• Title/Summary/Keyword: Artificial Agent

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Properties and Fabrication of Nanostructured 2/3 Cr-ZrO2 Composite for Artificial Joint by Rapid Sinerting (급속 소결에 의한 인공관절용 나노구조 2/3 Cr-ZrO2 복합재료 제조 및 특성)

  • Kang, Hyun-Su;Kang, Bo-Ram;Shon, In-Jin
    • Korean Journal of Materials Research
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    • v.24 no.9
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    • pp.495-501
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    • 2014
  • Despite having many attractive properties, $ZrO_2$ ceramic has a low fracture toughness which limits its wide application. One of the most obvious tactics to improve its mechanical properties has been to add a reinforcing agent to formulate a nanostructured composite material. Nanopowders of $ZrO_2$ and Cr were synthesized from $CrO_3$ and Zr powder by high energy ball milling for 10 h. Dense nanocrystalline $2/3Cr-ZrO_2$ composite was consolidated by a high-frequency induction heated sintering method within 5 min at $600^{\circ}C$ from mechanically synthesized powder. The method was found to enable not only rapid densification but also the inhibition of grain growth, preserving the nano-scale microstructure. Highly dense $2/3Cr-ZrO_2$ composite with relative density of up to 99.5% was produced under simultaneous application of a 1 GPa pressure and the induced current. The hardness and fracture toughness of the composite were 534 kg/mm2 and $7MPa{\cdot}m1/2$, respectively. The composite was determined to have good biocompatibility.

A Study on the Chemical Cleaning Process and Its Qualification Test by Eddy Current Testing

  • Shin, Ki Seok;Cheon, Keun Young;Nam, Min Woo;Min, Kyong Mahn
    • Journal of the Korean Society for Nondestructive Testing
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    • v.33 no.6
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    • pp.511-518
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    • 2013
  • Steam Generator (SG) tube, as a barrier isolating the primary coolant system from the secondary side of nuclear power plants (NPP), must maintain the structural integrity for the public safety and their efficient power generation. So, SG tubes are subject to the periodic examination and the repairs if needed so that any defective tubes are not in service. Recently, corrosion related degradations were detected in the tubes of the domestic OPR-1000 NPP, as a form of axially oriented outer diameter stress corrosion cracking (ODSCC). According to the studies on the factors causing the heat fouling as well as developing corrosion cracking, densely scaled deposits on the secondary side of the SG tubes are mainly known to be problematic causing the adverse impacts against the soundness of the SG tubes [1]. Therefore, the processes of various cleaning methods efficiently to dissolve and remove the deposits have been applied as well as it is imperative to maintain the structural integrity of the tubes after exposing to the cleaning agent. So qualification test (QT) should be carried out to assess the perfection of the chemical cleaning and QT is to apply the processes and to do ECT. In this paper, the chemical cleaning processes to dissolve and remove the scaled deposits are introduced and results of ECT on the artificial crack specimens to determine the effectiveness of those processes are represented.

Antifungal Effects of Some Antibacterial Antibiotics against Phytophthora nicotianae var. parasitica in Vitro (세균용(細菌用) 항생제(抗生劑)의 참깨역병균(疫病菌) (Phytophthora nicotianae var. parasitica)에 대(對)한 항균효과(抗菌效果))

  • Kim, Hong Gi
    • Korean Journal of Agricultural Science
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    • v.15 no.1
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    • pp.76-81
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    • 1988
  • This study was done to find out the antifungal activity of two antibacterial antibiotics, Chlorampenicol and Streptomycin sulfate, against Phytophthora n. var. Parasitica, the causal agent of Phytophthora blight of sesame, growing on artificial media. On PDA medium, Chlorampenicol at 10 ppm, Streptomycin sulfate at 25ppm highly inhibited mycerial growth and completely inhibited zoosporangial formation of Phytophthora n. var. parasitica, and Chlorampenicol at 5 ppm, Streptomycin sulfate at 10 ppm slightly inhibited the mycerial growth and zoosporangial formation of the fungus. These antibiotics showed considerably increased inhibitory effect on the fungal growth when they were mixed with other chemical. Protein content in myceria of the fungus was decreased and abnormal growth of mycerial apex was observed by treatment of these antibiotics. Phytotoxicity on sesame seedlings was not observed by application of them.

