. The isolate virulence was determined by three traits (roots browning index, stem browning index, and severity of root rot). An alpha-lattice design with three replicates was used, and the resulting ANOVA revealed a significant (P < 0.01) effect of isolate (I), genotype (G), and G × I interaction. A total of four response types were observed (R, MR, MS, and S) revealing that different genes in both the pathogen and the host were activated in 53% of interactions. Most genotypes were susceptible to eight or more isolates, while the Moroccan cultivar Marouan was reported resistant to three isolates and moderately resistant to three others. Similarly, the Australian breeding line SSD1479-117 was reported resistant to two isolates and moderately resistant to four others. The ICARDA elites Icaverve, Berghisyr, Berghisyr2, Amina, and Icaverve2 were identified as moderately resistant. Principal component analysis based on the genotypes responses defined two major clusters and two sub-clusters for the 10 F. culmorum isolates. Isolate Fc9 collected in Khemis Zemamra was the most virulent while isolate Fc3 collected in Haj-Kaddour was the least virulent. This work provides initial results for the discovery of differential reactions between the durum lines and isolates and the identification of novel sources of resistance.

• 한국육종학회지
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• 제41권3호
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• pp.354-357
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• 2009

Venturia nashicola에 의한 검은별무늬병 저항성 품종을 육성하기 위하여 저항성 유전자원 탐색을 1997~1999년까지 국립원예특작과학원 포장검정 및 인공 접종을 실시하여 저항 성친으로 서양배 "청마려"을 선발하였다. 농촌진흥청 국립원예특작과학원 과수과에서 고품질 만생종 "만수"에 "청마려"를 1997년에 교배하여 저항성 우량 계통인 중간모본 "원교나-흑성 2호" 을 2008년에 선발하였다. 2008년 만개일은 4월 19일로 "만수" 보다 1일 늦고, "청마려"보다 7일 정도 빠르며 수세는 강하고 수자는 직립형이다. 검은별무늬병에 "청마려"와 "Bartlett"과 같이 강하고 "신고", "원황"등 국내 주요 재배 품종들과 교배 친화성이 있다. 과실은 만개 후 180일경에 성숙되어 양친에 비해 20일 정도 성숙 일수가 짧으며, 과형은 방추, 과피는 황녹갈색이다. 평균과중은 484 g, 당도는 $13.2^{\circ}Brix$이며 과즙량은 중~다이다 석세포가 적고 동양배의 아삭아삭함이 있다.

• Journal of Animal Science and Technology
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• 제59권10호
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• pp.21.1-21.6
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• 2017

Background: The aim of the study is to evaluate the effectiveness of thermographic monitoring, using the temperature changes of perianal and perivulvar areas for the determination of estrus in Anatolian Shepherd bitches. Fifteen bitches were used in the study. Blood and vaginal smear samples were collected and thermographic monitoring of perianal and perivulvar areas were carried out starting from proestrus to early diestrus. Also, external signs of estrus were investigated. Smear samples were evaluated by light microscopy after Diff-Quik staining method and superficial and keratinized superficial cells were determined as percentage (S + KS%). Progesterone and luteinizing hormone measurements were done by radioimmunoassay. The difference in temperature between perianal and perivulvar areas was evaluated through thermographic images by FLIR ResearchIR Software. Results: According to the results obtained from the study, differences between progesterone and S + KS% were statistically significant (P < 0,05). Although temperature showed increase and decrease with progesterone and S + KS%, the differences were not important statistically (P > 0,05). Serum luteinizing hormone levels did not sign any difference (P > 0,05). Conclusions: As a result, thermographic monitoring alone is not enough for estrus detection in Anatolian Shepherd bitches. However, it can be used to assist the actual estrus detection technique in terms of providing some foreknowledge by evaluating the differences in temperature.

• Asian-Australasian Journal of Animal Sciences
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• 제26권5호
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• pp.603-608
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• 2013

Hanwoo have been subjected over the last seventy years to intensive artificial selection with the aim of improving meat production traits such as marbling and carcass weight. In this study, we performed a signature of selection analysis to identify recent positive selected regions driven by a long-term artificial selection process called a breeding program using whole genome SNP data. In order to investigate homozygous regions across the genome, we estimated iES (integrated Extended Haplotype Homozygosity SNP) for the each SNPs. As a result, we identified two highly homozygous regions that seem to be strong and/or recent positive selection. Five genes (DPH5, OLFM3, S1PR1, LRRN1 and CRBN) were included in this region. To go further in the interpretation of the observed signatures of selection, we subsequently concentrated on the annotation of differentiated genes defined according to the iES value of SNPs localized close or within them. We also described the detection of the adaptive evolution at the molecular level for the genes of interest. As a result, this analysis also led to the identification of OLFM3 as having a strong signal of selection in bovine lineage. The results of this study indicate that artificial selection which might have targeted most of these genes was mainly oriented towards improvement of meat production.

