• Korean Journal of Medicinal Crop Science
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• v.9 no.2
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• pp.116-123
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• 2001

This study was carried out to investigate antioxidative, antimicrobial activity and the effect on hepatotoxicity in various extracts of Artemisia iwayomogi. The herb has been used widely for jaundice, hepatitis and liver cirrhosis in chinese medicine. Solid yield by various extraction solvents, 18.1%, was the highest in water extract. To find antioxidative activity in Artemisia iwayomogi was estimated radical scavenging effect by DPPH method in various extracts and change of the POV(peroxide value) of various extracts added in soybean oil during 20 days at $60^{\circ}C$. Radical scavenging effect by DPPH method was the most effective in methanol extract. Added 1,000ppm water extract and methanol extract in soybean oil, the POV of them, 46.8(meq/kg) and 50.8(meq/kg) were lower than that of control, 79.1(meq/kg), during 20 days storage. After antimicrobial activity of various extracts of Artemisia iwayomogi on bacteria was carried out by paper disc method, it found that the ethanol extract was the strongest activity on Vibrio parahaemolyticus. In vivo experiment was to investigate the effect of Artemisia iwayomogi water extract(AIWE) on hepatotoxicity by carbon tetrachloride$(CCl_4)$ in rats. The experiment groups were divided into five groups for recovery(for 3 days) and three groups for protection(for 10 days) in rat liver. The weights and morphological changes of liver and the body weight were examined in each groups. Compared with $CCl_4$ treatment groups$(CCl_4\;only)$, liver and liver/body(%) weights of AIWE pretreatment groups for 3 days and AIWE posttreatment groups for 10 days were declined. In macrography, fibrious exudates and swelling of liver were decreased in AIWE treatment groups. Accumulation of lipid droplets and necrosis of hepatocytes were also decreased in AIWE treatment groups in microscopically. In these results, AIWE seems to enhance hepato-protective and recoverable effect on $CCl_4$ induced hepatotoxicity in rats.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.6
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• pp.153-163
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• 2009

This study was carried out to offer ecological restoration plan of local streams by analyzing the vegetation and flora around four local streams (Pungchoncheon, Namchangcheon, Ganggicheon, Woncheoncheon) in Namwon. Flora and vegetation investigation was done at April 2007 to August 2007, and June 2009. The 194 of vascular plants were identified which contain 53 families, 143 genera, 194 species. The Family frequencies arranged by the order of Family Compositae (14.4%), Family Gramineae (13.4%) Family Leguminosae (6.7%) and Family Polygonaceae (5.2%). The naturalized plants were listed as 33 species, and their naturalization ratio and urban index were found to be 17.0%, and 12.2%, respectively. In this study a total of 21 relev$\acute{e}$s collected and analyzed. 6 plant communities are differentiated : Riverine softwood forests (Phragmites japonica-Salix gracilistyla community), Perennial herb vegetations on lotic water zone (Phragmites japonica community, Phalaris arundinacea community), Perennial herb vegetations on lentic water zone (Persicaria thunbergii-Typha orientalis community, Phragmites communis community), Annual or biennial vegetations on waterside (Persicaria thunbergii community). Species showing the higer value in the plant communities were Persicaria thunbergii, Phragmites japonica, Artemisia princeps, Humulus scandens, Bidens frondosa, Oenanthe javanica, Agropyron tsukusinense var. transiens, Stellaria aquatica, and Lactuca indica var. laciniata.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.1
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• pp.22-27
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• 2004

In the present study, the protective effects of Artemisia capillaris (AC) on the DNA damage induced by $^{60}$ Co ${\gamma}$-rays were evaluated using alkaline single-cell gel electrophoresis (SCGE, comet assay) in the mouse peripheral lymphocytes and micronuclei (MN) formation test in the Chinese hamster ovary (CHO) cells. We also investigated the effect of AC on 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation in the mouse liver and thymus exposed to ${\gamma}$-ray, The tail moment and the frequency of MN, which were markers of DNA damage in the SCGE and MN formation test, were decreased in the groups treated with AC extract before exposure to 200 cGy of ${\gamma}$-ray. We also observed its activities, lowering 8-OHdG level, an index of oxidative DNA damage, in the groups treated with AC extract before whole body ${\gamma}$-irradiation (800 cGy). It is plausible that scavenging of free radicals by AC may have played an important role in providing the protection against the radiation-induced damage to the DNA. These results indicated that AC protects the DNA damage induced by ${\gamma}$-rays and might be a useful radioprotector, especially since it is a relatively nontoxic product.