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THE INFLUENCE OF THE DEGREE OF SATURATION OF ACIDULATED BUFFER SOLUTIONS IN THE ROOT DENTIN DEMINERALIZATION (유기산 완충용액의 포화도가 상아질의 탈회에 미치는 영향)

  • Kang Hye-Sil;Lee Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.29 no.5
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    • pp.454-461
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    • 2004
  • The purpose of this study is to compare and to evaluate the effects of the degree of saturation on the progression of artificial root caries lesion. A total of 8 human premolars without any defects and cracks selected and the cementum were removed and the teeth were cleaned with ultrasonic device and pumice without fluoride. Each tooth was sectioned into 6 pieces and they were ground with #800 sandpaper until they had a thickness of 200pm. Specimens were applied with nail vanish except for the 2-3 mm window area after application of bonding agent. Under the constant pH, the specimens were divided into 6 groups (degree of saturation: 0.1415, 0.1503, 0.1597, 0.1676, 0.1771. 0.1977). Each group was immersed in acid buffer solution for 1. 2, 3, 5 days under controlled temperature ($25^{\circ}C$) and imbibed in water and examined using the polarizing microscope. The results were as follows 1. Although the degree of saturation of demineralization solution decreased, the depth of penetration in the dentin was constant. 2. Erosion was observed on the surface of all the teeth in the group I, II. In the group III, IV, V, surfaces were not changed. The teeth in the group VI showed the more mineralized surface but not the shape of the dentinal tubules distinctively. 3. In all groups, the lesion progressed rapidly at the first day of the experiment, but increased gradually as time elapsed.

Expression and Activation of Akt/PKB Protein Kinase using Escherichia coli (대장균을 이용한 Akt/PKB Protein Kinase의 발현 및 활성화)

  • Lee, Jae-Hag
    • Microbiology and Biotechnology Letters
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    • v.37 no.2
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    • pp.105-109
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    • 2009
  • Among signal transduction systems by protein phosphorylation Akt/PKB protein kinase which is one of serine/threonine kinases, is known to regulate the survival and death of the cell and glucose metabolism. Thus, Akt/PKB protein kinase has been used as one of the target proteins to find anti-cancer agents from natural products. In this study, human Akt/PKB protein kinase was expressed in Escherichia coli expression system for the mass production. Human Akt/PKB protein kinase expressed in E. coli formed inclusion body under the general condition. However, most of the expressed protein was solubilized under the culture temperature at $27^{\circ}C$ and 0.01-0.09 mM of IPTG for induction of the protein expression. The expressed protein was purified using $Ni^{2+}$-NTA agarose column and confirmed by using anti-Akt antibody. Subsequently, the purified human Akt/PKB protein kinase was activated by in vitro phosphorylation using cellular extract containing kinases. The activated protein was confirmed to phosphorylate the specific fluorescent peptide specially designed as the artificial substrate for Akt/PKB protein kinase.

Cryopreservation of Common Korean Bitterling Acheilognathus signifer Sperm (묵납자루(Acheilognathus signifer) 정자의 냉동보존)

  • Jeong, Min Hwan;Min, Byung Hwa;Park, Mi Seon;Myeong, Jeong-In;Im, Je Hyun;Lim, Han Kyu;Hwang, Hyung-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.1
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    • pp.39-44
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    • 2014
  • This study aimed to find an optimal diluent and cryoprotective agent (CPA) during cryopreservation of common Korean bitterling Acheilognathus signifier sperm. The bitterling is an endangered species in Korea, and this study will enable conservation and further technical development of artificial seed production. We tested the effects of cryopreservation and toxicity by the type of diluent and CPA. The optimal combination of diluent and CPA for cryopreservation was 300 mM glucose+10% methanol, resulting in a survival rate and sperm activity index (SAI) of $96.3{\pm}1.5%$, and $3.0{\pm}0.0$ respectively, and no significant difference compared to fresh sperm. The survival rate and SAI of post-thawed sperm was $9.7{\pm}1.5%$, $0.8{\pm}0.3$ respectively, which was significantly higher than had been achieved with other diluents and CPAs.

