• Journal of Korean Society of Forest Science
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• 제99권2호
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• pp.251-257
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• 2010

Influences of environment (indicated by plant associations) and forest management practices on the distribution of Armillaria spp. and genets (vegetative clones) were investigated. A total of 142 isolates of Armillaria was collected from various host trees on pristine and managed sites (thinned and/or fertilized) growing in relatively wet and dry environments in eastern Washington, U.S.A. The incidence of Armillaria spp. was significantly higher in the relatively wetter sites than the relatively drier sites, as indicated by plant associations. However, no differences in Armillaria occurrence were found among different forest management practices (control vs. thinned vs. thinned and fertilized) within both wetter and drier sites. Incidence of Armillaria was significantly different among conifer and shrub species. The highest proportion with Armillaria was found on grand fir (Abies grandis). Based on pairing tests and rDNA sequencing, the 142 isolates were comprised in a total of 20 genets representing three Armillaria species. More diverse Armillaria spp. were found in both relatively wetter and relatively drier sites within the undisturbed control plots, compared to plots disturbed by forest management practices. The results from this study provide baseline information toward understanding how environment and forest management practices influence incidence and diversity of Armillaria species and genets.

• The Korean Journal of Mycology
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• 제23권1호통권72호
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• pp.61-70
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• 1995

The genus Armillaria is important because they produce Gastrodia tubers. Seventy two isolates of Armillaria were obtained from fruit bodies grown on decayed wood in Korea. Twenty four isolates from Pinus koraiensis were identified as A. ostoyae. Two isolates from G. elata growing in the field were identified as A. mellea. Seven isolates from Acer ginnala and Quercus spp. were identified as A. tabescens. Thirty nine isolates were identified as A. gallica. Armillaria gallica was isolated from Quercus spp., Ainus japonica, Vitis amurensis and Prunus sargentii. Armillaria spp. isolates were divided into four groups based on the cultural characteristics. Group II (A. gallica KNU-A110) was better than the other groups for mycelial growth and rhizomorph formation. Isolate KNU-A110 proved to be good for production of G. elata tubers. This fungus forms mycelial fan in the plant tissue and rhizomorphs in contact with G. elata tubers. Gastrodia spp. was found in thirteen sites in Kangweon province in Korea. The plants were divided into three different kinds based on stem color. Plants with stems of brownish orange and greyish yellow were identified as G. elata, and those with greyish green colored stems were identified as G. gracilis. Gastrodia was collected mainly from humus soils rich in leaf debris, and slopes facing south from mid-May to mid-July. Once the new tubers are formed from the ancestry tuber, the ancestry tuber begins to decay. The offspring tuber, apparently gaining nutrients through rhizomorphs, begins to grow in length and slowly to enlarge. It takes three years for the offspring tuber to become ancestry tuber.

• Journal of Mushroom
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• 제10권3호
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• pp.143-149
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• 2012

Armillaria spp are well known as a symbiotic fungus with Gastrodia elata. This study was carried out to identify and analyze the genetic relationships among 83 strains of Armillaria spp.. The amplified internal transcribed spacer(ITS) region of the rDNA was about 500~750 bp long and identified by 9 strains; A. mellea, A. tabescens, A. ostoyae, A. gallica, A. novae-zenlandia, A. cepistipes, A. nabsnona, A. gemina, A. sinapina. Sequence analysis showed that 52% of strains were different with original identification. A. gallica, A. cepistipes and A. gemina were so close phylogenetic relationship, that was difficult to classify using ITS region. In A. gallica, 12 strains including ASI10104 were showed a close phylogenetic relationship with A. gallica, A. cepistipes and A. gemina. ASI10017 and ASI10114 were classified as the A. sinapina group, ASI10045 was the A. borealis group, ASI10002 and ASI10025 were the A. ostoyae group. So more studies need for more accurate identification and determine the phylogenetic relationships of Armillaria spp.

• Korean Journal Plant Pathology
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• 제10권4호
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• pp.261-269
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• 1994

One hundred and ninety two isolates of Armillaria were obtained from mycelial fans on infected hosts, rhizomorphs, and single basidiospores or trauma tissue of fruiting bodies. Mating tests showed that two of these isolates were A. mellea, eight were A. tabescens, 20 were A. ostoyae, and 162 were A. gallica. Armillaria ostoyae was mainly isolated from Pinus koraiensis and Qurecus spp., A. tabescens from fruiting bodies on Pinus densiflora and Qurecus spp., and A. gallica from many tree species but not Pinus koraiensis. Armillaria mellea, A. gallica, A. ostoyae and A. tabescens showed distinct protein banding patterns. Mycelial growth and rhizomorph formation was good on basal medium with ethanol added. A. gallica and A. mellea formed many rhizomorphs, but A. ostoyae did not. A. gallica showed the best rhizomorph formation on media with tannic acid and ethanol, but a. mellea formed the most rhizomorphs on gallic acid. Rhizomorphs showed monopodial branching for A. gallica and dichotomous branching for A. ostoyae. Fruiting bodies. formed in the laboratory on sawdust media most abundantly by A. tabescens. In nature, fruit body formation by A. tabescens was from early to mid August. A. ostoyae and A. gallica fruit bodies were formed from early August to late October. While there are common names in Korea for A. mellea and A. tabescens, such as mulberry mushroom relative, no common names are available for A. gallica and A. ostoyae. Therefore, we refer to a. gallica as the Gastrodia mushroom because it has been used to produce Gastrodia and A. ostoyae as the Korean pine mushroom because it is frequently found as mushrooms on Korean pine.

• The Korean Journal of Mycology
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• 제24권2호통권77호
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• pp.127-134
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• 1996

Armillaria isolates (KNU-A110, KNU-A234, KNU-A1022 and KNU-A1030) were excellent isolates for producing Gastrodia tuber in farm cultivation. Depth of soil between $10\;cm{\sim}19\;cm$ was favorable for producing Gastrodia tuber and rhizomorph at cultivated area. Eighty nine isolates were collected from 5 countries; 16 from Japan, 22 from USA, 26 from France, 4 from Africa and 21 from Korea. Mycelial fan of most isolates were better formed on basal medium with lemon extract than without lemon.A. mellea (KNU-A997) were strongly pathogenic to Gastrodia but A. gallica (KNU-A110) were excellent symbiotic to Gastrodia tuber. Mycelial growth were good on basal medium containing $0.25%{\sim}0.5%$ ethanol and sawdust spawn added wheat bran and corncob. Liquid culture inoculation were not only fast growth of mycelium but also reduction of contamination.