• Journal of Life Science
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• v.14 no.2
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• pp.215-220
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• 2004

We investigated the anti-inflammatory effects of aqueous extract of Artemisia capillaris Thunb. (AEAC), a traditional Korean herb for remedying liver disease, for suppression in the process of lipopolysaccharide (LPS)-induced inflammation in the liver of rat. Level of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and lactate dehydrogenase (LDH) was increased in the serum of LPS-treated rats compared to normal, however, in the rats pretreated with AEAC, the increase of GOT, GPT and LDH value was arrested. More severe histological changes of liver such as cloudy swelling, hydropic degeneration, Kupffer cell reaction and inflammatory cells infiltration were demonstrated in the rats challenged with LPS compared with normal. Fewer scores of these changes were observed in rats pretreated with AEAC. Immunohistochemical analysis showed that while the expression of the nuclear factor (NF)-kBp65, inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-$\alpha$ and COX (cyclooxygenase)-2 tended to increase, that of inhibitory (I)-kBa was decreased in the hepatocytes of rats challenged with LPS. A slight decline of NF-kBp65, TNF-$\alpha$ and COX-2, but increase of I-kB$\alpha$ were observed in the hepatocytes of the rats pretreated with AEAC. These results suggest that AEAC may act as a therapeutic agent for liver disease through a regulation of inflammation-related proteins.

• The Journal of Korean Medicine
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• v.43 no.1
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• pp.18-32
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• 2022

Objectives: This study aimed to examine the safety, effects on proliferation of hair papilla cells, and anti-inflammatory and antioxidant mechanisms of Artemisia sieversiana Ehrh. ex Willd. (AS) extract. Methods: Safety tests through purity testing, acute toxicity tests, and repeated toxicity tests were performed using AS extract (ASE) which had been dried for over two years. Cell culture and proliferation tests were conducted; VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor), and EGF (epidermal growth factor) and protein expression analyses were performed for mechanistic evaluation; and inhibitory effects of ASE on the RNA expression of testosterone, 5𝛼-reductase, and aromatase was assessed. The anti-inflammatory and antioxidant efficacy of ASE was confirmed by measuring the levels of nitric oxide, inflammatory mediators (TNF-𝛼 and PGE2), inflammatory cytokines (IL-1𝛽, IL-6, and IL-8), and chemokine MCP-1. Results: The safety of ASE was confirmed. The mechanism of cell proliferation in human hair follicle dermal papilla cells involved the promotion of VEGF, bFGF, and EGF expression. ASE decreased mRNA expression of testosterone, 5𝛼-reductase, and aromatase-1 in a concentration-dependent manner. PGE2 and TNF-𝛼 production by inflammatory mediators was also significantly decreased in a concentration-dependent manner, and inflammatory cytokine and chemokine expression was inhibited. Conclusions: ASE is suggested to promote papillary cell growth at the cellular level, to suppress expression of various enzymes involved in hair cycle and cell death, and to inhibit hair loss through anti-androgen, anti-inflammatory, and antioxidant effects.

• The Journal of Internal Korean Medicine
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• v.26 no.4
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• pp.853-863
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• 2005

Object : This study was performed to investigate the anti-fibrogenic effect of Artemisiae Capillaris Herba(ACH) on cultured rat hepatic stellate cells. Methods : Hepatic Stellate Cells were obtained from a 350gm Sprague-Dawley rat by tissue perfusion system, and the cells for the study were selected after 3 passages of culture on non-coated plastic culture dishes which enable the cells to activate, thus producing collagens in the cell media. Cells were treated with various concentrations of Artemisia Capillaris Herba(ACH) extract powder for 24 or 48 hours. After the treatment, Soluble collagen, procollagen levels and the mRNA of the procollagen type I C were measured by using assay kit and RT-PCR method. Results : Procollagen production by the hepatic stellate cells decreased after the treatment in a time-dependent dose-dependent manner. The mRNA expression decreased consistently with the volume of the secreted procollagen which indicates the herb hat inhibitory effect on fibrogenesis of the liver by regulating one of the fibrosis associated genes in transcription. Conclusion : These results suggest that ACH is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.