The Ethics of Robots and Humans in the Post-Human Age (포스트휴먼 시대의 로봇과 인간의 윤리)

  • You, Eun-Soon;Cho, Mi-Ra
    • The Journal of the Korea Contents Association
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    • v.18 no.3
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    • pp.592-600
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    • 2018
  • As the field of robots is evolving to intelligent robots that can replace even humans' mental or emotional labor, 'robot ethics' needed in relationship between humans and robots is becoming a crucial issue these days. The purpose of this study is to consider the ethics of robots and humans that is essential in this post-human age. It will deal with the followings as the main contents. First, with the cases of developing ethics software intended to make robots practice ethics, the authors begin this research being conscious about the matter of whether robots can really judge what is right or wrong only with the ethics codes entered forcibly. Second, regarding robot ethics, we should consider unethicality that might arise from learning data internalizing human biasness and also reflect ethical differences between countries or between cultures, that is, ethical relativism. Third, robot ethics should not be just about ethics codes intended for robots but reflect the new concept of 'human ethics' that allows humans and robots to coevolve.

A Target Leaf Spot Disease Caused by Corynespora cassiicola on Cucumber Cultivated in Green House (시설하우스에서 Corynespora cassiicola에 의해 발생하는 오이 갈색무늬병)

  • Kwon, Mi-Kyung;Yang, Kwang-Yeol;Cho, Baik-Ho
    • Research in Plant Disease
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    • v.10 no.2
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    • pp.121-125
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    • 2004
  • An epidemic of target leaf spot of cucumber (Cucumis sativus) occurred in commercial greenhouses in Korea in 2000/2001. The early symptoms on the leaves were small brown spots with yellow halos. These lesions became irregular enlarging in diameter and eventually defoliation resulted. The causal agent was a fungus with morphological characteristics matching Corynespora cassiicola. The sequence of the ITS region of C. cassiicola CM2000-1 was identical to that of an authentic strain of Corynespora cassiicola. Optimal germination of spores and mycelial growth on plate was at 3$0^{\circ}C$. A long dew period on the leaf surface and high temperatures were the main contributing factors for disease development and the greenhouse epidemic. Artificial inoculation of the Korean isolate of C. cassiicola revealed resistance in some Korean cucumber cultivars.

Halo Blight of Kudzu Vine Caused by Pseudomonas syringae pv. phaseolicola in Korea

  • Jeon, Yong-Ho;Chang, Sung-Pae;Kim, Sang-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.119-124
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    • 2006
  • Kudzu vine(Pueraria montana var. lobata) is an invasive climbing woody vine that envelops trees and shrubs, pressing physically and shutting out sunlight, which needs to be controlled. Kudzu vine pathogens were surveyed as a way to seek its biocontrol agents in 2002. Occurrence of a bacterial halo blight disease of kudzu vine was observed at several localities in Korea including Euiwang and Suwon in Gyeonggi Province, Daejon, and Gochang and Buan in Jeonbuk Province. Symptoms of brown to black spots with a surrounding yellowish halo appeared from June and lasted till the rainy season without much expansion, but accompanying often leaf blight and defoliation. Isolated bacteria were identified as Pseudomonas syringae pv. phaseolicola based on physiological and cultural characteristics, Biolog, fatty acid and 16S rDNA sequencing analyses. In artificial inoculation test, these bacteria produced the same halo spot symptoms on kudzu vine and bean plants. They also induced hypersensitive responses (HR) on tobacco, tomato, and chili pepper leaves. This is the first report of a bacterial disease of kudzu vine in Korea, and the bacterial pathogen can be used as a biocontrol agent against the pest plant.

Stem Rot of Sweet Potato (Ipomoea batatas) Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 고구마 흰비단병)

  • Kim, Ju-Hee;Kim, Shin-Chul;Cheong, Seong-Soo;Choi, Kyu-Hwan;Kim, Du-Yeon;Shim, Hong-Sik;Lee, Wang Hyu
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.118-120
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    • 2013
  • During the early spring of 2007 to 2009, stem rot of sweet potato (Ipomoea batatas L.) caused by Sclerotium rolfsii occurred in seedling stage grown in plastic film house at Iksan. In seedling stage of sweet potato, symptoms were initially appeared in yellowing and then the seedlings were eventually wilted. The fungus produced abundant white silky mycelium on infected tissues and soil line. Seedlings were very susceptible and died quickly once they were infected. The whole area of a petridish was rapidly covered with white mycelium on agar medium. Sclerotia began to produce after 7 days of mycelial growth and white sclerotia quickly melanized to a dark brown coloration. The causal agent isolated from the diseased plants was identified as Sclerotium rolfsii Saccardo on the basis of the morphological and cultural characteristics. All isolates of S. rolfsii caused similar symptoms on the host petioles by artificial inoculation.