Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Artemisia Capillaris Thunberg on Apoptosis in HeLa Cells

사철쑥의 HeLa 세포고사 효과

  • Lee, Hyoung-Ja (Korea Foods Industry Association, Korea Advanced food Research Institute) ;
  • Kim, Kee-Hwan (Division of Biological Science, Wonkwang University) ;
  • Park, Jong-Kun (Department of Food and Nutrition, Wonkwang University) ;
  • Hwang, Eun-Hee (Division of Biological Science, Wonkwang University)
  • 이형자 (한국식품공업협회 식품연구소) ;
  • 김기환 (원광대학교 식품영양학과) ;
  • 박종군 (원광대학교 생명과학부) ;
  • 황은희 (원광대학교 식품영양학과)
  • Published : 2008.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study analyzes the apoptosis of HeLa cells to see if we can use the Artemisia capillaris Thunberg for the prevention of chronic degenerative diseases. We used the HeLa cells to see what effects the A. capillaris Thunberg had on apoptosis of the cancer cells. We checked the cell activity, cell morphological change, DNA fragmentation, and DNA content after administering 0, 100, 500, 1000, and $2000{\mu}g/ml$ methanol, ethyl acetate, n-butanol extract of the A. capillaris Thunberg. As for the cell viability, the increase of concentration of methanol and ethyl acetate decreased the survival rate of the cell, but the phenomenon was much weakened in n-butanol extract and was not observed in aqueous extract. The higher the density of the methanol, ethyl acetate, n-butanol and aqueous extract was, the lower the survival rate of the HeLa cell was. These extracts obstructed the cell cohesion and caused the blebbing of he cell membrane and fragmentation of the nucleus, both of which are symptoms of apoptosis. Laddering-pattern DNA fragmentation was observed in the groups that were treated with the $1000{\mu}g/ml$ and $2000{\mu}g/ml$ of methanol extract. The DNA content of the cells apoptosis measured by fluorescent-activated cell sorter (FACS) increased as the density of the methanol, ethyl acetate and butanol extract increased. The result of the study shows that A. capillaris Thunberg fosters the apoptosis of HeLa cells, which suggests that the A. capillaris Thunberg has a great potential value as food additives, medicinal supplements for patients with chronic diseases, and preventive measures against cancer.

사철쑥 (Artemisia capillaris Thunberg)이 암세포의 세포고사에 미치는 영향을 보기 위하여 인체 자국경부 상피암 세포주인 HeLa 세포를 이용하여 사철쑥의 메탄올, 에틸아세테이트, 부탄올 추출물의 농도를 각각 0, 100, 500, 1,000 및 $2,000{\mu}g/ml$로 처리하여 세포활성도, 세포형태 변화, DNA분절, DNA 함량을 알아보았다. 세포활성도는 사철쑥의 메탄올, 에틸아세테이트 추출물이 농도에 비례하여 HeLa세포생존율을 감소시켰지만 부탄올 추출물에서는 그 정도가 약하였고 물 추출물에서는 거의 관찰되지 않았다. 사철쑥의 메탄올, 에틸아세테이트, 부탄올과 물 추출물은 각 추출물의 농도에 의존적으로 배양된 HeLa 세포에 세포부착을 방해하고 세포막의 blobbing 및 핵의 분절화 등 세포고사의 특징들을 나타냈다. 사다리형 DNA 분절현상은 사철쑥의 메탄올 추출물 $1,000{\mu}g/ml$, $2,000{\mu}g/ml$ 처리군에서 관찰되었다. 유세포분석기 (FACS)를 이용하여 측정한 고사세포의 DNA 함량은 메탄올과 에틸아세테이트, 부탄올 추출물이 농도 의존적으로 증가하였다. 이상의 결과는 사철쑥이 암세포의 세포고사를 증가시킴으로서 암 억제 작용이 있을 것을 시사해 준다고 할 수 있다.

