• The Korean Journal of Mycology
• /
• v.20 no.2
• /
• pp.126-133
• /
• 1992

Using the soils containing several arbuscular mycorrhizae, the degrees of infection on the plant roots were measured with the different level of phosphate added on pot cultures. Infection on the plant roots was independent of the phosphate level for the roots of sorghum, but formation of arbuscular mycorrhizae in the roots was inversely related to the growth of soybean roots. It was concluded that infection of arbuscular mycorrhizae would be related to the phosphate level in the soils. Plants, themselves, were considered to control the infection of arbuscular mycorrhizae under the environments of soils, especially amount of phosphate.

• The Korean Journal of Mycology
• /
• v.20 no.2
• /
• pp.134-143
• /
• 1992

The association of soil environments and sporulation of arbuscular mycorrhizae was investigated using pot cultures. Increased arbuscular mycorrhizal spores as well as the better growth of the host plants were observed when sorghum (Sorghum bicolor) was treated with different soil conditions using several fertilizers with different concentrations. For to five fold increase of sporulation of arbuscular mycorrhizae was noticed depending on the mycorrhizal species. Although there were some differences between the four arbuscular mycorrhizae species and the conditions of soil environments, maximum populations of spores were reached at about 30-40 days after cultivation. The populations of four arbuscular mycorrhizal species was individually fluctuated. Also, the growth rate of host plants were different from the fertilizers over 2-3 times, but the increase of spores were not influenced by it.

• The Korean Journal of Mycology
• /
• v.17 no.4
• /
• pp.214-222
• /
• 1989

Of the 103 species (41 families) sampled from two limestone bites in Korea, 98 species (95.1%) contained VA mycorrhizae. No mycorrhizal structures were observed in the other 5 species. The nonmycorrhizal species were members of the following 4 families: Equisetaceae, Davalliaceae, Amaranthaceae and Ericacene. Of the 124 species (51 families) sampled from two different nonlimestone sites, 99 species (79.8%) were found to contain VA mycorrhizae and no mycorrhizal structures were observed in the other 25 species. The nonmycorrhizal species were members of the following 16 families: Equisetaceae, Pteridacaea, Davalliaceae, Cyperaceae, Commelinaceae, Polygonaceae, Chenopodiaceae, Phytolaceaceae, Amaranthaceae, Aizoaceae, Portulacaceae, Caryophyllaceae, Fumariaceae, Cruciferae, Ericaceae and Rubiaceae.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.2
• /
• pp.181-186
• /
• 1997

Since volcanic ash soils in Cheju island have high capacities of adsorption and immobilization of phosphate, a relatively high rate of P application has been recommended in citrus orchards for many years and such a large amount of P application could be problematic both in agricultural and environmental point of view. The objective of this study was to test whether arbuscular-mycorrhizae can be used to improve P availability in Cheju citrus orchard soils. Soil, root and leaf samples were taken from 14 citrus orchards of different location and soil texture. Mycorrhizal spore distribution in the soils, mycorrhizal infection ratio on the citrus roots, and mineral nutrients in leaf samples were determined. Numbers of mycorrhizal spore were in the range of $9,000{\sim}40,000/100g$ soil. The population level was not correlated with any of the soil characteristics examined. Mycorrhizae were found in all of the examined orchards and root infection ratio varied between $14{\sim}60%$. The mycorrhizae infection ratio differed substantially in different soils. Although root infection was high at soils with low extractable P level, it was not significantly correlated with other soil factors measured. Since a positive correlation was observed between leaf P concentration and root infection, enhancement of P uptake seemed to be associated with mycorrhizal infection. These results indicate that mycorrhizae could be a useful method to reduce P applications in Cheju citrus orchards.

• Korean Journal of Environmental Agriculture
• /
• v.18 no.3
• /
• pp.287-291
• /
• 1999

In order to examine the possible use of rock phosphate as P source, tomato seedlings with or without inoculation of arbuscular mycorrhizal fungi were grown in the pots of sterile volcanic ash soil from Cheju island with two levels of phosphorus (100 and 200 mg/kg) supplied either as fused or as rock phosphate. After three months of culture, plant dry weight, P and other nutrient uptake, root colonization and spore density in the soil were determined. Treatments of rock phosphate of both levels resulted in the significantly depressed plant growth in comparison to the treatments of fused phosphate, likely due to lower P availability in soil with rock phosphate. Mycorrhizal fungi inoculation increased the dry weight of plant at 200 mg/kg level of both fused and rock phosphate. Root infection and sporoulation were reduced in rock phosphate treatments. Nitrogen, K, Ca and Mg contents in plants were not significantly different at all treatments. As a P source, rock phosphate in combination with mycorrhizae was not satisfactory for optimum plant growth at $100{\sim}200\;mg/kg$ levels in Cheju volcanic ash soil.