• 한국환경복원기술학회지
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• 제21권1호
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• pp.95-102
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• 2018

Only the habitat characteristics and breeding status of Paridae have been studied, in addition to the lack of research on Parus varius varius, there is no study on the home-range in the post fledging period. This study was analyzed the home-range size of Parus varius varius in the post fledging period. The survey was conducted in the site located in Dankook Univ. Cheonan Campus(Middle Chungcheong Province). We captured five newborn Parus varius varius using artificial nest was installed before. Radio-tracking was carried out for analysis of home-range, and MCP (Minimum Convex Polygon) was used for analysis. We analyzed 1 individual tracked 15 days (VT5) and 4 individuals which missing radio-tracking transmitter within 3 days (VT1~VT4). Home-range of VT5 gradually increased to 1,38ha, 1.42ha, 2.14ha in the order of early, middle, late period. On the other hand, moving distance was decreased to 174.558m 125.129m, 120.180m. Home-range of V1~VT4 was estimated as 0.81ha which was 75.3% share of home-range of VT5 in early period. As the result it was found that home-range is formed far apart from artificial nest that has been influenced by human being interference, thereafter gradually adapting to interference and spreading close to artificial nest. through this research, we can construct basic ecological data for protecting habitat of Parus varius varius and increasing life rate. As first radio-tracking study of Parus varius varius home-range in the post fledging period, it is expected to be useful for the future study of home-range.

• 한국동물학회지
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• 제39권1호
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• pp.89-97
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• 1996

번식시기동안 상호 이소종인 노랑턱멧새와 붉은뺨멧새의 song을 충청북도 청원군 강내면에서 녹음하여 분석하였다. 두종의 수컷들을 대상으로 playback실험을 실시하였다. 노랑턱멧새와 붉은뺨멧새가 각각 자연적 그리고 인공적인 song repertoires로 playback을 하였을 때 이웃-낯선개체를 인식하는지의 여부를 조사하였다. 또한 붉은뺨멧새에 대해 전반부의 song과 후반부의 song을 playback하여 이웃-낯선개체의 인식 여부도 조사하였다. 노랑턱멧새는 자연적인 song repertoires로 이웃-낯선개체를 인식하였으나, 인공적인 song에 대해서는 반응하지 않았다. 붉은뺨멧새는 자연적, 인공적, 그리고 song의 전반부를 playback하였을 때 이웃-낯선개체를 구별하였다. 반면에 song의 후반부를 playback하였을 때는 반응하지 않았다.

• 환경영향평가
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• 제32권1호
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• pp.1-14
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• 2023

백로번식지 주변 개발사업을 진행할 때 환경영향평가 단계에서 갈등을 사전에 예방할 수 있도록 중점평가항목 선정과 저감방안을 제시하여 지속가능한 공존의 방안을 강구하고자 한다. 중점평가항목으로 백로번식지 보전을 위한 동·식물상 항목, 백로번식지로 인한 악취와 소음의 영향을 평가할 수 있는 악취와 소음 항목을 선정한다. 평가항목의 평가방안은 첫째, 동·식물상 항목에서 조사범위는 법정보호종 위주로 국한하지 말고 주요 종 번식지와 철새도래지에 해당하는 백로번식지와 취식지까지 포함하여 조사하고, 번식지와 취식지 면적감소에 따른 보전방안을 세운다. 둘째, 악취 항목에서 조사시기는 6월말에서 7월말까지 악취발생량이 최고인 시점을 포함한다. 악취 평가기준은 농도규제인 복합악취 배출허용기준과 사람이 느끼는 악취농도는 2~5OU/m³, 빈도는 2% 이하로 한다. 셋째, 소음 항목에서 조사시기는 백로류와 어린 새들의 소리가 합해져서 소음 발생이 최대가 되는 시점을 포함하여 5월부터 8월까지 매달 소음을 측정한다. 여름철새인 백로류의 소음은 일정 기간에만 발생하기 때문에 방음벽 설치 등 인공적인 구조물 설치로 소음을 저감하기 보다는 이격거리확보, 완충녹지, 마운딩 조성 등의 방법으로 저감대책을 세운다. 기존 백로번식지가 개체군의 적정한 밀도를 유지하면서 개체군이 다른 곳으로 이동하지 않기 위해서는 영소목 관리와 취식지의 양과 질을 안정적으로 유지해주는 것이 선행되어야 한다.