Keywords

References

  1. 한국생명과학교수회 편저. 본초학, 아카데미서적; 1994. p.366
  2. 이성우. 고려이전의 한국 식생활사. 향문사; 1986. p.388
  3. 김재길. 원색천연약물대사전. 남산당 1992;64:79
  4. 신민교. 임상본초학. 영림사; 1994. p.602
  5. 이창복. 대한식물도감. 향문사; 1985. p.753-761
  6. Kim KS, Lee MY. Effects of Artemisia selengensis methanol extract on ethanol-induced hepatotoxicity in rat liver. J Korean Soc Food Sci Nutr 1996;25(4):581-587
  7. Bown D. Encyclopaedia of herbs and their uses. Darling Kindersley. London; 1995. p.230
  8. Lee GD, Kim JS, Bae JO, Yoon HS. Antioxidative effectiveness of water extract and ether extract in wormwood (Artemisia montana Pampan). J Korean Soc Food Sci Nutr 1992; 21(1): 17-22
  9. Hwang YK, Kim DC, Hwang WI, Han YB. Inhibitory effect of Artemisia princeps Pampan extract on growth of cancer cell lines. Korean J Nutr 1998; 31(4): 799-808
  10. Nam SM, Kim JG, Ham SS, Kim SJ, Chung ME, Chung CK. Effects of Artemisia iwayomogi extracts on antioxidant enzymes in rats administered benzo (${\alpha}$ )pyrene. J Korean Soc Food Sci Nutr 1999; 28(1): 199-204
  11. Lee SY. Use and perspective views of oriental herbs in food industury. Korean J Food & Nutr 2005; 5(3): 21-26
  12. Park JC, Lee ST. Functional food and bioactive constituents from oriental medicine resources. Korean J Food Indus and Nutr 2000; 5(3): 27-36
  13. Tariq MM, Mossa JS, Al-Yahya MA, Parmar NS, Ageel AM. Evaluation of Artemisia inculta for anti-inflammatory activity in rats. Am J Clin Med 1987; 15(3-4): 127-132 https://doi.org/10.1142/S0192415X87000163
  14. Twaij HA, Al-Badr AA. Hypoglycemic activity of Artemisia herba alba. J Ethnopharmacol 1994; 43(3): 167-171 https://doi.org/10.1016/0378-8741(94)90038-8
  15. Park SK, Park JC. Antimicrobial activity of extracts and coumaric acid isolated from Artemisia princeps var. orientalis. Korean J Biotechnol Bioeng 1994; 9(5): 506-511
  16. Park KY, Ha JO, Yu YB, Lee JH. Antimutagenic effect of some Artemisia species. Korean J Pharmacogn 1996; 27(2): 96-100
  17. Ham SS, Chung CK, Lee JH, Choi KP, Jung SW, Kim EJ. Antimutagenicity and cytotoxicity of Artemisia iwayomogi Kitamura extracts. J Korean Soc Food Sci Nutr 1998; 27(1): 157-162
  18. Koshihara Y, Neichi T, Muroda S, Lao A, Fujumoto Y, Tatsuno T. Selective inhibition of 5-lipoxygenase by natural compounds isolated from Chinese plants (Artemisia rubripes Nakai). FEBS Lett 1983; 158(1): 41-49 https://doi.org/10.1016/0014-5793(83)80672-3
  19. Cohen JJ. Apoptosis the physiologic pathway of cell death. Hosp Pract(Off Ed) 1993; 28(12): 35-43
  20. Arends MJ, Wyllie AH. Apoptisis mechanism and roles in pathology. Int Rev Exp Pathol 1991; 32: 223-254
  21. Lee HJ, Hwang EH, Yu HH, Song IS, Kim CM, Kim MC, Hong JH, Kim DS, Han SB, Kang KJ, LEE EJ, Chung HW. The analysis of nutrients in Arthemisia Capillalis Thunberg. J Korean Soc Food Sci Nutr 2002; 31(3): 361-366 https://doi.org/10.3746/jkfn.2002.31.3.361
  22. Lee HJ, Hwang EH. Efect of Arthemisia Capillalis Thunberg on Plasma and Liver Lipid Metabolsm in rats. Korean J Nutr 2002; 35(4): 421-430
  23. Giannoni E, Cirri P, Paoli P, Fiaschi T, Camici G, Manao G, Raugei G, Ramponi G. Acylphosphatase is a strong apoptosis inducer in HeLa cell line. Mol Cell Biol Res Commun 2000; 3(5): 264-270 https://doi.org/10.1006/mcbr.2000.0228
  24. Ferrera R, Larese A, Berthod F, Guidollet J, Rodriguez C, Dureau G, Dittmar A. Quantitative reduction of MTT by hearts biopsies in vitro is an index of viability. J Mol Cell Cardiol 1993; 25(9): 1091-1099 https://doi.org/10.1006/jmcc.1993.1121
  25. Brown DG, Sun XM, Cohen GM. Dexamethasone-induced apoptosis involves cleavage of DNA to lage fragments prior to internucleosomal fragmentation. J Biol Chem 1993; 268(5): 3037-3039
  26. Yano H, Mizoguchi A, Fukuda K, Haramaki M, Ogasaware S, Momosaki S, Kojiro M. The herbal medicine sho-saiko-to inhibirs proliferation of cancer cell lines by inducing apoptosis and arrest at the G0/G1 phaes. Cancer Res 1994;54:448-454
  27. Kastan MB, Onyekwerw O, Sidransky D, Vogelstein B, Craig RW. Participation of p53 protein in the cellular response to DNA damage. Cancer Res 1991;561:6304-6311
  28. Garc JF, Piris MA, Lloret E, Orradre JL, Murillo PG, Martez JC. p53 expression in CMV-infected cells association with the alterative expression of the p53 transactivated genes p21/WAF1 and MDM2. Histopathology 1997;30(2):12-125
  29. Hu YQ, Tan RX, Chu MY, Zhou J. Apoptosis in human Hepatoma Cell Line SMMC-7721 induced by water-soluble macromolecular components of Artemisia capillaris Thunberg. Jpn J Cancer Res 2000;91(1):113-117 https://doi.org/10.1111/j.1349-7006.2000.tb00867.x
  30. Hahm KB, Kim JH, You BM, Kim YS, Cho SW, Yim H, Ahn BO, Kim WB. Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats. Pancreas 1998;17(2):153-157 https://doi.org/10.1097/00006676-199808000-00007
  31. Kim YS, Lee JH, Kim MN, Lee WG, and Kim JO. Volatile flavor compounds from raw mugwort leaves and parched mugwort tea (in Korea). J Korean Soc Food Sci Nutr 1994; 23(2): 261-267