• The Journal of Korean Medicine
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• v.22 no.1
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• pp.90-95
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• 2001

목적：인진 약침액이 SK-N-MC 신경아세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향을 조사하였다. 방법 : SK-N-MC cell line에서의 아폽토시스 변화를 관찰하기 위해서 MTT assay, DAPI staining 및 flow cytometric analysis 방법을 이용하였다. 결과： MTT assay를 이용하여 분석한 결과 농도에 따른 세포 독성의 효과가 에탄올 투여로부터 관찰되었다. 또한 인진 약침액으로 전처치하고 에탄올을 처치하였을 때 세포 독성이 크게 감소되었다. DAPI staining에서 인진 약침액 투여군은 에탄올 투여군에 비해서 fragmentation이 억제되었다. Flow cytometry를 통하여 인진 약침액 투여군은 에탄올 투여군에 비하여 세포주기 중 sub $G_1$ 분획의 증가가 억제되었다. 결론 : SK-N-MC 신경아세포주에서 에탄올에 의해서 유발된 아폽토시스는 전형적인 세포사별 형태를 나타내었다. 또한 인진 약침액은 에탄올에 의해서 유발된 아폽토시스에서 세포보호 효과가 있음이 확인되었다.

• Journal of Plant Biology
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• v.15 no.1
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• pp.1-12
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• 1972

As part of the study of the structure and the successional trends of our temperate forests, a quantitative survey was made of the relatively well conserved forest stands on Mt. Kwanak, Seoul. Three community types were observed; the Pinus densiflora-Quercus mongolica community, the Pinus densiflora community, and the Quercus mongolica community. Evidence suggests that the dominance of Pinus densiflora and quercus mongolica will continue in the Pinus densiflora-Quercus mongolica community, however, in the Pinus densiflora community the dominant species, Pinus densiflora, is now being replaced by Quercus mongolica. The trend toward an increased in Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus cordita, and Acer mono is observed in the Quercus mongolica community. An analysis of the overall composition of the forest reveals that the upper story is mostly occupied by Quercus mongolica and Pinus densiflora, the shrub layer by Rhododendron schlippenbachii and Lespedeza maximowiczii, and the herb layer by Spodiopogon cotulifer and Artemisia keiskeana. Quercus mongolica may cover most part of the forest, gradually excluding Pinus densiflora. Acer pseudo-sieboldianum var. koreanum, Fraxinus rhynchophylla, Carpinus coradata, and Acer mono are increasing in importance with the potentiality of developing into major canopy components. With the diversification of the physical habitat Cephalotaxus koreana and Magnolia sieboldii, the species which had almost completely been eliminated form the forest through the severe interferences, have reappeared.

• Journal of Sasang Constitutional Medicine
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• v.16 no.2
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• pp.128-133
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• 2004

Objectives Generally speaking, we could've not been used moxibustion to Soyangin. But we thought what matters is that which point is chosen, not that what method is chosen. So we had a case of moxibustion at Ki1 for a Soyangin patient with insomnia Methods We managed a patient with insomnia after intracranial hemorrhage. He had been undergone conservative therapy, herb medicine, acupuncture, sedatives. 20 days after admission we started to do moxibustion at both Ki 1 point(湧泉). We used artemisia and, the method was direct form. Results The duration and the quality of sleep of the patient was improved. After 10 days, he could sleep for 5 hours. Accompanied symptoms, as mentality, urination, and defication, was also improved. Conclusions From this case we could accept the efficacy of moxibustion at Ki 1 for Soyangin patient suffering from insomnia.

• Journal of Korean Society of Forest Science
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• v.85 no.3
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• pp.347-359
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• 1996

This study was carried out to find the plant succession stage on highway cut-slopes. In order to analyze succession stage, plant survey with belt transect method was carried out in 1989 and 1995 at Joongbu-highway cut-slopes. The results could be summarized as follows ; The mean plant coverage of cut-slope was 78.3%. Plant coverage of Lower part area was higher than that of upper part area. Pioneer herb species on cut-slope were Miscanthus spp., Arundinella spp., Artemisia spp. etc., and pioneer woody species were Rubus crataegifolius, Robinia pseudoacacia, Pueraria thunbergiana, Pinus spp. etc. Also, among the introduced turf grasses, Eragrostis curvula is the pioneer species. Due to short of maintenance works, Zoysia japonica which has been planted by sodding work was found to decrease or diminish gradually. The ratio of pioneer stage plants, domestic and introduced herb species, seems to decrease, while the ratio of woody species which are advanced stage plants seems to increase. Maintenance works are needed to management and monitoring of plant succession on cut-slope, and use of herb and woody species will be effective.