• 식물분류학회지
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• 제45권4호
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• pp.353-361
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• 2015

난초 열매의 결실의 시 공간적 변화가 장기적으로 개체군의 생존에 영향을 끼칠 수 있으며 수 세대에 걸쳐 유전적 다양성에 영향을 미칠 것이다. 본 연구의 목적은 화밀을 분비하는 육상 난초인 닭의난초를 대상으로 자연 조건에서 시 공간의 결실 수준을 조사하고 교배계를 파악하는 데 있다. 본 연구자들은 전라남도 해남군 징의리 해안 1.5 km 걸쳐 위치한 4곳 집단에서 2년간의 조사 기간 동안 수분실험을 행하였다. 2년 동안 결실률이 집단 내 작은 슈트의 모임인 패치 내에서 유사했다. 대조적으로, 패치 간에는 결실룰이 유의 한 수준에서 차이를 보였다. 또한 큰 화서를 지닌 식물이 작은 화서를 지니는 식물보다 훨씬 더 많은 열매 생성을 보였다. 닭의난초의 결실률은 2 년의 조사 기간 동안 비슷하였으나(14.1%) 연구된 보상을 주는(rewarding) 난초84종의 평균 결실률(37.1%) 보다 낮았다. 인위적인 자가 수분(90.5-95.2%), 인위적인 인화(隣花)수분(geitonogamy: 94.7-95.0%), 그리고 인위적인 타가 수분(91.3-91.4%)등에 의해서는 결실률이 상당한 수준으로 증가되었다. 꽃피기 전 수술을 제거 한 경우에 열매가 전혀 맺히지 않았으며 꽃 스스로 자동으로 수분이 일어나지 않았다. 본 연구 결과에 의하면 닭의난초는 자가화합성이고, 열매를 생성하기 위해서는 수분매개체인 곤충류가 필요하며, 2년간 조사 기간 중 징의리 집단들의 환경이 비슷하였음을 암시하고 있다. 본 연구 결과는 결실률이 시간보다 공간적으로 더욱 뚜렷하다는 점을 강조하고 있다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.69.2-70
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• 2003

For the selection and breeding of chestnut varieties resistant to the chestnut blight fungus Cryphonectria parasitica, disease resistance and susceptibility of 28 varieties widely planted and growing in Korea were evaluated by artificial inoculation of a pathogenic fungus. For this experiment, a typical virulent strain (KCPC-19) was selected. Artificial inoculation was conducted into all varieties by using two different materials and methods, i.e., bark and wood tissue sections in the laboratory and living trees in the field. In the bark and wood tissue section method, the size of necrotic area and canker development on chestnut varieties were examined and compared 4 days after inoculation. There were wide variations of chestnut varieties in disease resistance and susceptibility against chestnut blight fungus, but 3 varieties, Daebo！, Ishizuchi, and Sandae, were shown to be relatively resistant to the disease with the necrotic area of 0.95-1.03 cm2, while Arima was the most susceptible with the size of 2.0 cm2. In the living tree inoculation examined 5 weeks after inoculation, 3 varieties, Daebo, Ishizuchi, and Riheiguri, showed the higher resistance, but Tono 2 did the highest susceptibility among tested varieties.

• International Journal of Industrial Entomology and Biomaterials
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• 제13권2호
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• pp.63-72
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• 2006

Parthenogenesis, the development of unfertilized ovum opens new perspectives in silkworm breeding in the development of homozygous breeds. In order to improve induction of artificial parthenogenesis in the excised unfertilized eggs of different breeds of the silkworm, Bombyx mori L., a new method was devised and the results were compared with the routine method. General and specific combining abilities and hybrid vigour of newly developed bivoltine breeds were analyzed utilizing bivoltine breeds viz., $CSR_2,\;CSR_4,\;CSR_{17}\;and\;NB_4D_2$ Estimation of GCA revealed superiority of the breeds, $DNB_1$ for eight characters followed by $DNB_4$ for five characters. Among the testers, $CSR_2$ was found good general combiner for seven characters followed by $CSR_{17}$ for four characters. A great deal of variations was observed among the hybrids studied. Five hybrids namely, $DNB_1{\times}CSR_2,\;DNB_4{\times}CSR_4,\;DNB_4{\times}NB_4D_2,\;DNB_6{\times}CSR_2\;and\;DNB_7{\times}CSR_2$ showed significant SCA effects for 5-6 characters. The hybrid, $DNB_4{\times}CSR_4$ showed its superiority by expressing significant hybrid vigour over BPV for 7 characters. Majority of the hybrids exhibited significant hybrid vigour for survival rate, yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, filament length and denier.