• Journal of Ginseng Research
• /
• v.19 no.1
• /
• pp.73-76
• /
• 1995

Newly sprouted American ginseng (Panax quinquefolium L.) seedlings were transplanted to forest pots with mycorrhizae-infested soil and grown in screenhouse for 2 years. Growth patterns, mortality rate and fresh root weight were investigated. Plants in VAM soil had lower mortality rales than control. In soils infested with two species of mycorrhizal fungi (Glomu deseyicola, frappe, Bloss and Merge and G. intraradices, Schenck and Smith), 28-35% of plants produced 3-prongs in the second season and significantly increased fresh root weight by 41 to 43%.

• The Korean Journal of Mycology
• /
• v.21 no.1
• /
• pp.38-50
• /
• 1993

Four plant (Sorghum bicolor, Cassia mimosoides var. nomame, Sesamum indicum and Glycine soja) were cultivated at the pots including the soils containing arbuscular mycorrhizal fungi and were also investigated with the colonizations and productions of arbuscular mycorrhizal fungi. Whereas the colonizations of arbuscular mycorrhizal fungi continuosly increased on the roots until 50 days, the productions of arbuscular mycorrhizal fungal spores were fluctuated with the terms of 30 days after inoculated. This indicated that the colonizations on the roots were not correlated with productions of arbuscular mycorrhizal fungal spores. Also, the various soils collected were applied to this technique by using pot cultures. Out of 82 various soils collected, the spore productions of arbuscular mycorrhiaze were observed only from 42 soils. The spores cultured under artificial conditions were identified to 15 species with four genera. The spore productions of arbuscular mycorrhizal fungi using this technique would be considered to be related to the soil pH: The spore productions were found in the low pH for the species of Acaulospora and Glomus, the those near pH 7.6 for the species of some Glomus, Scutellospora and Gigaspora.

• Mycobiology
• /
• v.30 no.3
• /
• pp.146-153
• /
• 2002

Two types of mycorrhizae, orchid(OM) and arbuscular mycorrhizae(AM), were observed in the cortical cells of Botrychium ternatum roots. The vesicles or arbuscules of AM fungi were examined and the fresh or digestive pelotons by other species of basidiomycetes were also observed in the roots under light microscope. These symbioses were, as the genomic DNAs extracted from roots of B. ternatum reacted with the specific primers, confirmed with PCR technique, being added to more strong evidences. These discoveries were rarely happened in the roots, especially a fern in nature. OM was observed in the roots of B. ternatum collected from the nationwide areas, whereas AM was only in the roots of B. ternatum collected from Chung-Buk areas. It is speculated that OM are associated with the nitrogen cycle in Islands and the growth of B. ternatum in the inland of Central Korea is related to both the phosphate and nitrogen cycle in the nature. The results suggest that B. ternatum is a typical species with two types of mycorrhizae under various growing conditions.

• The Korean Journal of Ecology
• /
• v.13 no.1
• /
• pp.1-8
• /
• 1990

Effects of mycorrhizal infection on the growth of bell pepper (Capsicum annuum) and corn (Zea mays) seedlings have been studied by comparing plants grown in sterilized soil/sand mixtures to plants grown in sterilized soil/sand mixtures with topping the original non-sterile field soil. The original nonsterile field soil, which were taken from the bell pepper field, contained a high level of endmycorrhizal spores. After seven weeks, the shoot height of inoculated plants was increased by 110% in bell pepper, and 90% in corn compared with the control plants. The average above-ground biomass of inoculated plant was increased by 88% in bell pepper and 71% in corn compared with the control plants. The shoot-root ratios in bell pepper and corn were 2.7 and 1.8 for the control plants, and 4.3 and 2.7 for the treatment plants, respectively. Phosphorus level in inoculated plant was higher than that of the control plant. However, nitrogen contents were similar between the control and the treatment plants. The control plants didi not form vesicular-arbuscular mycorrhizae during the experimental period.

• Mycobiology
• /
• v.39 no.2
• /
• pp.79-84
• /
• 2011

Nuclear distribution within the extra-radical fungal structures and during spore production in the arbuscular mycorrhizae fungus Glomus intraradices was examined using an in vitro monoxenic culture system. A di-compartmental monoxenic culture system was modified using a nitrocellulose membrane and a coverglass slip for detailed observations. Nuclear distribution was observed using the fluorescent DNA binding probes SYBR Green I and DAPI. Both septate and non-septate mycelial regions were observed, but cytoplasmic contents were only found within non-septate mycelia. Nuclear fluorescent staining revealed that the non-septate hyphal region contained nuclei only with cytoplasm, and that nuclear distribution was limited by septa. Swollen hyphal bodies were often associated with septate and empty-looking hyphae. Cytoplasmic contents filled the swollen hyphal body from the non-septate hyphal region following removal of the septa. As a consequence, the swollen body developed into a new spore. These observations provide understanding about the distribution of AM fungal nuclei within extra-radical mycelia and during spore formation. The results suggest a mechanism by which the development of a cytoplasm-containing mycelium is controlled by the formation or removal of septa to efficiently maintain and proliferate essential contents. This mechanism may provide a survival strategy to the fungus.