• 한국임상수의학회지
• /
• 제24권3호
• /
• pp.372-378
• /
• 2007

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain hepatitis chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillarix extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells on in vivo experimental tests. On in vivo experimental tests using 280 ICR mice the gain of body weight in the control-group mice bearing Sarcoma 180 ascites tumor was 1.5 times more than that of the normal-group mice after 33 days. However, the gain of body weight in all experimental groups administered with Artemisia capillaris extracts was significantly lower than that of the control-group mice (P<0.05). The mean survival times of mice administered with Artemisia capillaris extracts of 25 and 100 mg/kg for 28 days were shown to be 25.39% and 15.39% longer than that of the control-group mice injected with saline (P<0.05). Artemisia capillaris extracts showed the highest tumor inhibition effects, which were 42.4% and 27.2% when intraperitoneally injected with doses of 25 and 100 mg/kg once a day for 28 days in inoculated ICR mice with Sarcoma 180 solid tumor cells (P<0.05). The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• Journal of Nutrition and Health
• /
• 제41권1호
• /
• pp.22-30
• /
• 2008

사철쑥 (Artemisia capillaris Thunberg)이 암세포의 세포고사에 미치는 영향을 보기 위하여 인체 자국경부 상피암 세포주인 HeLa 세포를 이용하여 사철쑥의 메탄올, 에틸아세테이트, 부탄올 추출물의 농도를 각각 0, 100, 500, 1,000 및 $2,000{\mu}g/ml$로 처리하여 세포활성도, 세포형태 변화, DNA분절, DNA 함량을 알아보았다. 세포활성도는 사철쑥의 메탄올, 에틸아세테이트 추출물이 농도에 비례하여 HeLa세포생존율을 감소시켰지만 부탄올 추출물에서는 그 정도가 약하였고 물 추출물에서는 거의 관찰되지 않았다. 사철쑥의 메탄올, 에틸아세테이트, 부탄올과 물 추출물은 각 추출물의 농도에 의존적으로 배양된 HeLa 세포에 세포부착을 방해하고 세포막의 blobbing 및 핵의 분절화 등 세포고사의 특징들을 나타냈다. 사다리형 DNA 분절현상은 사철쑥의 메탄올 추출물 $1,000{\mu}g/ml$, $2,000{\mu}g/ml$ 처리군에서 관찰되었다. 유세포분석기 (FACS)를 이용하여 측정한 고사세포의 DNA 함량은 메탄올과 에틸아세테이트, 부탄올 추출물이 농도 의존적으로 증가하였다. 이상의 결과는 사철쑥이 암세포의 세포고사를 증가시킴으로서 암 억제 작용이 있을 것을 시사해 준다고 할 수 있다.

• Natural Product Sciences
• /
• 제5권1호
• /
• pp.1-6
• /
• 1999

This study was devised to observe the antitumor activity of mugwort (Artemisia princeps Pampan.) against ICR mice inoculated with sarcoma-180 cells. The antitumor compounds were partially purified from petroleum ether extract of mugwort by silicic acid column chromatography. The active fraction used in in vivo test was obtained under the elution with acetone in silicic acid column chromatography. When the acetone fraction was intraperitoneally injected to the mice which had been subcutaneously inoculated on the left groin with sarcoma-180, the growth rate of tumor (sarcoma-180 mass) was inhibited by 30%. In case the acetone fraction was injected to the mice which had been inoculated intraperitoneally with sarcoma-180, the average life span was prolonged by 20%. After the injection of the active fraction, the spleen index and ${\gamma}-globulin$ ratio (%) were increased significantly (p<0.05). The administration of acetone fraction did not cause any abnormality in the body and the homeostasis of mice. Those observations suggest that the acetone fraction of mugwort extract has an antitumor effect in vivo.

• 생약학회지
• /
• 제36권4호통권143호
• /
• pp.273-277
• /
• 2005

In mammalian melanocytes, melanin synthesis is controlled by tyrosinase, the key enzyme in the pigment synthesis. In this study, to develop a new whitening agent, we have investigated the antioxidant and the inhibitory effect of Artemisia anomala extract on tyrosinase activity and melanigenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of butanol fraction from A. anomala was higher than that of arbutin ($97.5{\pm}0.5%$ at the concentration of 2 mg/ml). The butanol fraction was shown scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also butanol fraction ($25.0{\pm}3%$ at the concentration of $200\;{\mu}g/ml$). From these results, we suggest that the A. anomala extract might be used to be a potential agent for skin whitening.

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권1호
• /
• pp.21-27
• /
• 2015

The anti-inflammatory, antioxidant, and antimicrobial properties of artemisinin derived from water, methanol, ethanol, or acetone extracts of Artemisia annua L. were evaluated. All 4 artemisinin-containing extracts had anti-inflammatory effects. Of these, the acetone extract had the greatest inhibitory effect on lipopolysaccharide-induced nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and proinflammatory cytokine ($IL-1{\beta}$, IL-6, and IL-10) production. Antioxidant activity evaluations revealed that the ethanol extract had the highest free radical scavenging activity, ($91.0{\pm}3.2%$), similar to ${\alpha}$-tocopherol (99.9%). The extracts had antimicrobial activity against the periodontopathic microorganisms Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum subsp. animalis, Fusobacterium nucleatum subsp. polymorphum, and Prevotella intermedia. This study shows that Artemisia annua L. extracts contain anti-inflammatory, antioxidant, and antimicrobial substances and should be considered for use in pharmaceutical products for the treatment of dental diseases.

• Natural Product Sciences
• /
• 제26권4호
• /
• pp.268-278
• /
• 2020

In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vitro, and the protective effects on gastric lesions in vivo. The IC50 of 2,2-diphenyl-1-picrylhydrazyl free radical scavenging considering the antioxidant effects of RW0117 was 166.55 ㎍/mL, and the IC50 of nitric oxide scavenging considering the anti-inflammatory effects was 41.16 ㎍/mL. Oral administration of RW0117 at lower concentrations (25, 50, 100 mg/kg) had similar or greater effects than the daily intake conversion concentration (115mg/kg) of a health functional food (Avexol®) in the acetic acid-induced ulcer and the ethanol-induced gastric injury rat models. In addition, oral administration of RW0117 increased the expression of prostaglandin E2, which enhances the protective effect in the gastric mucosa in the ethanol-induced gastric injury rat model. These results suggest that RW0117 may have potential therapeutic uses in the protection of the gastric mucosa.

• 한방재활의학과학회지
• /
• 제21권1호
• /
• pp.23-33
• /
• 2011

Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.

• 한국약용작물학회지
• /
• 제17권4호
• /
• pp.286-292
• /
• 2009

The aim of this study was to investigate the effect of plant growth at several different growing periods on antioxidant activities and zeatin and ABA contents of Artemisia iwayomogi. Measurements of antioxidant activities, lipid peroxidation inhibition, and superoxide radical scavenging activity were done using PMS, NBT and lipid auto-oxidation analysis, respectively. The results show that activities of antioxidants from Artemisia iwayomogi had much higher than BHT. DPPH free radical scavenging effect of Artemisia leaf extract was increased from $71.06{\pm}4.36%$ in April to $90.06{\pm}4.41%$ in October. Activities of superoxide radical scavenging and lipid peroxidation inhibition were $33.83{\pm}3.45%$ and $45.60{\pm}3.10$ in April and then increased to $84.40{\pm}4.00%$ and $75.86{\pm}3.50%$ in October, respectively. An ELISA technique has been developed for the determination of zeatin and ABA in Artemisia leaves. By this method, the content changes of zeatin and ABA from Artemisia during the growth were investigated. The zeatin content in leaf was measured to be $186.86{\pm}1.40$ pmol/g dry weight in April, however, decreased to $117.93{\pm}5.83$ pmol in October. The ABA content in leaf increased from $19.00{\pm}1.26$ nmol in April to $68.20{\pm}2.52$ nmol in October. Relationship between antioxidant activities and plant hormone contents was indicated that antioxidant activity may depend on decreasing zeatin content or increasing ABA content.

• KSBB Journal
• /
• 제15권4호
• /
• pp.331-336
• /
• 2000

여름과 가을에 수확한 연진쑥을 열수 빛 에탄올로 추출하 여 B(a)P의 변이원성에 대한 억제효과를 SOS Chromotest로 시험한 결과 여름에 수확한 시료의 물 추출물에서 강한 억제 효과를 나타내었다. 따라서 에탄올 가용성 분획과 불용성 분 획으로 분리하였으며 분획별 돌연변이 억제효과는 불용성 분 획에서 더 높게 나타났다. 불용성 분획은 SOS Chromotest외 A Ames test에서 정확한 용량의존성 억제효과를 나타내었고, 50% 돌연변이 억제농도 $(IC_{50}$는 E. coli PQ37에 대하여는 $200{\mu}g/assay$, S. typhimurium TA98에 대하여는 $800{\mu}b/plate$ TAIOO에서는 $600{\mu}g/plate$이었다. 그러나 세포내.외 항돌연변이 효과를 비교한 결과 세포내 항돌연변이 효과는 나타내지 않았다 따라서 인진쑥 물 추출물의 돌연변이 억제효과는 d desmutagenic effect에 의한 것으로 확인되었다 HPLC를 사용 하여, B(a)P의 변이원성에 주된 효소인 cytochrome P-4S0 1A1에 의해 대사되어지는 aflatoxin Mj 농도를 측정한 결 과 AFM1의 형성이 크게 감소되었다. B(a)P의 변이원성에 대 한 인진쑥 불추출물의 항돌연변이 효과는 아마도 B(a)P를 ultimate mutagen으로 대사시키는 cytochrome P-4S0 1A1 효소계를 저해하여 나타나는 것으로 해석된다.

• 한국식품영양과학회지
• /
• 제45권12호
• /
• pp.1708-1716
• /
• 2016

Akt 및 mTOR는 세포 생존에 필수적인 경로로 세포 성장과 증식 등에서 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 항암 및 항균 효과가 있는 것으로 알려진 개똥쑥(Artemisia annua L.)에 의한 HepG2 간암세포의 apoptosis 유도 효과를 확인하였다. 본 연구 결과에 의하면 개똥쑥 추출물의 처리 농도가 증가함에 따라 HepG2 세포의 생존율은 억제되었으며, 이는 apoptosis 유도 효과에 의한 것임을 세포의 형태적 변화와 flow cytometry를 통해 확인하였다. 그리고 mitopotential assay와 caspase-3/7 activity assay, western blotting으로 Bcl-2 family 단백질을 확인함으로써 apoptosis 경로 중 내인성 경로(intrinsic pathway)에 의해 apoptosis가 일어남을 알 수 있었다. 이러한 효과는 Akt/mTOR의 활성 저해와 연관이 있었으며 Akt/mTOR의 저해제인 LY294002/rapamycin을 개똥쑥 추출물과 병행처리하였을 경우 개똥쑥 추출물에 의한 apoptosis 효과를 더욱 증대시켰다. 따라서 Akt/mTOR의 저해는 개똥쑥 추출물의 apoptosis 효과를 상승시켰으며 이에 따라 미토콘드리아의 기능 손상과 caspase 활성의 증가를 통해 이루어짐을 확인하였다.